BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0058.p (360 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6M6.1 B3K6M6_9BACI Glutamate synthase, NADH/NADPH, small subunit 481 e-134 B1DLZ7.1 B1DLZ7_9BACL Glutamate synthase, NADH/NADPH, small subunit 430 e-118 A4J8A5.1 A4J8A5_DESRM Glutamate synthase (NADH) small subunit EC... 362 3e-98 B7ASD5.1 B7ASD5_9BACE Putative uncharacterized protein 306 2e-81 C4Z1P2.1 C4Z1P2_9FIRM Glutamate synthase (NADH) small chain 302 4e-80 >B3K6M6.1 B3K6M6_9BACI Glutamate synthase, NADH/NADPH, small subunit Length = 495 Score = 481 bits (1238), Expect = e-134, Method: Compositional matrix adjust. Identities = 233/324 (71%), Positives = 258/324 (79%), Gaps = 2/324 (0%) Query: 35 MSTPTGFMEYKRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGIDM 94 MSTPTGFMEY+RQLP DR PA+RVKDWEEFH HL EDEL+TQGARCMDCGTPYCHTGIDM Sbjct: 1 MSTPTGFMEYQRQLPADRSPAERVKDWEEFHKHLEEDELQTQGARCMDCGTPYCHTGIDM 60 Query: 95 SGGTSGCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXXXXXX--XXXXXXXXXX 152 GGTSGCP+HNLIPEWNNLVYRGLW+EALERL Sbjct: 61 VGGTSGCPIHNLIPEWNNLVYRGLWREALERLHKTNNFPEFTGRVCPAPCEGSCTVGLIG 120 Query: 153 XXXXIKTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVTV 212 IK+IE AIIDKGFEEGWVVP PPEKRTG+ VA+V H+VTV Sbjct: 121 EPVTIKSIEQAIIDKGFEEGWVVPEPPEKRTGRRVAVVGSGPAGLAAAAQLNKAGHSVTV 180 Query: 213 FERSDRIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYDA 272 +ER+DRIGGLLTYGIP+MKLDK +VQRRVDLLAAEGV+FVVNTEIGKDIPA+QL+++YDA Sbjct: 181 YERADRIGGLLTYGIPSMKLDKGIVQRRVDLLAAEGVEFVVNTEIGKDIPAKQLMEDYDA 240 Query: 273 VVLCGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKLEDGNYVSAAGKDVIVLGG 332 VVLCGGATK R FN+EGS+L G+ YAMDYLNGTIKS L+S LEDGNY+SA KDVIV+GG Sbjct: 241 VVLCGGATKPREFNIEGSDLKGIHYAMDYLNGTIKSLLDSGLEDGNYLSAKDKDVIVIGG 300 Query: 333 GDTGSDCVATALRHGCSSITQFGT 356 GDTGSDCVATALRHGCSS+TQFGT Sbjct: 301 GDTGSDCVATALRHGCSSVTQFGT 324 >B1DLZ7.1 B1DLZ7_9BACL Glutamate synthase, NADH/NADPH, small subunit Length = 496 Score = 430 bits (1105), Expect = e-118, Method: Compositional matrix adjust. Identities = 203/324 (62%), Positives = 244/324 (75%), Gaps = 2/324 (0%) Query: 35 MSTPTGFMEYKRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGIDM 94 MSTPTGFM+Y+R+LP DRDP +R+KDW EFH HL++++LRTQGARCMDCGTPYCHTG+++ Sbjct: 1 MSTPTGFMQYQRELPADRDPIERIKDWNEFHKHLSDEQLRTQGARCMDCGTPYCHTGMEL 60 Query: 95 SGGTSGCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXXXXXX--XXXXXXXXXX 152 +G SGCPV+NLIPEWNNL+YRGLW+EAL+RL Sbjct: 61 AGSASGCPVNNLIPEWNNLIYRGLWREALDRLHKTNNFPEFTGRVCPAPCEGSCTVGLIG 120 Query: 153 XXXXIKTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVTV 212 IKTIE