BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0031.p (275 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6Q9.1 B3K6Q9_9BACI HAD-superfamily subfamily IIA hydrolase li... 334 5e-90 B1DLQ0.1 B1DLQ0_9BACL HAD-superfamily subfamily IIA hydrolase li... 259 2e-67 C2PM83.1 C2PM83_BACCE Putative uncharacterized protein 226 2e-57 B5V7K6.1 B5V7K6_BACCE Phosphatase,haloacid dehalogenase family 226 2e-57 Q81XP1.1 Q81XP1_BACAN Phosphatase,haloacid dehalogenase family 225 3e-57 >B3K6Q9.1 B3K6Q9_9BACI HAD-superfamily subfamily IIA hydrolase like protein Length = 262 Score = 334 bits (856), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 163/260 (62%), Positives = 200/260 (76%) Query: 14 MDHVGGLLIDLDGTLYHGGRMIQGADLLVAALRTAGIPFMFVTNNSSRTPASVAAHLCAM 73 M+ GLLIDLDGTLYHG I+GADLL+ L+ IPF++VTNNSSRTP VAAHL M Sbjct: 1 MEKPKGLLIDLDGTLYHGRNRIEGADLLIERLKEMQIPFLYVTNNSSRTPEQVAAHLMEM 60 Query: 74 GIEAEAQEVCTSSLAAARYIAGESPGATVAILGEEGLIQACREAGLRLVSEAPEYVVQGI 133 GI A +EVCTSSLAAA+YIA ESPGA VA+LGEEGL +A AGL +V ++PEYV+QGI Sbjct: 61 GIPALPEEVCTSSLAAAKYIAEESPGAKVAMLGEEGLREALLSAGLTIVEQSPEYVIQGI 120 Query: 134 DRSFTYDSLAMASRWVREGAKFVLTNPDLMLPSDDGVMPGAGSLGAAIEAASGIPPVVIG 193 DRSF YD L A RW++EGA +LTNPDL LPSD G+MPGAGSLGA+IEAASG+ P VIG Sbjct: 121 DRSFDYDKLTRAVRWIQEGAVSILTNPDLQLPSDTGLMPGAGSLGASIEAASGVKPTVIG 180 Query: 194 KPEAHLIAYATSLMGIDPGEAVVVGDNMRTDISAGARAGCRTILVLTGLTTRDNLEDYQR 253 KP + L+ YA+ +G+ P E V+GDN+RTDI+AG AGC+T+LV+TG+TT N+E + Sbjct: 181 KPSSILMKYASDRLGLAPEETYVIGDNIRTDIAAGVHAGCKTVLVMTGITTDRNMEAHME 240 Query: 254 LTGVEPDEICADLAELMVTL 273 GV PD IC DL+E++ L Sbjct: 241 AAGVTPDYICRDLSEVISLL 260 >B1DLQ0.1 B1DLQ0_9BACL HAD-superfamily subfamily IIA hydrolase like protein Length = 270 Score = 259 bits (662), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 128/244 (52%), Positives = 174/244 (71%) Query: 27 TLYHGGRMIQGADLLVAALRTAGIPFMFVTNNSSRTPASVAAHLCAMGIEAEAQEVCTSS 86 TLYHG + I+GAD L+ LR +P+ FVTNNS+ +P +VA L MGI+AE +EVCTS+ Sbjct: 18 TLYHGTQRIEGADQLIRQLREWKLPYRFVTNNSTVSPEAVAERLRKMGIDAEPREVCTSA 77 Query: 87 LAAARYIAGESPGATVAILGEEGLIQACREAGLRLVSEAPEYVVQGIDRSFTYDSLAMAS 146 AAA+YIA + PGA+V ++GE GLI+A AGL+L E P++V+QG+DR +Y+ L A Sbjct: 78 QAAAQYIANQKPGASVLVIGESGLIEAVEAAGLQLTEEQPDFVLQGLDRQLSYEQLTRAV 137 Query: 147 RWVREGAKFVLTNPDLMLPSDDGVMPGAGSLGAAIEAASGIPPVVIGKPEAHLIAYATSL 206 R + +GA+FVLTNPDL+LP + G+ PGAGS+GA + AA G P +IGKP L+ Y+ Sbjct: 138 RSILQGAEFVLTNPDLLLPGEGGLFPGAGSIGAMLTAAGGKEPTLIGKPSKILMDYSLRQ 197 Query: 207 MGIDPGEAVVVGDNMRTDISAGARAGCRTILVLTGLTTRDNLEDYQRLTGVEPDEICADL 266 +G+ + V+GDN+ TDI+AG +GC T+LVLTGLTTRDNL+ Y G PD IC DL Sbjct: 198 IGLTAEDTWVIGDNLATDIAAGHASGCGTVLVLTGLTTRDNLDYYAERAGCRPDVICDDL 257 Query: 267 AELM 270 +L+ Sbjct: 258 HKLL 261 >C2PM83.1 C2PM83_BACCE Putative uncharacterized protein Length = 255 Score = 226 bits (576), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 115/252 (45%), Positives = 162/252 (64%), Gaps = 4/252 (1%) Query: 19 GLLIDLDGTLYHGGRMIQGADLLVAALRTAGIPFMFVTNNSSRTPASVAAHLCAMGIEAE 78 G LIDLDGT+Y G I+ A V AL GIP++FVTNNS+R P VA L I AE Sbjct: 5 GYLIDLDGTMYRGEEQIEEASDFVKALGERGIPYLFVTNNSTRKPEQVAEKLVRFDIPAE 64 Query: 79 AQEVCTSSLAAARYIAGESPGATVAILGEEGLIQACREAGLRLVSEAPEYVVQGIDRSFT 138 A++V T+S+A A +I ATV ++GEEGL A E G LV E P++VV G+DR T Sbjct: 65 