BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0048.p (358 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6N2.1 B3K6N2_9BACI Pyruvate dehydrogenase (Acetyl-transferrin... 618 e-175 B1DLR9.1 B1DLR9_9BACL Pyruvate dehydrogenase (Acetyl-transferrin... 489 e-136 C0ZER6.1 C0ZER6_BREBN Pyruvate dehydrogenase E1 component alpha ... 437 e-121 C3J0H1.1 C3J0H1_9BACI Pyruvate dehydrogenase (Acetyl-transferrin... 417 e-115 B7Y935.1 B7Y935_9BACI Pyruvate dehydrogenase (Acetyl-transferrin... 417 e-115 >B3K6N2.1 B3K6N2_9BACI Pyruvate dehydrogenase (Acetyl-transferring) E1 component, alpha subunit EC=1.2.4.1 Length = 355 Score = 618 bits (1593), Expect = e-175, Method: Compositional matrix adjust. Identities = 291/355 (81%), Positives = 316/355 (89%) Query: 4 MTKVPYEVYTEDVEALSVLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVNLG 63 MTKVPYEVYTE+V LSVLSPDG+++NKD +P L+DDQLKE+MYRMVFTRTWDDRAVNLG Sbjct: 1 MTKVPYEVYTEEVGTLSVLSPDGKIVNKDLLPELSDDQLKEIMYRMVFTRTWDDRAVNLG 60 Query: 64 RQGRLGFYAPVSGQEATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQH 123 RQGRLGFYAPVSGQEATM+GSEFAL+K+DF+CPGYRD+PQ+VWHGLPLYQAFLYSRGHQH Sbjct: 61 RQGRLGFYAPVSGQEATMVGSEFALEKDDFICPGYRDMPQIVWHGLPLYQAFLYSRGHQH 120 Query: 124 GGQIPEGVNVLMPQIIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGLNF 183 GGQIPEGVNVLMPQIIIGAQILHA GIAMG+KLKKQ+ V ITYTGDGGSSEGDFYEGLN+ Sbjct: 121 GGQIPEGVNVLMPQIIIGAQILHAMGIAMGYKLKKQKQVVITYTGDGGSSEGDFYEGLNY 180 Query: 184 AGRFKLPVIFFVQNNGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAVQX 243 AG +KLPVIFFVQNNGYAITTPF+KQT GI G+K+DGMDV AVI AVQ Sbjct: 181 AGVYKLPVIFFVQNNGYAITTPFSKQTAALSIAHKAVAAGIKGVKVDGMDVLAVIKAVQD 240 Query: 244 XXXXXXXXXXXTLIEAVTYRFRPHSLSDDASKYRSKEEEGQWNEKDPIARLAKYLEKKGL 303 TLIEAVTYRFRPHSLSDDA+KYR+KEEEG+WNEKDPIARLAKYLE KGL Sbjct: 241 AAERGRNGEGATLIEAVTYRFRPHSLSDDATKYRTKEEEGEWNEKDPIARLAKYLEAKGL 300 Query: 304 WTEEDTLRVREEAKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQKADFE 358 WTEEDT RV+EEAKA VNEQIKKAEQTEKMTVPGLIDSMFE TPKHLEEQKADF+ Sbjct: 301 WTEEDTARVKEEAKAKVNEQIKKAEQTEKMTVPGLIDSMFEQTPKHLEEQKADFQ 355 >B1DLR9.1 B1DLR9_9BACL Pyruvate dehydrogenase (Acetyl-transferring) EC=1.2.4.1 Length = 333 Score = 489 bits (1260), Expect = e-136, Method: Compositional matrix adjust. Identities = 230/331 (69%), Positives = 266/331 (80%) Query: 2 KMMTKVPYEVYTEDVEALSVLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVN 61 K++ K+PYEV TE V+ +SVLS DG+V+N + MP LTD+QLKE+MYRMVFTRTWD+RAVN Sbjct: 3 KVLNKLPYEVQTEPVKPISVLSLDGDVVNPEYMPDLTDEQLKEIMYRMVFTRTWDERAVN 62 Query: 62 LGRQGRLGFYAPVSGQEATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGH 121 LGRQGRLGFYAPVSGQEA+MIGSE+AL K+DF+CPGYRDIPQLVWHGLP+YQ FLYSRGH Sbjct: 63 LGRQGRLGFYAPVSGQEASMIGSEYALNKDDFICPGYRDIPQLVWHGLPMYQGFLYSRGH 122 Query: 122 QHGGQIPEGVNVLMPQIIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGL 181 QHGGQIPE V+VLMPQIIIGAQ+LHA G+AM FK + ++ VAITYTGDGGSSEGDFYE + Sbjct: 123 QHGGQIPEDVHVLMPQIIIGAQVLHAAGVAMAFKKRGEKRVAITYTGDGGSSEGDFYESM 182 Query: 182 NFAGRFKLPVIFFVQNNGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAV 241 NFAG FKLPVI+ VQNN YAITTP+ KQT GI GI+IDGMDV AVI AV Sbjct: 183 NFAGVFKLPVIYVVQNNRYAITTPYEKQTAALSVAHKSVAAGIKGIQIDGMDVLAVIKAV 242 Query: 242 QXXXXXXXXXXXXTLIEAVTYRFRPHSLSDDASKYRSKEEEGQWNEKDPIARLAKYLEKK 301 TLIE +TYRFRPHSLSDDA+KYR+KEEE +W KDP+ R K+LEKK Sbjct: 243 SEAAERGRNGEGATLIEMLTYRFRPHSLSDDATKYRTKEEEAEWGLKDPLIRFGKFLEKK 302 Query: 302 GLWTEEDTLRVREEAKATVNEQIKKAEQTEK 332 GLWTEEDT RV+EEAKA + +K +Q K Sbjct: 303 GLWTEEDTNRVKEEAKAALTRTSRKRKQLRK 333 >C0ZER6.1 C0ZER6_BREBN Pyruvate dehydrogenase E1 component alpha subunit EC=1.2.4.1 Length = 361 Score = 437 bits (1125), Expect = e-121, Method: Compositional matrix adjust. Identities = 207/346 (59%), Positives = 256/346 (73%), Gaps = 1/346 (0%) Query: 13 TEDVEALSVLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVNLGRQGRLGFYA 72 TE+ L +L+PDG V+ D MP L+DD+L+ELM +MVFTR WD RA++L RQGRLGFYA Sbjct: 10 TENNAPLQILAPDGTVVRPDLMPKLSDDELRELMRKMVFTRVWDQRAISLNRQGRLGFYA 69 Query: 73 PVSGQEATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQHGGQIPEGVN 132 PV+GQEA+MIGSE AL KEDFV P YRDIPQ+VWHG P+++AFLYSRGH GG+IPEGVN Sbjct: 70 PVAGQEASMIGSEAALSKEDFVLPSYRDIPQMVWHGYPMHKAFLYSRGHIEGGKIPEGVN 129 Query: 133 VLMPQIIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGLNFAGRFKLPVI 192 VLMPQIII AQ ATG+AMG+KL+ ++ VAI Y GDG +S+GDFYEG+NFAG +KLPVI Sbjct: 130 VLMPQIIIAAQCTQATGVAMGYKLRGEKKVAINYFGDGATSQGDFYEGMNFAGVYKLPVI 189 Query: 193 FFVQNNGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAVQXXXXXXXXXX 252 FF QNNGYAI+ PF KQT GI ++DGMD+ AV AVQ Sbjct: 190 FFSQNNGYAISLPFEKQTASENIAVKAVAAGIASERVDGMDILAVYYAVQQAKERGVNGE 249 Query: 253 XXTLIEAVTYRFRPHSLS-DDASKYRSKEEEGQWNEKDPIARLAKYLEKKGLWTEEDTLR 311 TLIEA+TYR+ PH+++ DD ++YR+ EE+ +W +DP+ R K+LE KGLW+E+D Sbjct: 250 GATLIEAMTYRYGPHTMAGDDPTRYRTGEEQSEWELRDPLIRFRKFLEAKGLWSEKDEEA 309 Query: 312 VREEAKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQKADF 357 V EEAKA V + IKKA++T KM V LID MFE P LEEQKA+F Sbjct: 310 VIEEAKAAVADAIKKADETPKMKVSELIDVMFETLPPALEEQKAEF 355 >C3J0H1.1 C3J0H1_9BACI Pyruvate dehydrogenase (Acetyl-transferring) E1 component, alpha subunit EC=1.2.4.1 Length = 369 Score = 417 bits (1071), Expect = e-115, Method: Compositional matrix adjust. Identities = 195/345 (56%), Positives = 251/345 (72%), Gaps = 1/345 (0%) Query: 10 EVYTEDVEALSVLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVNLGRQGRLG 69 E E +L+ +GE++N++ MP L+D+QLKELM RMV+TR D R+++L RQGRLG Sbjct: 15 EKVAEQFPTFQILNEEGEIVNEEAMPELSDEQLKELMRRMVYTRILDQRSISLNRQGRLG 74 Query: 70 FYAPVSGQEATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQHGGQIPE 129 FYAP +GQEA+ I S FAL+KEDF+ PGYRD+PQ++WHGLPLYQAFL+SRGH HG QIPE Sbjct: 75 FYAPTAGQEASQIASHFALEKEDFILPGYRDVPQIIWHGLPLYQAFLFSRGHFHGNQIPE 134 