BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0050.p (356 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6N0.1 B3K6N0_9BACI Catalytic domain of component of various d... 181 1e-43 B1DLS1.1 B1DLS1_9BACL Catalytic domain of components of various ... 161 1e-37 A8VYU7.1 A8VYU7_9BACI Dihydrolipoyllysine-residue (2-methylpropa... 158 7e-37 A8U8B5.1 A8U8B5_9LACT Dihydrolipoamide acetyltransferase 139 3e-31 Q92CX5.1 Q92CX5_LISIN PdhC protein 132 4e-29 >B3K6N0.1 B3K6N0_9BACI Catalytic domain of component of various dehydrogenase complexes Length = 440 Score = 181 bits (458), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 97/171 (56%), Positives = 107/171 (62%), Gaps = 20/171 (11%) Query: 120 FEYKFPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAK 179 FEY+FP IIKMHIK GDK+TDDDIIMEVQNDKAVVEVP PVNGTV EVFAK Sbjct: 4 FEYRFPELGEGLHEGEIIKMHIKPGDKVTDDDIIMEVQNDKAVVEVPCPVNGTVQEVFAK 63 Query: 180 DGQVCRVGEVVAIIAAEGDVPQQEGGHXXXXXXXXXXXXXXXXXXXX------------- 226 DGQVCRVGEVVAII AEGD+P+QE Sbjct: 64 DGQVCRVGEVVAIIDAEGDIPEQEAPAEEQSAQEADAAKGSADTTSSPAQDAPADAKQGG 123 Query: 227 -------XXXNRDVLATPSVRKFARDQSVDISKVNGTGKNGKITHEDVEAF 270 NR+VLATPSVRKFAR+Q VDI++V G+G NGK+T EDVEAF Sbjct: 124 NGEAATPAAPNREVLATPSVRKFAREQGVDIAQVQGSGNNGKVTREDVEAF 174 Score = 148 bits (373), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 72/88 (81%), Positives = 74/88 (84%) Query: 1 VAKFEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEV 60 +AKFEYRFP IIKMHIK GDKVTDDDI+MEVQNDKAVVEVPCPVNGTV EV Sbjct: 1 MAKFEYRFPELGEGLHEGEIIKMHIKPGDKVTDDDIIMEVQNDKAVVEVPCPVNGTVQEV 60 Query: 61 FTKDGQVCRVGEVVAIIDAEGDIPEQEG 88 F KDGQVCRVGEVVAIIDAEGDIPEQE Sbjct: 61 FAKDGQVCRVGEVVAIIDAEGDIPEQEA 88 >B1DLS1.1 B1DLS1_9BACL Catalytic domain of components of various dehydrogenase complexes Length = 434 Score = 161 bits (407), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 90/160 (56%), Positives = 100/160 (62%), Gaps = 12/160 (7%) Query: 120 FEYKFPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAK 179 FEYKFP I+K+HIKAGDK+TDDDIIMEVQNDKAVVEVP PVNGTVLEV K Sbjct: 4 FEYKFPELGEGLHEGEIVKVHIKAGDKVTDDDIIMEVQNDKAVVEVPCPVNGTVLEVRMK 63 Query: 180 DGQVCRVGEVVAIIAAEGDVPQQEGGHXXXXXXXXXXXXXXXXXXXXXXXNRD------- 232 DGQVC VGEVVAII AEGD+P+Q+ Sbjct: 64 DGQVCHVGEVVAIIDAEGDIPEQDTPAAEAPAAAPAPAAAPAAPAAAPAAAPAAPAEAPK 123 Query: 233 -----VLATPSVRKFARDQSVDISKVNGTGKNGKITHEDV 267 VLATPSVRKFAR+Q VD++ V GTGKNG+IT EDV Sbjct: 124 ATGGLVLATPSVRKFAREQGVDLTTVTGTGKNGRITREDV 163 Score = 139 bits (351), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 68/87 (78%), Positives = 74/87 (85%) Query: 1 VAKFEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEV 60 +AKFEY+FP I+K+HIKAGDKVTDDDI+MEVQNDKAVVEVPCPVNGTVLEV Sbjct: 1 MAKFEYKFPELGEGLHEGEIVKVHIKAGDKVTDDDIIMEVQNDKAVVEVPCPVNGTVLEV 60 Query: 61 FTKDGQVCRVGEVVAIIDAEGDIPEQE 87 KDGQVC VGEVVAIIDAEGDIPEQ+ Sbjct: 61 RMKDGQVCHVGEVVAIIDAEGDIPEQD 87 >A8VYU7.1 A8VYU7_9BACI Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase EC=2.3.1.168 Length = 542 Score = 158 bits (400), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 93/272 (34%), Positives = 142/272 (52%), Gaps = 12/272 (4%) Query: 4 FEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEVFTK 63 +E++ P I K ++K GD++ +DD++ EVQNDKAVVE+P PV+G + ++ + Sbjct: 3 YEFKLPDIGEGIHEGEIAKWNVKEGDEIKEDDVLCEVQNDKAVVEIPSPVDGKIAKIHVE 62 Query: 64 DGQVCRVGEVVAIIDAEGDIPEQEGGHXXXXXXXXXXXXKGGXXXXXXXXXXXXXNFEYK 123 +G V VG V+ + + + PE G + +E+K Sbjct: 63 EGVVTEVGTVIVSFETDAEQPEDAHGKEEEQEEASSSETE------STSTSSKSSGYEFK 116 Query: 124 FPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAKDGQV 