BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0484.0002.p (292 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5T5.1 B3K5T5_9BACI 4-hydroxyphenylacetate 3-hydroxylase 324 5e-87 C4CLT1.1 C4CLT1_9CHLR 4-hydroxyphenylacetate 3-monooxygenase, ox... 224 8e-57 A7GMD9.1 A7GMD9_BACCN 4-hydroxyphenylacetate 3-monooxygenase, ox... 217 9e-55 C2WFJ6.1 C2WFJ6_BACCE 4-hydroxyphenylacetate-3-hydroxylase 214 5e-54 A7Z0Y6.1 A7Z0Y6_BACA2 YoaI 208 5e-52 >B3K5T5.1 B3K5T5_9BACI 4-hydroxyphenylacetate 3-hydroxylase Length = 490 Score = 324 bits (831), Expect = 5e-87, Method: Compositional matrix adjust. Identities = 155/285 (54%), Positives = 201/285 (70%), Gaps = 3/285 (1%) Query: 8 VFPSPFPSMQEQDNPYAFAFAVPNDLEGMTFICRESYSGSSHYDHPLSSRFEEMDAMVIF 67 VFPSP P++ NP AFAFAVPNDL GMTFICRESY +S YD+PLS+R+EEMD +VIF Sbjct: 206 VFPSPSPTLFHDVNPTAFAFAVPNDLPGMTFICRESYGAASSYDYPLSARYEEMDVLVIF 265 Query: 68 DHVFIPRYRIFYLGNEEIGERLFSEGNFHSHAGHQVLTRYIGKTEFLLGLISKLSDEXXX 127 DHV +P RIFY G+E RLF E FH+H HQ+ TRYI KTEF+LGL+ L+DE Sbjct: 266 DHVLVPHDRIFYYGDEAYAGRLFGESRFHTHIAHQMATRYIAKTEFMLGLLESLADE--Q 323 Query: 128 XIALEAVTIERISRILTMLENLRSLRLASEIMAEPDSKGYYVPAQKPLIAATMQYSSFYQ 187 + LE I +S+I+ LE ++LRLASE+ A D GY+VP+ PL A++M + FY Sbjct: 324 NVGLEPQMIANVSKIIAYLETFKALRLASELGASRDKFGYFVPSAGPLTASSMLFPHFYP 383 Query: 188 EMLGMLQDISSSSLVMLPSETDLESDAGDFIRLYLKGQDTSACDRISLFRLTWELGVGPF 247 EM+ M+Q + SS L+M+PSE D +SD+G ++ YL+G T A DRI+LFRL WELG G F Sbjct: 384 EMVEMIQLLGSSGLIMIPSEMDFQSDSGPYLNQYLRGSTTEARDRIALFRLAWELGAGSF 443 Query: 248 GGRQKQFERFFFGNTRTLTSRMYNGYSL-DKYKAMIDEFLHRGET 291 GGRQ QFERFFFGN +T+ RMYN + D+ + I +F+ + +T Sbjct: 444 GGRQTQFERFFFGNAQTVAYRMYNQFEQHDQLRNRIQDFIAKNDT 488 >C4CLT1.1 C4CLT1_9CHLR 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component Length = 491 Score = 224 bits (570), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 114/286 (39%), Positives = 168/286 (58%), Gaps = 7/286 (2%) Query: 8 VFPSPFPSMQEQDNPYAFAFAVPNDLEGMTFICRESYS-GSSHYDHPLSSRFEEMDAMVI 66 VFPS E+D PY FAFA+P D G+ FICRES+ G SH+DHPL SRFEEMDA+V+ Sbjct: 203 VFPSTVLRGTEEDRPYTFAFAIPCDTPGLKFICRESFDIGRSHFDHPLGSRFEEMDAVVV 262 Query: 67 FDHVFIPRYRIFYLGNEEIGERLFSEGNFHSHAGHQVLTRYIGKTEFLLGLISKLSDEXX 126 FD V +P R+F G+ E+ +F+E N H HQV+ + + K EF+ G+ + ++D Sbjct: 263 FDDVLVPWERVFLYGDVELANGVFAETNAVVHMAHQVVVKNVAKAEFIFGIAALIADR-- 