BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0026.p (261 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6R4.1 B3K6R4_9BACI HAD-superfamily hydrolase, subfamily IA, v... 335 2e-90 Q9KAY1.1 Q9KAY1_BACHD BH2155 protein 272 2e-71 A7Z7I0.1 A7Z7I0_BACA2 YsaA 271 4e-71 Q65GA6.1 Q65GA6_BACLD YsaA 266 1e-69 Q62RR1.2 Q62RR1_BACLD Putative metallopeptidase YsaA 266 2e-69 >B3K6R4.1 B3K6R4_9BACI HAD-superfamily hydrolase, subfamily IA, variant 1 Length = 263 Score = 335 bits (860), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 164/263 (62%), Positives = 192/263 (73%), Gaps = 2/263 (0%) Query: 1 MPISAVLFDLDDTLLWDERSVEEAFRYASEA--AGDSVDPXXXXXXXXXXXXNLYESYET 58 MPI+AVLFDLDDTLLWDERSVEEAF +A A VD LYESY+T Sbjct: 1 MPIAAVLFDLDDTLLWDERSVEEAFEVTCQAGAAETGVDAGALLAAVREEARALYESYDT 60 Query: 59 FPFTKLIGINPFEALWGNFTAGEQLEFRQMEQLAPVYRKESWRRGLAALGVEDXXXXXXX 118 F FTK+IGINPFE LW NFTAG Q EFR++++LAPVYRKESWRRGL LGV++ Sbjct: 61 FAFTKMIGINPFEGLWANFTAGSQPEFRKLQELAPVYRKESWRRGLHRLGVDNEALAETL 120 Query: 119 XXXXXXXRRSRPYIYEETLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVV 178 RR+RPY+YEET +VL EL+G VKLLLLTNG P LQQEKLDGVPELVPYFDH++ Sbjct: 121 AAQFAAERRTRPYVYEETFEVLKELQGNVKLLLLTNGSPDLQQEKLDGVPELVPYFDHII 180 Query: 179 ISGNFGKGKPDKGIFTHAMNLLDITPEEGIMVGDKLTTDILGGLGAGLTTVWINRTGKAS 238 ISG+FGKGKPD IF HA++LL + ++ +MVGDKLTTDI GG AGL TVWINR + Sbjct: 181 ISGDFGKGKPDVSIFKHALDLLGVEADQAVMVGDKLTTDIKGGNAAGLHTVWINRVDRPH 240 Query: 239 DPEITPDYQIGHLSELLPLVETL 261 DP I P ++I HLSEL +V +L Sbjct: 241 DPAIQPKFEIKHLSELHAIVASL 263 >Q9KAY1.1 Q9KAY1_BACHD BH2155 protein Length = 267 Score = 272 bits (695), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 134/261 (51%), Positives = 173/261 (66%), Gaps = 4/261 (1%) Query: 3 ISAVLFDLDDTLLWDERSVEEAFRYASEAAGD--SVDPXXXXXXXXXXXXNLYESYETFP 60 + A++FDLDDTLLWD++SV+EAF + A S+DP LY SYET+ Sbjct: 2 VKAIIFDLDDTLLWDKKSVKEAFVATCQYAATKRSLDPEALEESVREVARELYASYETYE 61 Query: 61 FTKLIGINPFEALWGNFTAGEQLEFRQMEQLAPVYRKESWRRGLAALGVEDXXXXXXXXX 120 FT++IGINPFE LWG F FR+M+++ P YRK++W +GLA LG+ED Sbjct: 62 FTQMIGINPFEGLWGQFLDDND-HFRKMKEIVPTYRKDAWTQGLAKLGIEDQALGEELAE 120 Query: 121 XXXXXRRSRPYIYEETLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVVIS 180 R+ P++YEET +VLD+LKG +LLLLTNG P LQQ KLD PELVPYFDH+VIS Sbjct: 121 RFPEERKKHPFVYEETFRVLDQLKGNYQLLLLTNGSPHLQQTKLDITPELVPYFDHIVIS 180 Query: 181 GNFGKGKPDKGIFTHAMNLLDITPEEGIMVGDKLTTDILGGLGAGLTTVWINRTGKASDP 240 G FG+GKPD IF HA++LL +T +E IMVGD L TDILG G+ +VWINR K Sbjct: 181 GGFGRGKPDPSIFEHALSLLSLTKDEAIMVGDNLMTDILGASRVGIKSVWINREQKEQG- 239 Query: 241 EITPDYQIGHLSELLPLVETL 261 E+ P Y+I HL +LL +++ L Sbjct: 240 EVEPTYEIEHLEQLLDILKKL 260 >A7Z7I0.1 A7Z7I0_BACA2 YsaA Length = 264 Score = 271 bits (693), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 132/259 (50%), Positives = 168/259 (64%), Gaps = 2/259 (0%) Query: 3 ISAVLFDLDDTLLWDERSVEEAFRYASEAAGDS--VDPXXXXXXXXXXXXNLYESYETFP 60 + AV FDLDDTLLWDE+SV FR A +DP LY SYET+P Sbjct: 1 MKAVFFDLDDTLLWDEKSVRTTFRETCLQAEKKYGLDPHTFEDAVRKAARELYMSYETYP 60 Query: 61 FTKLIGINPFEALWGNFTAGEQLEFRQMEQLAPVYRKESWRRGLAALGVEDXXXXXXXXX 120 +T +IGINPFE LW NF+ F+Q+ ++AP YR+ +W GL ++G++D Sbjct: 61 YTVMIGINPFEGLWANFSEPVSEGFKQLNRIAPEYRRNAWTNGLKSVGIDDAAYGEYLAE 120 Query: 121 XXXXXRRSRPYIYEETLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVVIS 180 RR RPY+Y+ET VL++LKG +LLLLTNG P+LQ+EKL GVPEL PYF+ +VIS Sbjct: 121 FFAAERRQRPYVYDETFAVLEKLKGVYELLLLTNGDPSLQKEKLAGVPELAPYFNEIVIS 180 Query: 181 GNFGKGKPDKGIFTHAMNLLDITPEEGIMVGDKLTTDILGGLGAGLTTVWINRTGKASDP 240 G FGKGKPD IF H + LL++ E+ IMVGD L TDILG AG+TTVW+NRT K +D Sbjct: 181 GAFGKGKPDASIFEHCLGLLNLKKEDAIMVGDNLNTDILGAKRAGITTVWVNRTQKKNDT 240 Query: 241 EITPDYQIGHLSELLPLVE 259 +I PDY I L EL ++E Sbjct: 241 DIAPDYVISDLHELFAIIE 259 >Q65GA6.1 Q65GA6_BACLD YsaA Length = 271 Score = 266 bits (680), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 128/258 (49%), Positives = 167/258 (64%), Gaps = 2/258 (0%) Query: 3 ISAVLFDLDDTLLWDERSVEEAFRYASEAAGDS--VDPXXXXXXXXXXXXNLYESYETFP 60 + AV FDLDDTLLWDE+SV AF A + + LY SYET+P Sbjct: 2 MKAVFFDLDDTLLWDEKSVSTAFSKTCLKAEEKYGIHAEEFEAAVREAARKLYMSYETYP 61 Query: 61 FTKLIGINPFEALWGNFTAGEQLEFRQMEQLAPVYRKESWRRGLAALGVEDXXXXXXXXX 120 +T +IGINPFE LW NF+ F+++ ++ P YR+ +W GL A G++D Sbjct: 62 YTVMIGINPFEGLWSNFSEPISEGFQKLNKIVPEYRRNAWTNGLKAFGIDDPAFGEELGE 121 Query: 121 XXXXXRRSRPYIYEETLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVVIS 180 RR P++YEET VLDELKGKV+LLLLTNG P+LQ+EKL GVPEL PYF+ +VIS Sbjct: 122 YFAAVRRKSPFVYEETFAVLDELKGKVELLLLTNGDPSLQKEKLAGVPELAPYFNEIVIS 181 Query: 181 GNFGKGKPDKGIFTHAMNLLDITPEEGIMVGDKLTTDILGGLGAGLTTVWINRTGKASDP 240 G FGKGKPD IF H + LL +T ++ +MVGD L TDILG AG+ TVW+NR GK ++ Sbjct: 182 GEFGKGKPDPSIFEHCLTLLGMTKDDAVMVGDNLNTDILGASRAGIQTVWVNRKGKKNET 241 Query: 241 EITPDYQIGHLSELLPLV 258 ++ PD++I HLSEL ++ Sbjct: 242 DVAPDHEISHLSELFDIL 259 >Q62RR1.2 Q62RR1_BACLD Putative metallopeptidase YsaA Length = 270 Score = 266 bits (679), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 128/258 (49%), Positives = 167/258 (64%), Gaps = 2/258 (0%) Query: 3 ISAVLFDLDDTLLWDERSVEEAFRYASEAAGDS--VDPXXXXXXXXXXXXNLYESYETFP 60 + AV FDLDDTLLWDE+SV AF A + + LY SYET+P Sbjct: 1 MKAVFFDLDDTLLWDEKSVSTAFSKTCLKAEEKYGIHAEEFEAAVREAARKLYMSYETYP 60 Query: 61 FTKLIGINPFEALWGNFTAGEQLEFRQMEQLAPVYRKESWRRGLAALGVEDXXXXXXXXX 120 +T +IGINPFE LW NF+ F+++ ++ P YR+ +W GL A G++D Sbjct: 61 YTVMIGINPFEGLWSNFSEPISEGFQKLNKIVPEYRRNAWTNGLKAFGIDDPAFGEELGE 120 Query: 121 XXXXXRRSRPYIYEETLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVVIS 180 RR P++YEET VLDELKGKV+LLLLTNG P+LQ+EKL GVPEL PYF+ +VIS Sbjct: 121 YFAAVRRKSPFVYEETFAVLDELKGKVELLLLTNGDPSLQKEKLAGVPELAPYFNEIVIS 180 Query: 181 GNFGKGKPDKGIFTHAMNLLDITPEEGIMVGDKLTTDILGGLGAGLTTVWINRTGKASDP 240 G FGKGKPD IF H + LL +T ++ +MVGD L TDILG AG+ TVW+NR GK ++ Sbjct: 181 GEFGKGKPDPSIFEHCLTLLGMTKDDAVMVGDNLNTDILGASRAGIQTVWVNRKGKKNET 240 Query: 241 EITPDYQIGHLSELLPLV 258 ++ PD++I HLSEL ++ Sbjct: 241 DVAPDHEISHLSELFDIL 258 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.139 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,507,496,104 Number of extensions: 59241563 Number of successful extensions: 131438 Number of sequences better than 1.0e-05: 1152 Number of HSP's gapped: 130850 Number of HSP's successfully gapped: 1152 Length of query: 261 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 128 Effective length of database: 1,807,701,208 Effective search space: 231385754624 Effective search space used: 231385754624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)