BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0475.0007.p (476 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q3Y3U7.1 Q3Y3U7_ENTFC C4-dicarboxylate anaerobic carrier 572 e-161 C4G531.1 C4G531_ABIDE Putative uncharacterized protein 422 e-116 A4E7Q1.1 A4E7Q1_9ACTN Putative uncharacterized protein 421 e-115 Q184T7.1 Q184T7_CLOD6 Putative C4-dicarboxylate anaerobic carrier 391 e-107 Q186R3.1 Q186R3_CLOD6 Putative C4-dicarboxylate anaerobic carrier 390 e-106 >Q3Y3U7.1 Q3Y3U7_ENTFC C4-dicarboxylate anaerobic carrier Length = 466 Score = 572 bits (1474), Expect = e-161, Method: Compositional matrix adjust. Identities = 293/462 (63%), Positives = 355/462 (76%), Gaps = 8/462 (1%) Query: 1 METVKSKWKWSLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQEVVVDGSSRTAIIPGTYHT 60 MET+K K+ + PHSYT LTWI+PSGQF+ V G +T ++PGTYH Sbjct: 1 METMKRKF--TFPHSYTLIFILIILAAILTWIIPSGQFETVVENVAGMEKTTVVPGTYHQ 58 Query: 61 VPKISDQGDLRQGFAQIASAPMEGIINAADVVAFVLIVGGAFGIILQTGAIDRALMALAG 120 + D+ D QGF+ I AP EGII+A +VVAFVLIVGGAFGIIL+TGAIDR L LA Sbjct: 59 I----DKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKTGAIDRGLFTLAK 114 Query: 121 RLKTKGILLIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILILFLGTQ 180 L KGIL+IP++M++FSLGGSTFGMSEE+IPL+A+FI LMF+LG+DS+TAILILFL TQ Sbjct: 115 VLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGYDSMTAILILFLSTQ 174 Query: 181 IGYVGSTVNPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKKNPEKS 240 GYVGST+NPF+VLIAQGVAGV GNP LW R + W V TGIS+ FTM YA RVKKNP S Sbjct: 175 AGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIVFTMLYASRVKKNPLSS 234 Query: 241 AVFQNDILNRQHFIKENDGIEVKFSNRDKLIIGGFIVTLGIIVWGILSKGWYMTEIGALF 300 V+++D+ + F +N E +FS +DKLII GFIV LGI++WGI+SKGWYM EI ALF Sbjct: 235 IVYESDLKVKHKF--QNFDTEKEFSTQDKLIIAGFIVGLGIMIWGIISKGWYMVEISALF 292 Query: 301 FVLGIFSGIVTRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVVAENGMIIDTILNGLA 360 LG+F+G + R SQKEIAE FV+GC +F YAAVIIGLARG+LV+ E GMIIDTILN LA Sbjct: 293 LGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGVLVILEGGMIIDTILNSLA 352 Query: 361 TMLEGLPNYLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDLVGVNREAVVTAYQF 420 +L GLP +LFTTI+L+ +++F VPSSSG AALTMPV+APLGDL+ V+R+ VV + QF Sbjct: 353 NLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVMAPLGDLMNVSRDVVVLSNQF 412 Query: 421 GNGLTNLISPTGGVLLAGLAIARINFGQWLKVILKLFPILWL 462 GNGL N+ISPT GVLLAGLAIA INF +W KV K+F IL L Sbjct: 413 GNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFAILTL 454 >C4G531.1 C4G531_ABIDE Putative uncharacterized protein Length = 471 Score = 422 bits (1084), Expect = e-116, Method: Compositional matrix adjust. Identities = 210/460 (45%), Positives = 306/460 (66%), Gaps = 6/460 (1%) Query: 4 VKSKWKWSLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQEVVVDGSSRTAIIPGTYHTVPK 63 + K K LPH T +TWI+PSG F+R V R + GTYHTV K Sbjct: 1 MNEKKKLHLPHVITLIFVLIVFVAIMTWILPSGSFERTIVDTAAGEREVAVSGTYHTVEK 60 Query: 64 ISDQGDLRQGFAQIASAPMEGIINAADVVAFVLIVGGAFGIILQTGAIDRALMALAGRLK 123 +++ GDLRQG +Q+ AP +GI +V+AFV I+GG F I+ +TGA+ L L +L Sbjct: 61 VTEDGDLRQGLSQVLQAPGKGIQRGIEVIAFVFIIGGVFAIMDKTGAMTVGLKKLVKKLG 120 Query: 124 TKGILLIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILILFLGTQIGY 183 KGI+LIPI M +F LGGSTFGMS+E+ P Y I +P++ ++G+DS+T LI+ G +GY Sbjct: 121 NKGIILIPILMFLFGLGGSTFGMSDELAPFYLILMPIILSMGYDSVTVFLIVCGGATMGY 180 Query: 184 VGSTVNPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKKNPEKSAVF 243 ST+NPFSVLIAQGVAG+QGNPQL R I++ + I +GFTM+ A+R+KK+PEKS F Sbjct: 181 AASTINPFSVLIAQGVAGIQGNPQLIFRMIQFVIIMAIIIGFTMFRANRIKKHPEKSVTF 240 Query: 244 QNDILNRQHFIKENDGIEVK---FSNRDKLIIGGFIVTLGIIVWGILSKGWYMTEIGALF 300 +ND + R N I V F+ + K ++ FI L ++++G+++ GWYM E+ +F Sbjct: 241 ENDRIRRAAM---NANISVDNEVFTGKQKGVLLVFIAGLALVIFGLINFGWYMNELSMVF 297 Query: 301 FVLGIFSGIVTRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVVAENGMIIDTILNGLA 360 +GI GI+ +++++IAE F++G +AA++IG GI++VA++GMIIDTILN +A Sbjct: 298 LGMGIAMGIIGGLNERQIAEEFIEGVKGISFAAIVIGFCNGIMIVAQDGMIIDTILNAMA 357 Query: 361 TMLEGLPNYLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDLVGVNREAVVTAYQF 420 +LEG N++F M VV +++ LVPSSSG+AAL++P+++ L DL VN EA VT Q+ Sbjct: 358 NILEGATNFVFVGCMYVVQSLLALLVPSSSGQAALSIPIMSSLCDLHSVNPEASVTVLQY 417 Query: 421 GNGLTNLISPTGGVLLAGLAIARINFGQWLKVILKLFPIL 460 N +TN++SP G +AGLAI +I+FG+W K+ K + +L Sbjct: 418 ANQMTNIMSPVAGTTVAGLAICKISFGKWWKIFWKCWLVL 457 >A4E7Q1.1 A4E7Q1_9ACTN Putative uncharacterized protein Length = 487 Score = 421 bits (1081), Expect = e-115, Method: Compositional matrix adjust. Identities = 232/462 (50%), Positives = 314/462 (67%), Gaps = 6/462 (1%) Query: 2 ETVKSKWKWSLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQEVVVDGSSRTAIIPGTYHTV 61 + ++K+ LPH YT LTWIVPSGQ+ R+ + R + GTY V Sbjct: 7 KKTNRRFKFKLPHVYTIMFLLIVVFAVLTWIVPSGQYQRKTISTAAGEREVAVAGTYEQV 66 Query: 62 PK---ISDQGD---LRQGFAQIASAPMEGIINAADVVAFVLIVGGAFGIILQTGAIDRAL 115 K S+ G+ L Q + AP +GI AADVVAFVL++GG+F II +T A++ + Sbjct: 67 DKNYTDSETGETINLGQDIFAVLQAPTKGIQEAADVVAFVLLIGGSFAIITKTNALNAGM 126 Query: 116 MALAGRLKTKGILLIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILIL 175 + +LK K IL+IPI M + S+ G+TFGMSEE +P YAIFIP+M +G+DS+TA +I Sbjct: 127 SRVIKKLKNKDILIIPITMTLLSICGTTFGMSEEALPFYAIFIPIMMGIGYDSMTAFIIC 186 Query: 176 FLGTQIGYVGSTVNPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKK 235 FLG +GY ST++PF+VLIAQG+ G++GNPQLWLR + W +FT + + + M YA RVKK Sbjct: 187 