BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0066.p (250 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6M4.1 B3K6M4_9BACI Haloacid dehalogenase domain protein hydro... 326 1e-87 B1DLZ3.1 B1DLZ3_9BACL Haloacid dehalogenase domain protein hydro... 261 3e-68 C4E1F5.1 C4E1F5_STRRS Predicted HAD superfamily hydrolase, COG1011 106 2e-21 Q67NF7.1 Q67NF7_SYMTH Putative uncharacterized protein 104 9e-21 A8M147.1 A8M147_SALAI Haloacid dehalogenase domain protein hydro... 103 1e-20 >B3K6M4.1 B3K6M4_9BACI Haloacid dehalogenase domain protein hydrolase Length = 245 Score = 326 bits (836), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 153/240 (63%), Positives = 188/240 (78%) Query: 1 MKYLTQQVIFDLDDTLVHCNKYFDLILGQYFELMSEWFGEYGGTTAEFRSKQVEIDVQTV 60 M +Q +IFD+DDTL+HCNKYFDLILG YFE M+EWF YG TT E R+KQ+EIDV + Sbjct: 1 MSKTSQHIIFDMDDTLIHCNKYFDLILGDYFEWMTEWFQPYGVTTQEVRAKQMEIDVAGI 60 Query: 61 STSGLASDNFPKSLIATYRYFCIKYNRPADPYQEQQLMKLGMSVYDQEVEAYPGMVETLD 120 G AS NFP+SLI TY +F + R P +E+ L +LG+SVY+QE+EAYPGMVETL+ Sbjct: 61 DKLGFASANFPQSLIDTYHFFGRIHGRDTSPKEEELLYQLGLSVYEQEIEAYPGMVETLE 120 Query: 121 TLKQDGHALYLYTGGDDTIQQRKIEQMKLDVYFDDRIYIRQHKNVEALENILNTHSFDRK 180 L++DGH L+LYTGG+ IQ+RKIEQMKL YF DRIYIRQHKN+ ALE IL SF R Sbjct: 121 KLREDGHCLHLYTGGETRIQKRKIEQMKLADYFKDRIYIRQHKNITALEEILGMGSFVRS 180 Query: 181 NTWMIGNSLRTDVLPALTAGINSIYLKQQNEWLYNLIELQREMQQSVQTITSISEVPPVI 240 NTWMIGNSLRTDV+PALTAGINSIY+KQQ EW YNL+EL++E + T+++++EVP VI Sbjct: 181 NTWMIGNSLRTDVVPALTAGINSIYIKQQREWQYNLVELKQEPHNVLYTVSALTEVPEVI 240 >B1DLZ3.1 B1DLZ3_9BACL Haloacid dehalogenase domain protein hydrolase Length = 247 Score = 261 bits (668), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 122/238 (51%), Positives = 168/238 (70%), Gaps = 1/238 (0%) Query: 4 LTQQVIFDLDDTLVHCNKYFDLILGQYFELMSEWFGEYGGTTAE-FRSKQVEIDVQTVST 62 + Q + FDLDDTL+HCNKYF ++ Q+ + M+ WF Y G +A+ R+KQ EID+ V+ Sbjct: 1 MKQNLFFDLDDTLIHCNKYFYFVIEQFVDAMTTWFAGYEGVSADAVRNKQTEIDIAGVAV 60 Query: 63 SGLASDNFPKSLIATYRYFCIKYNRPADPYQEQQLMKLGMSVYDQEVEAYPGMVETLDTL 122 G S++FP+S I TY Y+C R ++ L KLG+SVY+ E E YP M TLD L Sbjct: 61 LGFKSEHFPQSFIDTYHYYCDLTGRKKSKTEQDFLWKLGLSVYEHETEPYPNMEATLDKL 120 Query: 123 KQDGHALYLYTGGDDTIQQRKIEQMKLDVYFDDRIYIRQHKNVEALENILNTHSFDRKNT 182 +GH L+LYTGG+ TIQ+RKIEQMKL+ YFD R+YIR+HKN EALE IL +R T Sbjct: 121 AGEGHTLHLYTGGEITIQRRKIEQMKLERYFDTRVYIRRHKNSEALEQILAEGGHNRHLT 180 Query: 183 WMIGNSLRTDVLPALTAGINSIYLKQQNEWLYNLIELQREMQQSVQTITSISEVPPVI 240 WMIGNS+RTDV+PALTAGI++I+++ ++EW YN+I ++ E + + T+ ++ EVPP I Sbjct: 181 WMIGNSIRTDVVPALTAGIHAIHMRAESEWQYNVINIEVEPKGAFLTLNNLLEVPPAI 238 >C4E1F5.1 C4E1F5_STRRS Predicted HAD superfamily hydrolase, COG1011 Length = 230 Score = 106 bits (265), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 71/221 (32%), Positives = 108/221 (48%), Gaps = 13/221 (5%) Query: 1 MKYLTQQVIFDLDDTLVHCNKYFDLILGQYFELMSEWFGEYGGTTAEFRSKQVEIDVQTV 60 M++ Q +IFD DDTL N F+ ++ + + W E R+ +I Sbjct: 1 MRFEGQVLIFDADDTLWENNVLFERVIDDF----THWLEHPTLDRTEIRAILDDIQAANA 