BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0044.p (213 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6N6.1 B3K6N6_9BACI Thiamine pyrophosphokinase 282 2e-74 C0Z886.1 C0Z886_BREBN Thiamine pyrophosphokinase EC=2.7.6.2 219 1e-55 A4J9F1.1 A4J9F1_DESRM Thiamine pyrophosphokinase 219 2e-55 B1DLR5.1 B1DLR5_9BACL Thiamine pyrophosphokinase 196 1e-48 Q8ER19.1 Q8ER19_OCEIH Hypothetical conserved protein 171 3e-41 >B3K6N6.1 B3K6N6_9BACI Thiamine pyrophosphokinase Length = 214 Score = 282 bits (721), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 134/213 (62%), Positives = 169/213 (79%) Query: 1 MPTKRVVIFAGGDLSAEYLSLLDEEDFVIGADRGALFLITHGVEPDISVGDFDSVPPEAL 60 M RV+IF GG+LS +L+ + E DF+IGADRGALFLI HG++PD+SVGDFDS+PPE + Sbjct: 1 MKHTRVLIFTGGELSTGFLNEVQEGDFIIGADRGALFLIEHGIKPDLSVGDFDSIPPEQM 60 Query: 61 HQIEAGSKKTITCDPVDKDLTDSEMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTR 120 ++ + S K I CDP+DK+LTD+E+A +LA+ PESI++ G TGTR+DHTLA+I M+ R Sbjct: 61 DRVHSMSGKVIDCDPIDKNLTDTELAFELAMERSPESIMILGATGTRLDHTLANIHMLIR 120 Query: 121 ALQRQVHCSVMDTHNYITLTGSQTEIHDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATL 180 LQ + CS++D +N+ITLTGS + + G+TYVSLLPLT EVTGI LEGFQYPL ATL Sbjct: 121 GLQHHIPCSILDENNFITLTGSSCLVENKGFTYVSLLPLTTEVTGIYLEGFQYPLQDATL 180 Query: 181 KLGQSLGVSNILTAERGTVSIESGLLLIIQSKD 213 +LGQSLGVSN L E+GTV IE GLLLIIQSKD Sbjct: 181 RLGQSLGVSNRLAEEKGTVRIEGGLLLIIQSKD 213 >C0Z886.1 C0Z886_BREBN Thiamine pyrophosphokinase EC=2.7.6.2 Length = 213 Score = 219 bits (558), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 103/213 (48%), Positives = 149/213 (69%) Query: 1 MPTKRVVIFAGGDLSAEYLSLLDEEDFVIGADRGALFLITHGVEPDISVGDFDSVPPEAL 60 M R+++FAGG+L + + E D+++G DRGALFL+ +G+ P +S+GDFDSV E + Sbjct: 1 MNPTRILLFAGGNLGTWAIQEIRENDWLVGVDRGALFLVRNGLVPKLSIGDFDSVSSEEM 60 Query: 61 HQIEAGSKKTITCDPVDKDLTDSEMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTR 120 +IE S +CDPV KD TD+EMAL A+ QPE I+L GV G+R DH LA++ ++ + Sbjct: 61 AEIERLSMHVSSCDPVMKDWTDTEMALTWAIEQQPEEIVLLGVLGSRFDHMLANVHLLNK 120 Query: 121 ALQRQVHCSVMDTHNYITLTGSQTEIHDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATL 180 ALQ +C ++D N I L Q+ I + ++SLLP TPEVTGITL GF YPL ATL Sbjct: 121 ALQTGSNCRILDETNEIRLIDRQSTIEQDHFDHISLLPFTPEVTGITLTGFLYPLKDATL 180 Query: 181 KLGQSLGVSNILTAERGTVSIESGLLLIIQSKD 213 ++G +LG+SN+LT + GT++I++G LL+++SKD Sbjct: 181 RIGDTLGISNLLTEQTGTITIQTGKLLVVKSKD 213 >A4J9F1.1 A4J9F1_DESRM Thiamine pyrophosphokinase Length = 213 Score = 219 bits (557), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 108/213 (50%), Positives = 146/213 (68%) Query: 1 MPTKRVVIFAGGDLSAEYLSLLDEEDFVIGADRGALFLITHGVEPDISVGDFDSVPPEAL 60 M R +IF+GG+L + L + DF++G DRGA FLI +G+ PD+++GDFDSV L Sbjct: 1 MTQNRTLIFSGGNLGSWALKKIAPGDFLLGVDRGAYFLIENGISPDMAIGDFDSVTHSQL 60 Query: 61 HQIEAGSKKTITCDPVDKDLTDSEMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTR 120 +I+ SK+ ++CDPV KD