BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0479.0016.p (309 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DF08.1 B1DF08_9BACL Inner-membrane translocator 392 e-107 A4J6I8.1 A4J6I8_DESRM Inner-membrane translocator 243 2e-62 B1I5V4.1 B1I5V4_DESAP Inner-membrane translocator 228 8e-58 B0TBU7.1 B0TBU7_HELMI Branched chain amino acid abc transporter,... 224 5e-57 Q2RH41.1 Q2RH41_MOOTA Inner-membrane translocator 219 2e-55 >B1DF08.1 B1DF08_9BACL Inner-membrane translocator Length = 309 Score = 392 bits (1006), Expect = e-107, Method: Compositional matrix adjust. Identities = 189/309 (61%), Positives = 226/309 (73%) Query: 1 MDFTTQLLIAAISXXXXXXXXXXXXXXNERAGIIQLGAEGLMLMGAVTTCIVYIRSGNXX 60 MD Q+++AAIS ER+GIIQLGAEGLMLMGAV C+ Y+ +GN Sbjct: 1 MDLFVQIIVAAISSGTPLLFATLGGILIERSGIIQLGAEGLMLMGAVIACMTYLHTGNLA 60 Query: 61 XXXXXXXXXXXXXXXXHAFLCVTLRANQTMSGLAMTLFGSGLSAYLGKSISGIPLPGTSP 120 HAF+CVTLRANQ +SGLA+TLFG+GLSAYLGKSI+G P+ G P Sbjct: 61 LTLLAACAISAVLGIVHAFMCVTLRANQIVSGLALTLFGTGLSAYLGKSITGQPVAGAVP 120 Query: 121 KLDLGWVKGIPVVGDIFGRMDGLTWFSXXXXXXXXXXIHRTSWGLHLRAVGDSPATADVM 180 K++L W++ +P++G IF +D LTWFS IHRTSWGLHLRA+GD+P TADVM Sbjct: 121 KVNLNWLEDVPLIGGIFAHLDVLTWFSFILVIVLHLYIHRTSWGLHLRAIGDNPPTADVM 180 Query: 181 GIRVQLIRYSYXXXXXXXXXXXXXDMVLAYAPTWNEGLTAGRGWIAVGLVIFARWNPLRA 240 GIRVQ RY Y D+++ Y+PTW EG+TAGRGWIAV L+IFARWNP+RA Sbjct: 181 GIRVQTFRYGYIIVGSMLIGLAGADLLMVYSPTWIEGMTAGRGWIAVALIIFARWNPIRA 240 Query: 241 LFCAYFFGALDSLGFRIQLLGSIIPPYFLKMIPYVVTILVLMYLGYRNRNKPSGTPESLG 300 LFCAYFFGALD++GFRIQL+GS IP YFLKMIPY+VTILVLM+LG+RNRNKPSG+PESLG Sbjct: 241 LFCAYFFGALDTIGFRIQLIGSEIPSYFLKMIPYIVTILVLMFLGWRNRNKPSGSPESLG 300 Query: 301 TPYIREQRF 309 TPYIREQRF Sbjct: 301 TPYIREQRF 309 >A4J6I8.1 A4J6I8_DESRM Inner-membrane translocator Length = 310 Score = 243 bits (619), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 129/281 (45%), Positives = 175/281 (62%) Query: 28 NERAGIIQLGAEGLMLMGAVTTCIVYIRSGNXXXXXXXXXXXXXXXXXXHAFLCVTLRAN 87 ER+GI+ LG EG+ML+GAV+ I++ + HAFL VTL+AN Sbjct: 30 TERSGILNLGVEGMMLVGAVSGFAFAIKTNDPWLGVLAAMLGGGAMALIHAFLTVTLQAN 89 Query: 88 QTMSGLAMTLFGSGLSAYLGKSISGIPLPGTSPKLDLGWVKGIPVVGDIFGRMDGLTWFS 147 Q +SGLA+T+FG+GLS ++GKS GIP+ + + LG++ IP +G IF D L + + Sbjct: 90 QVVSGLALTIFGTGLSGFIGKSYIGIPVVNSFKPVTLGFLSDIPFLGPIFFHHDYLVYVT 149 