Query: 130 GVNVLMPQIIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGLNFAGRFKL 189 GVNVL PQIIIGAQ + A G+A+G K++ ++ VAITYTGDGG+S+GDFYEG+NFAG FK Sbjct: 135 GVNVLPPQIIIGAQYIQAAGVALGLKMRGKKAVAITYTGDGGTSQGDFYEGINFAGAFKA 194 Query: 190 PVIFFVQNNGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAVQXXXXXXX 249 P IF VQNN +AI+TP KQT GIPGI++DGMD AV +AV+ Sbjct: 195 PAIFVVQNNRFAISTPVEKQTVAKTLAQKAVAAGIPGIQVDGMDPLAVYAAVKAARERAI 254 Query: 250 XXXXXTLIEAVTYRFRPHSLS-DDASKYRSKEEEGQWNEKDPIARLAKYLEKKGLWTEED 308 TLIE + +R+ PH++S DD ++YRSKE E +W +KDP+ R K+LE KGLW+EE+ Sbjct: 255 NGEGPTLIETLCFRYGPHTMSGDDPTRYRSKELENEWAKKDPLVRFRKFLEAKGLWSEEE 314 Query: 309 TLRVREEAKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQ 353 RV E+AK + E IKKA++T K V LI MFE P +L+EQ Sbjct: 315 ENRVIEQAKEDIKEAIKKADETPKQKVTDLISIMFEELPANLKEQ 359 >B7Y935.1 B7Y935_9BACI Pyruvate dehydrogenase (Acetyl-transferring) E1 component, alpha subunit EC=1.2.4.1 Length = 369 Score = 417 bits (1071), Expect = e-115, Method: Compositional matrix adjust. Identities = 195/345 (56%), Positives = 251/345 (72%), Gaps = 1/345 (0%) Query: 10 EVYTEDVEALSVLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVNLGRQGRLG 69 E E +L+ +GE++N++ MP L+D+QLKELM RMV+TR D R+++L RQGRLG Sbjct: 15 EKVAEQFPTFQILNEEGEIVNEEAMPELSDEQLKELMRRMVYTRILDQRSISLNRQGRLG 74 Query: 70 FYAPVSGQEATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQHGGQIPE 129 FYAP +GQEA+ I S FAL+KEDF+ PGYRD+PQ++WHGLPLYQAFL+SRGH HG QIPE Sbjct: 75 FYAPTAGQEASQIASHFALEKEDFILPGYRDVPQIIWHGLPLYQAFLFSRGHFHGNQIPE 134 Query: 130 GVNVLMPQIIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGLNFAGRFKL 189 GVNVL PQIIIGAQ + A G+A+G K++ ++ VAITYTGDGG+S+GDFYEG+NFAG FK Sbjct: 135 GVNVLPPQIIIGAQYIQAAGVALGLKMRGKKAVAITYTGDGGTSQGDFYEGINFAGAFKA 194 Query: 190 PVIFFVQNNGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAVQXXXXXXX 249 P IF VQNN +AI+TP KQT GIPGI++DGMD AV +AV+ Sbjct: 195 PAIFVVQNNRFAISTPVEKQTVAKTLAQKAVAAGIPGIQVDGMDPLAVYAAVKAARERAI 254 Query: 250 XXXXXTLIEAVTYRFRPHSLS-DDASKYRSKEEEGQWNEKDPIARLAKYLEKKGLWTEED 308 TLIE + +R+ PH++S DD ++YRSKE E +W +KDP+ R K+LE KGLW+EE+ Sbjct: 255 NGEGPTLIETLCFRYGPHTMSGDDPTRYRSKELENEWAKKDPLVRFRKFLEAKGLWSEEE 314 Query: 309 TLRVREEAKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQ 353 RV E+AK + E IKKA++T K V LI MFE P +L+EQ Sbjct: 315 ENRVIEQAKEDIKEAIKKADETPKQKVTDLISIMFEELPANLKEQ 359 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,030,438,269 Number of extensions: 83305908 Number of successful extensions: 216584 Number of sequences better than 1.0e-05: 2070 Number of HSP's gapped: 213262 Number of HSP's successfully gapped: 2074 Length of query: 358 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 221 Effective length of database: 1,770,017,148 Effective search space: 391173789708 Effective search space used: 391173789708 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)