BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0057.p (163 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2B1Y2.1 Q2B1Y2_9BACI Dihydrofolate reductase 187 4e-46 B3K6M7.1 B3K6M7_9BACI Dihydrofolate reductase EC=1.5.1.3 184 2e-45 B8I0T9.1 B8I0T9_CLOCE Dihydrofolate reductase region 182 7e-45 C5DAR3.1 C5DAR3_9BACI Dihydrofolate reductase EC=1.5.1.3 182 1e-44 A6CH84.1 A6CH84_9BACI Dihydrofolate reductase 177 3e-43 >Q2B1Y2.1 Q2B1Y2_9BACI Dihydrofolate reductase Length = 165 Score = 187 bits (474), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 91/161 (56%), Positives = 111/161 (68%), Gaps = 3/161 (1%) Query: 3 ISLIWAMAANGVIGKDNDMPWHLPRDFDYFKSQTLGKRMLMGRKTWDSLGGKPLKGRTSI 62 ISL+WAM N IGK+ND+PW LP D YFK T+G + MGRKTWDS+G +PL GR +I Sbjct: 2 ISLLWAMDENSAIGKNNDLPWRLPEDLKYFKRVTMGHSIAMGRKTWDSIG-RPLPGRENI 60 Query: 63 VLTRDRSFAPEGAEIVHSLEEAVAEG--RRDDELMVIGGAEIYKMMLPYADKLLVTRIAQ 120 VLTRDR F EG + +S ++ +AE + D E+ +IGGAEI+KM LP AD+L +T I Q Sbjct: 61 VLTRDRGFTCEGCTVFYSFDDFLAECSQKNDQEIFIIGGAEIFKMALPSADRLYLTMIHQ 120 Query: 121 DFEGDTRFPEVDWSGWREISNSPGIRDEKNPYDYRFYVYER 161 FE DT FPEVDW W+ S G DEKNPYDY F VY R Sbjct: 121 KFEADTFFPEVDWKEWQLESRENGKSDEKNPYDYEFLVYTR 161 >B3K6M7.1 B3K6M7_9BACI Dihydrofolate reductase EC=1.5.1.3 Length = 162 Score = 184 bits (468), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 89/161 (55%), Positives = 114/161 (70%), Gaps = 3/161 (1%) Query: 3 ISLIWAMAANGVIGKDNDMPWHLPRDFDYFKSQTLGKRMLMGRKTWDSLGGKPLKGRTSI 62 IS+IWAM + VIG + MPW LPRD +FK +TL K +LMGRKTWDS GGKPL R +I Sbjct: 2 ISMIWAMGKDQVIGLNGTMPWRLPRDMAFFKEKTLHKTILMGRKTWDSFGGKPLPHRKNI 61 Query: 63 VLTRDRSFA--PEGAEIVHSLEEAVAEGRRDDELMVIGGAEIYKMMLPYADKLLVTRIAQ 120 VLTRD SF + ++H +E A+ + ++ELMVIGG++IY++MLP AD+L T I + Sbjct: 62 VLTRDESFTLTEDQGIVIHDIENALPYSK-NEELMVIGGSQIYELMLPKADRLYCTFIDE 120 Query: 121 DFEGDTRFPEVDWSGWREISNSPGIRDEKNPYDYRFYVYER 161 FEGDT FPEV+W W+ + PGI DEKNPY YRF +ER Sbjct: 121 SFEGDTYFPEVNWDEWKIVEEVPGITDEKNPYAYRFVTFER 161 >B8I0T9.1 B8I0T9_CLOCE Dihydrofolate reductase region Length = 161 Score = 182 bits (463), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 85/159 (53%), Positives = 119/159 (74%), Gaps = 2/159 (1%) Query: 3 ISLIWAMAANGVIGKDNDMPWHLPRDFDYFKSQTLGKRMLMGRKTWDSLGGKPLKGRTSI 62 IS+IWAM N +G N MPW++P DF YFK T+GK ++MGRKT++S+G KPL GR +I Sbjct: 2 ISMIWAMGRNNALGCKNRMPWYIPADFAYFKKVTMGKPVIMGRKTFESIG-KPLPGRKNI 60 Query: 63 