AII++GFEEGWVV PP+ RTGK VA+V H VTV Sbjct: 121 EPVTIKTIENAIIERGFEEGWVVAQPPKVRTGKKVAVVGSGPAGLAAAAQLNKAGHKVTV 180 Query: 213 FERSDRIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYDA 272 +ER+DR+GGLLTYGIPTMKL+K VV RRV+L+ EG++FV NTEIGKDI +L+ E+DA Sbjct: 181 YERADRLGGLLTYGIPTMKLEKHVVDRRVNLMREEGIEFVTNTEIGKDISRDELLKEFDA 240 Query: 273 VVLCGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKLEDGNYVSAAGKDVIVLGG 332 V++CGGATKAR +EG ELNG+ AMDYLNGTIKSYL+S LEDGNY+SA KDVIV+GG Sbjct: 241 VIMCGGATKARDVEMEGRELNGIHLAMDYLNGTIKSYLDSNLEDGNYISAKDKDVIVIGG 300 Query: 333 GDTGSDCVATALRHGCSSITQFGT 356 GDTG+DCVATALRHGC S+TQFGT Sbjct: 301 GDTGTDCVATALRHGCKSVTQFGT 324 >A4J8A5.1 A4J8A5_DESRM Glutamate synthase (NADH) small subunit EC=1.4.1.14 Length = 490 Score = 362 bits (929), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 172/322 (53%), Positives = 216/322 (67%), Gaps = 2/322 (0%) Query: 35 MSTPTGFMEYKRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGIDM 94 M PTGFMEY R++ +RDP +R+ DW+EF+ L E+ L+ Q ARCM CGTP+CH+G+ + Sbjct: 1 MGKPTGFMEYGREMAANRDPRERINDWQEFYQKLPEEGLKKQAARCMACGTPFCHSGLMI 60 Query: 95 SGGTSGCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXXX--XXXXXXXXXXXXX 152 G SGCP HN+ PEWN+LVYRGLWKEA RL Sbjct: 61 KGMASGCPNHNVPPEWNDLVYRGLWKEAYLRLIKTNNFPEFTGRVCPALCEGACTAGLIG 120 Query: 153 XXXXIKTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVTV 212 +K+IE AIID+ F EGWV+P PP RTGK VA++ H VTV Sbjct: 121 SPVTVKSIECAIIDRAFAEGWVLPQPPATRTGKRVAVIGSGPAGLACAAELNKSGHWVTV 180 Query: 213 FERSDRIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYDA 272 FER+DRIGGLL YGIP MKLDK+ VQRRV+LLAAEG+KF+ TE+GKD P +L+ +DA Sbjct: 181 FERADRIGGLLMYGIPNMKLDKKAVQRRVELLAAEGIKFITKTEVGKDYPTDELLKNFDA 240 Query: 273 VVLCGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKLEDGNYVSAAGKDVIVLGG 332 VVLCGGATK R VEG L GV +A+++L+ KS L+S+ +DGN++SA GKDV+V+GG Sbjct: 241 VVLCGGATKPRDLPVEGRNLQGVHFAVEFLSINTKSLLDSQHKDGNFISAQGKDVLVIGG 300 Query: 333 GDTGSDCVATALRHGCSSITQF 354 GDTG+DCVATALRHGC S+ Q Sbjct: 301 GDTGTDCVATALRHGCKSVVQL 322 >B7ASD5.1 B7ASD5_9BACE Putative uncharacterized protein Length = 494 Score = 306 bits (784), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 145/328 (44%), Positives = 203/328 (61%), Gaps = 2/328 (0%) Query: 35 MSTPTGFMEYKRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGIDM 94 M TGFMEY+R+ P +R+K+++EFH L+ ++ RTQ ARCMDCG P+C G+ + Sbjct: 1 MGKATGFMEYEREESAAIKPKERIKNFDEFHTPLSLEKQRTQAARCMDCGVPFCQAGMMI 60 Query: 95 SGGTSGCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXXXXXXXXXXXXXXXXXX 154 G SGCP++NLIPEWN+L+Y G W++A RL Sbjct: 61 KGMASGCPLNNLIPEWNDLLYNGNWQQAYNRLHKTSNFPEFTSRVCPALCEKACTCGLNG 