FLGPNLGYCASTIDPFNVLIAQGIIGIEGNPQLWLRAVSWVIFTAVGIAWAMRYAMRVKK 246 Query: 236 NPEKSAVFQNDILNRQHFIKENDGIEVKFSNRDKLIIGGFIVTLGIIVWGILSKGWYMTE 295 NPE S V+++D L R F + IE +F+ R KL++ F +GIIVWG++++GWYM E Sbjct: 247 NPESSIVYEDDKLKRIEFSVTDASIEEEFTIRQKLVLIDFACGMGIIVWGLVTQGWYMNE 306 Query: 296 IGALFFVLGIFSGIVTRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVVAENGMIIDTI 355 I A+F +G+ +GI+ + Q+ IAE FVKG +F YAA++IG+ARGILV+AE GMIIDTI Sbjct: 307 ISAVFLGMGLLAGILGGLDQQTIAEEFVKGLADFAYAAIVIGIARGILVIAEGGMIIDTI 366 Query: 356 LNGLATMLEGLPNYLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDLVGVNREAVV 415 L LAT L G P ++TT M +V +++ LVPSSSG AALTMPV+ PL +L+G+N EA V Sbjct: 367 LQALATALAGAPAAVYTTFMYIVLGLLSLLVPSSSGLAALTMPVMGPLTELMGLNPEAAV 426 Query: 416 TAYQFGNGLTNLISPTGGVLLAGLAIARINFGQWLKVILKLF 457 TA QF N N ISP G+ +AGLA+ARI+FGQW K I K F Sbjct: 427 TALQFANQTINTISPVAGMTVAGLAVARISFGQWWKTIWKFF 468 >Q184T7.1 Q184T7_CLOD6 Putative C4-dicarboxylate anaerobic carrier Length = 468 Score = 391 bits (1005), Expect = e-107, Method: Compositional matrix adjust. Identities = 200/452 (44%), Positives = 300/452 (66%), Gaps = 14/452 (3%) Query: 10 WSLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQEVVVDGSSRTAIIPGTYHTVPKISDQGD 69 +++P + T LTWIVPSG F+R VD R+ ++ GTY P Sbjct: 14 FTMPQTLTIIFLLIVLMAILTWIVPSGNFER----VDIDGRSVVVAGTYEKAPSNP---- 65 Query: 70 LRQGFAQIASAPMEGIINAADVVAFVLIVGGAFGIILQTGAIDRALMALAGRLKTKGILL 129 QG + +AP+ G I+AA+VV FVLIVGGAFGI+ +TGAI+ + ++K L+ Sbjct: 66 --QGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEAVIAHTVNKMKKFQFLI 123 Query: 130 IPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILILFLGTQIGYVGSTVN 189 IPI+M++F LGG+TFGMSEE +P Y IFIPLM ++G+DS+TA+ +F+G G+ +T N Sbjct: 124 IPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTAVATVFIGATAGFGAATTN 183 Query: 190 PFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKKNPEKSAVFQNDI-L 248 PFSV IAQ ++ + + R + + ++ IS+GF M YA++VKK+P+KS V +DI L Sbjct: 184 PFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMYANKVKKDPKKSLV--HDISL 241 Query: 249 NRQHFIKENDGIEVKFSNRDKLIIGGFIVTLGIIVWGILSKGWYMTEIGALFFVLGIFSG 308 N++ + + I+ +F+ R+ ++I F + + I+++G+L WY+TEI +F +GI SG Sbjct: 242 NQELMVNSDTNIK-EFTKREAMVIAIFTIGMAIMIYGVLRLEWYITEIAMIFTAIGIISG 300 Query: 309 IVTRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVVAENGMIIDTILNGLATMLEGLPN 368 I + + Q EI +F+ G G+ I A + I ARGI+++AENG IIDTILN A +L GLP Sbjct: 301 IASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAENGFIIDTILNSAANLLNGLPK 360 Query: 369 YLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDLVGVNREAVVTAYQFGNGLTNLI 428 +F + ++ +I FL+PS+SG A+LT+PVLAPLGDLV V+R+ +VTAYQFG G+TNLI Sbjct: 361 TIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGDLVDVSRQMIVTAYQFGIGVTNLI 420 