BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0483.0005.p (240 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KCG4.1 B3KCG4_9BACI Short-chain dehydrogenase/reductase SDR 294 4e-78 B1DEY2.1 B1DEY2_9BACL Short-chain dehydrogenase/reductase SDR 183 9e-45 A9U7F9.1 A9U7F9_PHYPA Predicted protein 181 6e-44 B8I9D4.1 B8I9D4_CLOCE Short-chain dehydrogenase/reductase SDR 133 1e-29 Q1DCS2.1 Q1DCS2_MYXXD CsgA protein 128 4e-28 >B3KCG4.1 B3KCG4_9BACI Short-chain dehydrogenase/reductase SDR Length = 240 Score = 294 bits (753), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 138/240 (57%), Positives = 177/240 (73%) Query: 1 MKKVACVTGADRGLGFFLVRWLLENKYKVFAGQYFEESQALEALQAQYQDHLNLVPLDIG 60 M+ +ACVTGADRGLG L +WLLEN Y VFAG+Y E+ AL+ L Y D L L+PLDIG Sbjct: 1 METIACVTGADRGLGLSLAKWLLENGYTVFAGRYMEDWDALDRLLEDYPDQLELIPLDIG 60 Query: 61 SSESVKRAARSIAGCTGHLDIIINNAGVIDQADDATILVDMDDTAMAHIYNINTLGSLRV 120 SV++AA+ I HLD++INNAG+ ++D A I ++D MA IYN+NTLG LRV Sbjct: 61 DDASVQQAAKLIRDKAPHLDLLINNAGITKRSDLANIQGELDFADMAQIYNVNTLGPLRV 120 Query: 121 SNAXXXXXXXXXXXXXVNISSEAGSISRNQRINMYGYCMSKAALNMQSSLMHNHLRVMGG 180 S+A VNISSEAGS+SRN+R MYGYCMSK+ALNMQSS++H HL GG Sbjct: 121 SHALIELLLQGNNKLVVNISSEAGSVSRNKRKEMYGYCMSKSALNMQSSILHQHLSADGG 180 Query: 181 QVMVFHPGWLQSYMSGEKDEAAPIPPEESASKIMSIVEDHKAHMGKEPVFLDFQGEPWPW 240 QVMVF+PGWLQSYMSGE ++ A +PP+ SA +IM +V DHKA++G++P+FLD++G+ WPW Sbjct: 181 QVMVFYPGWLQSYMSGELNQEAAVPPDVSADRIMKLVMDHKAYVGEQPIFLDYEGQVWPW 240 >B1DEY2.1 B1DEY2_9BACL Short-chain dehydrogenase/reductase SDR Length = 242 Score = 183 bits (465), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 102/244 (41%), Positives = 146/244 (59%), Gaps = 8/244 (3%) Query: 1 MKKVACVTGADRGLGFFLVRWLLENKYKVFAGQYFEESQALEALQAQYQDHLNLVPLDIG 60 M + VTGADRG+G + LE+ +VFAGQY +S L+ LQ Y + L + PLD+ Sbjct: 3 MIRTLLVTGADRGVGMAIAAKWLEDGNRVFAGQY-ADSPGLQELQRLYGEQLTVFPLDVA 61 Query: 61 SSESVKRAARSIAGCTGHLDIIINNAGVIDQADDATILVDMDDTAMAHIYNINTLGSLRV 120 S SVK+A + T LD++INNA ++ + TIL ++ M ++N+N LGSLR+ Sbjct: 62 SDASVKQAYDWVRSRTDKLDVLINNAAILGDIRN-TILGELQYEEMLSVFNVNALGSLRM 120 Query: 121 SNAXXXXXXXXXXXXXVNISSEAGSISRNQRINMYGYCMSKAALNMQSSLMHNHLRVMGG 180 S A NISSEAGSI+ R Y YCMSK+A+NMQS L+HN LR GG Sbjct: 121 SQAFTPLLLQSDAKLIANISSEAGSITDCWRDAWYAYCMSKSAVNMQSKLIHNGLRPQGG 180 Query: 181 QVMVFHPGWLQSYMSGEKDEAAPIPPEESASKIMSIVED----HKAHMGKEPVFLDFQGE 236 +V+V HPGW++++M G+ DEAA + PE+SA+ I +++ A M E ++D++G Sbjct: 181 RVLVIHPGWVKTHMQGKLDEAAELTPEQSAAGITKVIQSAITRDAAEM--ECEYVDYEGR 238 Query: 237 PWPW 240 W Sbjct: 239 KLNW 242 >A9U7F9.1 A9U7F9_PHYPA Predicted protein Length = 241 Score = 181 bits (458), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 101/242 (41%), Positives = 141/242 (58%), Gaps = 7/242 (2%) Query: 3 KVACVTGADRGLGFFLVRWLLENKYKVFAGQYFEESQALEALQAQYQDHLNLVPLDIGSS 62 K A VTGADRGLGF L LLE Y VFAG+Y + +AL+ + +D L+++ LD+ Sbjct: 2 KHAFVTGADRGLGFDLTLELLERGYTVFAGRYMPDWEALDGVPEGRRDRLHVLELDVSDP 61 Query: 63 ESVKRAARSIAGCTGHLDIIINNAGVIDQADDATILVDMDDTAMAHIYNINTLGSLRVSN 122 SV++AA +++G T LD+I+NNAG I + +I D+ + +Y +N +G LR+ Sbjct: 62 ASVEKAAAAVSGKTDSLDLIVNNAG-ISGDKEGSIFGDISYEHLNRMYAVNAVGPLRIVQ 120 Query: 123 AXXXXXXXXXXXXXVNISSEAGSISRNQRINMYGYCMSKAALNMQSSLMHNHLRVMGGQV 182 A VNISSEAG I++ R YGYCMSKAALN+QS+++HN L+ GG+V Sbjct: 121 ALSGLLLKGADKLVVNISSEAGQINQAWREGWYGYCMSKAALNIQSNIVHNELKGHGGKV 180 Query: 183 MVFHPGWLQSYMSG----EKDEAAPIPPEESASKIMSIVEDHKAHMGKEPVFLDFQGEPW 238 +V HPGW++SYM G E D E+ A I +ED + K P F D+ G Sbjct: 181 LVLHPGWMKSYMGGVLGTEGDLTTAEAAEQIAGTIQRYLEDREER--KHPDFRDYAGREM 238 Query: 239 PW 240 W Sbjct: 239 SW 240 >B8I9D4.1 B8I9D4_CLOCE Short-chain dehydrogenase/reductase SDR Length = 234 Score = 133 bits (334), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 81/241 (33%), Positives = 127/241 (52%), Gaps = 8/241 (3%) Query: 1 MKKVACVTGADRGLGFFLVRWLLENKYKVFAGQYFEESQALEALQAQYQDHLNLVPLDIG 60 MKKV +TG GLG L ++ Y V+ G ++ + L+ Y + D+ Sbjct: 1 MKKV-LITGVSGGLGTCLANDFIKKNYYVY-GYDKTYNEKISVLETDYPNQFKFFNTDVS 58 Query: 61 SSESVKRAARSIAGCTGHLDIIINNAGVIDQADDATILVDMDDTAMAHIYNINTLGSLRV 120 +V+ A+ I + LDI+IN A ++ + A IL D + + +++ N+LG LRV Sbjct: 59 LDSAVEETAKKIKQNSDTLDILINAAAIL-PTNSANILEDFEISQSLEVFSTNSLGPLRV 117 Query: 121 SNAXXXXXXXXXXXXXVNISSEAGSISRN-QRINMYGYCMSKAALNMQSSLMHNHLRVMG 179 + +NISSEAGS++ + +N Y YCMSKAALNMQS ++ +L+ G Sbjct: 118 VKSLLPLIRKGEDKLIINISSEAGSLATHCNYVNRYDYCMSKAALNMQSVILQRYLKPDG 177 Query: 180 GQVMVFHPGWLQSYMSGEKDEAAPIPPEESASKIMSIVEDHKAHMGKEPVFLDFQGEPWP 239 +V+ HPGW+++ M G API P ESA+ I+++ E + H E +F D+ G Sbjct: 178 IKVLAVHPGWVRTEMGGLD---APILPSESAAGIVTLAEKY-IHKTGEGIFFDYDGNTRE 233 Query: 240 W 240 W Sbjct: 234 W 234 >Q1DCS2.1 Q1DCS2_MYXXD CsgA protein Length = 229 Score = 128 bits (322), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 83/237 (35%), Positives = 125/237 (52%), Gaps = 13/237 (5%) Query: 6 CVTGADRGLGFFLVRWLLENKYKVFAG-QYFEESQALEALQAQYQDHLNLVPLDIGSSES 64 +TGA RG+GF V+ LL V AG + E ++ LE L+ + + L + LD+G +S Sbjct: 4 VITGASRGIGFEFVQQLLLRGDTVEAGVRSPEGARRLEPLKQKAGNRLRIHALDVGDDDS 63 Query: 65 VKRAARSIAGCTGHLDIIINNAGVIDQADDATILVDMDDTAMAHIYNINTLGSLRVSNAX 124 V+ A ++ CTG +D++INNAGV + L D+D MA + IN LG LRV++A Sbjct: 64 VRAFATNV--CTGPVDVLINNAGV---SGLWCALGDVDYADMARTFTINALGPLRVTSAM 118 Query: 125 XXXXXXXXXXXXVNISSEAGSISRNQRINMYGYCMSKAALNMQSSLMHNHLRVMGGQVMV 184 +++S GS++ N Y Y MSKAALNM M LR G ++ Sbjct: 119 LPGLRQGALRRVAHVTSRMGSLAANTDGGAYAYRMSKAALNMAVRSMSTDLRPEGFVTVL 178 Query: 185 FHPGWLQSYMSGEKDEAAPIPPEESASKIMSIVED-HKAHMGKEPVFLDFQGEPWPW 240 HPGW+Q+ M G A +P +S ++ +++ + H G+ F D+QG PW Sbjct: 179 LHPGWVQTDMGGPD---ATLPAPDSVRGMLRVIDGLNPEHSGR---FFDYQGTEVPW 229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,254,365,517 Number of extensions: 43120544 Number of successful extensions: 167593 Number of sequences better than 1.0e-05: 1916 Number of HSP's gapped: 172502 Number of HSP's successfully gapped: 1917 Length of query: 240 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 108 Effective length of database: 1,817,122,223 Effective search space: 196249200084 Effective search space used: 196249200084 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)