TD+EMA + AL +P I+L GV G R DH+LA+I ++ + Sbjct: 61 ARIKEESKEYLSCDPVQKDETDTEMAFNWALQRKPGEIILLGVLGDRFDHSLANIHLLAK 120 Query: 121 ALQRQVHCSVMDTHNYITLTGSQTEIHDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATL 180 ALQ V C ++D N I L Q I +T +SLLPLT +VTGITL GFQYPL ATL Sbjct: 121 ALQEGVPCCIVDEKNEIFLIKDQAIIKKDQFTQISLLPLTQQVTGITLTGFQYPLYNATL 180 Query: 181 KLGQSLGVSNILTAERGTVSIESGLLLIIQSKD 213 +G SLG+SN+L+ E G + + SGLLLII+SKD Sbjct: 181 VVGHSLGISNVLSGETGKIELASGLLLIIKSKD 213 >B1DLR5.1 B1DLR5_9BACL Thiamine pyrophosphokinase Length = 213 Score = 196 bits (498), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 99/212 (46%), Positives = 140/212 (66%), Gaps = 1/212 (0%) Query: 3 TKRVVIFAGGDLSAEYLSLLDEEDFVIGADRGALFLITHGVEPDISVGDFDSVPPEALHQ 62 R++I GG L + D +IGADRGALFL+ HG PD+++GDFDSV E L Sbjct: 2 NNRIIICTGGRLGEWVRPYIRPGDRLIGADRGALFLVQHGYTPDLALGDFDSVTLEELEL 61 Query: 63 IEAGSKKTITCDPVDKDLTDSEMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTRAL 122 I + S++T+T DP+DKD TD+E+A AL P I + G G+R DH+LA++ ++ A+ Sbjct: 62 IRSVSRETVTYDPIDKDYTDTELAFRYALEQSPSEIRMLGALGSRFDHSLANVHLLKLAI 121 Query: 123 QRQVHCSVMDTHNYITLTGSQTE-IHDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATLK 181 ++ + S+ D HN I+L T + Y+++SLLPL+ EV GITL GF YPL ATL+ Sbjct: 122 EQGIPASITDEHNTISLAEEGTTGLQQSEYSHISLLPLSEEVKGITLRGFVYPLHEATLR 181 Query: 182 LGQSLGVSNILTAERGTVSIESGLLLIIQSKD 213 +GQSLG+SNIL GT+++ +G LL+IQSKD Sbjct: 182 IGQSLGISNILAEPDGTLTVRAGTLLVIQSKD 213 >Q8ER19.1 Q8ER19_OCEIH Hypothetical conserved protein Length = 216 Score = 171 bits (434), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 88/191 (46%), Positives = 126/191 (65%), Gaps = 2/191 (1%) Query: 24 EEDFVIGADRGALFLITHGVEPDISVGDFDSVPPEALHQIEAGSKKTITCDPVDKDLTDS 83 E DF IGADRGAL+L + DI++GDFDSV E +I +KK +T +K+ TD Sbjct: 24 EVDFWIGADRGALYLAESNLNIDIALGDFDSVSDEEKQKINKHAKKVLTY-STEKNYTDL 82 Query: 84 EMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTRALQRQVHCSVMDTHNYITLTGS- 142 E+A+ +A Q + ILLFGVTG R+DH L +IQ++ R LQ+ V ++D N I + S Sbjct: 83 ELAIQIASDLQVDRILLFGVTGGRLDHELMNIQLLYRLLQKNVEAKIIDHSNQIEMVESG 142 Query: 143 QTEIHDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATLKLGQSLGVSNILTAERGTVSIE 202 + I++ GYTY+S + T +V G+TLEGF+YPL T++ G SL +SN LT ++GT S Sbjct: 143 KYTINNQGYTYISFISFTNKVEGLTLEGFKYPLIDQTIEWGSSLCISNKLTQKKGTFSFR 202 Query: 203 SGLLLIIQSKD 213 +G+LL+I+S D Sbjct: 203 AGILLLIKSCD 213 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,227,673,734 Number of extensions: 49816802 Number of successful extensions: 119518 Number of sequences better than 1.0e-05: 494 Number of HSP's gapped: 118825 Number of HSP's successfully gapped: 494 Length of query: 213 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 83 Effective length of database: 1,835,964,253 Effective search space: 152385032999 Effective search space used: 152385032999 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)