BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0068.p (374 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6M2.1 B3K6M2_9BACI Major facilitator superfamily MFS_1 223 3e-56 C0ZL32.1 C0ZL32_BREBN Conserved hypothetical membrane protein 172 4e-41 Q9KEG8.1 Q9KEG8_BACHD BH0884 protein 163 3e-38 B7GFW5.1 B7GFW5_ANOFW Permease of the major facilitator superfamily 159 6e-37 O34929.1 YFKF_BACSU Uncharacterized MFS-type transporter yfkF 153 3e-35 >B3K6M2.1 B3K6M2_9BACI Major facilitator superfamily MFS_1 Length = 289 Score = 223 bits (567), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 127/227 (55%), Positives = 162/227 (71%), Gaps = 1/227 (0%) Query: 39 AALYVGSFAMTLVAERILSRIGFKQLIAAXXXXXXXXXXXXXXXXXVKAWFLLRLLVGAG 98 AALYVGSFAMTLVAER+L +GFK+LI + V WF+LR+LVG G Sbjct: 54 AALYVGSFAMTLVAERLLGWVGFKRLIVSGLVLVMLSLVAFPLIPSVAVWFVLRVLVGIG 113 Query: 99 DSAINYAAQLWVLLMTPAAHRGRNLSLYGMSYGLGFSLGPLGISLLRFGQXXXXXXXXXX 158 DSA++YAAQLWVL+M+ +RGR++S YGMSYGLGFS+GPLGI LL++G+ Sbjct: 114 DSALHYAAQLWVLMMSTVKNRGRSISFYGMSYGLGFSIGPLGIPLLKYGEAVPFLILAVL 173 Query: 159 XXXXXXXXXXXXXDSRPDKAEHGEGQ-ARRFVRSYSLAWYALIPALLYGYMEASLNSNFP 217 + +P+K+E GE Q A R+++SY LAW+ALIPA LYGYMEA +NSNFP Sbjct: 174 FLFILLVVLFKLPNLKPEKSEQGEQQPAGRYLKSYRLAWFALIPAFLYGYMEAGINSNFP 233 Query: 218 VYGLRIGFSANEIAALLPFAGIGGLLLQMPLGIWSDRYGRKKILIVS 264 VYGLR GF+ EI+ LLPF GIGGLLLQ+PLGIWSD++GRK++L ++ Sbjct: 234 VYGLRSGFTLGEISTLLPFVGIGGLLLQLPLGIWSDKFGRKRVLTIA 280 >C0ZL32.1 C0ZL32_BREBN Conserved hypothetical membrane protein Length = 394 Score = 172 bits (437), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 105/335 (31%), Positives = 158/335 (47%), Gaps = 1/335 (0%) Query: 39 AALYVGSFAMTLVAERILSRIGFKQLIAAXXXXXXXXXXXXXXXXXVKAWFLLRLLVGAG 98 AALY+G + E L RIG++ I + WF+LRLL+G G Sbjct: 48 AALYIGMVMVNPFLEIPLRRIGYRATILCGLLLVTIATALIPMFSHLAVWFVLRLLMGVG 107 Query: 99 DSAINYAAQLWVLLMTPAAHRGRNLSLYGMSYGLGFSLGPLGISLLRFGQXXXXXXXXXX 158 DS ++YA+Q+WV + +RGR+LS+YG++YG+GFS+GPLG+S+L G Sbjct: 108 DSGLHYASQMWVTKIAAPENRGRDLSIYGLAYGVGFSVGPLGLSVLHLG-IWVPFGGLLV 166 Query: 159 XXXXXXXXXXXXXDSRPDKAEHGEGQARRFVRSYSLAWYALIPALLYGYMEASLNSNFPV 218 + P + E E + ++ LAW ALIP+ LYG+ME SLN +FPV Sbjct: 167 LYLIAFLLLSRIKNEFPAQIEQTEKKQNKYAFVLRLAWLALIPSFLYGFMETSLNGSFPV 226 Query: 219 