BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0479.0007.p (528 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DKU6.1 B1DKU6_9BACL Gamma-glutamyltransferase EC=2.3.2.2 654 0.0 Q65E49.1 Q65E49_BACLD YwrD 610 e-172 A7Z9I1.1 A7Z9I1_BACA2 YwrD 606 e-171 O05218.1 YWRD_BACSU Putative gamma-glutamyltransferase ywrD EC=2... 594 e-168 A9CLW8.1 A9CLW8_BACSU Gamma-poly-glutamate hydrolase 587 e-166 >B1DKU6.1 B1DKU6_9BACL Gamma-glutamyltransferase EC=2.3.2.2 Length = 526 Score = 654 bits (1687), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/523 (60%), Positives = 379/523 (72%), Gaps = 2/523 (0%) Query: 8 PVIGTKTMVVSPHYLASAAGARILQKGGNXXXXXXXXXXXXXXXYPHMTGLGGDAFWLTY 67 PV GTK MVVSPH+LA+AAGAR++++GGN YPHMTG+GGDAF+L+Y Sbjct: 4 PVTGTKAMVVSPHHLATAAGARVMERGGNAFDAAVAVSACLAVVYPHMTGVGGDAFFLSY 63 Query: 68 SAGEGRVRAYNGSGRSGYGVCRDCYAGEEAIPRRGVRSAITVPGMADSWSAVQRGYGRLT 127 A +G +RAYN SGRSG + +A E IP RG RSAITVPGM D W AVQ YGRLT Sbjct: 64 HASDGEMRAYNASGRSGGEATIEAFADVEGIPTRGPRSAITVPGMVDGWYAVQNRYGRLT 123 Query: 128 LGEVLEPAIGYAAGGFPLSPDQYGNSVLXXXX--XXXXXXXIYLPGGAVPAAGSRFVQRQ 185 EVLE A+ YA+ GFP+SPDQ+ ++ IY+PGG V AG RFVQ + Sbjct: 124 FAEVLESAVSYASEGFPMSPDQFEHTKQHAAMLGEQTATAEIYMPGGRVIPAGERFVQSK 183 Query: 186 GGXXXXXXAEGGRDAFYKGQIAGDISRYMRASGGYLTREDFADHQGNWDEPVSTDYHGYA 245 AE GRDAFY G++A +I R+++ GG LT EDFA H G W EP+ Y GY Sbjct: 184 LAATLSTIAEKGRDAFYHGEVAAEIVRFLQTEGGLLTEEDFAAHSGEWIEPIRGSYRGYD 243 Query: 246 VYQVPPNSQGFTALLALNILEHYNFGGIAHGSYEYYHLLVEALKLSFRDRDRVLTDPAFS 305 +YQVPPNSQGFT ++AL ILE ++ + HGSYEYYH+L EALKLSFRDR+R LTDP+FS Sbjct: 244 IYQVPPNSQGFTGIMALQILEQFDLAAMGHGSYEYYHVLTEALKLSFRDRNRYLTDPSFS 303 Query: 306 RIPLEPLLDKQYASLLAASISPVKAAALSSEPVGRDTAYAAVVDEEGNGVSFIQSLYFEF 365 +PL+ LL K+YAS LAASI A ++S+PVG DTAYAAVVDE+GN VSFIQSLYFEF Sbjct: 304 AVPLDRLLSKEYASELAASIKKDAAVDINSQPVGSDTAYAAVVDEDGNAVSFIQSLYFEF 363 Query: 366 GSGVVAGNTGILLQNRGSFFSLDPGHINTLEPHKRTFHTLMPAMACLDGKPAYLWXXXXX 425 GSGVVAG+TG+LLQNRGSFFSLDP HIN LEP KRTFHTLMPAMACL+GKP +L+ Sbjct: 364 GSGVVAGDTGVLLQNRGSFFSLDPNHINRLEPRKRTFHTLMPAMACLNGKPGFLYGTQGG 423 Query: 426 XXXXXXXSLLLTRMLHYGMDPQAAVDAPRFVWGRTWGEPTQELKVERRVAPEVLSKLSEA 485 +L+LTRM+ +GM+PQ AV PRFVWGRTWG PTQELK+E R+ E KL +A Sbjct: 