Query: 429 SPTGGVLLAGLAIARINFGQWLKVILKLFPIL 460 +PT GVL+ LA+A I + +W++ ++ L +L Sbjct: 421 TPTSGVLMGALAVANIPWSKWVRFVIPLMVVL 452 >Q186R3.1 Q186R3_CLOD6 Putative C4-dicarboxylate anaerobic carrier Length = 464 Score = 390 bits (1001), Expect = e-106, Method: Compositional matrix adjust. Identities = 209/459 (45%), Positives = 305/459 (66%), Gaps = 13/459 (2%) Query: 3 TVKSKWKWSLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQEVVVDGSSRTAIIPGTYHTVP 62 T K ++ +PH++T LTWIVPSG+F R++V R ++PGTY +V Sbjct: 2 TDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDV----EGRMVVVPGTYQSVE 57 Query: 63 KISDQGDLRQGFAQIASAPMEGIINAADVVAFVLIVGGAFGIILQTGAIDRALMALAGRL 122 QGFA + AP+EG I++A+VV FVLIVGGAFGI+ +TGAI+ + ++ + Sbjct: 58 SNP------QGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEAGIASVISKF 111 Query: 123 KTKGILLIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILILFLGTQIG 182 K K +L+IPI+M++F LGG+TFGM+EE +P Y IF+PLM +LG+DS+TAI I+F+G G Sbjct: 112 KGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTAIAIVFIGAAAG 171 Query: 183 YVGSTVNPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKKNPEKSAV 242 ST+NPFSV IAQ +A + + R + + + IS+ F M+YA +VK+NPE S V Sbjct: 172 AAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYYAKKVKENPESSIV 231 Query: 243 FQNDILNRQHFIKENDGIEVK-FSNRDKLIIGGFIVTLGIIVWGILSKGWYMTEIGALFF 301 ++ D N+ I E+K F+ ++ ++ F + + I++WG+LS+GWY+ EI F Sbjct: 232 YEIDKNNKS--ILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLSQGWYIPEISMCFA 289 Query: 302 VLGIFSGIVTRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVVAENGMIIDTILNGLAT 361 +G+ SG+V R+SQ EI+E+F+ G + AA+ I LARGI+++A+NG IIDTILN A Sbjct: 290 AIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIAQNGFIIDTILNSAAA 349 Query: 362 MLEGLPNYLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDLVGVNREAVVTAYQFG 421 L GLP +F + + + FL+PSSSG A+LT+PVLAPLGDLV ++ + +VTAYQFG Sbjct: 350 FLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLGDLVNISAQVIVTAYQFG 409 Query: 422 NGLTNLISPTGGVLLAGLAIARINFGQWLKVILKLFPIL 460 GLTN I+PT GVL+ LA+A I F +W++ I+ + IL Sbjct: 410 VGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLIL 448 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.143 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,738,335,676 Number of extensions: 119594055 Number of successful extensions: 494471 Number of sequences better than 1.0e-05: 679 Number of HSP's gapped: 492926 Number of HSP's successfully gapped: 695 Length of query: 476 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 336 Effective length of database: 1,741,754,103 Effective search space: 585229378608 Effective search space used: 585229378608 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)