BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0012.p (429 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DMN4.1 B1DMN4_9BACL GTP-binding proten HflX 565 e-159 C0ZEX4.1 C0ZEX4_BREBN Probable GTP-binding protein HflX 382 e-104 A4J3Z0.1 A4J3Z0_DESRM GTP-binding protein, HSR1-related 346 2e-93 B1I3R4.1 B1I3R4_DESAP GTP-binding protein, HSR1-related 346 2e-93 A1HMU1.1 A1HMU1_9FIRM Small GTP-binding protein 344 9e-93 >B1DMN4.1 B1DMN4_9BACL GTP-binding proten HflX Length = 429 Score = 565 bits (1457), Expect = e-159, Method: Compositional matrix adjust. Identities = 291/425 (68%), Positives = 336/425 (79%), Gaps = 2/425 (0%) Query: 5 THDTETEIQDRAILVSLVTDKIKRTGVDPELSLQELVQLAETAGVIVLDVLRQNKETPDS 64 T +T + +RA+LVSLVT IKR DPE SL+ELV LAETAG VL + QNKE PD+ Sbjct: 5 TFNTTATMLERAVLVSLVTPNIKRGISDPEHSLEELVALAETAGTEVLTTMVQNKEAPDT 64 Query: 65 KWFIGKGKVEELRMAADGLGANTAIFDQELSGAQVRNLEEALDLKIIDRTQLILDIFAGR 124 KWFIGKGKVEELR AD LGA TAIFDQELSGAQVRNLEE LDLKI+DRTQLILDIFA R Sbjct: 65 KWFIGKGKVEELRAIADELGATTAIFDQELSGAQVRNLEETLDLKIVDRTQLILDIFAQR 124 Query: 125 AKTREGIIQVELAQLSYLLPRLSGHGKNLSRLXXXXXXXXXXESKLETDRRHIRDRISEL 184 AKTREGIIQVELAQLSYLLPRLSGHGKNLSRL ESKLETDRRHIR RI++L Sbjct: 125 AKTREGIIQVELAQLSYLLPRLSGHGKNLSRLGGGIGTRGPGESKLETDRRHIRGRITDL 184 Query: 185 KRQLDEVVKTRELHRERRRKSGAVQVALVGYTNAGKSTLLKQLTDADVYIENQLFATLDP 244 K QLDEVV+ R+LHRERR+KSG +QVALVGYTNAGKSTLL++LT ADVY+ENQLFATLDP Sbjct: 185 KAQLDEVVRHRKLHRERRKKSGVIQVALVGYTNAGKSTLLRELTSADVYVENQLFATLDP 244 Query: 245 TSRVLQLPAGKEVVLTDTVGFIQNLPHDLVASFRATLEEVNEANLVLHVVDASSPMREEQ 304 TSR L+LP G+EVVLTDTVGFIQNLPHDLVA+FRATLEEV EA+LVLHVVD SSPMREEQ Sbjct: 245 TSRTLELPNGREVVLTDTVGFIQNLPHDLVAAFRATLEEVLEADLVLHVVDGSSPMREEQ 304 Query: 305 MEVVQSILQDLGAAGKPQIVLFNKSDICRPEQLQMLPSGPGFLKISAFNPDDLTRITEMI 364 M VV+ IL DLGAAG + ++NK D+C +++ SG L ISA+ DDL + I Sbjct: 305 MRVVEQILGDLGAAGTASLTIYNKKDLCEVNHVELPTSGTDTLVISAYQKDDLELVRNKI 364 Query: 365 ADELAGDTLTFRIPGDRGDLSSLLYRVGEVLEQNV-EDNDVL-YQVRLNKEDYGKWSYKL 422 D+L GDT+TF +P DRGDL +L YR GEV+ +V ED + L V+++++ Y + YKL Sbjct: 365 QDKLTGDTVTFTLPADRGDLIALAYRSGEVVGNDVSEDGESLRLTVQVSRQAYEQNGYKL 424 Query: 423 AEYVE 427 Y+E Sbjct: 425 QPYLE 429 >C0ZEX4.1 C0ZEX4_BREBN Probable GTP-binding protein HflX Length = 430 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 