BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0475.0004.p (270 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6M2S5.1 A6M2S5_CLOB8 PfkB domain protein 298 4e-79 Q3XZ07.1 Q3XZ07_ENTFC Carbohydrate kinase, PfkB 277 6e-73 C0D4D2.1 C0D4D2_9CLOT Putative uncharacterized protein 229 2e-58 C4FZX3.1 C4FZX3_ABIDE Putative uncharacterized protein 216 2e-54 A8RXQ2.1 A8RXQ2_9CLOT Putative uncharacterized protein 213 2e-53 >A6M2S5.1 A6M2S5_CLOB8 PfkB domain protein Length = 280 Score = 298 bits (763), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 145/279 (51%), Positives = 192/279 (68%), Gaps = 9/279 (3%) Query: 1 MKVLGIGDNVVDKYVHQRTMYPGGNALNFSVFAKGEGAEAAFLGVFGDDDEARLVENTLQ 60 +KV+G+GDNVVDKY H RTMYPGGNALNF+ +AK G +AFLG FG D EA V+NT+ Sbjct: 2 VKVIGLGDNVVDKYEHIRTMYPGGNALNFAAYAKKIGINSAFLGAFGSDAEADHVQNTIH 61 Query: 61 QLEIDISHCRHHSGENGCARVTLENGERIFLGSNEGGVTRLNPIMLSEADREYIRGFDLI 120 +L +DIS CRH +GENGCARVTLE+G+R+FLGSNEGGV R N + L++ D EYI+ FDL+ Sbjct: 62 ELGLDISKCRHFNGENGCARVTLEDGDRVFLGSNEGGVLRENGLNLTQDDLEYIKTFDLL 121 Query: 121 HTGLYSHTGHLLAELNELGIPLXXXXXXXXXXXQVERYIGNADFAFFSCSHMTEEETRNF 180 H GLY +T L ++ L IP+ +VER DF+FFSCSH+ +E N Sbjct: 122 HAGLYGYTEPELDKIRALSIPISFDFSDDFTIEEVERIAPKVDFSFFSCSHLKTDEMLNL 181 Query: 181 IQAKRRRDGQILVATRGGDSSIAYDGTAFYYQAPELIEAVDTMGAGDSYITAFLMSMI-- 238 + + +I+V T G ++ YDGT FY Q PEL+EAVDTMGAGDS+ITAFL++ I Sbjct: 182 VMKAHKLGSEIVVVTMGEKGALLYDGTKFYSQEPELVEAVDTMGAGDSFITAFLINYISK 241 Query: 239 -------KDGNIQAAMQEGCRLAAKSCMVEGSFGFGARY 270 ++ I+ ++ EG +AK+C+VEGSFG+G +Y Sbjct: 242 IKENELYRENIIKESLNEGAIFSAKTCLVEGSFGYGKKY 280 >Q3XZ07.1 Q3XZ07_ENTFC Carbohydrate kinase, PfkB Length = 271 Score = 277 bits (709), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 137/270 (50%), Positives = 179/270 (66%) Query: 1 MKVLGIGDNVVDKYVHQRTMYPGGNALNFSVFAKGEGAEAAFLGVFGDDDEARLVENTLQ 60 MKVLG+GDNVVDKY + MYPGGN+LNF+VFAK G E++F+GVFG D+EA V + ++ Sbjct: 1 MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIK 60 Query: 61 QLEIDISHCRHHSGENGCARVTLENGERIFLGSNEGGVTRLNPIMLSEADREYIRGFDLI 120 ++ +D SH R GENGCARV ++ G+RIFLGSN GGVTR PI L+E DREY+ F+LI Sbjct: 61 EIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELI 120 Query: 121 HTGLYSHTGHLLAELNELGIPLXXXXXXXXXXXQVERYIGNADFAFFSCSHMTEEETRNF 