BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0055.p (204 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6M8.1 B3K6M8_9BACI Thymidylate synthase EC=2.1.1.45 249 1e-64 C1D070.1 C1D070_DEIDV Putative thymidylate synthase 231 4e-59 A6EGV1.1 A6EGV1_9SPHI Thymidylate synthase EC=2.1.1.45 229 9e-59 A0KG32.1 TYSY_AERHH Thymidylate synthase EC=2.1.1.45 228 2e-58 Q5ZRL3.1 TYSY_LEGPH Thymidylate synthase EC=2.1.1.45 226 6e-58 >B3K6M8.1 B3K6M8_9BACI Thymidylate synthase EC=2.1.1.45 Length = 264 Score = 249 bits (636), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 118/157 (75%), Positives = 132/157 (84%), Gaps = 6/157 (3%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 +++YL+LLQDILDHG K DRTGTGTLSVFGRQLRFDL++GFPL+TTKRIHLKSV+HELL Sbjct: 1 MKQYLELLQDILDHGVEKGDRTGTGTLSVFGRQLRFDLSQGFPLMTTKRIHLKSVVHELL 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFL G+TN YLKE+GV IWDEWAD NG+LGPVYGSQWRAWE+ DGRHIDQIA VIDSIK Sbjct: 61 WFLSGETNIRYLKENGVRIWDEWADENGDLGPVYGSQWRAWETPDGRHIDQIAGVIDSIK 120 Query: 128 NNPDSRRHIVSAWNVGEXXXXRADEAAALPFCISVLR 164 NNPDSRRHIVSAWNV E + LP C V + Sbjct: 121 NNPDSRRHIVSAWNVAEI------DNMKLPPCHFVFQ 151 >C1D070.1 C1D070_DEIDV Putative thymidylate synthase Length = 264 Score = 231 bits (588), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 104/152 (68%), Positives = 125/152 (82%), Gaps = 6/152 (3%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 +++YLD ++ +L+HG K+DRTGTGTLSVFG Q+RFDL EGFPLVTTKR HLKS+IHELL Sbjct: 1 MQQYLDFMRHVLEHGTEKTDRTGTGTLSVFGHQMRFDLQEGFPLVTTKRTHLKSIIHELL 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFL+G +N +YL+EHGV+IWDEWADP+GELGPVYG QWR+W + DGRHIDQIA V++ I+ Sbjct: 61 WFLQGSSNVAYLREHGVTIWDEWADPDGELGPVYGVQWRSWPTPDGRHIDQIAQVVEQIR 120 Query: 128 NNPDSRRHIVSAWNVGEXXXXRADEAAALPFC 159 PDSRR IVSAWNVGE E ALP C Sbjct: 121 RTPDSRRLIVSAWNVGEI------EQMALPPC 146 >A6EGV1.1 A6EGV1_9SPHI Thymidylate synthase EC=2.1.1.45 Length = 264 Score = 229 bits (585), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 102/152 (67%), Positives = 125/152 (82%), Gaps = 6/152 (3%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 +++YLDL+Q +LDHG K DRTGTGT+SVFG Q+RF+L EGFP+VTTK++HLKS+IHEL+ Sbjct: 1 MKQYLDLMQHVLDHGTQKHDRTGTGTISVFGYQMRFNLQEGFPMVTTKKLHLKSIIHELI 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFL GDTN YLK++GV IWDEWAD NG LGPVYGSQWR+W + DGRHIDQI+ ++++IK Sbjct: 61 WFLSGDTNIKYLKDNGVKIWDEWADENGNLGPVYGSQWRSWPTPDGRHIDQISQIVNTIK 120 Query: 128 NNPDSRRHIVSAWNVGEXXXXRADEAAALPFC 159 NNPDSRR IVSAWNV + E ALP C Sbjct: 121 NNPDSRRIIVSAWNVADI------EEMALPPC 146 >A0KG32.1 TYSY_AERHH Thymidylate synthase EC=2.1.1.45 Length = 264 Score = 228 bits (581), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 104/137 (75%), Positives = 120/137 (87%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 +R YLDL+Q ILD G KSDRTGTGT+S+FG Q+RF+LAEGFPLVTTK+ HL+S+IHELL Sbjct: 1 MRAYLDLMQKILDEGTVKSDRTGTGTISLFGHQMRFNLAEGFPLVTTKKCHLRSIIHELL 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFL GDTNT+YLKEHGVSIWDEWAD NG+LGPVYG+QWR+W +ADG IDQI +D IK Sbjct: 61 WFLNGDTNTAYLKEHGVSIWDEWADENGDLGPVYGAQWRSWPAADGSVIDQIQKAVDDIK 120 Query: 128 NNPDSRRHIVSAWNVGE 144 +NPDSRR IVSAWNVGE Sbjct: 121 HNPDSRRIIVSAWNVGE 137 >Q5ZRL3.1 TYSY_LEGPH Thymidylate synthase EC=2.1.1.45 Length = 264 Score = 226 bits (577), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 103/150 (68%), Positives = 125/150 (83%), Gaps = 5/150 (3%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 ++ YL LL+ IL HG KSDRTGTGTLSVFG Q+RFDLA+GFPLVTTK++H +S++HELL Sbjct: 1 MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAQGFPLVTTKKLHTRSIVHELL 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFL+GDTN SYLKE+GV+IWDEWAD NG+LGPVYG QWR+W +ADGR IDQ++ V+ IK Sbjct: 61 WFLRGDTNISYLKENGVTIWDEWADNNGDLGPVYGKQWRSWPTADGRTIDQLSEVVQQIK 120 Query: 128 NNPDSRRHIVSAWNVGEXXXXRADEAAALP 157 +NPDSRR IVSAWNVGE D+ A +P Sbjct: 121 SNPDSRRLIVSAWNVGEL-----DKMALMP 145 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,343,983,988 Number of extensions: 54711760 Number of successful extensions: 111315 Number of sequences better than 1.0e-05: 1419 Number of HSP's gapped: 110040 Number of HSP's successfully gapped: 1604 Length of query: 204 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 75 Effective length of database: 1,845,385,268 Effective search space: 138403895100 Effective search space used: 138403895100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)