BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0032.p (271 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6Q8.1 B3K6Q8_9BACI DNA glycosylase EC=4.2.99.18 340 6e-92 B1DLQ1.1 B1DLQ1_9BACL DNA glycosylase EC=4.2.99.18 280 8e-74 Q2B3G7.1 Q2B3G7_9BACI DNA glycosylase EC=4.2.99.18 257 7e-67 A6CKK3.1 A6CKK3_9BACI DNA glycosylase EC=4.2.99.18 253 1e-65 C0Z883.1 C0Z883_BREBN Putative formamidopyrimidine-DNA glycosyla... 251 4e-65 >B3K6Q8.1 B3K6Q8_9BACI DNA glycosylase EC=4.2.99.18 Length = 273 Score = 340 bits (873), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 162/272 (59%), Positives = 202/272 (74%), Gaps = 2/272 (0%) Query: 1 MPELPEMENYRKLLSQHLINVPITGVTVNREKTINMETPEFTQALLGARIVFVERRAKHI 60 MPELPEMENYR+LLSQ ++NVPIT V VNREK++N+ EFT AL G++++FVERR KH+ Sbjct: 1 MPELPEMENYRRLLSQSILNVPITDVVVNREKSLNVTIDEFTSALKGSKVIFVERRGKHL 60 Query: 61 XXXXXXXXXXXXXXXXXXXXXYG--TEAERPERSTQVELAFGDHILYFMGLRLGYLHLLS 118 G + ERP RSTQVE+ FGD +LYF+GLRLGYLH L+ Sbjct: 61 IFHLHNGGRLLLHLMLGGILYLGRRDDEERPSRSTQVEITFGDQVLYFIGLRLGYLHFLT 120 Query: 119 VKEGEAAMGKLGPELLDRRMTPERFAGLLKGRRGALKSLMVNQQVMAGIGNCYADEIAFE 178 +E E A+ LGPELLDRRMT RF L+ RRGALK+ +VNQQV+AGIGNCYADEIAF+ Sbjct: 121 ARETENALKDLGPELLDRRMTKARFVELIGKRRGALKTTLVNQQVVAGIGNCYADEIAFD 180 Query: 179 AGLLPTTLVQNLTPEAVARLYDSVRKVLTEATETGGYMEMPFMTGDTVTGSYNDKCKVYD 238 A + P + +Q+L E++ +LYDS KV+ EAT+ GGYMEMPF GD +TG+YND+CKVYD Sbjct: 181 ARIHPLSKIQDLPEESLEQLYDSTVKVMHEATDGGGYMEMPFQEGDVLTGAYNDECKVYD 240 Query: 239 REGEPCLRCGGTIVKIELSGRKVFYCPDCQHD 270 REGEPC+RCG IVK E + RKVF+CP+CQH+ Sbjct: 241 REGEPCVRCGTPIVKAEHASRKVFFCPNCQHE 272 >B1DLQ1.1 B1DLQ1_9BACL DNA glycosylase EC=4.2.99.18 Length = 271 Score = 280 bits (717), Expect = 8e-74, Method: Compositional matrix adjust. Identities = 133/271 (49%), Positives = 173/271 (63%) Query: 1 MPELPEMENYRKLLSQHLINVPITGVTVNREKTINMETPEFTQALLGARIVFVERRAKHI 60 MPELPEME+YR LS+ +I PITG V R+K+IN+ +F L+G I FVERR KH+ Sbjct: 1 MPELPEMEHYRLRLSELIIGSPITGTEVTRDKSINISAEQFEAELVGRTIWFVERRGKHL 60 Query: 61 XXXXXXXXXXXXXXXXXXXXXYGTEAERPERSTQVELAFGDHILYFMGLRLGYLHLLSVK 120 +G+E ERP+R+ QV + F LYF+GLRLGYLH +SVK Sbjct: 61 LFHLDNGKRLVLHLMLGGTLFFGSEEERPDRTVQVTIRFATGNLYFIGLRLGYLHFMSVK 120 Query: 121 