BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0005.p (581 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6T0.1 B3K6T0_9BACI Penicillin-binding protein, 1A family 780 0.0 B1DG24.1 B1DG24_9BACL Penicillin-binding protein, 1A family 575 e-162 C0ZI00.1 C0ZI00_BREBN Penicillin binding protein 443 e-122 P38050.2 PBPF_BACSU Penicillin-binding protein 1F 411 e-112 A7Z322.1 A7Z322_BACA2 PbpF 407 e-111 >B3K6T0.1 B3K6T0_9BACI Penicillin-binding protein, 1A family Length = 893 Score = 780 bits (2014), Expect = 0.0, Method: Compositional matrix adjust. Identities = 367/535 (68%), Positives = 447/535 (83%), Gaps = 2/535 (0%) Query: 44 RVLWTLFFTTALAIFCALGGYLFIMLNGEKLLDENIDKLTVNETTKIYDRNAKLIGELAL 103 R+ W LFFT +AIFCAL GYLFI+++GEKLL++N DKLT T+K+YDRN L+GEL+L Sbjct: 37 RIAWALFFTAVIAIFCALAGYLFILISGEKLLEQNQDKLTAYGTSKVYDRNGTLMGELSL 96 Query: 104 EKSEPVEYEDIPKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEGGSTITQ 163 +KS+PV+YEDIP+ L+ AF+ATEDKRF EH+GVD+WSIGRAAVKDI ARSMVEGGSTITQ Sbjct: 97 QKSDPVKYEDIPEKLIQAFIATEDKRFMEHNGVDMWSIGRAAVKDIMARSMVEGGSTITQ 156 Query: 164 QLAKNIFLTRDKTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIKAASIRY 223 QLAKNIFLTRDKTFFRKATE+SIA+ALE + +K+EII YLNRINFGG YGIKAAS RY Sbjct: 157 QLAKNIFLTRDKTFFRKATEMSIALALERQHSKQEIIEMYLNRINFGGPYYGIKAASERY 216 Query: 224 FGQSNLNDLKVWQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYITKDQMDE 283 FG+S+L +L +W++ATLAAMPKGPSRYNPL+NP+LSKERR VVL LM +QGYIT ++ E Sbjct: 217 FGKSDLKELDLWEMATLAAMPKGPSRYNPLKNPDLSKERRAVVLTLMEQQGYITAEEAAE 276 Query: 284 AKVVNYNYKPPESKQRYQAFIDYAVDEAEDKFGLTEDDLNIGGYKIYTTMDAHAQETVEK 343 AK V+Y Y PPE +Q+Y AFIDY ++EAE+K+GLTEDD+NIGGY+IYTTMD +AQ +E+ Sbjct: 277 AKKVDYAYTPPERQQKYTAFIDYVMEEAEEKWGLTEDDVNIGGYQIYTTMDVNAQTAMEE 336 Query: 344 AFADSDNFEKSADDELVQGSMTIINQENGSIVALLGGRNYEKKGYSRVNGSRRSPGSSFK 403 F + NFE+S DD V+GSM IINQE G IVA++GGR Y + G+SR SRR PGS+ K Sbjct: 337 EFNNPANFEESPDDVQVEGSMVIINQETGGIVAMVGGREYTRGGFSRATDSRRQPGSALK 396 Query: 404 PLVAYAPALESGKFTNNSVLSNEKQCFGT-YCPNNLHGYSKTISMAAALTKSENIPAVWL 462 P+V+YAPALESGKFT +S LSN+KQCFG+ YCPNNLHGYS+TI M A+ +SENIPAVWL Sbjct: 397 PIVSYAPALESGKFTKDSRLSNKKQCFGSNYCPNNLHGYSETIGMPEAIQRSENIPAVWL 456 Query: 463 LNEIGVNTGFQFAKKLGIKLKDEDKNLSLALGGMSEGTNTLEMAQAYSAFANGGELREAY 522 LNEIGV TGF FA+ LGIK+ + D NLSLALGGM GTNT EMAQA+SAFANGGE +EA+ Sbjct: 457 LNEIGVKTGFDFAQSLGIKMSEGDANLSLALGGMDTGTNTFEMAQAFSAFANGGEFKEAF 516 Query: 523 SIKSITNSDGDTVYK-ANTKPERVMSENTAYQMTEMMQKVVEDGTGKKAKIDRPV 576 +IK I ++ G V++ +K +RVM E TA QMT+MM++VVEDGTGK A+I RPV Sbjct: 517 AIKQIKDNKGKVVHENKGSKGKRVMGETTASQMTDMMRRVVEDGTGKNARISRPV 571 >B1DG24.1 B1DG24_9BACL Penicillin-binding protein, 1A family Length = 860 Score = 575 bits (1483), Expect = e-162, Method: Compositional matrix adjust. Identities = 293/548 (53%), Positives = 395/548 (72%), Gaps = 16/548 (2%) Query: 40 LTKKRVLWTLFFTTALAIFCALGGYLFIMLNGEKLLDENIDKLTVNETTKIYDRNAKLIG 99 +T ++ + FFT AI C + GYLFI LNGE++L EN DKL + E + IYD + K + Sbjct: 24 MTPRKWFFAFFFTIVFAIVCGIIGYLFITLNGERILTENADKLELGEASIIYDADGKEVT 83 Query: 100 EL--ALEKSEPVEYEDIPKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEG 157 +L A E EY+++PKLL++AFVATED+RF+EHSG+D +SIGRA VKD+ ARS VEG Sbjct: 84 KLYDASANREIAEYDEMPKLLLDAFVATEDQRFYEHSGIDFFSIGRAVVKDVIARSKVEG 143 Query: 158 GSTITQQLAKNIFLTRDKTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIK 217 STITQQLAKNIFLT DKTFFRKATE SIAVALENK++K+EI+T YLNRI FG IYGIK Sbjct: 144 ASTITQQLAKNIFLTADKTFFRKATEASIAVALENKYSKQEILTMYLNRITFGKGIYGIK 203 Query: 218 AASIRYFGQSNLNDLKVWQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYIT 277 A+ YF ++L DLK+WQIATLA MPK P+RYNP+ +PE S RR VVL+LMY+Q IT Sbjct: 204 GAADYYF-DTDLKDLKLWQIATLAGMPKAPNRYNPINDPESSMTRRAVVLKLMYDQKLIT 262 Query: 278 KDQMDEAKVVNYNYKPP-----ESKQRYQAFIDYAVDEAEDKF-GLTEDDLNIGGYKIYT 331 + +M+EAK V Y+PP + Y+A++DY ++EA G+TED+L IGGY+IYT Sbjct: 263 QQEMEEAKAV--VYEPPANIDQKKSDPYKAYVDYVIEEAVKVMPGMTEDELRIGGYRIYT 320 Query: 332 TMDAHAQETVEKAFADSDNFEKSADDELVQGSMTIINQENGSIVALLGGRNYEKKGYSRV 391 +++ AQ+ VEK FAD NFEKS DD+ QG+M I++ +G+I A+ GGR+Y +KG +RV Sbjct: 321 SLNTTAQQAVEKEFADDSNFEKSPDDQKAQGAMIIMDHRDGTIQAMSGGRDYVRKGLNRV 380 Query: 392 NGSRRSPGSSFKPLVAYAPALESGKFTNNSVLSNEKQ-CFGTYCPNNLHGYSKTISMAAA 450 +R PGSSFKP+V+Y PALESG + +VL+ + Q CFG YCP + HG + ++M A Sbjct: 381 E-VKRQPGSSFKPIVSYGPALESGDYYPWTVLNQDSQKCFGDYCPRDPHG-AAPVTMQQA 438 Query: 451 LTKSENIPAVWLLNEIGVNTGFQFAKKLGIKLKDEDKNLSLALGGMSEGTNTLEMAQAYS 510 + +S N+ AVW LN++G+ G FAKKLGI+L ED+NL++ALGG+++G ++MA+AYS Sbjct: 439 IKESRNLAAVWTLNQVGIGNGIAFAKKLGIELAPEDRNLAIALGGLTQGVTPMQMAEAYS 498 Query: 511 AFANGGELREAYSIKSITNSDGDTVYKANTK-PERVMSENTAYQMTEMMQKVVED-GTGK 568 FANGG+ + ++I I + VY + +++M TA+ MT+MMQ V+E GTG Sbjct: 499 VFANGGKSVDPHTITKIETAANKKVYTYHAPAAKQLMKPETAWYMTQMMQTVLESGGTGT 558 Query: 569 KAKIDRPV 576 A+IDRPV Sbjct: 559 GARIDRPV 566 >C0ZI00.1 C0ZI00_BREBN Penicillin binding protein Length = 838 Score = 443 bits (1139), Expect = e-122, Method: Compositional matrix adjust. Identities = 233/540 (43%), Positives = 348/540 (64%), Gaps = 19/540 (3%) Query: 49 LFFTTALAIFCAL---GGYLFIMLNGEKLLDENIDKLT--VNETTKIYDRNAKLIGELAL 103 L + AIF L GGY ++ G++++++N+ KL E T IYD+N K I L Sbjct: 17 LMIAVSFAIFLVLSVIGGYFALLYAGDRMIEQNVQKLQDLKAEPTVIYDKNGKEIISLIR 76 Query: 104 EKS---EPVEYEDIPKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEGGST 160 +K+ +P+ ++PK L++AF+A EDKRF+EH GVD+ IG A V DI S+ EGGST Sbjct: 77 QKNRVYKPIN--EMPKTLIDAFLAVEDKRFYEHKGVDMVRIGGAIVNDIKKGSLAEGGST 134 Query: 161 ITQQLAKNIFLTRDKTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIKAAS 220 ITQQLA+N+FLT D+TF+RK E+SIA+ LE K++K++I+ YLNR+ G +G++ A+ Sbjct: 135 ITQQLARNVFLTLDQTFWRKTKEMSIAIGLERKYSKDQILEMYLNRVYLGEGEFGVEDAA 194 Query: 221 IRYFGQSNLNDLKVWQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYITKDQ 280 YFG+S ++++ V + A LAA+PK PS Y+P NPE +K RR V++LM EQG IT +Q Sbjct: 195 QYYFGKS-VSNVTVAEAAMLAAIPKAPSTYSPFNNPEKAKLRRDTVIRLMSEQGIITNEQ 253 Query: 281 MDEAKVVNYNYKPPES-----KQRYQAFIDYAVDEAEDKFGLTEDDLNIGGYKIYTTMDA 335 D A+ KP E K+ Y+AF D + E+E +FG+TE++L GG+ IYTT D Sbjct: 254 KDAAQAEALPTKPHEVAGGSLKKGYRAFFDNMLKESEAEFGVTEEELYRGGWDIYTTFDP 313 Query: 336 HAQETVEKAFADSDNFEKSADDELVQGSMTIINQENGSIVALLGGRNYEKKGYSRVNGSR 395 Q+ + + +A++ NF K V+ SM +I+ + G I ++GGRNY KG++ + Sbjct: 314 KVQDAMVEEYANAKNFPKDGAKRGVESSMIVIDAKTGGIAGMIGGRNYAPKGFNYATDMK 373 Query: 396 RSPGSSFKPLVAYAPAL--ESGKFTNNSVLSNEKQCFGTYCPNNLHG-YSKTISMAAALT 452 R PGSSFKP+ +APA+ + K+ NS LSN++Q F Y P N + Y++T+SM+ A+ Sbjct: 374 RQPGSSFKPIAVFAPAVNKDINKWNANSQLSNKRQSFNGYEPRNYNNRYTETVSMSRAMI 433 Query: 453 KSENIPAVWLLNEIGVNTGFQFAKKLGIKLKDEDKNLSLALGGMSEGTNTLEMAQAYSAF 512 S NIP VWLLNEIGV+ + KK+GI+L D+NL++ALGG+++G + +MAQAY+ F Sbjct: 434 DSANIPPVWLLNEIGVSESLAYLKKVGIELTPNDRNLAIALGGLNQGISPKQMAQAYTPF 493 Query: 513 ANGGELREAYSIKSITNSDGDTVYKANTKPERVMSENTAYQMTEMMQKVVEDGTGKKAKI 572 N G + EA+SI + N D V + K + A+++ M++K V++GTGK A+I Sbjct: 494 VNAGVVSEAHSISKVENKDEGVVREYQPKQSEALKPEAAWEIHTMLEKAVQEGTGKAARI 553 >P38050.2 PBPF_BACSU Penicillin-binding