YGLRIGFSANEIAALLPFAGIGGLLLQMPLGIWSDRYGRKKILIVSXXXXXXXXXXXXXX 278 Y LR G S ++ +LP +G ++LQMPLG SDR GRK++++V Sbjct: 227 YALRTGLSVEWVSLILPSFVVGSIILQMPLGALSDRVGRKQVMLVCALVGGLAFFLFPFA 286 Query: 279 EDRFLWTXXXXXXXXXXXXXXXXXXXXYAADILPRNLLPAANVVSSFHYSIGSITXXXXX 338 + ++ADILP +++P A +++ ++ + SI Sbjct: 287 GENVWLMMLLLAIAGAAVGSFYSLGLAFSADILPASMVPTAGIIAGVNFGVASILAPNVN 346 Query: 339 XXXXXXXXXXXXXXXMGILYILFGLAGLLFSPRQT 373 MG L I+F A L F + T Sbjct: 347 GLLMDVWEPWTIFWLMGSLLIVFAAACLFFKQKAT 381 >Q9KEG8.1 Q9KEG8_BACHD BH0884 protein Length = 401 Score = 163 bits (412), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 94/296 (31%), Positives = 139/296 (46%), Gaps = 2/296 (0%) Query: 39 AALYVGSFAMTLVAERILSRIGFKQLIAAXXXXXXXXXXXXXXXXXVKAWFLLRLLVGAG 98 AALY+G + E+ L R G+K +I WF+LR+++G Sbjct: 54 AALYIGILLASPFIEKPLRRYGYKPIITIGLMAMIVSLLVLPLWQAFWFWFVLRMIIGIA 113 Query: 99 DSAINYAAQLWVLLMTPAAHRGRNLSLYGMSYGLGFSLGPLGISLLRFGQXXXXXXXXXX 158 D+ +++A Q+W+ +P RGRN+S YG ++GLGF LGP LL+ + Sbjct: 114 DNMVHFATQVWITTTSPMKKRGRNISFYGFAFGLGFGLGPFMTRLLQINEFLPFILSAIT 173 Query: 159 XXXXXXXXXXXXXDSRPDKAEHGE--GQARRFVRSYSLAWYALIPALLYGYMEASLNSNF 216 + E G G R+ L W+AL+PA YGY+E+SL+ NF Sbjct: 174 SFAAWLLILRLRNEYPAQDVETGSQSGTWTRYKAVVKLGWFALLPAFCYGYLESSLHGNF 233 Query: 217 PVYGLRIGFSANEIAALLPFAGIGGLLLQMPLGIWSDRYGRKKILIVSXXXXXXXXXXXX 276 PVYGLR G + +++ LLP +GGL+ QMPLG SDR GRK +L++ Sbjct: 234 PVYGLRSGLTVEQVSILLPAFVVGGLITQMPLGFLSDRMGRKPLLLMVLFFGGATFMSMV 293 Query: 277 XXEDRFLWTXXXXXXXXXXXXXXXXXXXXYAADILPRNLLPAANVVSSFHYSIGSI 332 E F+ Y AD+LP +LLP NV+ + + +GS+ Sbjct: 294 YLEHTFIGLFLSFALAGAFVGSLYSLGVMYMADLLPPHLLPTGNVMMAVSFGLGSM 349 >B7GFW5.1 B7GFW5_ANOFW Permease of the major facilitator superfamily Length = 390 Score = 159 bits (401), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 96/295 (32%), Positives = 140/295 (47%), Gaps = 2/295 (0%) Query: 39 AALYVGSFAMTLVAERILSRIGFKQLIAAXXXXXXXXXXXXXXXXXVKAWFLLRLLVGAG 98 A+Y+G ++ ER L R GFK+L+ + WF+LRLL+G G Sbjct: 46 TAMYIGVLIVSPFLERPLRRYGFKRLMMLGGAIVIASLAMFPISSSLSFWFMLRLLIGIG 105 Query: 99 DSAINYAAQLWVLLMTPAAHRGRNLSLYGMSYGLGFSLGPLGISLLRFGQXXXXXXXXXX 158 D +++ +Q W+ +P RGRN+SLYG+S+G+GF +GPL + L+ + Sbjct: 106 DHILHFGSQTWITYASPIHRRGRNISLYGLSFGVGFMIGPLLVPLV-YVHEALPFIVSSF 164 Query: 159 