424 EGQPQTQTLMLTRMIDFGMNPQQAVSEPRFVWGRTWGAPTQELKLEGRIPEESAEKLEKA 483 Query: 486 GHLVRSVGDYDGIVGHAHAISIDANGYRSGGTDPRCDGAAIGW 528 GHLVR + D+DGI+GHAHAI+I +G SGG DPRCDG+AIGW Sbjct: 484 GHLVRRLPDFDGIMGHAHAIAISKDGLLSGGADPRCDGSAIGW 526 >Q65E49.1 Q65E49_BACLD YwrD Length = 527 Score = 610 bits (1572), Expect = e-172, Method: Compositional matrix adjust. Identities = 307/522 (58%), Positives = 370/522 (70%), Gaps = 5/522 (0%) Query: 10 IGTKTMVVSPHYLASAAGARILQKGGNXXXXXXXXXXXXXXXYPHMTGLGGDAFWLTYSA 69 IG+K MVVSPHYLAS AG+ I++KGGN YPHMTGLGGDAFWL Y Sbjct: 6 IGSKQMVVSPHYLASLAGSIIMEKGGNAFDAAVAVSACLAVVYPHMTGLGGDAFWLMYHN 65 Query: 70 GEGRVRAYNGSGRSGYGVCRDCYAGEEAIPRRGVRSAITVPGMADSWSAVQRGYGRLTLG 129 G G ++ YNGSGRSG RD Y GE AIP RG RSAITVPGM DSW+ + YGRL+L Sbjct: 66 GSGEIKGYNGSGRSGRKADRDQYKGERAIPVRGPRSAITVPGMVDSWAEILAAYGRLSLR 125 Query: 130 EVLEPAIGYAAGGFPLSPDQYGNSVLXXX--XXXXXXXXIYLPGGAVPAAGSRFVQRQGG 187 EVL+PAI YA GFP+SPDQ N+V IYLPGG P+ G RFVQ++ Sbjct: 126 EVLKPAIMYAEKGFPVSPDQCQNTVKNAEWLSCMPITADIYLPGGKPPSPGERFVQKELA 185 Query: 188 XXXXXXAEGGRDAFYKGQIAGDISRYMRASGGYLTREDFADHQGNWDEPVSTDYHGYAVY 247 AE GRD FYKG+IA ++ Y++ +GG L EDF H+G W P+S+ Y GYA+Y Sbjct: 186 RTLTLIAEKGRDTFYKGEIAQALAAYLQNNGGILNLEDFGLHRGEWVSPLSSTYRGYAIY 245 Query: 248 QVPPNSQGFTALLALNILEHYNFGGIAHGSYEYYHLLVEALKLSFRDRDRVLTDPAFSRI 307 Q+PPNSQGFT L+ LNILE+Y+FG I HGS+EYYHLL+EALKLSF D ++VLTDP FS+I Sbjct: 246 QMPPNSQGFTGLMTLNILENYDFGMIEHGSFEYYHLLIEALKLSFSDCNKVLTDPEFSQI 305 Query: 308 PLEPLLDKQYASLLAASISPVKAAALSSEPVGRDTAYAAVVDEEGNGVSFIQSLYFEFGS 367 PL LLDK+YA LAA I + A S P+G DT+YAA VDE+GN VSFIQSLYFEFGS Sbjct: 306 PLGRLLDKEYARELAAKIG-LPALQAESRPLGSDTSYAAAVDEDGNAVSFIQSLYFEFGS 364 Query: 368 GVVAGNTGILLQNRGSFFSLDPGHINTLEPHKRTFHTLMPAMACLDGKPAYLW--XXXXX 425 GV AG+TG++LQNRG+FFSLDP H+NTLEP KRTFHTLMPAMA GKP +L+ Sbjct: 365 GVTAGDTGVILQNRGAFFSLDPDHVNTLEPGKRTFHTLMPAMAFRGGKPKFLYGTQGQGG 424 Query: 426 XXXXXXXSLLLTRMLHYGMDPQAAVDAPRFVWGRTWGEPTQELKVERRVAPEVLSKLSEA 485 + L+TRM+ YGMDPQ AV PR+VWGRTWGE + +++E RVAPEV++KL + Sbjct: 425 EGQPQTQTALITRMVDYGMDPQTAVHEPRWVWGRTWGERVEGVRIESRVAPEVIAKLRKH 484 Query: 486 GHLVRSVGDYDGIVGHAHAISIDANGYRSGGTDPRCDGAAIG 527 GH V VG++DG++GHA I ID G+ GG+DPR DGAAIG Sbjct: 485 