320 Query: 127 XXIALEAV--TIERISRILTMLENLRSLRLASEIMAEPDSKGYYVPAQKPLIAATMQYSS 184 I ++ E+++ I+ LE +R+ ASE A D G PA PL AA + Sbjct: 321 --IGIDQFQHVQEKLAEIIIDLEAMRAFLRASEADAAVDQWGLMTPAWGPLNAARNLFPR 378 Query: 185 FYQEMLGMLQDISSSSLVMLPSETDLESDAGDFIRLYLKGQDTSACDRISLFRLTWELGV 244 Y M+ ++Q + +S L+ +P+E D+ S A I YL+G+++ A +R+ LFRL W+ + Sbjct: 379 LYPRMIEIIQILGASGLMAIPTEADMRSPARAMIDRYLQGRNSDAYERVKLFRLAWDAAL 438 Query: 245 GPFGGRQKQFERFFFGNTRTLTSRMYNGYSLDKYKAMIDEFLHRGE 290 FG RQ +ERFFFG+ + +Y Y+ + YK + FL R E Sbjct: 439 SSFGSRQVLYERFFFGDPVRMAGALYTSYNKEPYKERVQAFLDRVE 484 >A7GMD9.1 A7GMD9_BACCN 4-hydroxyphenylacetate 3-monooxygenase, oxygenase subunit Length = 490 Score = 217 bits (553), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 111/284 (39%), Positives = 169/284 (59%), Gaps = 3/284 (1%) Query: 8 VFPSPFPSMQEQDNPYAFAFAVPNDLEGMTFICRESYS-GSSHYDHPLSSRFEEMDAMVI 66 VFPS E++NPYA+AF++PN G+ FICRES+ G SH+DHPL SRFEEMD +V+ Sbjct: 203 VFPSTLLKQSEEENPYAYAFSIPNHTPGLKFICRESFDYGKSHFDHPLGSRFEEMDTIVV 262 Query: 67 FDHVFIPRYRIFYLGNEEIGERLFSEGNFHSHAGHQVLTRYIGKTEFLLGLISKLSDEXX 126 FD V +P R+F LG+ + + ++E N H HQV+++ I KTEF+LG++ +L E Sbjct: 263 FDDVVVPWNRVFALGDVGVCNQAYNESNAVVHMTHQVVSKNIAKTEFVLGIL-QLMVETI 321 Query: 127 XXIALEAVTIERISRILTMLENLRSLRLASEIMAEPDSKGYYVPAQKPLIAATMQYSSFY 186 + V E++S ++ LE +++ A+E A+ D G P PL A Y Y Sbjct: 322 NIGQFQHVQ-EKMSEVIMALETVKAYVTAAEANAKLDKWGIMTPDFTPLNVARHYYPKIY 380 Query: 187 QEMLGMLQDISSSSLVMLPSETDLESDAGDFIRLYLKGQDTSACDRISLFRLTWELGVGP 246 ++Q + +S L+ +PSE+D +S+ + YL+ + A +R+ L+RL W++ + Sbjct: 381 PRFSEIMQLLGASGLMAIPSESDFQSELRPDLDKYLQAANGDAYNRVKLYRLAWDVCMSA 440 Query: 247 FGGRQKQFERFFFGNTRTLTSRMYNGYSLDKYKAMIDEFLHRGE 290 FG RQ +ERFFFG+ + S +YNGY Y + EFL+R E Sbjct: 441 FGTRQTLYERFFFGDPVRMASALYNGYDKQVYVDRVKEFLNRSE 484 >C2WFJ6.1 C2WFJ6_BACCE 4-hydroxyphenylacetate-3-hydroxylase Length = 490 Score = 214 bits (546), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 112/284 (39%), Positives = 169/284 (59%), Gaps = 3/284 (1%) Query: 8 VFPSPFPSMQEQDNPYAFAFAVPNDLEGMTFICRESYS-GSSHYDHPLSSRFEEMDAMVI 66 VFPS E++NPYA+AF++PN+ G+ FICRES+ G SH+DHPL SRFEEMD +V+ Sbjct: 203 VFPSTLLKQSEEENPYAYAFSIPNNTPGLKFICRESFDYGKSHFDHPLGSRFEEMDTIVV 262 Query: 67 FDHVFIPRYRIFYLGNEEIGERLFSEGNFHSHAGHQVLTRYIGKTEFLLGLISKLSDEXX 126 FD V +P R+F