BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0483.0010.p (935 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7A3.1 B3K7A3_9BACI O-antigen polymerase 657 0.0 B1I5B4.1 B1I5B4_DESAP O-antigen polymerase 86 2e-14 A4XML1.1 A4XML1_CALS8 O-antigen polymerase 82 2e-13 Q8RC08.1 Q8RC08_THETN TPR-repeat-containing proteins 82 4e-13 A6TJR2.1 A6TJR2_ALKMQ O-antigen polymerase 81 4e-13 >B3K7A3.1 B3K7A3_9BACI O-antigen polymerase Length = 819 Score = 657 bits (1696), Expect = 0.0, Method: Compositional matrix adjust. Identities = 346/768 (45%), Positives = 456/768 (59%), Gaps = 5/768 (0%) Query: 1 LGHPVYGKKATLSKNAERISSPLWVLIIGIILFLIWTPFQVGLFNGLRADYEKPLYISVV 60 + +PVYGKKA S+ + + WVLI GIILFL WTPFQ LFNG +EKP++ + + Sbjct: 1 MSNPVYGKKAAESRIEGKRPALFWVLISGIILFLFWTPFQTALFNGQELQFEKPIFWAAI 60 Query: 61 ICSLMLIVWAGIYYKKFKLEDQRDXXXXXXXXXXXXXXXXXFVAASHYMAINMLLIQSMY 120 + +L+L +W YYK +L+D+RD A SH +A+NM++I +Y Sbjct: 61 LATLLLFLWIAQYYKSIQLKDERDWLAVGVLLLPLTYILSLISAVSHSLAMNMVVINCIY 120 Query: 121 AALFIVGLYLLRQKQVNALIQTAIMTVAYLIVWFGLLNWLGAWNVAGEAVGWFSNTVAGS 180 A +FI+GLY+LR K N +IQ+ IM +AYLIV FGLL+W G AG V WFSNTV + Sbjct: 121 AMMFIIGLYVLRDKLGNRIIQSTIMAIAYLIVGFGLLHWFGQGKTAGAIVKWFSNTVL-N 179 Query: 181 GKYLDAVMTDSNGLRLTSVFQYANTYXXXXXXXXXXXXXXXIRSPKWYGTVIHSFMLVPV 240 G Y AVMTDSNGLRLTSVFQYANTY S KWYG HSFMLVP+ Sbjct: 180 GTYTHAVMTDSNGLRLTSVFQYANTYAAFLMAFLFAAVFFATTSRKWYGQAFHSFMLVPI 239 Query: 241 IXXXXXXXXXXXXXXXXXXXXXXXXFQKPSRQILWIIHXXXXXXXXXXXIGKVTAIGKRL 300 I F KP++Q+LWI++ VT +G L Sbjct: 240 IVSLLLTLSRSGLVLLPVVFVLLLLFLKPAKQLLWILYCGIAGVASLIISKPVTELGLDL 299 Query: 301 AAVPDASAAAEGWSWLLIACALTAAISWAVQHFAAPRLEKRLESWSTRKLANLWLPIGXX 360 ++ A +GW LL A+ + W +Q F AP+LEK L +WS +K +N+W+PI Sbjct: 300 NETFNSGGALKGWGILLGTSAIVGLLGWVIQSFIAPKLEKALSNWSAKKASNMWIPISSL 359 Query: 361 XXXXXXXWLLIGTSARSILPANIETRLENINFEQHSVLERITFYRDAFKVAKDYPVLXXX 420 +L IGT+ +++LP NI RLENINF QHSVLER TFY+DA KV DYP++ Sbjct: 360 VLGGLLIFLFIGTNLKNVLPQNISVRLENINFNQHSVLERFTFYKDAMKVLADYPIIGAG 419 Query: 421 XXXXXXLYEKYQNNPYVSRQAHNFFLQYLIEVGILGFIVFMGFILFVFYKYTKGYIKRAG 480 LYE+YQNNPY S QAHNFFLQYLIEVG++GF++FM FI +VFYKY +GYI RA Sbjct: 420 GGAWASLYEQYQNNPYTSTQAHNFFLQYLIEVGLIGFLIFMSFIAYVFYKYVRGYI-RAD 