BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0034.p (223 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6Q7.1 B3K6Q7_9BACI Apurinic endonuclease Apn1 271 3e-71 C0ZBK5.1 C0ZBK5_BREBN Putative endonuclease IV EC=3.1.21.2 222 2e-56 Q67S99.1 END4_SYMTH Probable endonuclease 4 EC=3.1.21.2 172 2e-41 B1DLQ3.1 B1DLQ3_9BACL AP endonuclease family 2 140 6e-32 C0GKM0.1 C0GKM0_9FIRM Apurinic endonuclease Apn1 EC=3.1.21.2 118 4e-25 >B3K6Q7.1 B3K6Q7_9BACI Apurinic endonuclease Apn1 Length = 282 Score = 271 bits (693), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 132/217 (60%), Positives = 154/217 (70%), Gaps = 1/217 (0%) Query: 1 VSIAHTPYPTNLAAA-SEGATPRPVMVASLLNDLEIAEACGSLGIVVHFGHFSGTEPLQG 59 VSIAHTPYPTN+A + G + V SL NDL IAEACGSLGIVVHFGH L+G Sbjct: 65 VSIAHTPYPTNMAVGLTRGEAFYQLTVDSLKNDLMIAEACGSLGIVVHFGHLKSENVLEG 124 Query: 60 YQNIIQCINDTLKGWDGQAKLLIENQAGNHGKEGMTLEELVKIRELSRYPEKIGFCLDTC 119 YQNII+C+ND L W G+AKLLIENQAG+HG GMTLEE VK+R+L+ YPEKIGFCLDTC Sbjct: 125 YQNIIRCMNDVLSSWTGRAKLLIENQAGDHGSMGMTLEESVKVRQLADYPEKIGFCLDTC 184 Query: 120 HAFSAGIWNPQHTGEFLQRGESLGYWPHLTAVHLNDSMYAFGLRKDRHAQIGKGCIGDAG 179 H F+AG W+ +T E L RG+ L YW AVHLNDS Y G RKDRHA+IG+G IG Sbjct: 185 HLFAAGGWDLSNTDELLARGKELAYWESAVAVHLNDSKYGAGSRKDRHARIGQGMIGTEA 244 Query: 180 LRTLLTAKPFGRTAIALETGKGPDGTHRQEIAAVRKW 216 L+ L+ A A LE+ KG DGTHR++IA V W Sbjct: 245 LKRLVLAPELAGKAFVLESEKGADGTHREDIAKVLSW 281 >C0ZBK5.1 C0ZBK5_BREBN Putative endonuclease IV EC=3.1.21.2 Length = 280 Score = 222 bits (566), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 112/214 (52%), Positives = 140/214 (65%), Gaps = 2/214 (0%) Query: 1 VSIAHTPYPTNLAAASEGATPRPVMVASLLNDLEIAEACGSLGIVVHFGHFSGTEPLQGY 60 +SIAHTPYP NL E V V S+ NDLEIAEACG+LG+VVHFG + G + L GY Sbjct: 61 LSIAHTPYPVNLCV--EEQELFAVTVGSVRNDLEIAEACGALGVVVHFGQYKGADVLAGY 118 Query: 61 QNIIQCINDTLKGWDGQAKLLIENQAGNHGKEGMTLEELVKIRELSRYPEKIGFCLDTCH 120 + +IQ +N L GW+G+A+LLIEN AG + G TLEEL ++R+L P+K+GFCLDTCH Sbjct: 119 KFMIQMVNQILDGWNGKAQLLIENNAGQGNRMGTTLEELTQVRQLFAEPQKVGFCLDTCH 178 Query: 121 AFSAGIWNPQHTGEFLQRGESLGYWPHLTAVHLNDSMYAFGLRKDRHAQIGKGCIGDAGL 180 AF++G+W GE R L Y+ L AVHLNDS+Y G +DRHA IGKG IGDA + Sbjct: 179 AFASGLWKGNDWGEVADRMRELEYFTSLRAVHLNDSVYPSGSFRDRHASIGKGMIGDAAI 238 Query: 181 RTLLTAKPFGRTAIALETGKGPDGTHRQEIAAVR 214 T L I LET +G +G H +EI VR Sbjct: 239 ATFLQTPELQGLPIVLETARGSEGGHGEEIKHVR 272 >Q67S99.1 END4_SYMTH Probable endonuclease 4 EC=3.1.21.2 Length = 276 Score = 172 bits (437), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 93/218 (42%), Positives = 128/218 (58%), Gaps = 10/218 (4%) Query: 1 VSIAHTPYPTNLAAASEGATPRP----VMVASLLNDLEIAEACGSLGIVVHFGHFSGTEP 56 V++AH PY TNL+ TP P + +ASL DLE AEA G++G V H G G Sbjct: 61 VAVAHAPYITNLS------TPDPELQAISIASLKQDLENAEAYGAIGCVCHMGKHVGEGE 