120 Query: 155 XXI--KTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVTV 212 + K E AII+K +EEGW P PP+ RT K +A+V H VTV Sbjct: 121 EAVCTKENEKAIIEKAYEEGWAAPKPPKVRTDKRIAVVGSGPSGLAVADQLNRRGHLVTV 180 Query: 213 FERSDRIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYDA 272 +ERSDR+GGLL YGIP MKL+K ++ R+++++ AEGV F+ + IG+++ A++L++EYD+ Sbjct: 181 YERSDRVGGLLRYGIPNMKLEKHIIDRKIEVMKAEGVTFITDANIGENVKAKKLLEEYDS 240 Query: 273 VVLCGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKLEDGNYVSAAGKDVIVLGG 332 VVL GA+ R G + NG+ +A+D+L T KS L+S L DG Y+SA GK+VIV+GG Sbjct: 241 VVLACGASNPRDIKAPGRDANGIYFAVDFLKSTTKSLLDSGLTDGKYISAKGKNVIVIGG 300 Query: 333 GDTGSDCVATALRHGCSSITQFGTXXXP 360 GDTG+DCV T++RHGC SI Q P Sbjct: 301 GDTGNDCVGTSIRHGCKSIMQLEMMPKP 328 >C4Z1P2.1 C4Z1P2_9FIRM Glutamate synthase (NADH) small chain Length = 494 Score = 302 bits (773), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 142/322 (44%), Positives = 202/322 (62%), Gaps = 2/322 (0%) Query: 35 MSTPTGFMEYKRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGIDM 94 M PTGF++Y+R P +R+K++ EFH L+EDE R Q +RCMDCG P+C +G+ + Sbjct: 1 MGKPTGFLDYERVEATAVSPKERIKNFNEFHTPLSEDEQRKQSSRCMDCGVPFCQSGMTI 60 Query: 95 SGGTSGCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXXXXXXXXXXXXXXXXXX 154 G TSGCP++NLIPEWN+L+Y G W++A RL Sbjct: 61 KGMTSGCPLNNLIPEWNDLLYTGNWEQAYHRLHKTSNFPEFTSRVCPALCEKACTCGLNG 120 Query: 155 XXI--KTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVTV 212 + K E+AII+ + +G+ NPP+ RT K +A+V H+VTV Sbjct: 121 KAVCTKENEMAIIEHAYAKGYAKANPPKVRTKKRIAVVGSGPAGLAVADQLNQRGHSVTV 180 Query: 213 FERSDRIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYDA 272 +ER+DRIGGLL YGIP MKL+K ++ R++D++ EG++FV N +G++I A++L+ +YDA Sbjct: 181 YERADRIGGLLRYGIPNMKLEKHIIDRKLDIMKEEGIEFVTNANVGENIKAKKLMADYDA 240 Query: 273 VVLCGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKLEDGNYVSAAGKDVIVLGG 332 VVL GA R N G + NG+ +A+D+L T KS L+S L+DG Y+SA K+VIV+GG Sbjct: 241 VVLACGAANPRDINAPGRDANGIYFAVDFLTATTKSLLDSGLKDGRYISAKDKNVIVIGG 300 Query: 333 GDTGSDCVATALRHGCSSITQF 354 GDTG+DCV T +RHGC S+TQ Sbjct: 301 GDTGNDCVGTCIRHGCKSVTQL 322 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,046,682,205 Number of extensions: 85355453 Number of successful extensions: 208657 Number of sequences better than 1.0e-05: 2243 Number of HSP's gapped: 203910 Number of HSP's successfully gapped: 2979 Length of query: 360 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 223 Effective length of database: 1,770,017,148 Effective search space: 394713824004 Effective search space used: 394713824004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)