XXXXXXXXXXXXXDSRPDKAEHGEGQARRFVRSYSLAWYALIPALLYGYMEASLNSNFPV 218 + P E + RFVR++ AW AL+ YG++EASL+S FPV Sbjct: 165 LSLCGWMLLFFLPHTYPQATEAEKIGGLRFVRAWKQAWPALLLPFGYGFLEASLHSMFPV 224 Query: 219 YGLRIGFSANEIAALLPFAGIGGLLLQMPLGIWSDRYGRKKILIVSXXXXXXXXXXXXXX 278 Y LR G +A +LP +GG+ Q+PLG SDR+ RK I++VS Sbjct: 225 YALRNGLHVETVAIMLPAFALGGIAFQLPLGTLSDRFSRKTIIVVSLTIGAICFMIAQLF 284 Query: 279 EDRFLWTXXXXXXXXXXXXXXXXXXXXYAADILPRNLLPAANVVSSFHYSIGSIT 333 L T Y D+LP++LLP N++ YSIGSI Sbjct: 285 SSPVLLT-MCFFIAGMFVGSLFSLGMAYMTDLLPKSLLPVGNILCGMLYSIGSIC 338 >O34929.1 YFKF_BACSU Uncharacterized MFS-type transporter yfkF Length = 391 Score = 153 bits (386), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 91/297 (30%), Positives = 139/297 (46%), Gaps = 5/297 (1%) Query: 39 AALYVGSFAMTLVAERILSRIGFKQLIAAXXXXXXXXXXXXXXXXXVKAWFLLRLLVGAG 98 LY+G + E L ++GFK LI V WFLLRL +G G Sbjct: 46 TGLYIGVLLASPFMEAPLRKLGFKPLIVMGGSIVILSLFGFIWLQSVWVWFLLRLFIGIG 105 Query: 99 DSAINYAAQLWVLLMTPAAHRGRNLSLYGMSYGLGFSLGPLGISLLRFGQXXXXXXXXXX 158 D ++++ Q WV M+ +RGRNLS+YG+S+GLGF+ GP + L++ Sbjct: 106 DHMLHFSTQTWVTSMSSKQNRGRNLSIYGLSFGLGFAAGPFMVPLVKL-SPSLPFIVSGC 164 Query: 159 XXXXXXXXXXXXXDSRPDKAEH---GEGQARRFVRSYSLAWYALIPALLYGYMEASLNSN 215 ++ P+ + H + RRF ++ W A +P YG++E +LN + Sbjct: 165 ISLFAWLFVFFLQNAYPETSPHETKSDNSFRRFYQAMLFGWVAFMPTFGYGFLETALNGS 224 Query: 216 FPVYGLRIGFSANEIAALLPFAGIGGLLLQMPLGIWSDRYGRKKILIVSXXXXXXXXXXX 275 FPVY LR+G S + +A +LP IG ++ Q PLGI SD+YGR+ +L+V Sbjct: 225 FPVYALRLGISVDAVAIILPAFAIGSIIFQFPLGILSDKYGRRNVLLVILLTGALCFFIA 284 Query: 276 XXXEDRFLWTXXXXXXXXXXXXXXXXXXXXYAADILPRNLLPAANVVSSFHYSIGSI 332 ++ Y D+LP +LLPA N++ +S+GSI Sbjct: 285 GVFPSPYV-IGGCFFIAGMAVGSTFTLGISYMTDLLPPHLLPAGNLLCGITFSLGSI 340 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.143 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,628,521,837 Number of extensions: 49857547 Number of successful extensions: 168345 Number of sequences better than 1.0e-05: 136 Number of HSP's gapped: 168186 Number of HSP's successfully gapped: 156 Length of query: 374 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 237 Effective length of database: 1,770,017,148 Effective search space: 419494064076 Effective search space used: 419494064076 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)