GHHVTVVGEFDGVMGHAGIIEIDDQGFLRGGSDPRSDGAAIG 526 >A7Z9I1.1 A7Z9I1_BACA2 YwrD Length = 525 Score = 606 bits (1562), Expect = e-171, Method: Compositional matrix adjust. Identities = 303/521 (58%), Positives = 358/521 (68%), Gaps = 3/521 (0%) Query: 9 VIGTKTMVVSPHYLASAAGARILQKGGNXXXXXXXXXXXXXXXYPHMTGLGGDAFWLTYS 68 IGTK MVVSPHYLAS AGA IL+KGGN YPHMTGLGGD+FWLTY Sbjct: 5 TIGTKQMVVSPHYLASQAGAGILEKGGNAFDAAVAVSACLAVVYPHMTGLGGDSFWLTYH 64 Query: 69 AGEGRVRAYNGSGRSGYGVCRDCYAGEEAIPRRGVRSAITVPGMADSWSAVQRGYGRLTL 128 GR++AYNGSGRSG V RD Y G EAIP RG SAITVPGMADSW AV YGRL+L Sbjct: 65 KETGRIKAYNGSGRSGKHVTRDLYRGREAIPLRGTESAITVPGMADSWDAVLSAYGRLSL 124 Query: 129 GEVLEPAIGYAAGGFPLSPDQYGNSV--LXXXXXXXXXXXIYLPGGAVPAAGSRFVQRQG 186 EVL+PA+ YAA GFP+S DQ ++ L I+ P G RFVQ+ Sbjct: 125 AEVLKPALDYAANGFPVSADQCAHTERNLALLASMPYTSAIFTRNKKAPMPGERFVQKDL 184 Query: 187 GXXXXXXAEGGRDAFYKGQIAGDISRYMRASGGYLTREDFADHQGNWDEPVSTDYHGYAV 246 AE GR AFY+G +A I+ +++ +G LT +DF H+G W PVS+DY G + Sbjct: 185 AASLEQLAEKGRSAFYEGDLAQRITSFLQNNGSRLTLDDFKAHRGEWTNPVSSDYRGCKI 244 Query: 247 YQVPPNSQGFTALLALNILEHYNFGGIAHGSYEYYHLLVEALKLSFRDRDRVLTDPAFSR 306 YQ PPNSQGFT L+ LNILE+Y+F I HGS++YYHLL+EALK SF R+ VLTDPAFS Sbjct: 245 YQAPPNSQGFTGLMTLNILENYDFSQIEHGSFDYYHLLIEALKKSFLHRNEVLTDPAFSH 304 Query: 307 IPLEPLLDKQYASLLAASISPVKAAALSSEPVGRDTAYAAVVDEEGNGVSFIQSLYFEFG 366 IPL+ LLDK+YA LA I V A A+S PVG DTAYAAV D EGN VSFIQSLYFEFG Sbjct: 305 IPLDRLLDKRYAKQLAEEIGNVAAPAVS-RPVGSDTAYAAVADAEGNAVSFIQSLYFEFG 363 Query: 367 SGVVAGNTGILLQNRGSFFSLDPGHINTLEPHKRTFHTLMPAMACLDGKPAYLWXXXXXX 426 S V AG TGILLQNRGSFFSLD H+NTLEP KRTFHTLMPAMAC +GKP L+ Sbjct: 364 SAVTAGETGILLQNRGSFFSLDESHVNTLEPGKRTFHTLMPAMACKEGKPHILYGTQGGE 423 Query: 427 XXXXXXSLLLTRMLHYGMDPQAAVDAPRFVWGRTWGEPTQELKVERRVAPEVLSKLSEAG 486 + ++TR++ Y MDPQ A++ PR+VWGRTWGE + L+VE R++PE L ++G Sbjct: 424 GQPQTQTAMITRIIDYKMDPQQAINEPRWVWGRTWGEEYEGLRVESRISPETREALRQSG 483 Query: 487 HLVRSVGDYDGIVGHAHAISIDANGYRSGGTDPRCDGAAIG 527 H V +VGD+DG++GHA AI ID G+ GGTDPR DGAA G Sbjct: 484 HRVETVGDFDGLMGHAAAIVIDEEGFLQGGTDPRGDGAAAG 524 >O05218.1 YWRD_BACSU Putative gamma-glutamyltransferase ywrD EC=2.3.2.2 Length = 525 Score = 594 bits (1531), Expect = e-168, Method: Compositional matrix adjust. Identities = 299/521 (57%), Positives = 352/521 (67%), Gaps = 3/521 (0%) Query: 9 VIGTKTMVVSPHYLASAAGARILQKGGNXXXXXXXXXXXXXXXYPHMTGLGGDAFWLTYS 68 VIGTK MVVSPHYLAS AG RIL KGGN YPHMTGLGGD+FWLT+ Sbjct: 5 VIGTKQMVVSPHYLASQAGNRILDKGGNAFDAAVAVSACLAVVYPHMTGLGGDSFWLTFH 64 Query: 69 AGEGRVRAYNGSGRSGYGVCRDCYAGEEAIPRRGVRSAITVPGMADSWSAVQRGYGRLTL 128 V+ YNGSGRSG V RD Y G+ AIP RG+ SAITVPGM DSW AV + YGRL+L Sbjct: 65 QETKAVKVYNGSGRSGKNVTRDVYKGKSAIPLRGIDSAITVPGMVDSWDAVLKEYGRLSL 124 Query: 129 GEVLEPAIGYAAGGFPLSPDQ--YGNSVLXXXXXXXXXXXIYLPGGAVPAAGSRFVQRQG 186 +VLEPA YA GFP+S DQ + + I+ G P G RFVQ++ Sbjct: 125 ADVLEPARDYAQNGFPVSADQCRHTEKNIELLASTPYTADIFTRRGKAPVPGERFVQKEL 184 Query: 187 GXXXXXXAEGGRDAFYKGQIAGDISRYMRASGGYLTREDFADHQGNWDEPVSTDYHGYAV 246 AE GR AFY+G +A I +++ +G Y+T +DF H+G W PVS+DY GY+V Sbjct: 185 ADSLNLIAEKGRSAFYEGDLAQRIVSHLQNNGSYMTIDDFKAHRGEWAAPVSSDYRGYSV 244 Query: 247 YQVPPNSQGFTALLALNILEHYNFGGIAHGSYEYYHLLVEALKLSFRDRDRVLTDPAFSR 306 YQ PPNSQGFT LL LNILE+Y+F I HGS+EYYH+LVEALK SF DRD VLTDPAF+ Sbjct: 245 YQAPPNSQGFTGLLTLNILENYDFTQIEHGSFEYYHVLVEALKKSFLDRDAVLTDPAFAD 304 Query: 307 IPLEPLLDKQYASLLAASISPVKAAALSSEPVGRDTAYAAVVDEEGNGVSFIQSLYFEFG 366 IPLE LLDK+YA LA I + A S PVG DTAYAAV+D +GN VSFIQSLYFEFG Sbjct: 305 IPLERLLDKRYAKQLAEEIGYLAIPA-ESRPVGSDTAYAAVIDADGNAVSFIQSLYFEFG 363 Query: 367 SGVVAGNTGILLQNRGSFFSLDPGHINTLEPHKRTFHTLMPAMACLDGKPAYLWXXXXXX 426 S V AG+TGILLQNRGSFFSLD H+NTLEP KRTFHTLMPAM C GKP L+ Sbjct: 364 SAVTAGDTGILLQNRGSFFSLDENHVNTLEPRKRTFHTLMPAMVCKGGKPKILYGTQGGE 423 Query: 427 XXXXXXSLLLTRMLHYGMDPQAAVDAPRFVWGRTWGEPTQELKVERRVAPEVLSKLSEAG 486 + ++TRML YGM PQ A+ PR+VWGRTWGE + L+VE R + + KL ++G Sbjct: 424 GQPQTQTAIITRMLDYGMHPQQAISEPRWVWGRTWGEEYEGLRVEGRFTDKTIQKLKDSG 483 Query: 487 HLVRSVGDYDGIVGHAHAISIDANGYRSGGTDPRCDGAAIG 527 HLV VGDYD ++G A AI +D G+ GG DPR DGAA+G Sbjct: 484 HLVEVVGDYDPLMGQAAAIKVDEEGFLQGGADPRGDGAAVG 524 >A9CLW8.1 A9CLW8_BACSU Gamma-poly-glutamate hydrolase Length = 525 Score = 587 bits (1514), Expect = e-166, Method: Compositional matrix adjust. Identities = 295/521 (56%), Positives = 349/521 (66%), Gaps = 3/521 (0%) Query: 9 VIGTKTMVVSPHYLASAAGARILQKGGNXXXXXXXXXXXXXXXYPHMTGLGGDAFWLTYS 68 VIGTK MVVSPHYLAS AG RIL KGGN YPHMTGLGGD+FWLT+ Sbjct: 5 VIGTKQMVVSPHYLASQAGNRILDKGGNAFDAAVAVSACLAVVYPHMTGLGGDSFWLTFH 64 Query: 69 AGEGRVRAYNGSGRSGYGVCRDCYAGEEAIPRRGVRSAITVPGMADSWSAVQRGYGRLTL 128 V+ YNGSGRSG V RD Y G+ AIP RG+ SAITVPGM DSW AV + YGRL+L Sbjct: 65 QETKAVKVYNGSGRSGKNVTRDVYKGKSAIPLRGIDSAITVPGMVDSWDAVLKEYGRLSL 124 Query: 129 GEVLEPAIGYAAGGFPLSPDQ--YGNSVLXXXXXXXXXXXIYLPGGAVPAAGSRFVQRQG 186 +VLEPA YA GFP+S DQ + + I+ G P G RF+Q++ Sbjct: 125 ADVLEPARDYAQNGFPVSADQCRHTEKNIELLASTPYTADIFTRRGKAPVPGERFLQKEL 184 Query: 187 GXXXXXXAEGGRDAFYKGQIAGDISRYMRASGGYLTREDFADHQGNWDEPVSTDYHGYAV 246 AE GR AFY+G +A I +++ +G Y+T +DF H+G W PVS+DY GY+V Sbjct: 185 ADSLNVIAEKGRSAFYEGDLAQRIVSHLQNNGSYMTIDDFKAHRGEWAAPVSSDYRGYSV 244 Query: 247 YQVPPNSQGFTALLALNILEHYNFGGIAHGSYEYYHLLVEALKLSFRDRDRVLTDPAFSR 306 YQ PPNSQGFT LL LNILE+Y+F I HGS+EYYH+LVEALK SF DR+ LTDPAF+ Sbjct: 245 YQAPPNSQGFTGLLTLNILENYDFTQIEHGSFEYYHVLVEALKKSFVDRNAFLTDPAFAD 304 Query: 307 IPLEPLLDKQYASLLAASISPVKAAALSSEPVGRDTAYAAVVDEEGNGVSFIQSLYFEFG 366 IPLE LLDK YA LA I + A S PVG DTAYAAV+D +GN VSFIQSLYFEFG Sbjct: 305 IPLERLLDKNYAKRLAGEIGYLAEPA-ESRPVGSDTAYAAVIDADGNAVSFIQSLYFEFG 363 Query: 367 SGVVAGNTGILLQNRGSFFSLDPGHINTLEPHKRTFHTLMPAMACLDGKPAYLWXXXXXX 426 S V AG+TGILLQNRGSFFSLD H+NTLEP KRTFHTLMPAM C GKP L+ Sbjct: 364 SAVTAGDTGILLQNRGSFFSLDENHVNTLEPRKRTFHTLMPAMVCKGGKPKILYGTQGGE 423 Query: 427 XXXXXXSLLLTRMLHYGMDPQAAVDAPRFVWGRTWGEPTQELKVERRVAPEVLSKLSEAG 486 + ++TRML YGM PQ A+ PR+VWGRTWGE + L+VE R + + KL ++G Sbjct: 424 GQPQTQTAIITRMLDYGMHPQQAISEPRWVWGRTWGEEYEGLRVEGRFTDKTIQKLKDSG 483 Query: 487 HLVRSVGDYDGIVGHAHAISIDANGYRSGGTDPRCDGAAIG 527 HLV VGDYD ++G A AI +D G+ G DPR DGAA+G Sbjct: 484 HLVEVVGDYDPLMGQAAAIKVDEEGFLQAGADPRGDGAAVG 524 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,252,250,702 Number of extensions: 140586019 Number of successful extensions: 283032 Number of sequences better than 1.0e-05: 1770 Number of HSP's gapped: 275572 Number of HSP's successfully gapped: 1892 Length of query: 528 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 387 Effective length of database: 1,732,333,088 Effective search space: 670412905056 Effective search space used: 670412905056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)