BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0479.0008.p (394 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DKU5.1 B1DKU5_9BACL Amidase 347 8e-94 Q5WBJ7.1 Q5WBJ7_BACSK Putative uncharacterized protein 298 4e-79 A8VUR3.1 A8VUR3_9BACI Peptidase M22, glycoprotease 290 2e-76 A7IMN6.1 A7IMN6_XANP2 Amidase 290 2e-76 C1A771.1 C1A771_GEMAT Putative amidase 275 5e-72 >B1DKU5.1 B1DKU5_9BACL Amidase Length = 395 Score = 347 bits (891), Expect = 8e-94, Method: Compositional matrix adjust. Identities = 189/383 (49%), Positives = 232/383 (60%), Gaps = 3/383 (0%) Query: 5 NLYGAFIEPELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVV 64 + Y AF+EPELE+ G G L G +FAVKDVFA+ G+ S AGNPDWLR+H P ++ + Sbjct: 3 DTYKAFVEPELELPPVGEGPLRGLTFAVKDVFAIKGYTSGAGNPDWLRTHSPWEKTASSI 62 Query: 65 SMLLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIPXXXXXXXXXXXXXXX-D 123 S LL SGA L G THTDELMYS+ G+N HYGTPVNP A DRIP D Sbjct: 63 SKLLASGARLTGTTHTDELMYSINGQNDHYGTPVNPKAPDRIPGGSSSGSAVAVAAGAVD 122 Query: 124 FALGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQAGE 183 FALGTDTGGSVRVP+AYCG+YGFRPTH V ++GVIPLAP +DTVGW+ LL Q G+ Sbjct: 123 FALGTDTGGSVRVPAAYCGIYGFRPTHDFVSIDGVIPLAPSYDTVGWMARDISLLRQVGD 182 Query: 184 VLL-GAAAPGSRGGAMGKLHIAPEAWXXXXXXXXXXXRN-GLQLLQAGAGWSGATEIAPE 241 VLL G + +L A EAW + +QL+Q +APE Sbjct: 183 VLLPGTTDAETPRQDFRRLIFAEEAWEKAEPSCRKALGSVCIQLVQTEGMTQEWRSVAPE 242 Query: 242 GLKTWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQDHSRALRMRGE 301 GL+ W + FR QG EIW THG WI R +P+FG I G + R +R Sbjct: 243 GLEAWSHAFRTTQGREIWQTHGEWIVREKPVFGESIGARFASASTISGAEAERDGALREI 302 Query: 302 IAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTLPV 361 I RLR+LLG+D +++PT+PG AP R +E+ R M LSCIAGL+GLPQVT+P Sbjct: 303 IRKRLRELLGDDGLLIIPTIPGTAPSRTIHGPAVEERRFRTMQLSCIAGLSGLPQVTIPA 362 Query: 362 IGPAGLPLGLSVIGGHGQDLRLL 384 G P+ LSVI G QDL+LL Sbjct: 363 GEVDGAPIALSVIAGVKQDLKLL 385 >Q5WBJ7.1 Q5WBJ7_BACSK Putative uncharacterized protein Length = 388 Score = 298 bits (764), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 171/391 (43%), Positives = 217/391 (55%), Gaps = 21/391 (5%) Query: 9 AFIEPELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVVSMLL 68 AFI+ +++ G+ +G LAG +FAVKDVFAV G+ +SAGNPDWL++ P+ A S ++ LL Sbjct: 7 AFIDKDMKREGAKAGILAGKTFAVKDVFAVKGYTNSAGNPDWLKTAQPADADSPSIAKLL 66 Query: 69 QSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIP-XXXXXXXXXXXXXXXDFALG 127 +GA LRG TDELMYSL GEN HYGTPVNP DR+P DFA+G Sbjct: 67 AAGADLRGMAVTDELMYSLNGENVHYGTPVNPYGADRVPGGSSSGSAVAVAAELTDFAIG 126 Query: 128 TDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQAGEVLLG 187 TDTGGS+R+PS+YCG+YGFRP+HGAV++ GVIPLA FDTVGW+ ELL G VLLG Sbjct: 127 TDTGGSIRIPSSYCGIYGFRPSHGAVDISGVIPLAESFDTVGWMARDVELLKDIGTVLLG 186 Query: 188 AAAPGSRGGAMGKLHIAPEAWXXXXXXXXXXXRNGLQLLQAGAGWSGATEIA---PEGLK 244 G E N L+ A GA I+ + + Sbjct: 187 DQTVEEEAG---------EQLLIDEALVGIAEPNVQALVLQAADRLGAKPISRWTSDRID 237 Query: 245 TWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQD----HSRALRMRG 300 FR LQ EIW HG W++ A+P F DI Q+ + M+ Sbjct: 238 EGFETFRHLQAREIWRQHGEWVQAAKPAFAADIGARFQWASTLPQQELPLYKDKQTAMKR 297 Query: 301 EIAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTLP 360 AA + ++LPT GPAP RGG +LE R+ M L+ IAGLAG PQ+T+P Sbjct: 298 AFAAD----FAPNDIVLLPTAAGPAPKRGGSGQELEAIRAKTMKLTSIAGLAGCPQITIP 353 Query: 361 VIGPAGLPLGLSVIGGHGQDLRLLSWVREIW 391 GLP+GLS IG GQDL LLSW ++ Sbjct: 354 FASVEGLPIGLSAIGCPGQDLFLLSWANRLF 384 >A8VUR3.1 A8VUR3_9BACI Peptidase M22, glycoprotease Length = 391 Score = 290 bits (741), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 160/386 (41%), Positives = 219/386 (56%), Gaps = 4/386 (1%) Query: 7 YGAFIEPELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVVSM 66 YGAFI+ L V G L +FAVKDVF + GH ++AGNP WL+SH P+ A + + Sbjct: 6 YGAFIDESLTVEPQKGGILHRKTFAVKDVFHIEGHRNTAGNPHWLQSHAPAKATAPALVH 65 Query: 67 LLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIP-XXXXXXXXXXXXXXXDFA 125 LLQ+GATL+G T TDE+MYSL GEN HYGTPVNP K IP DFA Sbjct: 66 LLQAGATLKGTTVTDEMMYSLHGENVHYGTPVNPFDKSLIPGGSSSGSAVATGAGLRDFA 125 Query: 126 LGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQAGEVL 185 +GTDTGGSVR+P++YCG++GFRP+HG V +EGVIPLA FDTVGW T LL GEVL Sbjct: 126 IGTDTGGSVRIPASYCGLFGFRPSHGKVSLEGVIPLADSFDTVGWFTRNAALLEDVGEVL 185 Query: 186 LGAAAPGSRGGAMGKLHIAPEAWXXXXXXXXXXXRNGLQLLQAGAGWSGATEIAPEGLKT 245 +G + P + + IA +A+ + ++ + I + L Sbjct: 186 IGDSMPDAPD--FKRAVIARDAFSLLTDTQHNALMSAVRSCSSKVKSHEEQRITNDDLGD 243 Query: 246 WMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQDHSRALRMRGEIAAR 305 W+++FR +QG EIW HG W++ +P FGP I D + A R + I Sbjct: 244 WVDIFRVIQGYEIWLNHGEWVKAHEPDFGPGIRERFHMAEQIKEADVAEARRRQLMIQDA 303 Query: 306 LRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTLPVIGPA 365 ++ D+ +++PT+ G P G ++++ R M L+ IAGL GLPQVT+PV Sbjct: 304 TADIMQPDTLMIIPTIAGEPPAVGLPPEEVDKIRQRTMKLTAIAGLNGLPQVTIPVKRGG 363 Query: 366 GL-PLGLSVIGGHGQDLRLLSWVREI 390 GL PL +S++ G+D LLS+V + Sbjct: 364 GLPPLAISILAPKGRDRDLLSFVTSL 389 >A7IMN6.1 A7IMN6_XANP2 Amidase Length = 393 Score = 290 bits (741), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 175/383 (45%), Positives = 212/383 (55%), Gaps = 6/383 (1%) Query: 1 MAVHNLYGAFIE-PELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAA 59 M+ GAFI P L G+ +G LAG +FAVKDV VAG + G+PDWL +HPP+A Sbjct: 1 MSAVEALGAFIPGPRLARPGAPAGPLAGLTFAVKDVMDVAGTSTGNGHPDWLTTHPPAAR 60 Query: 60 HSAVVSMLLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIP-XXXXXXXXXXX 118 + V LL +GA+L G T DEL YSL GEN HYGTP+NP A DR+P Sbjct: 61 SAVAVDRLLDAGASLAGKTIADELCYSLTGENVHYGTPLNPAAPDRVPGGSSSGSASATA 120 Query: 119 XXXXDFALGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELL 178 DFALGTD GGSVRVPS+YCG++G