BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0484.0001.p (190 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5T5.1 B3K5T5_9BACI 4-hydroxyphenylacetate 3-hydroxylase 234 3e-60 A9U7F1.1 A9U7F1_PHYPA Predicted protein (Fragment) 182 2e-44 A7GMD9.1 A7GMD9_BACCN 4-hydroxyphenylacetate 3-monooxygenase, ox... 172 9e-42 C2WFJ6.1 C2WFJ6_BACCE 4-hydroxyphenylacetate-3-hydroxylase 169 2e-40 B1HUK6.1 B1HUK6_LYSSC Pyoverdin chromophore biosynthetic protein 156 8e-37 >B3K5T5.1 B3K5T5_9BACI 4-hydroxyphenylacetate 3-hydroxylase Length = 490 Score = 234 bits (597), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 100/159 (62%), Positives = 133/159 (83%) Query: 6 MAVKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQM 65 + +K+GK+Y ERI+RQ IN+WY+G++I+ PLS HPAF G+++TQAE+YDMQ + ++EQM Sbjct: 4 VPIKSGKQYIERIDRQNINLWYKGELIQGPLSKHPAFQGIIQTQAELYDMQGEACYIEQM 63 Query: 66 TFQSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAA 125 T+ S GER GLSFL PR+ +DL +RR+MMELW ++H+GFLGRSPDYMNT +MSLYTAA Sbjct: 64 TYPSPDTGERVGLSFLPPRNAKDLQKRRLMMELWAKRHNGFLGRSPDYMNTAIMSLYTAA 123 Query: 126 HLLQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 +L+E+NP+Y DNL+ YY YCR DITLSHAFIQP+A + Sbjct: 124 DILEEHNPKYADNLRAYYRYCRDHDITLSHAFIQPYACK 162 >A9U7F1.1 A9U7F1_PHYPA Predicted protein (Fragment) Length = 416 Score = 182 bits (461), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 89/163 (54%), Positives = 114/163 (69%) Query: 2 GWWAMAVKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKF 61 G M VKNGKEY ERI++ Q +W +GK I+ LS+H AF GLM TQA +YDMQ +++ Sbjct: 35 GAIRMPVKNGKEYIERIDQAQPEVWLRGKQIQGKLSNHAAFRGLMSTQASLYDMQVAEEY 94 Query: 62 MEQMTFQSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSL 121 + +T+ S GE GLS+L P++KEDL RRR M LW E+H G LGRSPDYMNT +M+ Sbjct: 95 AQLLTYTSPETGEPVGLSYLQPKTKEDLARRRSAMTLWGERHFGLLGRSPDYMNTAVMAY 154 Query: 122 YTAAHLLQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 A+ LL E + +Y DN++ YY YC DITLSHAF+QP ASR Sbjct: 155 AAASELLAEADAKYADNMRNYYRYCSENDITLSHAFVQPAASR 197 >A7GMD9.1 A7GMD9_BACCN 4-hydroxyphenylacetate 3-monooxygenase, oxygenase subunit Length = 490 Score = 172 bits (437), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 76/159 (47%), Positives = 108/159 (67%) Query: 6 MAVKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQM 65 M K GKEY ER+++ N+W GK ++ +S HPAF G+MKTQAE+YDMQH + E M Sbjct: 1 MPAKTGKEYIERVDQANANVWIDGKRVEGNISEHPAFKGVMKTQAELYDMQHDPEKAEYM 60 Query: 66 TFQSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAA 125 T+ S T GER G SFL P++KEDL +RR M++ W + + G +GRSPDY+N +M+ +AA Sbjct: 61 TYTSPTSGERVGTSFLQPKTKEDLAKRRKMIQEWAKYNGGMMGRSPDYINAGMMAYGSAA 120 Query: 126 HLLQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 + + + Y N+ +YYEYCR D++L+H IQP +R Sbjct: 121 EMFGKQDAFYAKNMAEYYEYCRENDLSLTHTLIQPQVNR 159 >C2WFJ6.1 C2WFJ6_BACCE 4-hydroxyphenylacetate-3-hydroxylase Length = 490 Score = 169 bits (427), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 75/159 (47%), Positives = 106/159 (66%) Query: 6 MAVKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQM 65 M K GKEY ER+++ N+W GK ++ +S HPAF G+MKTQ+E+YDMQH E M Sbjct: 1 MPAKTGKEYIERVDQADANVWVDGKRVEGKISEHPAFKGVMKTQSELYDMQHDSAKSEYM 60 Query: 66 TFQSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAA 125 T+ S T GER G SFL PR+KEDL RR M++ W + + G +GRSPDY+N +M+ +A+ Sbjct: 61 TYTSPTTGERVGTSFLQPRTKEDLQTRRKMIQEWAKYNGGMMGRSPDYINAGMMAYGSAS 120 Query: 126 HLLQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 + + + Y N+ +YYEYCR D++L+H IQP +R Sbjct: 121 EMFGKQDAFYAKNMAEYYEYCRENDLSLTHTLIQPQVNR 159 >B1HUK6.1 B1HUK6_LYSSC Pyoverdin chromophore biosynthetic protein Length = 474 Score = 156 bits (395), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 68/155 (43%), Positives = 110/155 (70%) Query: 10 NGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQMTFQS 69 G++Y ERI+ I G+++ +S HPAF G+M++QA+++D+QHQ+ ++E+MT++S Sbjct: 2 TGQQYIERIDALNTYISIDGEVVTGKVSEHPAFKGIMQSQAKLFDLQHQESYLEKMTYES 61 Query: 70 GTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAAHLLQ 129 T +R G+SFL S EDLV+RR+ W + +G +GRSPDYMNTTLM+L +A++ L+ Sbjct: 62 PTSKQRVGMSFLQVLSPEDLVKRRMAAREWALQTNGLMGRSPDYMNTTLMALASASNFLK 121 Query: 130 EYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 + + D+L+K+YEY R DI+++H FI+P +R Sbjct: 122 DKPNCFPDHLQKFYEYARENDISMTHTFIEPQVNR 156 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.134 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,096,188,286 Number of extensions: 41759139 Number of successful extensions: 91528 Number of sequences better than 1.0e-05: 266 Number of HSP's gapped: 91255 Number of HSP's successfully gapped: 266 Length of query: 190 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 62 Effective length of database: 1,854,806,283 Effective search space: 114997989546 Effective search space used: 114997989546 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)