478 Query: 481 TDDYDNGFFYLIIALSILVHSILDFNMSYAFMGILVFIALGGMXXXXXXXXXXXRWN--S 538 + +N F YLI+ LSIL+HSILDFNMSY FMGILVF+ LGGM +WN Sbjct: 479 DEGRNNHFLYLILVLSILIHSILDFNMSYVFMGILVFLGLGGM-ASVMDSKPIKKWNMQD 537 Query: 539 SVLRFGYLAVTCAAALAVFIVSLRSIGSTNAFAEAKAVAQYSTSYEEIKAPLMKSLKIRP 598 S R Y AV A++ + I S+R + + A EA+ + Q STS+EEIKAPL K L IRP Sbjct: 538 STARNIYSAVVGLASIIILITSIRYVQAAEAGLEARKIVQVSTSFEEIKAPLDKDLSIRP 597 Query: 599 SHPESVILLSVLDNQVYSQTKNEQFAAESLSVLQRGLKDEPNNKQMLLQQIALYDLQGKS 658 SHPESV+LL+ L +Y Q+++E F S ++L + L+DEP+NK ML Q + Y++ + Sbjct: 598 SHPESVLLLNSLYQSLYQQSQDEGFFRLSTNLLNQVLEDEPHNKSMLNQLVKSYEMNKQP 657 Query: 659 ESAYAVYRDNLNKYIWDIDWYEPLITRASGLGVXXXXXXXXXXXXEYFQTALTTYQDVAD 718 E AY + +N K+ WDI+WYE +I + LG+ +YF A + V + Sbjct: 658 EQAYEILINNAYKFKWDINWYEQIINKGYDLGMKALGEGDTAGKEKYFNFATEAFVKVQE 717 Query: 719 GVAFLKTLPPGQLQGRPFSVTPLIALSAGKVKQITGDAETALNFIQTG 766 G+ L TLP GQ QG PF TP + L AGK+ + E A ++ G Sbjct: 718 GIKHLATLPEGQFQGNPFENTPDMILHAGKMYFMMNQPEQAAEALKLG 765 >B1I5B4.1 B1I5B4_DESAP O-antigen polymerase Length = 806 Score = 86.3 bits (212), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 45/149 (30%), Positives = 76/149 (51%) Query: 373 TSARSILPANIETRLENINFEQHSVLERITFYRDAFKVAKDYPVLXXXXXXXXXLYEKYQ 432 R++L ++ R I+ E+ S +R+T RDA ++ +DYPV LY +YQ Sbjct: 339 AGGRALLAEDVFVRAGAISGEERSFTDRMTMNRDALRIVRDYPVTGAGGGGWNALYHQYQ 398 Query: 433 NNPYVSRQAHNFFLQYLIEVGILGFIVFMGFILFVFYKYTKGYIKRAGTDDYDNGFFYLI 492 + PY + + HN+F Q +E G +GF + F Y + + A + + + + Sbjct: 399 SYPYWTTETHNYFFQTWVEAGTVGFAAVLALWGFFVYLLVRLWRYGARNGVWVSTWAAAV 458 Query: 493 IALSILVHSILDFNMSYAFMGILVFIALG 521 L + +HS DFN+S A MG+L+F +G Sbjct: 459 AVLGLGLHSFFDFNLSMAAMGLLLFAYMG 487 >A4XML1.1 A4XML1_CALS8 O-antigen polymerase Length = 726 Score = 82.0 bits (201), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 47/146 (32%), Positives = 84/146 (57%), Gaps = 5/146 (3%) Query: 378 ILPANIETRLENINFEQHSVLERITFYRDAFKVAKDYPVLXXXXXXXXXLYEKYQNNPYV 437 ++P +I ++++I+F+ + +ER+ FY+D K+ P+ LY YQ+ Y Sbjct: 346 LIPTSIVDKIKSISFKDRNFVERLVFYKDGLKIFLKSPIFGYGGGTWVSLYFMYQSYLYF 405 Query: 438 SRQAHNFFLQYLIEVGILGFIVFMGFILFVFYKYTKGYIKRAGTDDYDNGFFYLII--AL 495 + Q+HN+FLQ L++ G+ GF + M F+ +F + ++K+ D N II A+ Sbjct: 406 TTQSHNYFLQVLLDTGLFGFAILMVFLYTLFSTALRAWLKK---DKDKNILIAGIIGGAV 462 Query: 496 SILVHSILDFNMSYAFMGILVFIALG 521 + +HSILDF+ S A + +L+F +LG Sbjct: 463 QLYLHSILDFDFSLASVQVLLFASLG 488 >Q8RC08.1 Q8RC08_THETN TPR-repeat-containing proteins Length = 827 Score = 81.6 bits (200), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 47/144 (32%), Positives = 78/144 (54%), Gaps = 2/144 (1%) Query: 379 LPANIETRLENINFEQHSVLERITFYRDAFKVAKDYPVLXXXXXXXXXLYEKYQNNPYVS 438 LP +I +R+++IN S +R+ FY D K+ KDYP+ LY KYQ+ Y + Sbjct: 464 LPEDIASRVQDINLTSRSSSQRMQFYIDGLKIFKDYPIFGAGGGAWAALYFKYQSYLYWT 523 Query: 439 RQAHNFFLQYLIEVGILGFIVFMGFILFVFYKYTKGYIKRAGTDDYDNGFFYLIIAL-SI 497 Q HN+F+Q L++ GI+GFI + F+L + + G D Y +I A+ S+ Sbjct: 524 TQTHNYFMQVLLDTGIVGFIALL-FLLVTLIRSMARVYRGYGEDKYKVLIAGVITAIVSL 582 Query: 498 LVHSILDFNMSYAFMGILVFIALG 521 H+ +DF+ S + + I ++ +G Sbjct: 583 YAHAAMDFDFSLSAVSIALWALIG 606 >A6TJR2.1 A6TJR2_ALKMQ O-antigen polymerase Length = 988 Score = 81.3 bits (199), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 45/143 (31%), Positives = 76/143 (53%), Gaps = 5/143 (3%) Query: 379 LPANIETRLENINFEQHSVLERITFYRDAFKVAKDYPVLXXXXXXXXXLYEKYQNNPYVS 438 LP + +R I+ + S R +YRD+ + K++P++ +Y KYQ+ PY S Sbjct: 466 LPETLVSRFNAIDLSEGSSTTRFAYYRDSLSIFKEHPLIGAGGGAWSGIYTKYQSEPYHS 525 Query: 439 RQAHNFFLQYLIEVGILGFIVFMGFILFVFYKYTKGYIKRAGTDDYDNGFFYLIIALSIL 498 +AHN++ Q L+E G++G + G I+ + T GY G + IIAL L Sbjct: 526 TEAHNYYSQTLVETGMVGITLITGLIVLI---ATLGYYAMKGKNIVQYSIVMGIIAL--L 580 Query: 499 VHSILDFNMSYAFMGILVFIALG 521 HS +DFN SY + +++++ +G Sbjct: 581 GHSAIDFNFSYLSIALILWVLIG 603 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,686,252,864 Number of extensions: 175607163 Number of successful extensions: 433545 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 433537 Number of HSP's successfully gapped: 21 Length of query: 935 Length of database: 3,060,696,203 Length adjustment: 145 Effective length of query: 790 Effective length of database: 1,694,649,028 Effective search space: 1338772732120 Effective search space used: 1338772732120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 136 (57.0 bits)