Query: 148 XXXXXXXXXXIHRTSWGLHLRAVGDSPATADVMGIRVQLIRYSYXXXXXXXXXXXXXDMV 207 I+RT GL+LRAVG++PA+AD +G+ V IRY Y + Sbjct: 150 YLLVPTLWFLIYRTRPGLNLRAVGENPASADSLGVSVYRIRYIYVIMGGMLAGIGGAYLS 209 Query: 208 LAYAPTWNEGLTAGRGWIAVGLVIFARWNPLRALFCAYFFGALDSLGFRIQLLGSIIPPY 267 LAYAPTW E +TAGRGWIAV LVIFA WNPLRA+F +Y FG +D+LGFR Q +G +IP + Sbjct: 210 LAYAPTWLENMTAGRGWIAVALVIFATWNPLRAMFGSYIFGGIDALGFRAQAMGVMIPSF 269 Query: 268 FLKMIPYVVTILVLMYLGYRNRNKPSGTPESLGTPYIREQR 308 FLKM+PY TI VL+ + + G+P +LG PY RE+R Sbjct: 270 FLKMLPYFFTIFVLIMVTRKAMANRVGSPGALGLPYDREER 310 >B1I5V4.1 B1I5V4_DESAP Inner-membrane translocator Length = 305 Score = 228 bits (580), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 125/280 (44%), Positives = 170/280 (60%) Query: 29 ERAGIIQLGAEGLMLMGAVTTCIVYIRSGNXXXXXXXXXXXXXXXXXXHAFLCVTLRANQ 88 ER+G++ LG EG+ML+GAV+ +V + +G+ HAFL VTL+ANQ Sbjct: 26 ERSGVLNLGVEGMMLVGAVSGFMVAVGTGDPWLGVLTAMLAGGALALVHAFLSVTLKANQ 85 Query: 89 TMSGLAMTLFGSGLSAYLGKSISGIPLPGTSPKLDLGWVKGIPVVGDIFGRMDGLTWFSX 148 +SGLA+TLFG+G SAYLG+S G PL ++ L + +P++G IF R D L S Sbjct: 86 VVSGLALTLFGTGFSAYLGRSYIGTPLDRPLCEMHLPLLSDLPLLGPIFFRHDALVLLSY 145 Query: 149 XXXXXXXXXIHRTSWGLHLRAVGDSPATADVMGIRVQLIRYSYXXXXXXXXXXXXXDMVL 208 ++RT G++LRAVG++PA AD +G+ V L RY Y + L Sbjct: 146 LLVPALWLLVYRTRAGMNLRAVGENPAAADSLGVNVSLTRYGYVIAGGMLAGIGGAYLSL 205 Query: 209 AYAPTWNEGLTAGRGWIAVGLVIFARWNPLRALFCAYFFGALDSLGFRIQLLGSIIPPYF 268 Y P W E + AGRGWIA+ LVIFA WNPL A+ AY FG +D+LGFR+Q L + PYF Sbjct: 206 VYVPAWMENMVAGRGWIAIALVIFATWNPLNAMLGAYIFGGIDALGFRLQTLDVAVSPYF 265 Query: 269 LKMIPYVVTILVLMYLGYRNRNKPSGTPESLGTPYIREQR 308 LKM+PY+ TI VL+ + +N + G P +LGTPY RE+R Sbjct: 266 LKMLPYLFTIGVLILVTGKNVRRRIGAPGALGTPYNREER 305 >B0TBU7.1 B0TBU7_HELMI Branched chain amino acid abc transporter, permease protein Length = 310 Score = 224 bits (572), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 129/281 (45%), Positives = 171/281 (60%) Query: 28 NERAGIIQLGAEGLMLMGAVTTCIVYIRSGNXXXXXXXXXXXXXXXXXXHAFLCVTLRAN 87 +ERAGI+ LG EG+ML+GAVT +V + N HAFLC+TLRAN Sbjct: 30 SERAGILNLGVEGMMLVGAVTGFLVTHSAQNEWSGLAMATVAGGLMACIHAFLCITLRAN 89 Query: 88 QTMSGLAMTLFGSGLSAYLGKSISGIPLPGTSPKLDLGWVKGIPVVGDIFGRMDGLTWFS 147 Q +SGLA+T+FGSGLS YLGKS+ G+PL T + + + IP +G+I + D L + S Sbjct: 90 QVVSGLALTIFGSGLSGYLGKSLIGVPLANTFKPVPIPVLSDIPFIGEILFKHDVLVYIS 