BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0049.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6N1.1 B3K6N1_9BACI Transketolase central region 473 e-132 B1DLS0.1 B1DLS0_9BACL Transketolase central region 459 e-127 C0ZER5.1 C0ZER5_BREBN Pyruvate dehydrogenase E1 component beta s... 399 e-109 A8VYU8.1 A8VYU8_9BACI Virulence-associated E family protein 375 e-102 C4L5B5.1 C4L5B5_EXISA Transketolase central region 374 e-102 >B3K6N1.1 B3K6N1_9BACI Transketolase central region Length = 325 Score = 473 bits (1217), Expect = e-132, Method: Compositional matrix adjust. Identities = 225/268 (83%), Positives = 252/268 (94%) Query: 6 LNPQSAGWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRT 65 L + G + G+QGFRPIAEIQFVGFIFEALDQIVVQAAR+R+RSGGR+N+P+VFRT Sbjct: 58 LAESAIGGLAVGLGIQGFRPIAEIQFVGFIFEALDQIVVQAARMRYRSGGRYNSPIVFRT 117 Query: 66 PFGGGVKAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLY 125 PFGGGVKAAELHTD+LEGLI Q+PGIKVV+PSNPYDAKGL+IA+IRDNDPVFFMEHLNLY Sbjct: 118 PFGGGVKAAELHTDALEGLITQTPGIKVVVPSNPYDAKGLMIAAIRDNDPVFFMEHLNLY 177 Query: 126 HAFRAEVPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLR 185 HAFRAEVPEG+YTVELGKANVVREG+DV+II YGLMVHTATKAA++LEK+GIKAEIIDLR Sbjct: 178 HAFRAEVPEGDYTVELGKANVVREGADVTIIAYGLMVHTATKAADELEKNGIKAEIIDLR 237 Query: 186 TVSPIDIDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDT 245 T+SPIDIDT++ASVKKTNRAIVVQEAQKS+GVAAEVIAQINEKAILHLEAPVLRV PDT Sbjct: 238 TISPIDIDTVLASVKKTNRAIVVQEAQKSAGVAAEVIAQINEKAILHLEAPVLRVTPPDT 297 Query: 246 VYPFAQIEDTWIPTPARVIAAVKKVMEF 273 VYPFAQIEDTW+PTPAR++ AV KV+ F Sbjct: 298 VYPFAQIEDTWLPTPARIVDAVNKVLNF 325 >B1DLS0.1 B1DLS0_9BACL Transketolase central region Length = 325 Score = 459 bits (1180), Expect = e-127, Method: Compositional matrix adjust. Identities = 213/268 (79%), Positives = 247/268 (92%) Query: 6 LNPQSAGWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRT 65 L + G + G+QGFRP+AEIQFVGFI+EA+DQ+ +QAAR+R+RSGGR+N+P+VFRT Sbjct: 58 LAESAIGGLAVGMGIQGFRPVAEIQFVGFIYEAMDQMFIQAARMRYRSGGRYNSPIVFRT 117 Query: 66 PFGGGVKAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLY 125 PFGGGVKAAELHTDSLEGL Q+PGIKVVIPSNPYDAKGL+IA+IRDNDPVFFMEHLNLY Sbjct: 118 PFGGGVKAAELHTDSLEGLAVQTPGIKVVIPSNPYDAKGLMIAAIRDNDPVFFMEHLNLY 177 Query: 126 HAFRAEVPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLR 185 AFRAEVPEGEYT+E+GKANVVREGSDV+II YG+MVHTA KAA++L K+G+ AE+IDLR Sbjct: 178 RAFRAEVPEGEYTIEIGKANVVREGSDVTIIAYGMMVHTAVKAADELAKNGVNAEVIDLR 237 Query: 186 TVSPIDIDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDT 245 ++ P+DIDTIVAS+KKTNRAIVVQEAQK+SGVAAEVIAQINEKAILHLEAPVLRVAGPDT Sbjct: 238 SLVPLDIDTIVASIKKTNRAIVVQEAQKTSGVAAEVIAQINEKAILHLEAPVLRVAGPDT 297 Query: 246 VYPFAQIEDTWIPTPARVIAAVKKVMEF 273 VYPFAQIEDTW+PTPAR++ AV KV+ F Sbjct: 298 VYPFAQIEDTWLPTPARIVDAVNKVLSF 325 >C0ZER5.1 C0ZER5_BREBN Pyruvate dehydrogenase E1 component beta subunit EC=1.2.4.1 Length = 326 Score = 399 bits (1024), Expect = e-109, Method: Compositional matrix adjust. Identities = 183/263 (69%), Positives = 223/263 (84%), Gaps = 1/263 (0%) Query: 12 GWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGV 71 G + + GFRP+AEIQF GF+FE D + Q++R+R+RSGGRF++P+ FR+PFGGGV Sbjct: 64 GGLAVGLSINGFRPVAEIQFFGFVFETFDAVASQSSRMRYRSGGRFSSPITFRSPFGGGV 123 Query: 72 KAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAE 131 K ELH DSLEGL+ Q+PG+KVVIPSNPYDAKGLLI++IRDNDPV F+EH+ LY +FR E Sbjct: 124 KTPELHADSLEGLMLQTPGLKVVIPSNPYDAKGLLISAIRDNDPVVFLEHMKLYRSFRQE 183 Query: 132 VPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEK-DGIKAEIIDLRTVSPI 190 VPEGEYT+ LGKANVV+EGSDV+IITYG MVHT+ KAAE++EK G K E+IDLRT+SP+ Sbjct: 184 VPEGEYTIPLGKANVVKEGSDVTIITYGAMVHTSLKAAEEIEKARGAKVEVIDLRTISPL 243 Query: 191 DIDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFA 250 DIDTIVASVKKTNRAIVVQEAQK+SGVAAE+I QINE+AILHLEAPVLR+ PDTVYPFA Sbjct: 244 DIDTIVASVKKTNRAIVVQEAQKTSGVAAEIITQINERAILHLEAPVLRITAPDTVYPFA 303 Query: 251 QIEDTWIPTPARVIAAVKKVMEF 273 Q ED W+P RV+ + +V++F Sbjct: 304 QAEDVWLPDVKRVVDGLTQVLDF 326 >A8VYU8.1 A8VYU8_9BACI Virulence-associated E family protein Length = 325 Score = 375 bits (963), Expect = e-102, Method: Compositional matrix adjust. Identities = 174/262 (66%), Positives = 212/262 (80%) Query: 12 GWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGV 71 G + GV G+RP+ EIQF GF+FE D I Q AR+R+RSGG +++PV R+PFGGGV Sbjct: 64 GGLATGLGVTGYRPVMEIQFFGFVFETFDAIAAQMARMRYRSGGVYHSPVTIRSPFGGGV 123 Query: 72 KAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAE 131 K ELH DSLEGL+AQSPGIKVVIPS PYDAKGLLI++IRDNDPV F+EH+ LY +FR E Sbjct: 124 KTPELHADSLEGLMAQSPGIKVVIPSGPYDAKGLLISAIRDNDPVVFLEHMKLYRSFREE 183 Query: 132 VPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPID 191 VPE EYT+ LGKANV +EG+DV+IITYG MV + KAAEQLEKDG+ AE+IDLRT+SP+D Sbjct: 184 VPEEEYTLPLGKANVKKEGTDVTIITYGAMVQASMKAAEQLEKDGVAAEVIDLRTISPLD 243 Query: 192 IDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQ 251 +DTI+ SVKKTNRA+VVQEAQK +G+AA V+A+IN++AIL LEAPV RV PDTV+PFA Sbjct: 244 VDTIIESVKKTNRAVVVQEAQKQAGIAANVVAEINDRAILSLEAPVKRVTAPDTVFPFAS 303 Query: 252 IEDTWIPTPARVIAAVKKVMEF 273 EDTW+P +I AV V+ F Sbjct: 304 AEDTWLPNHKDIIEAVNGVINF 325 >C4L5B5.1 C4L5B5_EXISA Transketolase central region Length = 325 Score = 374 bits (959), Expect = e-102, Method: Compositional matrix adjust. Identities = 174/251 (69%), Positives = 207/251 (82%) Query: 23 FRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGVKAAELHTDSLE 82 FRPI E+QF GF+FE D I Q AR+R+RSGG ++ P+ R+PFGGGVK ELH DSLE Sbjct: 75 FRPIMEVQFFGFVFEVFDSIAAQMARMRYRSGGAYSQPITIRSPFGGGVKTPELHADSLE 134 Query: 83 GLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAEVPEGEYTVELG 142 GL+AQ+PG+KVVIPS PYDAKGLLIASIRDNDPV F+EH+ LY +FR EVPEGEYT+ELG Sbjct: 135 GLMAQTPGLKVVIPSTPYDAKGLLIASIRDNDPVVFLEHMKLYRSFRGEVPEGEYTIELG 194 Query: 143 KANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPIDIDTIVASVKKT 202 KA+V REG+DVSIITYG MVHT+ KAAE+LEK+ I E+IDL TVSP+DIDTIV SVKKT Sbjct: 195 KADVKREGTDVSIITYGAMVHTSLKAAEELEKENINVEVIDLMTVSPLDIDTIVESVKKT 254 Query: 203 NRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQIEDTWIPTPAR 262 RAIVVQEAQ+ +G+AA V+ +I E+AILHLEAPVLRV PDTV+ FAQ ED W+P Sbjct: 255 GRAIVVQEAQRQAGIAANVVTEIQERAILHLEAPVLRVTAPDTVFAFAQGEDMWLPDHKD 314 Query: 263 VIAAVKKVMEF 273 V+A VK+V+ F Sbjct: 315 VVAKVKEVVNF 325 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,553,945,973 Number of extensions: 60735317 Number of successful extensions: 166058 Number of sequences better than 1.0e-05: 3213 Number of HSP's gapped: 163475 Number of HSP's successfully gapped: 3221 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)