214/413 (51%), Positives = 287/413 (69%), Gaps = 14/413 (3%) Query: 13 QDRAILVSLVTDKIKRTGVDPELSLQELVQLAETAGVIVLDVLRQNKETPDSKWFIGKGK 72 Q+ AILV D R LS++EL +LA+TAGV VLDV+ QN++ DS W++G GK Sbjct: 8 QETAILVGCYLDN--RDEERTRLSMEELHELADTAGVEVLDVITQNRDRVDSAWYLGTGK 65 Query: 73 VEELRMAADGLGANTAIFDQELSGAQVRNLEEALDLKIIDRTQLILDIFAGRAKTREGII 132 ++E+ A+ L + IF+ ELS +Q RNL++ D K+IDRTQLILDIFAGRA++REG I Sbjct: 66 IDEIAQRAEELDVDVIIFNDELSPSQTRNLDKVFDCKVIDRTQLILDIFAGRAQSREGKI 125 Query: 133 QVELAQLSYLLPRLSGHGKNLSRLXXXXXXXXXXESKLETDRRHIRDRISELKRQLDEVV 192 QVELAQ +YLLPRL+G GK LSRL E+KLE+DRRHIR RISELK+QL++ V Sbjct: 126 QVELAQYNYLLPRLAGQGKQLSRLGGGIGTRGPGETKLESDRRHIRKRISELKQQLEDTV 185 Query: 193 KTRELHRERRRKSGAVQVALVGYTNAGKSTLLKQLTDADVYIENQLFATLDPTSRVLQLP 252 +TR+LHRERR+K+ Q+ALVGYTNAGKST+L +LT+A+ E++LFATLDPT+R L+LP Sbjct: 186 RTRQLHRERRKKNNVFQIALVGYTNAGKSTILNKLTNANTLQEDKLFATLDPTTRQLELP 245 Query: 253 AGKEVVLTDTVGFIQNLPHDLVASFRATLEEVNEANLVLHVVDASSPMREEQMEVVQSIL 312 +G +V+LTDTVGFIQ+LP LVA+FR+TLE V EA+L+LHVVD+ P + MEVV IL Sbjct: 246 SGLDVLLTDTVGFIQDLPTSLVAAFRSTLEGVKEADLILHVVDSHHPDFQVHMEVVDKIL 305 Query: 313 QDLGAAGKPQIVLFNKSDICRPEQLQMLPSGPGFLKISAFNPDD----LTRITEMIADEL 368 ++L A P +V+FNK+D+ + LP + ISA DD LTRI Sbjct: 306 RELKAEEIPHLVVFNKADML--TEGSYLPRADESILISAMREDDLKQLLTRIESFALQSF 363 Query: 369 AGDTLTFRIPGDRGDLSSLLYRVGEVLEQNVEDNDVLY--QVRLNKED--YGK 417 + + R+P +RGD+ SLL+R G +EQ + + Y VR+NK++ YG+ Sbjct: 364 --NAMKLRVPVERGDILSLLHRDGVEMEQEFNEEEAAYLITVRVNKDNPIYGR 414 >A4J3Z0.1 A4J3Z0_DESRM GTP-binding protein, HSR1-related Length = 421 Score = 346 bits (888), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 196/394 (49%), Positives = 260/394 (65%), Gaps = 5/394 (1%) Query: 6 HDTETEIQDRAILVSLVTDKIKRTGVDPELSLQELVQLAETAGVIVLDVLRQNKETPDSK 65 HD +T I++RA+LV + + SL EL LA TAG V+ Q+++ PDS Sbjct: 2 HDIKTTIEERAVLVGVKLPGFHEHQICQ--SLDELANLASTAGADVMGTFMQSRQYPDSA 59 Query: 66 WFIGKGKVEELRMAADGLGANTAIFDQELSGAQVRNLEEALDLKIIDRTQLILDIFAGRA 125 FIGKGK +EL + AN + D+ELS AQ RNLE+ L +K+IDRTQLILDIFA RA Sbjct: 60 TFIGKGKAQELAEYCREIHANMVVIDRELSPAQARNLEDRLGVKVIDRTQLILDIFARRA 119 Query: 126 KTREGIIQVELAQLSYLLPRLSGHGKNLSRLXXXXXXXXXXESKLETDRRHIRDRISELK 185 +TREG +QVELAQL YLLPRL+G G LSRL E+KLE DRR IR RIS+L+ Sbjct: 120 QTREGKLQVELAQLKYLLPRLTGLGTQLSRLGGGIGTRGPGETKLEVDRRRIRKRISDLE 