149 Query: 148 XXXXXXXXXXIHRTSWGLHLRAVGDSPATADVMGIRVQLIRYSYXXXXXXXXXXXXXDMV 207 + RT GL LRAVG++PA AD I V RY+ + Sbjct: 150 YVLIAIIWFFLFRTKAGLELRAVGENPAAADAAAINVFATRYACVIAGGMLAGIAGAYLS 209 Query: 208 LAYAPTWNEGLTAGRGWIAVGLVIFARWNPLRALFCAYFFGALDSLGFRIQLLGSIIPPY 267 LAYAP+W E +TAGRGWIAV LVIF WNP+RA F ++FFG +D LGF +Q LG IP + Sbjct: 210 LAYAPSWLENMTAGRGWIAVALVIFGMWNPIRAFFGSFFFGTIDVLGFTMQTLGIQIPSF 269 Query: 268 FLKMIPYVVTILVLMYLGYRNRNKPSGTPESLGTPYIREQR 308 FLKM+PYV+TI+VL+ + + + +PE+LG Y RE+R Sbjct: 270 FLKMLPYVLTIVVLIVVTRETKTRRIASPEALGLSYNREER 310 >Q2RH41.1 Q2RH41_MOOTA Inner-membrane translocator Length = 311 Score = 219 bits (558), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 120/281 (42%), Positives = 170/281 (60%), Gaps = 1/281 (0%) Query: 29 ERAGIIQLGAEGLMLMGAVTTCIVYIRSGNXXXXXXXXXXXXXXXXXXHAFLCVTLRANQ 88 ERAG++ LG EG+ML+GAVT +V +++ + A L +TLRANQ Sbjct: 31 ERAGVLNLGVEGMMLVGAVTGFMVAVKTASPWLGFLAALVAAGATAAIFAVLTITLRANQ 90 Query: 89 TMSGLAMTLFGSGLSAYLGKSISGIPLPGTSPKLDLGWVKGIPVVGDIFGRMDGLTWFSX 148 ++GLA+T+FG+GLS +LGK GIPLP + + L + IP +G IF R D L + S Sbjct: 91 VVTGLALTIFGTGLSGFLGKPYVGIPLPVSFKPVALPLLSQIPFIGPIFFRHDPLVYLSY 150 Query: 149 XXXXXXXXXIHRTSWGLHLRAVGDSPATADVMGIRVQLIRYSYXXXXXXXXXXXXXDMVL 208 +RT GL+LRAVG++PA AD +G+ V +RY Y + L Sbjct: 151 ILVPALWYYFYRTRPGLNLRAVGENPAAADALGVNVFALRYIYVIIGGMLAGAGGAFLSL 210 Query: 209 AYAPTWNEGLTAGRGWIAVGLVIFARWNPLRALFCAYFFGALDSLGFRIQLLGSI-IPPY 267 AYAP+W E +TAGRGWIAV LVIFA W+P++AL +Y FG +D+L + +Q I IP + Sbjct: 211 AYAPSWLENMTAGRGWIAVALVIFAVWDPVKALLGSYLFGGVDALTYGLQAATKIAIPSF 270 Query: 268 FLKMIPYVVTILVLMYLGYRNRNKPSGTPESLGTPYIREQR 308 FLKM+PY++T++VL++ + K G P +L PY RE+R Sbjct: 271 FLKMLPYILTVIVLIFATRQTIVKHVGAPGALSIPYDREER 311 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.143 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,526,802,049 Number of extensions: 55671891 Number of successful extensions: 148766 Number of sequences better than 1.0e-05: 908 Number of HSP's gapped: 145758 Number of HSP's successfully gapped: 909 Length of query: 309 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 174 Effective length of database: 1,788,859,178 Effective search space: 311261496972 Effective search space used: 311261496972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)