VLTRDRSFAPEGAEIVHSLEEAVAEGRRDDELMVIGGAEIYKMMLPYADKLLVTRIAQDF 122 V+TRD + P+G +V+S+E+A+ E + E+ +IGGAEIYK LP AD+L +T I ++F Sbjct: 61 VITRDTGYDPQGCIVVNSIEKAM-EYTEEKEVFIIGGAEIYKEFLPIADRLYITLIEKEF 119 Query: 123 EGDTRFPEVDWSGWREISNSPGIRDEKNPYDYRFYVYER 161 E D FPE+D+S W++IS GI+DEKNPY+Y++ VYER Sbjct: 120 EADAFFPEIDYSKWKQISCETGIKDEKNPYEYKWLVYER 158 >C5DAR3.1 C5DAR3_9BACI Dihydrofolate reductase EC=1.5.1.3 Length = 162 Score = 182 bits (461), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 86/161 (53%), Positives = 120/161 (74%), Gaps = 4/161 (2%) Query: 3 ISLIWAMAANGVIGKDNDMPWHLPRDFDYFKSQTLGKRMLMGRKTWDSLGGKPLKGRTSI 62 IS I AM N VIGKDN +PWHLP D YFK T+G ++MGRKT++S+G +PL GR ++ Sbjct: 2 ISHIVAMDQNRVIGKDNRLPWHLPADLAYFKKVTMGHAIIMGRKTFESIG-RPLPGRENV 60 Query: 63 VLTRDRSFAPEGAEIVHSLEEAVAE--GRRDDELMVIGGAEIYKMMLPYADKLLVTRIAQ 120 ++TR+RSF EG ++HS+E A+ + +RDDE+ VIGGAE+++ LP+AD+L +T+I Sbjct: 61 IVTRNRSFQAEGCTVIHSIE-AIRQFAAKRDDEVFVIGGAELFQATLPFADRLYITKIEA 119 Query: 121 DFEGDTRFPEVDWSGWREISNSPGIRDEKNPYDYRFYVYER 161 +F GDT +P+ + S WR +S + GI+DEKNPYDY F VYER Sbjct: 120 NFPGDTFYPDFEESEWRLVSYTKGIKDEKNPYDYAFIVYER 160 >A6CH84.1 A6CH84_9BACI Dihydrofolate reductase Length = 162 Score = 177 bits (448), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 82/160 (51%), Positives = 114/160 (71%), Gaps = 2/160 (1%) Query: 3 ISLIWAMAANGVIGKDNDMPWHLPRDFDYFKSQTLGKRMLMGRKTWDSLGGKPLKGRTSI 62 IS +WAM NGVIG +N +PW LP D +FK T+G ++MGRKT++S+G KPL GR +I Sbjct: 2 ISFVWAMDENGVIGFENKLPWRLPEDLKFFKETTMGHPIVMGRKTFESIG-KPLPGRENI 60 Query: 63 VLTRDRSFAPEGAEIVHSLEEAVAEGR-RDDELMVIGGAEIYKMMLPYADKLLVTRIAQD 121 +LTRD S+ EG I H +EE + ++ E+MV GG+E++K +PYADKL VTRI + Sbjct: 61 ILTRDISYEAEGCIIYHEIEEVLHYAEDKEVEVMVTGGSEVFKKFIPYADKLYVTRIYES 120 Query: 122 FEGDTRFPEVDWSGWREISNSPGIRDEKNPYDYRFYVYER 161 FEGDT FPE++W ++ IS G+++E+NPY Y F VYE+ Sbjct: 121 FEGDTYFPELNWDEYKLISTKQGVQNEQNPYHYVFEVYEK 160 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,063,259,444 Number of extensions: 43062101 Number of successful extensions: 112059 Number of sequences better than 1.0e-05: 1737 Number of HSP's gapped: 109566 Number of HSP's successfully gapped: 1737 Length of query: 163 Length of database: 3,060,696,203 Length adjustment: 123 Effective length of query: 40 Effective length of database: 1,901,911,358 Effective search space: 76076454320 Effective search space used: 76076454320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)