BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0063.p (263 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZG67.1 C0ZG67_BREBN Probable DNA topoisomerase III EC=5.99.1.2 452 e-125 B3K6M5.1 B3K6M5_9BACI DNA topoisomerase EC=5.99.1.2 398 e-109 B1DLZ4.1 B1DLZ4_9BACL DNA topoisomerase EC=5.99.1.2 348 3e-94 B0TFR0.1 B0TFR0_HELMI DNA topoisomerase EC=5.99.1.2 223 1e-56 C1TJI1.1 C1TJI1_9FIRM DNA topoisomerase III 221 5e-56 >C0ZG67.1 C0ZG67_BREBN Probable DNA topoisomerase III EC=5.99.1.2 Length = 752 Score = 452 bits (1162), Expect = e-125, Method: Compositional matrix adjust. Identities = 214/258 (82%), Positives = 237/258 (91%), Gaps = 2/258 (0%) Query: 1 LKTLIIAEKPDMGRNIAAAIEPKAKNYRSYLEGEQYIITWAIGHLIGLAEPEAYDSKYKK 60 +K LIIAEKPDMGRNIAAAI+PKAKN+R+YLEGE+Y+ITWAIGHLI LAEPEAYD KYKK Sbjct: 1 MKILIIAEKPDMGRNIAAAIDPKAKNHRTYLEGEKYMITWAIGHLIELAEPEAYDQKYKK 60 Query: 61 WNINDLPIIXXXXEFKLVPNARTLDQLKVIGELAKRSDLLVNSCDAGREGQHIFSLIQRH 120 WNINDLPII FKLVPN +T+DQLK+IG+LAKRS+LLVN+CDAGREGQHIFSLIQRH Sbjct: 61 WNINDLPIIPE--PFKLVPNRKTIDQLKIIGQLAKRSNLLVNACDAGREGQHIFSLIQRH 118 Query: 121 LKLSQPVKRLWISDLTPETIRKGFQELKDGSEYENLTKAAKARSEADWLIGMNGSRAFTT 180 LKL+QPVKRLWISDLTPETIRKGF E+KD + YENLT+AAKARSEADWLIGMNGSRAFTT Sbjct: 119 LKLTQPVKRLWISDLTPETIRKGFAEMKDAAAYENLTRAAKARSEADWLIGMNGSRAFTT 178 Query: 181 KHNVLLSVGRVQTPVLALIYDRQKTIEAFSSLKFFEVEGHFTQNEVVYKGMWQGERMTDG 240 KHNVLLSVGRVQTPVLALIYDRQK IEAFSS+ FF+VEGHF+Q E +YKG+WQG+R+TD Sbjct: 179 KHNVLLSVGRVQTPVLALIYDRQKQIEAFSSVTFFQVEGHFSQGERMYKGLWQGDRLTDP 238 Query: 241 AKAEALAAKVKGKPARIA 258 AKAE LA KVKGK RIA Sbjct: 239 AKAEMLARKVKGKQGRIA 256 >B3K6M5.1 B3K6M5_9BACI DNA topoisomerase EC=5.99.1.2 Length = 762 Score = 398 bits (1022), Expect = e-109, Method: Compositional matrix adjust. Identities = 186/257 (72%), Positives = 218/257 (84%), Gaps = 2/257 (0%) Query: 1 LKTLIIAEKPDMGRNIAAAIEPKAKNYRSYLEGEQYIITWAIGHLIGLAEPEAYDSKYKK 60 +KTL+IAEKPDMGRNIAA IEP+AKN R+YLEGEQYIITWAIGHL+GLAEP+AYD KYK+ Sbjct: 1 MKTLVIAEKPDMGRNIAAVIEPRAKNNRTYLEGEQYIITWAIGHLVGLAEPDAYDPKYKR 60 Query: 61 WNINDLPIIXXXXEFKLVPNARTLDQLKVIGELAKRSDLLVNSCDAGREGQHIFSLIQRH 120 WN+ DLPII +FK++PN +T DQLKVIGE+AKR +VN+CDAGREGQ+IF+LIQ+ Sbjct: 61 WNLRDLPIIPE--QFKIIPNPKTKDQLKVIGEVAKRCSHIVNACDAGREGQYIFALIQQQ 118 Query: 121 LKLSQPVKRLWISDLTPETIRKGFQELKDGSEYENLTKAAKARSEADWLIGMNGSRAFTT 180 LKL QPVKRLWISDLTPE+I +GF ELKDG E+ENLT+AA+ARSEADWLIGMN SRAFTT Sbjct: 119 LKLKQPVKRLWISDLTPESIGRGFAELKDGVEFENLTQAARARSEADWLIGMNASRAFTT 178 Query: 181 KHNVLLSVGRVQTPVLALIYDRQKTIEAFSSLKFFEVEGHFTQNEVVYKGMWQGERMTDG 240 +H LLSVGRVQTPVLALIYDRQK IEAF S F++V+ F Q+++ Y G WQGER+T Sbjct: 179 RHQTLLSVGRVQTPVLALIYDRQKEIEAFESQTFYDVKALFKQDQLEYTGTWQGERLTSQ 238 Query: 241 AKAEALAAKVKGKPARI 257 KAE +AAKV GK I Sbjct: 239 EKAETIAAKVNGKNGTI 255 >B1DLZ4.1 B1DLZ4_9BACL DNA topoisomerase EC=5.99.1.2 Length = 770 Score = 348 bits (892), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 176/257 (68%), Positives = 205/257 (79%), Gaps = 2/257 (0%) Query: 1 LKTLIIAEKPDMGRNIAAAIEPKAKNYRSYLEGEQYIITWAIGHLIGLAEPEAYDSKYKK 60 +KTLIIAEKPDMGR IAA +EPKA N R+YLEG+++IITWAIGHL+ LAEP+ YD++YK+ Sbjct: 1 MKTLIIAEKPDMGRTIAAVVEPKAANKRTYLEGDRFIITWAIGHLVTLAEPDQYDARYKR 60 Query: 61 WNINDLPIIXXXXEFKLVPNARTLDQLKVIGELAKRSDLLVNSCDAGREGQHIFSLIQRH 120 WN DLPII +FKL PNART DQLK IGELAKR LVN+CDAGREGQ IF LI+++ Sbjct: 61 WNDQDLPIIPD--KFKLWPNARTKDQLKTIGELAKRCGRLVNACDAGREGQLIFHLIRQY 118 Query: 121 LKLSQPVKRLWISDLTPETIRKGFQELKDGSEYENLTKAAKARSEADWLIGMNGSRAFTT 180 LKL QP RLWISDLTPETIRKGF L+ +Y LT+AA+ARSEADWLIGMN SRAFT Sbjct: 119 LKLPQPTDRLWISDLTPETIRKGFDSLRSDEDYAPLTRAARARSEADWLIGMNASRAFTI 178 Query: 181 KHNVLLSVGRVQTPVLALIYDRQKTIEAFSSLKFFEVEGHFTQNEVVYKGMWQGERMTDG 240 +H LLSVGRVQTPVLAL+YDR K I AF S ++ V+ F QN Y G+WQG+R+TD Sbjct: 179 RHKALLSVGRVQTPVLALLYDRHKEITAFKSDMYYVVKALFDQNGFQYSGIWQGDRITDK 238 Query: 241 AKAEALAAKVKGKPARI 257 AKAEALAAK +GKP I Sbjct: 239 AKAEALAAKTQGKPGFI 255 >B0TFR0.1 B0TFR0_HELMI DNA topoisomerase EC=5.99.1.2 Length = 781 Score = 223 bits (568), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 121/259 (46%), Positives = 162/259 (62%), Gaps = 9/259 (3%) Query: 1 LKTLIIAEKPDMGRNIAAAIEPKAKNYRSYLEGEQYIITWAIGHLIGLAEPEAYDSKYKK 60 +KTLIIAEKP IA A+ + R YLE + ITWA+GHL+ LAEPE Y S K+ Sbjct: 1 MKTLIIAEKPSAAEAIAEAVAGTYQKKRGYLESPDFYITWAVGHLVQLAEPEDYRSSLKR 60 Query: 61 WNINDLPIIXXXXEFKLVPNARTLDQLKVIGELAKRSDLLVNSCDAGREGQHIFSLIQRH 