protein 1F Length = 714 Score = 411 bits (1056), Expect = e-112, Method: Compositional matrix adjust. Identities = 219/515 (42%), Positives = 320/515 (62%), Gaps = 11/515 (2%) Query: 55 LAIFCALGGYLFIMLNGEKLLDENIDKLTVNETTKIYDRNAKLIGELALEKSEPVEYEDI 114 L F AL GY+ I+ G ++DE KL ++ ++KI D+N + L E EPV +I Sbjct: 18 LTAFLALIGYISIIFLGHYVIDEK--KLILHASSKIVDQNGDEVASLYTENREPVSINEI 75 Query: 115 PKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEGGSTITQQLAKNIFLTRD 174 PK + AF+A EDKRF+EH G+D S+GRA +DI A VEGGSTITQQLAKNIFLT D Sbjct: 76 PKQVREAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGSTITQQLAKNIFLTHD 135 Query: 175 KTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIKAASIRYFGQSNLNDLKV 234 KTF RK EV IA+ LE ++K++++ YLN++ FG +YGI+AAS YF + + DL V Sbjct: 136 KTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAASHYYFNK-EVKDLTV 194 Query: 235 WQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYITKDQ--MDEAKVVNYNYK 292 + A LAA+PK PS Y+P+ +P+ +KERR +L +M +QGYI+ + + + + + K Sbjct: 195 SEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYISAKEAVTAQGRTLGLHVK 254 Query: 293 PPESKQRYQAFIDYAVDEAEDKFGLTEDDLNIGGYKIYTTMDAHAQETVEKAFADSDNFE 352 + ++ID + EAEDK+ ++ + L GGY I +D+ Q+T + + + Sbjct: 255 KQSETPWFDSYIDLVIKEAEDKYSISGEQLLQGGYTIKVPLDSKLQKTAYQVMKEGSYYP 314 Query: 353 KSADDELVQGSMTIINQENGSIVALLGGRNYEKKGYSRVNGSRRSPGSSFKPLVAYAPAL 412 + D+ +GS IN + G + A +GGR+Y KGY+RV R+ PGS+FKPL Y PA+ Sbjct: 315 GT--DQNAEGSAVFINNKTGGVEAAIGGRDYTSKGYNRVTAVRQ-PGSTFKPLAVYGPAM 371 Query: 413 ESGKFTNNSVLSNEKQCFGTYCPNNLHG-YSKTISMAAALTKSENIPAVWLLNEIGVNTG 471 + KF S+L +E Q +G Y P N Y ++M+ A+T S+N PAVW LNEIGV TG Sbjct: 372 QEKKFKPYSLLKDELQSYGDYTPKNYDSRYEGEVTMSDAITYSKNAPAVWTLNEIGVETG 431 Query: 472 FQFAKKLGIKLKDEDKNLSLALGGMSEGTNTLEMAQAYSAFANGGELREAYSIKSITNSD 531 + K GI + DE L+LALGG+ +G + L++A A+ FA G E + I SI + D Sbjct: 432 KSYLKANGIDIPDE--GLALALGGLEKGVSPLQLAGAFHTFAANGTYTEPFFISSIIDED 489 Query: 532 GDTVYKANTKPERVMSENTAYQMTEMMQKVVEDGT 566 G+T+ + +RV S+ T++ MT M+Q+VV+ GT Sbjct: 490 GETIADHKEEGKRVFSKQTSWNMTRMLQQVVKKGT 524 >A7Z322.1 A7Z322_BACA2 PbpF Length = 713 Score = 407 bits (1046), Expect = e-111, Method: Compositional matrix adjust. Identities = 218/535 (40%), Positives = 329/535 (61%), Gaps = 11/535 (2%) Query: 39 FLTKKRVLWTLFFTTALAIFCALGGYLFIMLNGEKLLDENIDKLTVNETTKIYDRNAKLI 