180 H GLYSH HLL EL + + +V+R I DF FFS ++EE + F Sbjct: 121 HMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQF 180 Query: 181 IQAKRRRDGQILVATRGGDSSIAYDGTAFYYQAPELIEAVDTMGAGDSYITAFLMSMIKD 240 + +L+ATRG ++I YDG FY P L E VDTM AGDS++TAFL+ ++ Sbjct: 181 LMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYFLEG 240 Query: 241 GNIQAAMQEGCRLAAKSCMVEGSFGFGARY 270 +I AM++G A KSC+V+GSFGFG Y Sbjct: 241 DDIVTAMKKGNEFAGKSCLVDGSFGFGIHY 270 >C0D4D2.1 C0D4D2_9CLOT Putative uncharacterized protein Length = 291 Score = 229 bits (585), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 118/279 (42%), Positives = 162/279 (58%), Gaps = 9/279 (3%) Query: 1 MKVLGIGDNVVDKYVHQRTMYPGGNALNFSVFAKGEGAEAAFLGVFGDDDEARLVENTLQ 60 MKV+G+GDNVVDKY H R MYPGGNA+NF+VFA+ G +AAFLG FGDD E V + + Sbjct: 13 MKVVGLGDNVVDKYEHIRVMYPGGNAMNFAVFARQLGVQAAFLGAFGDDPEGEHVAESAR 72 Query: 61 QLEIDISHCRHHSGENGCARVTLENGERIFLGSNEGGVTRLNPIMLSEADREYIRGFDLI 120 Q +DIS CR + GENGCARV LENG+R+F GSN GV R + L D EY+ GFDLI Sbjct: 73 QEGVDISRCRRYHGENGCARVRLENGDRVFQGSNRCGVLRTEGLRLEPEDYEYLLGFDLI 132 Query: 121 HTGLYSHTGHLLAELNELGIPLXXXXXXXXXXXQVERYIGNADFAFFSCSHMTEEETRNF 180 H+G++ + L E G + ++R + D+ FSCSH+ E Sbjct: 133 HSGIFGFAEDEVRTLKERGARISFDFSSSFTGEYLKRVLPYVDYPVFSCSHLEMGEMERL 192 Query: 181 IQAKRRRDGQILVATRGGDSSIAYDGTAFYYQAPELIEAVDTMGAGDSYITAFLMSMIKD 240 ++ + + + TRG + DG Y Q P L+EA DTM AGDS++T FL++ + + Sbjct: 193 LEYAAGQGCALTLCTRGESGAWLADGKEVYRQRPYLVEARDTMAAGDSFLTCFLVNYLNE 252 Query: 241 GN---------IQAAMQEGCRLAAKSCMVEGSFGFGARY 270 + +++ + AA CMV+GS+G G RY Sbjct: 253 SQKNPGGHEAAVTVSLERAAQFAANQCMVDGSWGHGVRY 291 >C4FZX3.1 C4FZX3_ABIDE Putative uncharacterized protein Length = 296 Score = 216 bits (549), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 117/295 (39%), Positives = 176/295 (59%), Gaps = 25/295 (8%) Query: 1 MKVLGIGDNVVDKYVHQRTMYPGGNALNFSVFAKGEGAEAAFLGVFGDDDEARLVENTLQ 60 ++VLG+GDNVVDKY+H RTMYPGGNALNF+V+AK G EA +LG+FGDD+ A V +T++ Sbjct: 2 LRVLGLGDNVVDKYMHIRTMYPGGNALNFAVYAKMFGIEAGYLGIFGDDEAAAHVCDTVR 61 Query: 61 QLEIDISHCRHHSGENGCARVTLENGERIFLGSNEGGVTRLNPIMLSEADREYIRGFDLI 120 + +D+SHCR + GENG A V L+NG+R+F+GSN+GGV++ + + L+E D++YI FD++ Sbjct: 62 NIGVDLSHCRFYRGENGYAEVRLDNGDRVFIGSNKGGVSKEHSLQLTELDKDYISKFDIV 121 Query: 121 