EGEAAMGKLGPELLDRRMTPERFAGLLKGRRGALKSLMVNQQVMAGIGNCYADEIAFEAG 180 E +A + +LGP+ D+R+T ERF +RGALK+ +V+Q V++GIGNCYADEIAF A Sbjct: 121 EADAKLSELGPDPFDKRLTLERFKARFNKKRGALKTALVDQHVLSGIGNCYADEIAFAAK 180 Query: 181 LLPTTLVQNLTPEAVARLYDSVRKVLTEATETGGYMEMPFMTGDTVTGSYNDKCKVYDRE 240 + P + +LT E R+Y+S+ VL EA GGYME P GD +TG YND C+VYDR Sbjct: 181 IRPDAKIPSLTDETWERVYESMHSVLKEAISKGGYMEQPLTAGDAITGGYNDHCQVYDRG 240 Query: 241 GEPCLRCGGTIVKIELSGRKVFYCPDCQHDE 271 GEPC CG I + E+S RK F CP CQ ++ Sbjct: 241 GEPCFVCGTAIEQFEISSRKAFVCPVCQKEQ 271 >Q2B3G7.1 Q2B3G7_9BACI DNA glycosylase EC=4.2.99.18 Length = 275 Score = 257 bits (657), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 126/268 (47%), Positives = 169/268 (63%) Query: 1 MPELPEMENYRKLLSQHLINVPITGVTVNREKTINMETPEFTQALLGARIVFVERRAKHI 60 MPELPEME R+LL + L + IT +NREK+IN+ EF + + + I ++RRAKH+ Sbjct: 7 MPELPEMETCRQLLGRQLTGLMITEAEINREKSINVPPAEFIRQVQNSTITSIDRRAKHL 66 Query: 61 XXXXXXXXXXXXXXXXXXXXXYGTEAERPERSTQVELAFGDHILYFMGLRLGYLHLLSVK 120 G + + P+R+ QV L+FG LYF+GLRLGYLHLL+ + Sbjct: 67 IFRLSSGKNLLLHLMLGGWMYLGNDEDNPDRTKQVILSFGSRKLYFIGLRLGYLHLLTAQ 126 Query: 121 EGEAAMGKLGPELLDRRMTPERFAGLLKGRRGALKSLMVNQQVMAGIGNCYADEIAFEAG 180 E E +LGPE LD T FA LL+ +RG LK+ +VNQ +AGIGNCY+DEI +AG Sbjct: 127 ELEKEFEELGPEPLDPGFTAASFAQLLEKKRGMLKTSLVNQHFIAGIGNCYSDEICHQAG 186 Query: 181 LLPTTLVQNLTPEAVARLYDSVRKVLTEATETGGYMEMPFMTGDTVTGSYNDKCKVYDRE 240 LLP ++ L+ +RLY +++ V A ++GGYM+ P GD +TG YN++C VYDR+ Sbjct: 187 LLPMRKIEELSEMEKSRLYPAMKTVFANAIQSGGYMDEPLFKGDLITGGYNERCMVYDRK 246 Query: 241 GEPCLRCGGTIVKIELSGRKVFYCPDCQ 268 GE CLRCG IVK E+S RK FYCP CQ Sbjct: 247 GESCLRCGSQIVKEEISSRKTFYCPGCQ 274 >A6CKK3.1 A6CKK3_9BACI DNA glycosylase EC=4.2.99.18 Length = 270 Score = 253 bits (646), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 122/269 (45%), Positives = 168/269 (62%), Gaps = 1/269 (0%) Query: 1 MPELPEMENYRKLLSQHLINVPITGVTVNREKTINMETPEFTQALLGARIVFVERRAKHI 60 MPELPEMENY+ LLS+ ++N I+ V + REK+IN+ EFT + I V RRAKH+ Sbjct: 1 MPELPEMENYKNLLSERILNKQISEVEIGREKSINVPVDEFTHLVKNRTIKKVTRRAKHL 60 Query: 61 XXXXXXXXXXXXXXXXXXXXXYGTEAERPERSTQVELAFGDHILYFMGLRLGYLHLL-SV 119 YG+E E+P+R+ Q+ L+FG LYF+GLRLGYLHL + Sbjct: 61 LFHLDNDVVLLLHLMLGGWMFYGSEQEKPDRTIQIRLSFGIQHLYFIGLRLGYLHLYKDL 