BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0061.p (488 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA29.1 B3KA29_9BACI Prolyl-tRNA synthetase EC=6.1.1.15 577 e-162 C0ZF57.1 SYP_BREBN Prolyl-tRNA synthetase EC=6.1.1.15 531 e-149 C5D9C3.1 C5D9C3_9BACI Prolyl-tRNA synthetase 528 e-148 Q5L0J4.1 SYP_GEOKA Prolyl-tRNA synthetase EC=6.1.1.15 523 e-146 C3J437.1 C3J437_9BACI Prolyl-tRNA synthetase 521 e-146 >B3KA29.1 B3KA29_9BACI Prolyl-tRNA synthetase EC=6.1.1.15 Length = 575 Score = 577 bits (1486), Expect = e-162, Method: Compositional matrix adjust. Identities = 289/484 (59%), Positives = 356/484 (73%), Gaps = 15/484 (3%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 MRQS LL TT+R+ P+EAE ++HQ +LR GYIRQ AAGIY+YLPLG +VLRKIE I+R+E Sbjct: 1 MRQSQLLMTTMRDVPSEAEALSHQWMLRGGYIRQTAAGIYSYLPLGWRVLRKIETIIRQE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 MDAAG QELLMP++QPAELWKES RY+VYG EL++L DRHDREFVLGPTHEEVITS++R Sbjct: 61 MDAAGGQELLMPAVQPAELWKESGRYDVYGAELIRLYDRHDREFVLGPTHEEVITSIVRQ 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 EISSYRKLP+ +YQI +K+RDERRPR GL+RGREFLMKDAYSFD WEGLD +Y MY A Sbjct: 121 EISSYRKLPVNLYQIQSKYRDERRPRFGLLRGREFLMKDAYSFDRDWEGLDASYRNMYDA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEVH 243 Y+RIF RCGL++RAV ADAGAIGGQGETHEFMA++ IGEDTV CS C YAANLE+AE Sbjct: 181 YERIFSRCGLMFRAVEADAGAIGGQGETHEFMALADIGEDTVVSCSDCRYAANLERAESA 240 Query: 244 TEAE-AVQRN---VSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAV 299 E A R+ A E+ TPG+++I +L LQ+ +Q+MKTLIY+ G+ AV Sbjct: 241 APGEPAAMRDSVKTGGSSAMERIFTPGVKSIQELTDYLQVGAEQLMKTLIYRVDGELVAV 300 Query: 300 LVRGDHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAA 359 LVRGD E NE KV++Y+GA +A +E V+ A G+ GPV + ++VD VAA Sbjct: 301 LVRGDRETNETKVKSYLGAEAAEMASAEEVKQATGAPVGFAGPVGLR--LKIMVDHEVAA 358 Query: 360 MTSGITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHI 419 M GITGA D HL+ V PG DF+LE+V DFR EGE CP CG L F +GIE+GH+ Sbjct: 359 MAEGITGANLEDTHLKGVRPGLDFSLEYVGDFRNVTEGEPCPRCG-APLQFHRGIELGHV 417 Query: 420 FKLGTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLVXXXXLQWLSRTTMGRGLSGLRH 479 FKLGTKYS+ + LDA+GR K +IMGCYGIG+SR++ Q +S + Sbjct: 418 FKLGTKYSDAMNGKYLDADGREKPLIMGCYGIGVSRVMAAIAEQRISDERL--------V 469 Query: 480 WPLT 483 WP++ Sbjct: 470 WPMS 473 >C0ZF57.1 SYP_BREBN Prolyl-tRNA synthetase EC=6.1.1.15 Length = 581 Score = 531 bits (1369), Expect = e-149, Method: Compositional matrix adjust. Identities = 263/455 (57%), Positives = 330/455 (72%), Gaps = 5/455 (1%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 M+QS +L TLRE P++AE +H+LLLRAG RQLA+GIYTYLPL + L KI+AIVREE Sbjct: 1 MKQSQMLIPTLREVPSDAEIASHKLLLRAGMARQLASGIYTYLPLALRSLHKIQAIVREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 M+ AG QELLMP+MQPAELW ++ R++VYG EL++L DRHDR F LGPTHEEVITSL+R Sbjct: 61 MNNAGGQELLMPAMQPAELWHQTGRWDVYGPELVRLRDRHDRPFALGPTHEEVITSLVRD 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 EI+SY+KLPI +YQI TKFRDE RPR GL+R REF+MKDAYSFDT +GLD + MY A Sbjct: 121 EINSYKKLPINLYQIQTKFRDEVRPRFGLIRCREFIMKDAYSFDTSQDGLDRNFQAMYDA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEVH 243 Y IF R GL +RAV ADAGAIGG+G T+EFMA+ IGEDT+A D+AANLE+AEV Sbjct: 181 YTNIFTRVGLNFRAVEADAGAIGGKG-TYEFMALCDIGEDTIAYSEEGDFAANLEKAEVV 239 Query: 244 TEAEAVQRNVSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVLVRG 303 + A + + PA EK HTPG++TIDQL Q LQI Q++K+LIY+ K VLVRG Sbjct: 240 YKPSA--KPATEAPAREKLHTPGIKTIDQLVQALQIEAKQIIKSLIYRVDDKLVMVLVRG 297 Query: 304 DHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPV-XXXXPVTLLVDRAVAAMTS 362 DHE+NEVK++N A+ + LA + + +G+VGPV V ++ D V + Sbjct: 298 DHEINEVKLKNLYDATIVGLASEADIVSVTGAPAGFVGPVGIDPEKVDIIADNFVQDVYD 357 Query: 363 GITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIFKL 422 G+ GA E D+HL VV GRDF + AD R EG+ CP G GV+ F +G+E+GH+FKL Sbjct: 358 GVVGANETDYHLTHVVAGRDFTVSKYADLRNITEGDECPRTG-GVIKFARGVEVGHVFKL 416 Query: 423 GTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLV 457 GTKYS +GA+ LD NGRS+ MIMGCYGIG+SR + Sbjct: 417 GTKYSTAMGATYLDENGRSQPMIMGCYGIGVSRTI 451 >C5D9C3.1 C5D9C3_9BACI Prolyl-tRNA synthetase Length = 567 Score = 528 bits (1360), Expect = e-148, Method: Compositional matrix adjust. Identities = 254/461 (55%), Positives = 337/461 (73%), Gaps = 5/461 (1%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 MRQS TLRE PA+AE +HQLLLRAG+IRQ A+G+YT+LPLG++VL+K+EAI+REE Sbjct: 1 MRQSQSFIPTLREVPADAEVKSHQLLLRAGFIRQSASGVYTFLPLGQRVLQKVEAIIREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 M+ AGA ELLMP++QPAELW++S R+ YG ELM+L DRH+R+F LGPTHEE+IT+L+R Sbjct: 61 MNRAGAIELLMPALQPAELWQQSGRWYSYGPELMRLKDRHERDFALGPTHEEIITALVRD 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 E+ +Y++LP+T+YQI KFRDE+RPR GL+RGREF+MKDAYSF T E LD+TY++MY+A Sbjct: 121 EVKTYKRLPLTLYQIQVKFRDEKRPRFGLLRGREFIMKDAYSFHTSQESLDETYNKMYEA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEVH 243 Y IF RCGL +RAV AD+GAIGG+ +THEFM +S IGEDT+A A DYAAN+E A V Sbjct: 181 YSNIFRRCGLNFRAVIADSGAIGGK-DTHEFMVLSEIGEDTIAYSDASDYAANIEMAPVI 239 Query: 244 TEAEAVQRNVSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVLVRG 303 T E + L EK HTPG +TI+++ LQ+ P++ +K+L+++ + VLVRG Sbjct: 240 TTYEKSDEPLRKL---EKVHTPGQKTIEEVASYLQVTPEKCIKSLLFKVDDRYVLVLVRG 296 Query: 304 DHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAMTSG 363 DHE N+VKV+N AS + LA E + A E G +GP+ VT++ D AV A+ +G Sbjct: 297 DHEANDVKVKNLFDASVVELATPEETKQAMNCEVGSLGPIGVSESVTIVADHAVKAIVNG 356 Query: 364 ITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIFKLG 423 + GA E +H V P RDF + AD R +EG+ P GKG + F +GIE+GH+FKLG Sbjct: 357 VCGANEEGYHYIGVNPERDFTISEYADLRFIQEGDPSP-DGKGTIRFARGIEVGHVFKLG 415 Query: 424 TKYSEKLGASVLDANGRSKLMIMGCYGIGISRLVXXXXLQW 464 T+YS+ + A+ LD NGRS+ MIMGCYGIG+SRLV Q+ Sbjct: 416 TRYSKAMNATYLDENGRSQTMIMGCYGIGVSRLVAAIAEQF 456 >Q5L0J4.1 SYP_GEOKA Prolyl-tRNA synthetase EC=6.1.1.15 Length = 567 Score = 523 bits (1346), Expect = e-146, Method: Compositional matrix adjust. Identities = 255/463 (55%), Positives = 336/463 (72%), Gaps = 9/463 (1%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 MRQS TLRE PA+AE +HQLLLRAG+IRQ A+G+YT+LPLG++VL+K+EAI+REE Sbjct: 1 MRQSQAFIPTLREVPADAEVKSHQLLLRAGFIRQSASGVYTFLPLGQRVLQKVEAIIREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 M+ GA EL MP++QPAELW++S R+ YG ELM+L DRH+R+F LGPTHEE+IT+++R Sbjct: 61 MNRIGAMELFMPALQPAELWQQSGRWYSYGPELMRLKDRHERDFALGPTHEEMITAIVRD 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 E+ +Y++LP+ +YQI TKFRDE+RPR GL+RGREF+MKDAYSF T E LD+TY+ MY+A Sbjct: 121 EVKTYKRLPLVLYQIQTKFRDEKRPRFGLLRGREFMMKDAYSFHTSKESLDETYNNMYEA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEVH 243 Y IF RCGL +RAV AD+GAIGG+ +THEFM +S IGEDT+A A DYAAN+E A V Sbjct: 181 YANIFRRCGLNFRAVIADSGAIGGK-DTHEFMVLSDIGEDTIAYSDASDYAANIEMAPVV 239 Query: 244 TEAEAVQRNVSNLPAAE--KFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVLV 301 E S+ P AE K TPG +TI ++ LQI+P++ +K+L++ G+ VLV Sbjct: 240 ATYEK-----SDEPPAELKKVATPGQKTIAEVASHLQISPERCIKSLLFNVDGRYVLVLV 294 Query: 302 RGDHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAMT 361 RGDHE NEVKV+N + A+ + LA E + G +GP+ VT++ D AVAA+T Sbjct: 295 RGDHEANEVKVKNVLDATVVELATPEETERVMNAPIGSLGPIGVSEDVTVIADHAVAAIT 354 Query: 362 SGITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIFK 421 +G+ GA E +H V PGRDFA+ AD R +EG+ P GKG + F +GIE+GH+FK Sbjct: 355 NGVCGANEEGYHYIGVNPGRDFAVSQYADLRFVKEGDPSP-DGKGTIRFARGIEVGHVFK 413 Query: 422 LGTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLVXXXXLQW 464 LGTKYSE + A LD NG+++ MIMGCYGIG+SRLV Q+ Sbjct: 414 LGTKYSEAMNAVYLDENGQTQTMIMGCYGIGVSRLVAAIAEQF 456 >C3J437.1 C3J437_9BACI Prolyl-tRNA synthetase Length = 567 Score = 521 bits (1341), Expect = e-146, Method: Compositional matrix adjust. Identities = 254/463 (54%), Positives = 335/463 (72%), Gaps = 9/463 (1%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 MRQS TLRE PA+AE +HQLLLRAG+IRQ A+G+YT+LPLG++VL+K+EAI+REE Sbjct: 1 MRQSQAFIPTLREVPADAEVKSHQLLLRAGFIRQSASGVYTFLPLGQRVLQKVEAIIREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 M+ GA EL MP++QPAELW++S R+ YG ELM+L DRH+R+F LGPTHEE+IT+++R Sbjct: 61 MNRIGAMELFMPALQPAELWQQSGRWYSYGPELMRLKDRHERDFALGPTHEEMITAIVRD 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 E+ +Y++LP+ +YQI TKFRDE+RPR GL+RGREF+MKDAYSF T E LD+TY+ MY+A Sbjct: 121 EVKTYKRLPLVLYQIQTKFRDEKRPRFGLLRGREFMMKDAYSFHTSKESLDETYNNMYEA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEVH 243 Y IF RCGL +RAV AD+GAIGG+ +THEFM +S IGEDT+A A DYAAN+E A V Sbjct: 181 YANIFRRCGLNFRAVIADSGAIGGK-DTHEFMVLSDIGEDTIAYSDASDYAANIEMAPVV 239 Query: 244 TEAEAVQRNVSNLPAAE--KFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVLV 301 E S+ P AE K TPG +TI ++ LQI+P++ +K+L++ G+ VLV Sbjct: 240 ATYEK-----SDEPPAELKKVATPGQKTIAEVASHLQISPERCIKSLLFNVDGRYVLVLV 294 Query: 302 RGDHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAMT 361 RGDHE NEVKV+N + A+ + LA E + G +GP+ VT++ D AVAA+ Sbjct: 295 RGDHEANEVKVKNVLDATVVELATPEETERVMNAPIGSLGPIGVSEDVTVIADHAVAAIV 354 Query: 362 SGITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIFK 421 +G+ GA E +H V PGRDFA+ AD R +EG+ P GKG + F +GIE+GH+FK Sbjct: 355 NGVCGANEEGYHYIGVNPGRDFAVSQYADLRFVKEGDPSP-DGKGTIRFARGIEVGHVFK 413 Query: 422 LGTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLVXXXXLQW 464 LGTKYSE + A LD NG+++ MIMGCYGIG+SRLV Q+ Sbjct: 414 LGTKYSEAMNAVYLDENGQTQTMIMGCYGIGVSRLVAAIAEQF 456 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,755,309,241 Number of extensions: 113231192 Number of successful extensions: 274769 Number of sequences better than 1.0e-05: 2109 Number of HSP's gapped: 270242 Number of HSP's successfully gapped: 2293 Length of query: 488 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 348 Effective length of database: 1,741,754,103 Effective search space: 606130427844 Effective search space used: 606130427844 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)