BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0041.p (356 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6P0.1 B3K6P0_9BACI UBA/THIF-type NAD/FAD binding protein 458 e-127 B7IY03.1 B7IY03_BACC2 HesA/moeB/thiF family protein 417 e-115 B5UJG1.1 B5UJG1_BACCE HesA/moeB/thiF family protein 416 e-114 Q3ELR9.1 Q3ELR9_BACTI Molybdopterin biosynthesis MoeB protein 416 e-114 A6CKN5.1 A6CKN5_9BACI Thiamine/molybdopterin biosynthesis ThiF/M... 416 e-114 >B3K6P0.1 B3K6P0_9BACI UBA/THIF-type NAD/FAD binding protein Length = 375 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 211/336 (62%), Positives = 254/336 (75%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 YSRQ RFTP G EGQ L S VL++G GALG+ ++ETL R G G++ + DRDYVEWSNL Sbjct: 39 YSRQERFTPLGKEGQIRLRNSRVLVLGAGALGSAVSETLVRAGIGQVTIVDRDYVEWSNL 98 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 QRQQL+TE+DA R+PKA AA RL INS++T+E V+DVRA EL L GVDL+MD T Sbjct: 99 QRQQLFTEQDAINRLPKAVAAAKRLSEINSDVTVEGVVMDVRAHELSVLCQGVDLMMDAT 158 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLLGAVPLGGDTCDTA 200 DNF TRLI+ND+ KHGIPWIYG CVGS G++FT LPGETPCLNCLLG +PLGGDTCD Sbjct: 159 DNFGTRLILNDIGFKHGIPWIYGGCVGSSGMSFTFLPGETPCLNCLLGMIPLGGDTCDIN 218 Query: 201 GILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPSCG 260 GILPQAVQ+V A+ T EALKLL G + LR K+LSFD+WRNE E GV+ AKK C +CG Sbjct: 219 GILPQAVQMVAAHQTMEALKLLSGNRQALRKKMLSFDLWRNEQLEFGVERAKKSDCSTCG 278 Query: 261 SHPVYPYLTAANTERSDVLCGRDTVQIRPAQRRQLNLQETAARLAGLGSGKVESNPYLVS 320 P YPYL+A + ++S+VLCGRDTVQIRP + QL L+E A RL L GK PY++ Sbjct: 279 EEPTYPYLSAEHADKSEVLCGRDTVQIRPGRPMQLQLEEMADRLNSLRQGKATVTPYMII 338 Query: 321 FTEEPYRMVVFGDGRALIHGTSDIAAARSFYHRYFG 356 F P+R+V+F DGR L+HGTSDIA ARS Y++Y G Sbjct: 339 FQASPHRLVIFQDGRTLVHGTSDIAKARSLYNQYVG 374 >B7IY03.1 B7IY03_BACC2 HesA/moeB/thiF family protein Length = 339 Score = 417 bits (1071), Expect = e-115, Method: Compositional matrix adjust. Identities = 191/336 (56%), Positives = 244/336 (72%), Gaps = 1/336 (0%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 YSRQ F+P G EGQ + + VLIIG GALG+ AE R G G+I + DRDYV+WSNL Sbjct: 5 YSRQELFSPIGEEGQQKIREKHVLIIGAGALGSANAEMFVRAGVGKITIVDRDYVDWSNL 64 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 QRQQLY E D + +PKA AAK RLE INS++TIEA V DV AEELE L+ VD+I+D T Sbjct: 65 QRQQLYAESDVKNNLPKAIAAKKRLEEINSDVTIEALVQDVTAEELEELVTNVDVIIDAT 124 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLLGAVPLGGDTCDTA 200 DNF+TR I+ND+AQK+ IPWIYGACVGSYG+++TILP +TPCL+CLL ++PLGG TCDTA Sbjct: 125 DNFETRFIVNDIAQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGATCDTA 184 Query: 201 GILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPSCG 260 GI+ AV LV ++ EALKLL E LR L+SFDVW+NE+ + V+ +K +CPSCG Sbjct: 185 GIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNCPSCG 244 Query: 261 SHPVYPYLTAANTERSDVLCGRDTVQIRPAQRRQLNLQETAARLAGLGSGKVESNPYLVS 320 + +YPYL NT ++ VLCGR+TVQIRP + +++ E +L + NPYL+S Sbjct: 245 ENAIYPYLNKENTSKTAVLCGRNTVQIRPPHKEEMDF-ERYKKLLDDRVNDLNVNPYLLS 303 Query: 321 FTEEPYRMVVFGDGRALIHGTSDIAAARSFYHRYFG 356 F+ E R+V F DGR L+HGT DI+ A++ YHRYFG Sbjct: 304 FSVEEKRLVAFKDGRVLVHGTKDISEAKTIYHRYFG 339 >B5UJG1.1 B5UJG1_BACCE HesA/moeB/thiF family protein Length = 339 Score = 416 bits (1069), Expect = e-114, Method: Compositional matrix adjust. Identities = 190/336 (56%), Positives = 245/336 (72%), Gaps = 1/336 (0%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 YSRQ F+P G EGQ + + VLIIG GALG+ AE R G G+I + DRDYV+WSNL Sbjct: 5 YSRQELFSPIGEEGQQKIREKHVLIIGAGALGSANAEMFVRAGVGKITIVDRDYVDWSNL 64 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 QRQQLY E D + +PKA AAK RLE INS++TIEA V DV AEELE L+ VD+I+D T Sbjct: 65 QRQQLYAESDVKNNLPKAIAAKKRLEEINSDVTIEALVQDVTAEELEELVTNVDVIIDAT 124 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLLGAVPLGGDTCDTA 200 DNF+TR I+ND+AQK+ IPWIYGACVGSYG+++TILP +TPCL+CLL ++PLGG TCDTA Sbjct: 125 DNFETRFIVNDIAQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGATCDTA 184 Query: 201 GILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPSCG 260 GI+ AV LV ++ EALKLL E LR L+SFD+W+NE+ + V+ ++ +CPSCG Sbjct: 185 GIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDMWKNEYSCMNVQKLRQHNCPSCG 244 Query: 261 SHPVYPYLTAANTERSDVLCGRDTVQIRPAQRRQLNLQETAARLAGLGSGKVESNPYLVS 320 + +YPYL NT ++ VLCGR+TVQIRP + ++N ++ L G + NPYL+S Sbjct: 245 ENALYPYLNKENTSKTAVLCGRNTVQIRPPHKEEMNFEQYKELLEG-RVNDLNINPYLLS 303 Query: 321 FTEEPYRMVVFGDGRALIHGTSDIAAARSFYHRYFG 356 F+ E R+V F DGR L+HGT DI+ A++ YHRYFG Sbjct: 304 FSIEDKRLVAFKDGRVLVHGTKDISEAKTIYHRYFG 339 >Q3ELR9.1 Q3ELR9_BACTI Molybdopterin biosynthesis MoeB protein Length = 339 Score = 416 bits (1069), Expect = e-114, Method: Compositional matrix adjust. Identities = 190/336 (56%), Positives = 246/336 (73%), Gaps = 1/336 (0%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 YSRQ F+P GAEGQ + + VLIIG GALG+ AE R G G+I + DRDYV+WSNL Sbjct: 5 YSRQELFSPIGAEGQQKIREKHVLIIGAGALGSANAEMFVRAGVGKITIVDRDYVDWSNL 64 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 QRQQLY E D + +PKA AAK RLE INS++TI+A V DV AEELE L+ VD+I+D T Sbjct: 65 QRQQLYAESDVKNNLPKAIAAKKRLEEINSDVTIKALVQDVTAEELEELVTNVDVIIDAT 124 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLLGAVPLGGDTCDTA 200 DNF+TR I+ND+AQK+ IPWIYGACVGSYG+++TILP +TPCL+CLL ++PLGG TCDTA Sbjct: 125 DNFETRFIVNDIAQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGATCDTA 184 Query: 201 GILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPSCG 260 GI+ AV LV ++ EALKLL E LR L+SFD+W+NE+ + V+ ++ +CPSCG Sbjct: 185 GIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDMWKNEYLCMNVQKLRQHNCPSCG 244 Query: 261 SHPVYPYLTAANTERSDVLCGRDTVQIRPAQRRQLNLQETAARLAGLGSGKVESNPYLVS 320 + +YPYL NT ++ VLCGR+TVQIRP + ++N ++ L G + NPYL+S Sbjct: 245 ENALYPYLNKENTSKTAVLCGRNTVQIRPPHKEEINFEQYKELLEG-RVNDLNVNPYLLS 303 Query: 321 FTEEPYRMVVFGDGRALIHGTSDIAAARSFYHRYFG 356 F+ E R+V F DGR L+HGT DI+ A++ YHRYFG Sbjct: 304 FSVEDKRLVAFKDGRVLVHGTKDISEAKTIYHRYFG 339 >A6CKN5.1 A6CKN5_9BACI Thiamine/molybdopterin biosynthesis ThiF/MoeB-like protein Length = 339 Score = 416 bits (1068), Expect = e-114, Method: Compositional matrix adjust. Identities = 197/336 (58%), Positives = 247/336 (73%), Gaps = 1/336 (0%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 YSRQ F+ G EGQ L++ VLI+G GALG+G AE L R G G++ + DRDYVEWSNL Sbjct: 5 YSRQELFSKIGTEGQRKLSEKHVLIVGAGALGSGNAEALVRAGVGKVTIIDRDYVEWSNL 64 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 QRQQLYTE+DA +R+PKAAAA+ RL INS ITIEA V D +ELE+L VD+I+D T Sbjct: 65 QRQQLYTEQDAAERIPKAAAAQKRLRQINSSITIEAIVADAGVQELENLHRDVDVILDAT 124 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLLGAVPLGGDTCDTA 200 DNFDTR+IIND++QK IPWIYGACVGSYGI +TI+PGETPCL+CLL +VPLGG TCDTA Sbjct: 125 DNFDTRMIINDISQKFSIPWIYGACVGSYGICYTIVPGETPCLHCLLESVPLGGATCDTA 184 Query: 201 GILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPSCG 260 GI+ AV +V + T E LKLL G +++R KL++FD+W+N+ I + KK C SCG Sbjct: 185 GIISPAVGMVVSYQTVEVLKLLVGDHDKIRKKLITFDLWQNQQSAISAASLKKPGCLSCG 244 Query: 261 SHPVYPYLTAANTERSDVLCGRDTVQIRPAQRRQLNLQETAARLAGLGSGKVESNPYLVS 320 S P YP+L+ N ++ VLCGR+TVQIRP + + NL++ A LA G GKVE NP+L+S Sbjct: 245 SKPTYPFLSYENQTKAAVLCGRNTVQIRPQTKGERNLEKLADMLAKTG-GKVERNPFLLS 303 Query: 321 FTEEPYRMVVFGDGRALIHGTSDIAAARSFYHRYFG 356 + +R+VVF DGR LIHG DI A+ YHRY G Sbjct: 304 YETSNHRLVVFKDGRVLIHGIKDITEAKKVYHRYLG 339 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,982,103,893 Number of extensions: 80656565 Number of successful extensions: 264751 Number of sequences better than 1.0e-05: 2812 Number of HSP's gapped: 260917 Number of HSP's successfully gapped: 2819 Length of query: 356 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 219 Effective length of database: 1,770,017,148 Effective search space: 387633755412 Effective search space used: 387633755412 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)