LG+ + + ++E N H HQV+++ + KTEF+LG++ +L E Sbjct: 263 FDDVVVPWNRVFALGDVGVCNQAYNESNAVVHMTHQVVSKNVAKTEFVLGIL-QLMVETI 321 Query: 127 XXIALEAVTIERISRILTMLENLRSLRLASEIMAEPDSKGYYVPAQKPLIAATMQYSSFY 186 + V E++S ++ LE +++ A+E A+ D G P PL A Y Y Sbjct: 322 NISQFQHVQ-EKMSEVIMALETVKAYVAAAEANAKMDKWGIMTPDFTPLNVARHYYPKIY 380 Query: 187 QEMLGMLQDISSSSLVMLPSETDLESDAGDFIRLYLKGQDTSACDRISLFRLTWELGVGP 246 ++Q + +S L+ +PSETD SD + YL+ + A +R+ L+RL W++ + Sbjct: 381 PRFSEIMQLLGASGLMAIPSETDFNSDLRPDLDKYLQSANGDAYNRVKLYRLAWDVCMSA 440 Query: 247 FGGRQKQFERFFFGNTRTLTSRMYNGYSLDKYKAMIDEFLHRGE 290 FG RQ +ERFFFG+ + S +YNGY +Y + EFL R E Sbjct: 441 FGSRQTLYERFFFGDPVRMASALYNGYDKQEYVDRVKEFLSRSE 484 >A7Z0Y6.1 A7Z0Y6_BACA2 YoaI Length = 373 Score = 208 bits (529), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 110/272 (40%), Positives = 164/272 (60%), Gaps = 17/272 (6%) Query: 24 AFAFAVPNDLEGMTFICRESYS-GSSHYDHPLSSRFEEMDAMVIFDHVFIPRYRIFYLGN 82 AFAF++P++ +G+ FICRES+ G S +D+PLSSR+EEMD++V+FDHV +P R+F+ N Sbjct: 108 AFAFSIPSNTKGVKFICRESFVLGKSSFDYPLSSRYEEMDSIVVFDHVLVPWERVFFYNN 167 Query: 83 EEIGERLFSEGNFHSHAGHQVLTRYIGKTEFLLGLISKLSDEXXXXIALEAVTI------ 136 E + +F++ A HQV+TR I KTEFLLGL L +EA+ + Sbjct: 168 YEAAFDFNMKSSFNAFAFHQVITRQIVKTEFLLGLAQTL---------VEAIDVGEYQHI 218 Query: 137 -ERISRILTMLENLRSLRLASEIMAEPDSKGYYVPAQKPLIAATMQYSSFYQEMLGMLQD 195 +++ I+T LE +++L SE AE D GY P+ PL + + Y ++Q Sbjct: 219 QAKMAEIITGLETMKALLEKSENDAETDEWGYMRPSVTPLRVVSAIFPDIYPRFTEIIQL 278 Query: 196 ISSSSLVMLPSETDLESDAGDFIRLYLKGQDTSACDRISLFRLTWELGVGPFGGRQKQFE 255 I +S +V LP+E SD + YL+ + +A DR+ +FRL W+L + PFG RQ Q+E Sbjct: 279 IGASGMVTLPTEKAFRSDIRGDLDQYLQAAELNAEDRVKVFRLAWDLTMSPFGTRQTQYE 338 Query: 256 RFFFGNTRTLTSRMYNGYSLDKYKAMIDEFLH 287 R+FFG+ LTS +Y Y ++ A+I EFLH Sbjct: 339 RYFFGDPVRLTSHLYRSYPKEEQTAVIKEFLH 370 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,647,941,175 Number of extensions: 64826888 Number of successful extensions: 126079 Number of sequences better than 1.0e-05: 344 Number of HSP's gapped: 125457 Number of HSP's successfully gapped: 344 Length of query: 292 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 158 Effective length of database: 1,798,280,193 Effective search space: 284128270494 Effective search space used: 284128270494 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)