120 W DLPII +F+L P +T QLK I LAK+S L+N+CDA REG+ IF I + Sbjct: 61 WRFGDLPIIP---DFQLRPVKKTEGQLKTIAGLAKKSRALINACDAAREGELIFRYIVAY 117 Query: 121 LKLSQ-PVKRLWISDLTPETIRKGFQELKDGSEYENLTKAAKARSEADWLIGMNGSRAFT 179 L+L + P RLW + LT E IR +LK Y+NL K+A+ RSE DWLIG+N +RAFT Sbjct: 118 LRLEEMPAWRLWTASLTAEAIRAAMADLKPLDRYDNLFKSARCRSEGDWLIGINATRAFT 177 Query: 180 TKHNVLLSVGRVQTPVLALIYDRQKTIEAFSSLKFFEVEGHFTQNEVVYKGMW-----QG 234 T+ LL++GRVQTPVLA+I + ++ +AF ++EV F Y+G+W + Sbjct: 178 TRFGELLTLGRVQTPVLAMICENRRRRDAFVPETYWEVWARFRGEGCDYRGLWLREKGEA 237 Query: 235 ERMTDGAKAEALAAKVKGK 253 R+ + KAEA+AA V G+ Sbjct: 238 HRLNEKGKAEAIAAAVDGR 256 >C1TJI1.1 C1TJI1_9FIRM DNA topoisomerase III Length = 724 Score = 221 bits (563), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 113/235 (48%), Positives = 157/235 (66%), Gaps = 6/235 (2%) Query: 1 LKTLIIAEKPDMGRNIAAAIEPKAKNYRSYLEGEQYIITWAIGHLIGLAEPEAYDSKYKK 60 +K L++AEKP + R+++ + K YLE + YIITWAIGHL+ LAEP+ YD+ KK Sbjct: 8 MKQLVLAEKPSVARDLSNVLGNFNKG-NGYLESDAYIITWAIGHLVTLAEPDDYDAALKK 66 Query: 61 WNINDLPIIXXXXEFKLVPNARTLDQLKVIGELAKRSDL--LVNSCDAGREGQHIFSLIQ 118 W +N LPI+ EFKL P + TL Q ++ L R+D+ ++ + D+GREG+ IF I Sbjct: 67 WFLNTLPILPR--EFKLKPVSATLKQFNIVKSLLHRNDVGDIICATDSGREGELIFRYIY 124 Query: 119 RHLKLSQPVKRLWISDLTPETIRKGFQELKDGSEYENLTKAAKARSEADWLIGMNGSRAF 178 + +P KRLW+S+ TP +RKGF L+ G++ + L AA+ARS+ADWLIG+N +RAF Sbjct: 125 KLTDCQKPFKRLWLSETTPAAVRKGFDNLRPGNDLDRLAFAAEARSQADWLIGINATRAF 184 Query: 179 TTKHNVLLSVGRVQTPVLALIYDRQKTIEAFSSLKFFEVEGHFT-QNEVVYKGMW 232 T KHN LLSVGRVQTP LALI +R++ I AF K++E+ FT +N Y G W Sbjct: 185 TVKHNQLLSVGRVQTPTLALIVNREQEIRAFVPEKYWELFALFTKENGQTYIGKW 239 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,513,029,655 Number of extensions: 58207218 Number of successful extensions: 133757 Number of sequences better than 1.0e-05: 2141 Number of HSP's gapped: 130585 Number of HSP's successfully gapped: 2141 Length of query: 263 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 130 Effective length of database: 1,807,701,208 Effective search space: 235001157040 Effective search space used: 235001157040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)