179 Query: 186 RQLDEVVKTRELHRERRRKSGAVQVALVGYTNAGKSTLLKQLTDADVYIENQLFATLDPT 245 ++L EV + R L R+ R+ V+LVGYTNAGKSTLLK LT AD+ +E++LFATLDPT Sbjct: 180 QELKEVQRHRSLLRKSRKSEPLPIVSLVGYTNAGKSTLLKSLTGADILVEDKLFATLDPT 239 Query: 246 SRVLQLPAGKEVVLTDTVGFIQNLPHDLVASFRATLEEVNEANLVLHVVDASSPMREEQM 305 +R + LP V+LTDTVGFIQNLPH LVA+FRATLEEV E++L+LH+VDAS P E Q+ Sbjct: 240 TRRISLPNNDNVLLTDTVGFIQNLPHHLVAAFRATLEEVQESDLLLHIVDASHPNYEGQI 299 Query: 306 EVVQSILQDLGAAGKPQIVLFNKSDICRPEQLQMLPSGPGFLKISAFNPDDLTRITEMIA 365 V+++L+ L KP I++FNK D+ + Q P + ISA + + ++ +MIA Sbjct: 300 RAVETVLESLHVLDKPSIMVFNKIDMVKDVQEIPFTENPR-VYISATSGEGSDQLLDMIA 358 Query: 366 DELA--GDTLTFRIPGDRGDLSSLLYRVGEVLEQ 397 L L +P D+ +L S+L++ G+VL + Sbjct: 359 GVLKERYSILKLTVPYDKTNLISILHQKGKVLNE 392 >B1I3R4.1 B1I3R4_DESAP GTP-binding protein, HSR1-related Length = 422 Score = 346 bits (888), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 197/418 (47%), Positives = 270/418 (64%), Gaps = 10/418 (2%) Query: 14 DRAILVSLVTDKIKRTGVDPELSLQELVQLAETAGVIVLDVLRQNKETPDSKWFIGKGKV 73 ++A+LV + + TG + SL EL +LA TAG V + Q + PD F+G+GK Sbjct: 10 EKAVLVGVELGSL--TGAEVRESLNELNRLARTAGAEVAGTVLQKRPRPDPAHFVGRGKA 67 Query: 74 EELRMAADGLGANTAIFDQELSGAQVRNLEEALDLKIIDRTQLILDIFAGRAKTREGIIQ 133 EEL GA IFDQ+LS AQ RNLE ++++DRTQ+ILDIFA RA+TREG +Q Sbjct: 68 EELAALCSAAGAGLLIFDQDLSPAQARNLENVTGVRVLDRTQIILDIFARRARTREGKLQ 127 Query: 134 VELAQLSYLLPRLSGHGKNLSRLXXXXXXXXXXESKLETDRRHIRDRISELKRQLDEVVK 193 VELAQL+Y+LPRL+G G LSRL E+KLETDRR IR RI++L+R++ EV + Sbjct: 128 VELAQLNYVLPRLTGRGTELSRLGGGIGTRGPGETKLETDRRRIRQRIADLQREIAEVRR 187 Query: 194 TRELHRERRRKSGAVQVALVGYTNAGKSTLLKQLTDADVYIENQLFATLDPTSRVLQLPA 253 R+L R R+ + VALVGYTNAGKS+LL LT A V +E++LFATLDPTSR L+LP Sbjct: 188 HRQLLRRARKVAPVPLVALVGYTNAGKSSLLNALTGAVVSVEDRLFATLDPTSRQLRLPT 247 Query: 254 GKEVVLTDTVGFIQNLPHDLVASFRATLEEVNEANLVLHVVDASSPMREEQMEVVQSILQ 313 + VVLTDTVGFI++LPH LVA+FRATLEEV EA+L+LHVVD S P + + V +L+ Sbjct: 248 NEVVVLTDTVGFIRHLPHHLVAAFRATLEEVVEADLLLHVVDLSHPAHQAHITAVDGVLE 307 Query: 314 DLGAAGKPQIVLFNKSDICRPEQLQMLPSGPGFLKISAFNPDDLTRITEMIADELAG--D 371 +LGA GKP++++FNK+D+ P +L +L G + +SA L + +AD L+ Sbjct: 308 ELGAGGKPRLMVFNKTDLVEPGELDLL--GRNDVAVSALTGAGLDTLRAAVADALSTWRT 365 Query: 372 TLTFRIPGDRGDLSSLLYRVGEVLEQNVEDNDVLYQVRLNKEDYGKWSYKLAEYVEQE 