BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0002.p (259 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6T2.1 B3K6T2_9BACI tRNA delta(2)-isopentenylpyrophosphate tra... 338 3e-91 B1DG22.1 B1DG22_9BACL tRNA delta(2)-isopentenylpyrophosphate tra... 323 1e-86 C0ZEY7.1 C0ZEY7_BREBN tRNA delta(2)-isopentenylpyrophosphate tra... 282 2e-74 Q2B1N1.1 Q2B1N1_9BACI tRNA delta(2)-isopentenylpyrophosphate tra... 277 6e-73 C2VFC7.1 C2VFC7_BACCE tRNA delta(2)-isopentenylpyrophosphate tra... 269 2e-70 >B3K6T2.1 B3K6T2_9BACI tRNA delta(2)-isopentenylpyrophosphate transferase EC=2.5.1.8 Length = 315 Score = 338 bits (866), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 158/240 (65%), Positives = 196/240 (81%) Query: 1 LTTETRRRVLVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMM 60 +T ET+ ++LVL+GPTAVGKT++S+E+A+ + EIISGDSMQVYRGMDIGTAKI EEM Sbjct: 1 MTDETKPKLLVLIGPTAVGKTKMSIEIAKEFGCEIISGDSMQVYRGMDIGTAKISEEEME 60 Query: 61 GIPHHLIDIHDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSE 120 G+PHHLIDIH+P YS AEFQEQ +LI EI+ +GKLPFIVGGTGLY+ES+CYG++FSE Sbjct: 61 GVPHHLIDIHEPDHPYSVAEFQEQCQRLIAEITERGKLPFIVGGTGLYVESVCYGYQFSE 120 Query: 121 AVADEAFRSEQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTL 180 ADE FR EQ +A +GAEALH +LAEVDPE+A RLHPND RR++RALE+ H T V L Sbjct: 121 TGADEVFREEQFRYANEYGAEALHQKLAEVDPETAERLHPNDLRRVVRALEVFHVTGVPL 180 Query: 181 SASHADQKKESPYELCLIGLTMDRKILYKRIEDRIDQMLADGLVAEVEGLLQRGYSRSLV 240 SA A Q K+SPY+LCL+GLTMDR++LY RIE+RID ML+ GLV EV L+ +G++ LV Sbjct: 181 SAQLAPQTKQSPYDLCLVGLTMDRQMLYNRIEERIDLMLSQGLVDEVAALMDKGFAPGLV 240 >B1DG22.1 B1DG22_9BACL tRNA delta(2)-isopentenylpyrophosphate transferase EC=2.5.1.8 Length = 335 Score = 323 bits (827), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 159/233 (68%), Positives = 189/233 (81%) Query: 4 ETRRRVLVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMMGIP 63 E ++ +LVL+GPTAVGKT+LSL+LA+A++AEIISGDSMQVY+ MDIGTAK+ E GI Sbjct: 8 ERKQPLLVLVGPTAVGKTQLSLDLAKAWNAEIISGDSMQVYKEMDIGTAKLPLAERQGIA 67 Query: 64 HHLIDIHDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSEAVA 123 HHLIDI +P AYS A+FQ + I +IS +GKLPFIVGGTGLY+ES+CYGF F+E + Sbjct: 68 HHLIDICEPDVAYSVADFQVHCKEAIADISARGKLPFIVGGTGLYVESVCYGFEFNENGS 127 Query: 124 DEAFRSEQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTLSAS 183 D AFR EQ+ +AE HGAEALHARLAEVDP+SA RLHPND+RRIIRALEI H + +SA Sbjct: 128 DPAFRDEQERYAEEHGAEALHARLAEVDPKSAERLHPNDQRRIIRALEIFHLSGEMMSAQ 187 Query: 184 HADQKKESPYELCLIGLTMDRKILYKRIEDRIDQMLADGLVAEVEGLLQRGYS 236 QKKESPYELC+IGLTMDRK+LY RIE R+DQML DGLV EV GLL RG + Sbjct: 188 LERQKKESPYELCIIGLTMDRKVLYSRIEQRVDQMLEDGLVEEVRGLLDRGVT 240 >C0ZEY7.1 C0ZEY7_BREBN tRNA delta(2)-isopentenylpyrophosphate transferase EC=2.5.1.8 Length = 319 Score = 282 bits (722), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 140/240 (58%), Positives = 176/240 (73%), Gaps = 1/240 (0%) Query: 1 LTTETRRRVLVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMM 60 +T + R R++V++GPTAVGKT+LSLELAE +D EIISGDSMQVYRGMDIGTAK +PEE+ Sbjct: 1 MTLQQRERLVVIIGPTAVGKTQLSLELAEQFDGEIISGDSMQVYRGMDIGTAKAEPEELA 60 Query: 61 GIPHHLIDIHDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSE 120 IPHHLIDI +P + YS A FQE LI +I+++G+LPFIVGGTGLYIES+ + F+FS Sbjct: 61 SIPHHLIDIKNPDEEYSVAMFQESAAGLISDINQRGRLPFIVGGTGLYIESVTHRFQFST 120 Query: 121 AVADEAFRSEQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTL 180 D R A++ G EALHARLA+VDP +A RLHPND +R+IRALEI+ + + Sbjct: 121 TSQDPELRDRLQRLADSEGVEALHARLADVDPITAERLHPNDVKRVIRALEIYESSGYKM 180 Query: 181 SASHADQKKESPYELCLIGLTMDRKILYKRIEDRIDQMLADGLVAEVEGLLQRGYSRSLV 240 S + K SPY+L +IGLTMDR LY+RI R++ M+ GLV EV GLL GY SLV Sbjct: 181 SDFQL-RAKHSPYDLVMIGLTMDRAKLYERINHRVELMIEAGLVEEVRGLLDAGYDASLV 239 >Q2B1N1.1 Q2B1N1_9BACI tRNA delta(2)-isopentenylpyrophosphate transferase EC=2.5.1.8 Length = 319 Score = 277 bits (709), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 134/231 (58%), Positives = 176/231 (76%) Query: 4 ETRRRVLVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMMGIP 63 +T+++++VL+GPTAVGKTRLS+ELA+ +DAEIISGDSMQ+Y+GMDIGTAKI EM GIP Sbjct: 11 DTKQKLIVLIGPTAVGKTRLSIELAKRFDAEIISGDSMQIYKGMDIGTAKITENEMDGIP 70 Query: 64 HHLIDIHDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSEAVA 123 HHLIDI P + +SAAEFQE K I I+ +GK+P IVGGTGLYI+S+ Y ++FSEA + Sbjct: 71 HHLIDIKAPNEPFSAAEFQELVRKEIACIASRGKMPMIVGGTGLYIQSVIYDYQFSEAPS 130 Query: 124 DEAFRSEQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTLSAS 183 D+AFR E + EA GAE+L L E+DP SA ++HPN+ RR+IRALE++H T T+S Sbjct: 131 DDAFRGELEKRLEAEGAESLFKELCEIDPASAEKMHPNNTRRVIRALEVYHCTGKTMSQY 190 Query: 184 HADQKKESPYELCLIGLTMDRKILYKRIEDRIDQMLADGLVAEVEGLLQRG 234 Q E Y+ +IGL+MDRK LY RI R+D+M+ DGL+ EVE L ++G Sbjct: 191 QEQQVPELLYDTSIIGLSMDRKKLYDRINSRVDKMMDDGLLEEVEALHRQG 241 >C2VFC7.1 C2VFC7_BACCE tRNA delta(2)-isopentenylpyrophosphate transferase Length = 317 Score = 269 bits (687), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 126/229 (55%), Positives = 170/229 (74%) Query: 6 RRRVLVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMMGIPHH 65 R +V V++GPTAVGKT+LS++LA+A + EIISGDSMQ+YR MDIGTAK+ EEM GIPH+ Sbjct: 6 REKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHY 65 Query: 66 LIDIHDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSEAVADE 125 ++DI DP+D++S AEFQE+ K I EI+ +GKLP IVGGTGLYI+S+ + ++F++ D Sbjct: 66 MVDIKDPEDSFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDA 125 Query: 126 AFRSEQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTLSASHA 185 +R + + A+ G E +H +L EVDPESA R+H N+ RR+IRALEI H T +S Sbjct: 126 IYREQMEKLAQERGVEYVHEKLQEVDPESAERIHANNVRRVIRALEIFHTTGEKMSDQLE 185 Query: 186 DQKKESPYELCLIGLTMDRKILYKRIEDRIDQMLADGLVAEVEGLLQRG 234 Q+ E Y++ LIGLTMDR++LY RI R+D M+ GL+ EVEGL RG Sbjct: 186 KQENELLYDVSLIGLTMDREMLYDRINLRVDLMMEQGLLEEVEGLYNRG 234 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,402,458,152 Number of extensions: 54982339 Number of successful extensions: 206504 Number of sequences better than 1.0e-05: 1764 Number of HSP's gapped: 203712 Number of HSP's successfully gapped: 1764 Length of query: 259 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 126 Effective length of database: 1,807,701,208 Effective search space: 227770352208 Effective search space used: 227770352208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)