HTGLYSHTGHLLAELNELGIPLXXXXXXXXXXXQVERYIGNADFAFFSCSHMTEEET-RN 179 HT ++S+ + L + + + +++ D A SC M E E R Sbjct: 122 HTSIFSYVENELPLIRAVSPFVSMDFSDRADEEYLKQCSPYIDCASISCGDMEENEIRRQ 181 Query: 180 FIQAKRRRDGQILVATRGGDSSIAYDGTAFYYQAPELIEAVDTMGAGDSYITAFLMSMI- 238 I+ K I++ATRG ++ FY Q+P L+EAVDTMGAGDS+IT FL++ + Sbjct: 182 MIRIKEFGCKHIVIATRGAAGALVMVDGHFYEQSPCLVEAVDTMGAGDSFITCFLINYVD 241 Query: 239 --KDGN---------------------IQAAMQEGCRLAAKSCMVEGSFGFGARY 270 KD +++ + +A +C +GSFGFG + Sbjct: 242 AMKDARDFPLASGTKGITTVTGYQELAVKSGLYRAAVYSAGNCQKDGSFGFGKVF 296 >A8RXQ2.1 A8RXQ2_9CLOT Putative uncharacterized protein Length = 311 Score = 213 bits (541), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 116/292 (39%), Positives = 171/292 (58%), Gaps = 25/292 (8%) Query: 1 MKVLGIGDNVVDKYVHQRTMYPGGNALNFSVFAKGEGAEAAFLGVFGDDDEARLVENTLQ 60 ++VLG+GDNVVDKY+H RTMYPGGNALNF+V+AK G EA +LGVFGDD+ A V +T++ Sbjct: 14 LRVLGLGDNVVDKYMHIRTMYPGGNALNFAVYAKMFGIEAGYLGVFGDDEAAAHVYDTIR 73 Query: 61 QLEIDISHCRHHSGENGCARVTLENGERIFLGSNEGGVTRLNPIMLSEADREYIRGFDLI 120 L +++SHCR + GENG A V L+NG+R+F+GSN+GGV+R +P+ L+ DR YI G+D++ Sbjct: 74 GLGLELSHCRFYPGENGYAEVRLDNGDRVFIGSNKGGVSREHPLELTAMDRAYIAGYDIV 133 Query: 121 HTGLYSHTGHLLAELNELGIPLXXXXXXXXXXXQVERYIGNADFAFFSCSHMTEEETRNF 180 HT ++S+ L L + + + + D A SC M E Sbjct: 134 HTSIFSYIEDELPLLRQASSFVSMDFSDRADEEYLRQCAPYVDCASISCGDMPRSEIEKQ 193 Query: 181 IQAKRRRDGQ-ILVATRGGDSSIAYDGTAFYYQAPELIEAVDTMGAGDSYITAFLMSMI- 238 + R+ + I++ATRG ++ Y Q+P L+EAVDTMGAGDS+IT FL++ + Sbjct: 194 MTLIRKFGCRHIVIATRGAKGALVMVDGRLYEQSPCLVEAVDTMGAGDSFITCFLINYVD 253 Query: 239 -----------------------KDGNIQAAMQEGCRLAAKSCMVEGSFGFG 267 +D ++ ++ +A +C +GSFGFG Sbjct: 254 AMKDSRDFPAASGTKGTVTAADYQDLAVRTSLYRAAVYSAGNCQKDGSFGFG 305 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,561,485,626 Number of extensions: 62646115 Number of successful extensions: 141361 Number of sequences better than 1.0e-05: 121 Number of HSP's gapped: 141268 Number of HSP's successfully gapped: 121 Length of query: 270 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 137 Effective length of database: 1,807,701,208 Effective search space: 247655065496 Effective search space used: 247655065496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)