120 Query: 120 KEGEAAMGKLGPELLDRRMTPERFAGLLKGRRGALKSLMVNQQVMAGIGNCYADEIAFEA 179 E + LGPE + + E+F L RRG+LK+ +VNQ+ +AGIGNCY+DEI F A Sbjct: 121 DEAAKELDDLGPEPITPPFSVEQFLDLAGKRRGSLKTTLVNQRFLAGIGNCYSDEICFHA 180 Query: 180 GLLPTTLVQNLTPEAVARLYDSVRKVLTEATETGGYMEMPFMTGDTVTGSYNDKCKVYDR 239 ++P L+ +L+ S++ +L +A +GGYME P GD +TG YN KC+VYDR Sbjct: 181 AIMPGRKFNELSQHETRQLFQSMQFILNDAIASGGYMEEPLFPGDNLTGGYNSKCRVYDR 240 Query: 240 EGEPCLRCGGTIVKIELSGRKVFYCPDCQ 268 EGEPC+RCG I+K ++S +K FYC +CQ Sbjct: 241 EGEPCIRCGSQIIKDKISSKKTFYCGNCQ 269 >C0Z883.1 C0Z883_BREBN Putative formamidopyrimidine-DNA glycosylase EC=3.2.2.23 Length = 268 Score = 251 bits (642), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 129/269 (47%), Positives = 167/269 (62%), Gaps = 1/269 (0%) Query: 1 MPELPEMENYRKLLSQHLINVPITGVTVNREKTINMETPEFTQALLGARIVFVERRAKHI 60 MPELPEMENYR+LL + + IT V REKTIN+ EF + L R+ V+RR KH+ Sbjct: 1 MPELPEMENYRRLLQEKIGGGTITATHVQREKTINLPPAEFARLLQDNRLTLVDRRGKHL 60 Query: 61 XXXXXXXXXXXXXXXXXXXXXYGTEAERPERSTQVELAFGDHILYFMGLRLGYLHLLSVK 120 G+ ++ +R+ QV L+FG+ +LYF GLRLGYLHLL+ Sbjct: 61 LFHLESGHVLLLHLMLGGFLYLGSAEDKLKRTAQVTLSFGERLLYFHGLRLGYLHLLTNV 120 Query: 121 EGEAAMGKLGPELLDRRMTPERFAGLLKGRRGALKSLMVNQQVMAGIGNCYADEIAFEAG 180 + + + LGPE LD T RF LL +R LK+ +VNQ +AGIGNCYADEI F A Sbjct: 121 QIDERLAPLGPEPLDPLFTFTRFTELLADKRSVLKTALVNQHWLAGIGNCYADEICFHAA 180 Query: 181 LLPTTLVQNLTPEAVARLYDSVRKVLTEATETGGYMEMPFMTGDTVTGSYNDKCKVYDRE 240 +LPT + L+ E RLY S++ VLTEA GGYM+ P GD++TG ++++C+VYDR Sbjct: 181 ILPTRTIPTLSLEEQKRLYHSMQTVLTEAIRFGGYMQ-PLFQGDSLTGGFDERCQVYDRG 239 Query: 241 GEPCLRCGGTIVKIELSGRKVFYCPDCQH 269 GEPC RCG I K ELS RKVF C +CQH Sbjct: 240 GEPCPRCGQPIEKSELSSRKVFACANCQH 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,468,488,830 Number of extensions: 54627278 Number of successful extensions: 129212 Number of sequences better than 1.0e-05: 1551 Number of HSP's gapped: 127708 Number of HSP's successfully gapped: 1551 Length of query: 271 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 138 Effective length of database: 1,807,701,208 Effective search space: 249462766704 Effective search space used: 249462766704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)