114 Query: 57 LQGYQNIIQCINDTLKGWDGQAKLLIENQAGNHGKEGMTLEELVKIRELSRYPEKIGFCL 116 G +++ +N L+ + G LL+EN AG + G LEEL+++R PE+I FC Sbjct: 115 AYGRARMVETLNRLLEAYTGSCPLLLENTAGMGSELGTHLEELMEVRSRVEQPERIAFCF 174 Query: 117 DTCHAFSAGIWNPQHTGEFLQRGESLGYWPHLTAVHLNDSMYAFGLRKDRHAQIGKGCIG 176 DTCHAF+AGI+ P+ +F+ ++GYW L AVHLNDS + G RKDRHA +GKG +G Sbjct: 175 DTCHAFAAGIYRPEDWEDFVAHARAIGYWGLLRAVHLNDSKFDHGSRKDRHANLGKGFLG 234 Query: 177 DAGLRTLLTAKPFGRTAIALETGKGPDGTHRQEIAAVR 214 +AG+ TLL + F + LET + + EIA R Sbjct: 235 EAGIATLLRSGAFEGLPVVLETPVKDEAEYGPEIAYAR 272 >B1DLQ3.1 B1DLQ3_9BACL AP endonuclease family 2 Length = 149 Score = 140 bits (354), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 66/143 (46%), Positives = 94/143 (65%) Query: 74 WDGQAKLLIENQAGNHGKEGMTLEELVKIRELSRYPEKIGFCLDTCHAFSAGIWNPQHTG 133 W+G AKLLIE Q+G H G T+EEL +IR L P K+ FCLDTCH F++G+W+ + Sbjct: 5 WNGNAKLLIEIQSGEHTFMGTTIEELAQIRGLCAEPAKLAFCLDTCHMFASGLWHGETDA 64 Query: 134 EFLQRGESLGYWPHLTAVHLNDSMYAFGLRKDRHAQIGKGCIGDAGLRTLLTAKPFGRTA 193 E+L++ SLG H+ AVH NDSM+A G R+DRHA +G G IG+AGL+ L++ Sbjct: 65 EWLKKAVSLGVLEHVAAVHFNDSMFAAGARRDRHAALGSGHIGEAGLKWLMSVPALAGVP 124 Query: 194 IALETGKGPDGTHRQEIAAVRKW 216 LET DG++ +++ +R+W Sbjct: 125 FILETPSDEDGSYGRQLNLMREW 147 >C0GKM0.1 C0GKM0_9FIRM Apurinic endonuclease Apn1 EC=3.1.21.2 Length = 277 Score = 118 bits (295), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 76/224 (33%), Positives = 117/224 (52%), Gaps = 21/224 (9%) Query: 3 IAHTPYPTNLAAASEGATPRPVMVASLLNDLEIAEACGSLGIVVHFGHFSGTEPLQGYQN 62 + H PY N+A+ +E A MV + +DLE +A G+ +V+H G +G+ G + Sbjct: 64 MGHLPYTVNMASPAERAYNFAKMVVA--DDLERMDAVGAEYLVIHPGSHAGSGREAGLER 121 Query: 63 IIQCINDTLKGWDGQAKLLIENQAGNHGKEGMTLEELVKIRELSRYPEKIGFCLDTCHAF 122 I+ C+ ++ +DG+ LL+E AG G E TL ++ +I E +P+ +G CLD+CH Sbjct: 122 IVACLEESFLPYDGETSLLLETMAGQ-GSEIGTLADIAEIMEKLSWPDGMGVCLDSCHLT 180 Query: 123 SAGIWNPQHTGEFLQRGE----------SLGYWPHLTAVHLNDSMYAFGLRKDRHAQIGK 172 AG +FLQ+ E ++G + A+HLNDS + G KDRH +IG Sbjct: 181 GAGY-------DFLQKEEVDRLVTDVKKTVGL-SRVRAMHLNDSKFPPGTHKDRHERIGA 232 Query: 173 GCIGDAGLRTLLTAKPFGRTAIALETGKGPDGTHRQEIAAVRKW 216 G +G GL L+T F +ALET + +EI +R W Sbjct: 233 GYLGKEGLLNLITHPAFKDLPMALETPVEDFQQYGEEITLIRSW 276 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,409,256,467 Number of extensions: 57372727 Number of successful extensions: 117774 Number of sequences better than 1.0e-05: 801 Number of HSP's gapped: 116894 Number of HSP's successfully gapped: 801 Length of query: 223 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 92 Effective length of database: 1,826,543,238 Effective search space: 168041977896 Effective search space used: 168041977896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)