429 F IP R L +L Y G VL ++ ++ V +V L W+ ++A +E++ Sbjct: 366 RERFLIPYSRTQLVALAYEHGRVLAEHHREDGVEIEVELPV----AWARRIAARLEEQ 419 >A1HMU1.1 A1HMU1_9FIRM Small GTP-binding protein Length = 597 Score = 344 bits (883), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 199/419 (47%), Positives = 273/419 (65%), Gaps = 11/419 (2%) Query: 14 DRAILVSLVTDKIKRTGVDPELSLQELVQLAETAGVIVLDVLRQNKETPDSKWFIGKGKV 73 +RA+LV + D TG + SL+EL QLAETAG V+D++ Q +E PD+ +FIG+GKV Sbjct: 187 ERALLVGIEKD----TGWNISDSLKELAQLAETAGAQVVDIISQRRERPDAAFFIGRGKV 242 Query: 74 EELRMAADGLGANTAIFDQELSGAQVRNLEEALDLKIIDRTQLILDIFAGRAKTREGIIQ 133 E+ + N IFD ELS AQ RNLE+AL +K++DRT LILDIFA RA+TREG +Q Sbjct: 243 HEINLLRQSKDINLIIFDDELSPAQQRNLEQALGVKVLDRTALILDIFAQRARTREGKLQ 302 Query: 134 VELAQLSYLLPRLSGHGKNLSRLXXXXXXXXXXESKLETDRRHIRDRISELKRQLDEVVK 193 VELAQL Y LPRL G G LSRL E+KLE D+R IR+RI++++ +++++ K Sbjct: 303 VELAQLRYNLPRLGGQGLVLSRLGGGIGTRGPGETKLEVDKRRIRERIADIEAEIEQIKK 362 Query: 194 TRELHRERRRKSGAVQVALVGYTNAGKSTLLKQLTDADVYIENQLFATLDPTSRVLQLPA 253 R LHRERR+++ +ALVGYTNAGKSTLL LT+A V E++LFATLDPT+R L+LP Sbjct: 363 HRSLHRERRQRAHVPTLALVGYTNAGKSTLLNTLTNASVLAEDKLFATLDPTTRRLKLPN 422 Query: 254 GKEVVLTDTVGFIQNLPHDLVASFRATLEEVNEANLVLHVVDASSPMREEQMEVVQSILQ 313 G+E +LTDTVGFIQ LPH L+A+FRATLEEV A+L+LH+VDAS P EEQM+ V +L+ Sbjct: 423 GQEALLTDTVGFIQKLPHQLIAAFRATLEEVVYADLLLHIVDASHPRYEEQMDSVFEVLR 482 Query: 314 DLGAAGKPQIVLFNKSD-ICRPEQLQMLPSGPGFLKISAFNPDDLTRITEMIADELAGDT 372 +L A K I +FNK D I P + L G + ISA + + E + ++L T Sbjct: 483 ELQADTKDIITVFNKIDKIENPNIIARLVRESGSVAISALTGLGIDSLLEQVQNKLRIIT 542 Query: 373 --LTFRIPGDRGDLSSLLYRVGEVLEQNVEDNDVLYQVRLNKEDYGKWSYKLAEYVEQE 429 + IP L S LY +G V+ + + +++ +L + + + Y++QE Sbjct: 543 TDVCLVIPYSETGLVSQLYDLGSVISIDYKTENIVVHAQLPLQ----YRKRFEPYMKQE 597 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,276,852,786 Number of extensions: 90861498 Number of successful extensions: 405360 Number of sequences better than 1.0e-05: 2195 Number of HSP's gapped: 405831 Number of HSP's successfully gapped: 2197 Length of query: 429 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 290 Effective length of database: 1,751,175,118 Effective search space: 507840784220 Effective search space used: 507840784220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)