RPTHG + +EGV P AP FD VGW LL Sbjct: 121 GSVVDFALGTDCGGSVRVPSSYCGLFGMRPTHGRIPLEGVAPFAPSFDCVGWFARDAGLL 180 Query: 179 LQAGEVLLGAAAPGSRGGAMGKLHIAPEAWXXXXXXXXXXXRNGLQLLQAGAGWSGATEI 238 + G VLL AAP + +L EA+ L+L Q + Sbjct: 181 ERVGRVLLADAAPAP---LLTRLLAPREAFAMLSPEAAAALAPALRLAQETIAPGAEIAL 237 Query: 239 APEGLKTWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQDHSRALRM 298 AP+GL W FR LQ AEIWA+ G WIE +P FGP + A Sbjct: 238 APDGLDAWSATFRTLQAAEIWASVGPWIEAVRPAFGPGVKERFDAARTISPSAVEVACAH 297 Query: 299 RGEIAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLE-QNRSGAMMLSCIAGLAGLPQV 357 R I ARL LL + ++PTVP PAPLRG AQ+E R AM L CIAGL GLPQV Sbjct: 298 RAAIRARLDDLLQPGTAFLMPTVPRPAPLRGMATAQVEVVTRHLAMNLLCIAGLGGLPQV 357 Query: 358 TLPVIGPAGLPLGLSVIGGHGQD 380 +LP+ G+P GLS++G G D Sbjct: 358 SLPLARLEGVPFGLSLVGPAGSD 380 >C1A771.1 C1A771_GEMAT Putative amidase Length = 404 Score = 275 bits (704), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 150/368 (40%), Positives = 203/368 (55%), Gaps = 2/368 (0%) Query: 19 GSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVVSMLLQSGATLRGAT 78 G+ G L G +F +KD F VAGH + G+PDW R+HPP+ ++ V+ LL +GA + G T Sbjct: 27 GATEGPLVGLTFGLKDNFDVAGHRTGFGSPDWFRTHPPAMQNTPVLDTLLAAGARMVGKT 86 Query: 79 HTDELMYSLGGENYHYGTPVNPLAKDRIPXXXXXXXXXXXXXX-XDFALGTDTGGSVRVP 137 HT+E+ +SL GEN HYGTP+NP A DR+P DFA+G+DTGGSVR P Sbjct: 87 HTEEMTFSLTGENAHYGTPINPAAPDRVPGGSSSGSASAVAGGLVDFAIGSDTGGSVRAP 146 Query: 138 SAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQAGEVLLGAAAPGSRGGA 197 +++CG+YG RPTHG + + G PLAP FDT GW PELL + G+VL A++ G Sbjct: 147 ASFCGIYGLRPTHGRISLAGACPLAPMFDTCGWFARDPELLRRVGQVLF-ASSGAHESGE 205 Query: 198 MGKLHIAPEAWXXXXXXXXXXXRNGLQLLQAGAGWSGATEIAPEGLKTWMNVFRELQGAE 257 G L A +A+ + + G ++P+GL W VFR LQ E Sbjct: 206 PGALLYASDAFAHTMPGVDDALMPAVSAVSGVLGALRPVTVSPDGLPAWYEVFRVLQFGE 265 Query: 258 IWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQDHSRALRMRGEIAARLRQLLGEDSCIV 317 IW THG W+ + +P FG I + R I RL LL +++ +V Sbjct: 266 IWKTHGGWVRQMRPTFGAQIAPRFEAASKVTVPEVEAMTAERVRIQQRLDALLADNAVMV 325 Query: 318 LPTVPGPAPLRGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTLPVIGPAGLPLGLSVIGGH 377 LPTVP AP RG L + R ++ L CIAGL GLPQ+++P+ G P+GLS+I Sbjct: 326 LPTVPDCAPRRGLPLPETVTVRERSLALLCIAGLGGLPQLSMPLAKVEGGPIGLSLIAAR 385 Query: 378 GQDLRLLS 385 G D LL Sbjct: 386 GNDELLLD 393 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,492,709,158 Number of extensions: 104589837 Number of successful extensions: 239234 Number of sequences better than 1.0e-05: 2966 Number of HSP's gapped: 236099 Number of HSP's successfully gapped: 3036 Length of query: 394 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 256 Effective length of database: 1,760,596,133 Effective search space: 450712610048 Effective search space used: 450712610048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)