183 P + K ++K GD++ +DD++ EVQNDKAVVE+PSPV+GTV ++ ++G V Sbjct: 117 LPDIGEGIHEGEVAKWNVKEGDEVKEDDVLCEVQNDKAVVEIPSPVDGTVKKIHVEEGVV 176 Query: 184 CRVGEVVAIIAAEGDVPQQEGGHXXXXXXXXXXXXXXXXXXXXXX----XNRDVLATPSV 239 VG+V II + D Q E H NR V+A PSV Sbjct: 177 INVGDV--IITFDTDAEQPEDAHGSSGEEAPKTDDKAPKSTAKSSEPLDENRRVIAMPSV 234 Query: 240 RKFARDQSVDISKVNGTGKNGKITHEDVEAFL 271 RKFAR++ VDI +V G+GKNG++ ED+E F+ Sbjct: 235 RKFAREKDVDIRQVRGSGKNGRVLKEDIETFV 266 >A8U8B5.1 A8U8B5_9LACT Dihydrolipoamide acetyltransferase Length = 533 Score = 139 bits (351), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 85/271 (31%), Positives = 137/271 (50%), Gaps = 18/271 (6%) Query: 4 FEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEVFTK 63 F+++ P I+K + GD + ++D ++E+QNDK+V E+ PV+GTV ++ + Sbjct: 3 FKFKLPDVGEGMAEGEIVKWLVAEGDTIEEEDSIVEIQNDKSVEEIATPVSGTVKKIMVE 62 Query: 64 DGQVCRVGEVVAIIDAEGDIPEQEGGHXXXXXXXXXXXXKGGXXXXXXXXXXXXXNFEYK 123 +G V VG+V+ IDA G E+E G F++K Sbjct: 63 EGTVATVGQVIIEIDAPGYEDEEEAAPAASTPEPAAPASSGTSF------------FQFK 110 Query: 124 FPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAKDGQV 183 P I+K + GD + ++D + E+QNDK+V E+ +PV+GT+ ++ ++G V Sbjct: 111 MPDVGEGMAEGEIVKWLVAEGDTVNEEDSVAEIQNDKSVEEIATPVSGTIKKIMVEEGTV 170 Query: 184 CRVGEVVAIIAAEGDVPQQ--EGGHXXXXXXXXXXXXXXXXXXXXXXXNRDVLATPSVRK 241 VG+V+ I D P+ +G N++VLA PSVR+ Sbjct: 171 ALVGQVLIEI----DSPEHNPKGSAAPVAQEAPAAETSTSAATPAATSNKNVLAMPSVRQ 226 Query: 242 FARDQSVDISKVNGTGKNGKITHEDVEAFLK 272 FAR+ +VDI++V TGKNG+ T ED+E F K Sbjct: 227 FARENNVDITQVTATGKNGRTTKEDIENFKK 257 Score = 60.1 bits (144), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 30/79 (37%), Positives = 49/79 (62%) Query: 119 NFEYKFPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFA 178 +F++K P I+K + GD I ++D I+E+QNDK+V E+ +PV+GTV ++ Sbjct: 2 SFKFKLPDVGEGMAEGEIVKWLVAEGDTIEEEDSIVEIQNDKSVEEIATPVSGTVKKIMV 61 Query: 179 KDGQVCRVGEVVAIIAAEG 197 ++G V VG+V+ I A G Sbjct: 62 EEGTVATVGQVIIEIDAPG 80 >Q92CX5.1 Q92CX5_LISIN PdhC protein Length = 544 Score = 132 bits (333), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 85/280 (30%), Positives = 131/280 (46%), Gaps = 20/280 (7%) Query: 4 FEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEVFTK 63 + ++ P I+K ++ GDK+ +D+ + EVQNDK+V E+ PV+GT+ E+ Sbjct: 3 YSFKLPDIGEGIHEGEIVKWFVQPGDKIEEDESLFEVQNDKSVEEITSPVSGTIKEIKVA 62 Query: 64 DGQVCRVGEVVAIIDAEGDIPEQEGGHXXXXXXXXXXXXKGGXXXXXXXXXXXXXNFEYK 123 +G V VG+V+ D EG + FE+K Sbjct: 63 EGTVATVGQVLVTFDG------VEGHEDDAEEESAAPKAESTESTPAPAQASGKGIFEFK 116 Query: 124 FPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAKDGQV 183 P I+K I+ GDK+ +D I EVQNDK+V E+ SPV+GTV ++ +G V Sbjct: 117 LPDIGEGIHEGEIVKWFIQPGDKVEEDQSIFEVQNDKSVEEITSPVDGTVKDILVSEGTV 176 Query: 184 CRVGEVVAIIAAEGDV------------PQQEGGHXXXXXXXXXXXXXXXXXXXXXXXNR 231 VG+V ++ EGD P +E N Sbjct: 177 ATVGQV--LVTFEGDFEGEASHESTPESPAEEAELTNNDATSAPATGGNGTPSSKKDPNG 234 Query: 232 DVLATPSVRKFARDQSVDISKVNGTGKNGKITHEDVEAFL 271 V+A PSVRK+AR++ V+I++V G+GKN ++ D++AFL Sbjct: 235 LVIAMPSVRKYAREKDVNIAEVAGSGKNNRVVKADIDAFL 274 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,547,045,972 Number of extensions: 50390561 Number of successful extensions: 135219 Number of sequences better than 1.0e-05: 809 Number of HSP's gapped: 134916 Number of HSP's successfully gapped: 1176 Length of query: 356 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 219 Effective length of database: 1,770,017,148 Effective search space: 387633755412 Effective search space used: 387633755412 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)