BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0042.p (257 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9BPX8.1 A9BPX8_DELAS Two component heavy metal response transcr... 120 2e-25 B2UF45.1 B2UF45_RALPJ Two component heavy metal response transcr... 119 2e-25 A7CLA3.1 A7CLA3_BURPI Two component heavy metal response transcr... 118 4e-25 B8IP07.1 B8IP07_METNO Two component transcriptional regulator, w... 118 6e-25 B0UGM4.1 B0UGM4_METS4 Two component transcriptional regulator, w... 117 8e-25 >A9BPX8.1 A9BPX8_DELAS Two component heavy metal response transcriptional regulator, winged helix family Length = 230 Score = 120 bits (300), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 68/168 (40%), Positives = 87/168 (51%), Gaps = 1/168 (0%) Query: 81 GWSLVSDLREQGKRLTVMVLSDGRYXXXXXXXXXXXXNEYMVKPVHTGEFKCRVDNLMXX 140 GW +V LR+ GK++ V+ L+ ++Y+VKP E RV L+ Sbjct: 59 GWRIVRSLRDAGKQVPVLFLTARGSVDDRVKGLELGADDYLVKPFAFSELLARVRTLLRR 118 Query: 141 XXXXXXXXXXXKVDGLVLDPTRRLVQRDGNELKMTPKEFDLLYYLAMHLGEVCPREEILK 200 D LVLD RR R G + +T KEF LL LA GEV PR I Sbjct: 119 GSAPSQPDRIQVAD-LVLDLPRRRATRAGQRINLTSKEFALLELLARRQGEVLPRSLIAS 177 Query: 201 QVWDYHFHPDTNVVDVYIRHIRLKVDKGHKNKLIHTVRGIGYVLRAPE 248 QVWD +F D+NV+DV IR +R K+D KLIHTVRG+GY L AP+ Sbjct: 178 QVWDMNFDSDSNVIDVAIRRLRAKIDDAFNPKLIHTVRGMGYTLDAPD 225 >B2UF45.1 B2UF45_RALPJ Two component heavy metal response transcriptional regulator, winged helix family Length = 228 Score = 119 bits (299), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 79/212 (37%), Positives = 98/212 (46%), Gaps = 2/212 (0%) Query: 37 LQQALQELGVQTAIXXXXXXXXXXXXXVEPALLIADLNHAAAWAGWSLVSDLREQGKRLT 96 LQQ L E G + + LLI D+ GW ++ LR + Sbjct: 16 LQQGLSEAGFVVDLARNGLDGRHLAMTGDYDLLILDV-MLPDLEGWQILHALRSTELAVP 74 Query: 97 VMVLSDGRYXXXXXXXXXXXXNEYMVKPVHTGEFKCRVDNLMXXXXXXXXXXXXXKVDGL 156 V+ L+ ++YMVKP E RV L+ D L Sbjct: 75 VLFLTARDSVADRVKGLELGADDYMVKPFAFAELLARVRTLLRRGSAPSLAERIQVAD-L 133 Query: 157 VLDPTRRLVQRDGNELKMTPKEFDLLYYLAMHLGEVCPREEILKQVWDYHFHPDTNVVDV 216 VLD TRR R G + +T KEF LL LA GEV PR I QVWD +F DTNV+DV Sbjct: 134 VLDLTRRRASRGGQRITLTSKEFSLLELLARRHGEVLPRSLIASQVWDMNFDSDTNVIDV 193 Query: 217 YIRHIRLKVDKGHKNKLIHTVRGIGYVLRAPE 248 IR +R K+D + KLIHTVRG+GYVL PE Sbjct: 194 AIRRLRAKIDDNFEPKLIHTVRGMGYVLEGPE 225 >A7CLA3.1 A7CLA3_BURPI Two component heavy metal response transcriptional regulator, winged helix family Length = 248 Score = 118 bits (296), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 78/212 (36%), Positives = 99/212 (46%), Gaps = 2/212 (0%) Query: 37 LQQALQELGVQTAIXXXXXXXXXXXXXVEPALLIADLNHAAAWAGWSLVSDLREQGKRLT 96 LQQ L E G + + LLI D+ GW ++ LR + Sbjct: 36 LQQGLSEAGFVVDLARNGMDGRHLAMTGDYDLLILDV-MLPDLEGWQILHALRSAELAVP 94 Query: 97 VMVLSDGRYXXXXXXXXXXXXNEYMVKPVHTGEFKCRVDNLMXXXXXXXXXXXXXKVDGL 156 V+ L+ ++Y+VKP E RV +L+ D L Sbjct: 95 VLFLTARDSVADRVKGLELGADDYLVKPFAFAELLARVRSLLRRGSAPSLAERIQVAD-L 153 Query: 157 VLDPTRRLVQRDGNELKMTPKEFDLLYYLAMHLGEVCPREEILKQVWDYHFHPDTNVVDV 216 VLD TRR R G + +T KEF LL LA GEV PR I QVWD +F DTNV+DV Sbjct: 154 VLDLTRRRASRGGQRIALTSKEFSLLELLARRHGEVLPRSLIASQVWDMNFDSDTNVIDV 213 Query: 217 YIRHIRLKVDKGHKNKLIHTVRGIGYVLRAPE 248 IR +R K+D + KLIHTVRG+GYVL PE Sbjct: 214 AIRRLRAKIDDNFEPKLIHTVRGMGYVLEGPE 245 >B8IP07.1 B8IP07_METNO Two component transcriptional regulator, winged helix family Length = 228 Score = 118 bits (295), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 65/166 (39%), Positives = 88/166 (53%) Query: 81 GWSLVSDLREQGKRLTVMVLSDGRYXXXXXXXXXXXXNEYMVKPVHTGEFKCRVDNLMXX 140 G SL+ LREQG V++LS ++Y+ KP E R++ L Sbjct: 59 GLSLIRSLREQGVETPVLILSALGQVDDRVKGLRAGGDDYLPKPYAFSELLARIEVLARR 118 Query: 141 XXXXXXXXXXXKVDGLVLDPTRRLVQRDGNELKMTPKEFDLLYYLAMHLGEVCPREEILK 200 +V L LD V R G E+ + P+EF LL YL H G+V R +L+ Sbjct: 119 RGGGAGEPTAYRVGDLELDRLSHRVTRGGQEIVLQPREFRLLEYLMRHAGQVVTRTMLLE 178 Query: 201 QVWDYHFHPDTNVVDVYIRHIRLKVDKGHKNKLIHTVRGIGYVLRA 246 VWDYHF P TNV+DV++ +R KVDKG + +IHTVRG GY++RA Sbjct: 179 HVWDYHFDPQTNVIDVHVSRLRAKVDKGFERPMIHTVRGAGYMVRA 224 >B0UGM4.1 B0UGM4_METS4 Two component transcriptional regulator, winged helix family Length = 228 Score = 117 bits (294), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 66/166 (39%), Positives = 87/166 (52%) Query: 81 GWSLVSDLREQGKRLTVMVLSDGRYXXXXXXXXXXXXNEYMVKPVHTGEFKCRVDNLMXX 140 G SL+ LREQG V++LS ++Y+ KP E RV+ L Sbjct: 59 GLSLIRSLREQGVETPVLILSALGQVDDRVKGLRAGGDDYVPKPYAFSELLARVEVLARR 118 Query: 141 XXXXXXXXXXXKVDGLVLDPTRRLVQRDGNELKMTPKEFDLLYYLAMHLGEVCPREEILK 200 +V L LD V R G E+ + P+EF LL YL H G+V R +L+ Sbjct: 119 RGGGAGEPTAYRVGDLELDRLSHRVTRGGQEIVLQPREFRLLEYLMRHAGQVVTRTMLLE 178 Query: 201 QVWDYHFHPDTNVVDVYIRHIRLKVDKGHKNKLIHTVRGIGYVLRA 246 VWDYHF P TNV+DV++ +R KVDKG +IHTVRG GY++RA Sbjct: 179 HVWDYHFDPQTNVIDVHVSRLRAKVDKGFDRPMIHTVRGAGYMVRA 224 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,303,824,050 Number of extensions: 45001801 Number of successful extensions: 119612 Number of sequences better than 1.0e-05: 9771 Number of HSP's gapped: 118319 Number of HSP's successfully gapped: 9771 Length of query: 257 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 124 Effective length of database: 1,807,701,208 Effective search space: 224154949792 Effective search space used: 224154949792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)