AEQVFTTSMATANFIYERKQDATVYMIGEEGLHDALVEKGFELVDENPDFVVVGLDRDIT 124 Query: 139 YDSLAMASRWVREGAKFVLTNPDLMLPSDDGVMPGAGSLGAAIEAASGIPPVVIGKPEAH 198 Y+ LA A VR GA F+ TN D+ +P++ G++PG GSL + + ++G+ P+ IGKPE+ Sbjct: 125 YEKLAKACLAVRNGATFISTNGDIAIPTERGLLPGNGSLTSVVAVSTGVDPIFIGKPESI 184 Query: 199 LIAYATSLMGIDPGEAVVVGDNMRTDISAGARAGCRTILVLTGLTTRDNLEDYQRLTGVE 258 ++ A ++GI+ EA++VGDN TDI AG AG T+LV TG+TT + L +Y+ V+ Sbjct: 185 IMEQALKVLGIEKNEALMVGDNYDTDILAGINAGMHTLLVHTGVTTVEKLTEYE----VQ 240 Query: 259 PDEICADLAELM 270 P ++ +L E + Sbjct: 241 PTQVVHNLTEWI 252 >B5V7K6.1 B5V7K6_BACCE Phosphatase,haloacid dehalogenase family Length = 254 Score = 226 bits (575), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 115/252 (45%), Positives = 162/252 (64%), Gaps = 4/252 (1%) Query: 19 GLLIDLDGTLYHGGRMIQGADLLVAALRTAGIPFMFVTNNSSRTPASVAAHLCAMGIEAE 78 G LIDLDGT+Y G I+ A V AL GIP++FVTNNS+R P VA L I A+ Sbjct: 4 GYLIDLDGTMYRGEEQIEEASDFVKALGERGIPYLFVTNNSTRKPEQVAEKLVRFDIPAK 63 Query: 79 AQEVCTSSLAAARYIAGESPGATVAILGEEGLIQACREAGLRLVSEAPEYVVQGIDRSFT 138 A++V T+S+A A +I ATV ++GEEGL A E G LV E P++VV G+DR T Sbjct: 64 AEQVFTTSMATANFIYERKQDATVYMIGEEGLHDALVEKGFELVDENPDFVVVGLDRDIT 123 Query: 139 YDSLAMASRWVREGAKFVLTNPDLMLPSDDGVMPGAGSLGAAIEAASGIPPVVIGKPEAH 198 Y+ LA A VR GA F+ TN D+ +P++ G++PG GSL + + ++G+ P+ IGKPE+ Sbjct: 124 YEKLAKACLAVRNGATFISTNGDIAIPTERGLLPGNGSLTSVVAVSTGVDPIFIGKPESI 183 Query: 199 LIAYATSLMGIDPGEAVVVGDNMRTDISAGARAGCRTILVLTGLTTRDNLEDYQRLTGVE 258 ++ A L+GI+ EA++VGDN TDI AG AG T+LV TG+TT + L +Y+ V+ Sbjct: 184 IMEQALKLLGIEKNEALMVGDNYDTDILAGINAGMHTLLVHTGVTTVEKLTEYE----VQ 239 Query: 259 PDEICADLAELM 270 P ++ +L E + Sbjct: 240 PTQVVHNLTEWI 251 >Q81XP1.1 Q81XP1_BACAN Phosphatase,haloacid dehalogenase family Length = 254 Score = 225 bits (574), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 114/252 (45%), Positives = 162/252 (64%), Gaps = 4/252 (1%) Query: 19 GLLIDLDGTLYHGGRMIQGADLLVAALRTAGIPFMFVTNNSSRTPASVAAHLCAMGIEAE 78 G LIDLDGT+Y G I+ A V AL GIP++FVTNNS+R P VA L I A+ Sbjct: 4 GYLIDLDGTMYRGEEQIEEASDFVKALGERGIPYLFVTNNSTRKPEQVAEKLVRFDIPAK 63 Query: 79 AQEVCTSSLAAARYIAGESPGATVAILGEEGLIQACREAGLRLVSEAPEYVVQGIDRSFT 138 A++V T+S+A A +I ATV ++GEEGL A E G LV E P++VV G+DR T Sbjct: 64 AEQVFTTSMATANFIYERKQDATVYMIGEEGLHDALVEKGFELVDENPDFVVVGLDRDIT 123 Query: 139 YDSLAMASRWVREGAKFVLTNPDLMLPSDDGVMPGAGSLGAAIEAASGIPPVVIGKPEAH 198 Y+ LA A VR GA F+ TN D+ +P++ G++PG GSL + + ++G+ P+ IGKPE+ Sbjct: 124 YEKLAKACLAVRNGATFISTNGDIAIPTERGLLPGNGSLTSVVAVSTGVDPIFIGKPESI 183 Query: 199 LIAYATSLMGIDPGEAVVVGDNMRTDISAGARAGCRTILVLTGLTTRDNLEDYQRLTGVE 258 ++ A ++GI+ EA++VGDN TDI AG AG T+LV TG+TT + L +Y+ V+ Sbjct: 184 IMEQALKVLGIEKNEALIVGDNYDTDILAGINAGMHTLLVHTGVTTVEKLTEYE----VQ 239 Query: 259 PDEICADLAELM 270 P ++ +L E + Sbjct: 240 PTQVVHNLTEWI 251 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,595,068,254 Number of extensions: 64700802 Number of successful extensions: 217506 Number of sequences better than 1.0e-05: 1262 Number of HSP's gapped: 216031 Number of HSP's successfully gapped: 1265 Length of query: 275 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 141 Effective length of database: 1,798,280,193 Effective search space: 253557507213 Effective search space used: 253557507213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)