98 L KK+ + A F AL GY+ I+ G ++DE KL ++ ++KI D+N I Sbjct: 1 MLKKKKRFFIPAVIAAATAFLALIGYIIIIFLGHYVIDEK--KLILHASSKIVDQNGDEI 58 Query: 99 GELALEKSEPVEYEDIPKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEGG 158 L E EPV + +P+ + +AF+A EDKRF+EH G+D+ S+GRA +D+ A VEGG Sbjct: 59 ASLYTENREPVSIQQVPEDVRDAFIAVEDKRFYEHHGIDVKSVGRAVFRDVLAGGKVEGG 118 Query: 159 STITQQLAKNIFLTRDKTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIKA 218 STITQQLAKNIFLT DKTF RK EV IA+ LE ++K++++ YLN++ FG +YGI+A Sbjct: 119 STITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQA 178 Query: 219 ASIRYFGQSNLNDLKVWQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYITK 278 AS YF + + DL V + A LAA+PK PS Y+P+++P+ +KERR +L +M +QGYI+ Sbjct: 179 ASHYYFNK-EVKDLTVAEGAVLAAIPKAPSLYSPIQHPDKNKERRDTILAMMNDQGYISA 237 Query: 279 DQMDEA--KVVNYNYKPPESKQRYQAFIDYAVDEAEDKFGLTEDDLNIGGYKIYTTMDAH 336 + A + + N K + ++ID + EAED++ ++ + L GGY I T++D+ Sbjct: 238 KEAVRAQGRTLGLNVKKTSETPWFDSYIDLVIKEAEDRYSMSGEQLLQGGYTIRTSLDSK 297 Query: 337 AQETVEKAFADSDNFEKSADDELVQGSMTIINQENGSIVALLGGRNYEKKGYSRVNGSRR 396 Q+T +S F + D+ +GS I+ + G + A +GGR+Y KGY+RV ++R Sbjct: 298 LQKTAYTLMKESGYFPGT--DQAAEGSAVFIDNKTGGVRAAVGGRDYVTKGYNRVT-AKR 354 Query: 397 SPGSSFKPLVAYAPALESGKFTNNSVLSNEKQCFGTYCPNNLHG-YSKTISMAAALTKSE 455 PGS+FKP+ Y PA++ KF S+L ++ + Y P N G Y ++M A+T S Sbjct: 355 QPGSTFKPIAVYGPAMQEDKFKPYSLLQDKLTSYDGYEPKNYDGQYKGEVTMQDAITYSI 414 Query: 456 NIPAVWLLNEIGVNTGFQFAKKLGIKLKDEDKNLSLALGGMSEGTNTLEMAQAYSAFANG 515 N PAVW L +IGV+TG + +K G+ + D L++ALGG+SEG L++A AY FA Sbjct: 415 NAPAVWTLKQIGVDTGKSYLEKNGMNI--SDNGLAIALGGLSEGVTPLQLAGAYHTFAAN 472 Query: 516 GELREAYSIKSITNSDGDTVYKANTKPERVMSENTAYQMTEMMQKVVEDGTGKKA 570 G + + I++IT+ DG+T+ RV S TA+ MT M+Q+VV++GT + Sbjct: 473 GMYTKPFFIENITDEDGETLSGPKNGKTRVFSGQTAWNMTRMLQQVVKEGTASQG 527 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,195,740,284 Number of extensions: 134685558 Number of successful extensions: 299376 Number of sequences better than 1.0e-05: 5081 Number of HSP's gapped: 280311 Number of HSP's successfully gapped: 6221 Length of query: 581 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 440 Effective length of database: 1,732,333,088 Effective search space: 762226558720 Effective search space used: 762226558720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)