56 Query: 61 STSGLASDNFPKSLIATYRYFCIKY--NRPADPYQEQQLMKLGMSVYDQEVEAYPGMVET 118 T G S F +SL C++ RPA + + L +L M++ + VE PG+ ET Sbjct: 57 VTHGYGSKMFLRSLDE-----CLERLRERPATAAERRHLDELVMALVEHRVELVPGVAET 111 Query: 119 LDTLKQDGHALYLYTGGDDTIQQRKIEQMKLDVYFDDRIYIRQHKNVEALENILNTHSFD 178 L L H L L T GD QQRK++ L +F D I+I KN++ + H Sbjct: 112 LADLGS-RHDLLLLTKGDVDEQQRKLDVSGLAPHFRD-IHIVAEKNLDTYRWLTREHGLA 169 Query: 179 RKNTWMIGNSLRTDVLPALTAGINSIYLKQQNEWLYNLIEL 219 +WMIGNS ++D+LPA AG+N++++ N W+ EL Sbjct: 170 PATSWMIGNSPKSDILPARQAGMNAVFIPNDNTWVLEQSEL 210 >Q67NF7.1 Q67NF7_SYMTH Putative uncharacterized protein Length = 239 Score = 104 bits (259), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 64/237 (27%), Positives = 115/237 (48%), Gaps = 8/237 (3%) Query: 4 LTQQVIFDLDDTLVHCNKYFDLILGQYFELMSEWFGEYGGTTAEFRSKQVEIDVQTVSTS 63 T D DDT+ +YF + Q L++ G G E + + E+DV Sbjct: 3 FTGHAFVDWDDTIAENIRYFREVEAQISLLIA---GRTGFDRDEVQRRGAELDVAVARQM 59 Query: 64 GLASDNFPKSLIATYRYFCIKYNRPADPYQEQQLMKLGMSVYDQEVEAYPGMVETLDTLK 123 GL ++ + + YR F + RP DP E +L +L Y+ E PG ETL L Sbjct: 60 GLVRESLAVAWLTCYREFAGRAGRPVDPVVEAELRRLCAIPYETRQELLPGAAETLRWLH 119 Query: 124 QDGHALYLYTGGDDTIQQRKIEQMKLDVYFDDRIYIRQHKNVEALENILNTHSFDRKNTW 183 + G + ++T G+D +Q+RKI++ L + + + + K E L + + D + + Sbjct: 120 EAGFEVTIWTAGEDAVQRRKIQESGLTRWIHRQTVVPE-KTAERLRDAIGDR--DPGHCF 176 Query: 184 MIGNSLRTDVLPALTAGINSIYLKQQNEWLYNLIELQREMQQSVQTITSISEVPPVI 240 ++GNS +D+ PALT G+ + ++ + W Y+ +++ E + +T I E+P ++ Sbjct: 177 VVGNSRHSDIRPALTLGVAAYHVDVET-WAYDHLDVDEE-DPNYHRLTHIGELPRLL 231 >A8M147.1 A8M147_SALAI Haloacid dehalogenase domain protein hydrolase Length = 231 Score = 103 bits (257), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 64/208 (30%), Positives = 103/208 (49%), Gaps = 9/208 (4%) Query: 6 QQVIFDLDDTLVHCNKYFDLILGQYFELMSEWFGEYGGTTAEFRSKQVEIDVQTVSTSGL 65 Q +IFD DDTL N F+ ++ + E W A+ R+ +I+ T G Sbjct: 7 QTLIFDADDTLWENNVLFENVIESFIE----WLAHPTLDRAQVRTILDDIEAANAVTHGY 62 Query: 66 ASDNFPKSLIATYRYFCIKYNRPADPYQEQQLMKLGMSVYDQEVEAYPGMVETLDTLKQD 125 S F +SL + RP P + Q++ +L ++ D++VE PG+ ETL L Q Sbjct: 63 GSAMFLRSLHDCFERL---RERPGSPQERQEIEELARALLDRKVELIPGVTETLAEL-QS 118 Query: 126 GHALYLYTGGDDTIQQRKIEQMKLDVYFDDRIYIRQHKNVEALENILNTHSFDRKNTWMI 185 H L L+T G Q+ K E L+ F ++I + K ++ + D TWMI Sbjct: 119 RHRLLLFTKGHPEEQRHKFELSGLERLFAG-VHIVEEKKTADYLRLIADNELDPGVTWMI 177 Query: 186 GNSLRTDVLPALTAGINSIYLKQQNEWL 213 GNS ++D++PA AG+N++++ N W+ Sbjct: 178 GNSPKSDIIPARQAGMNAVFIPNDNTWV 205 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,455,352,670 Number of extensions: 57854600 Number of successful extensions: 145637 Number of sequences better than 1.0e-05: 119 Number of HSP's gapped: 145703 Number of HSP's successfully gapped: 119 Length of query: 250 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 118 Effective length of database: 1,817,122,223 Effective search space: 214420422314 Effective search space used: 214420422314 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)