BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0475.0003.p (321 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6M2S6.1 A6M2S6_CLOB8 Sugar isomerase (SIS) 437 e-121 Q3XZ08.1 Q3XZ08_ENTFC Sugar isomerase (SIS) 429 e-118 B5RGS5.1 B5RGS5_SALG2 Putative phosphosugar-binding protein 309 3e-82 B5R402.1 B5R402_SALEP Putative phosphosugar-binding protein 309 3e-82 B5FKM2.1 B5FKM2_SALDC Sugar isomerase 309 3e-82 >A6M2S6.1 A6M2S6_CLOB8 Sugar isomerase (SIS) Length = 322 Score = 437 bits (1125), Expect = e-121, Method: Compositional matrix adjust. Identities = 199/314 (63%), Positives = 254/314 (80%) Query: 1 MNVNQVVQSIREKVAEGISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFVHA 60 MN+ ++V IR +GI EV+ VACGGSLVDMY S+YFL SEA++L YT+NEFVHA Sbjct: 1 MNIREIVGKIRSSKEDGIKEVFLVACGGSLVDMYSSQYFLQSEARELRVLSYTSNEFVHA 60 Query: 61 LPKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLYEW 120 PK LG SVVI CSHGGNTPE+V AA+ A+E+GA TI LTHNK+A L+ Y+DY+ LYEW Sbjct: 61 TPKSLGKNSVVIACSHGGNTPETVEAAKVAREKGAATITLTHNKDADLIKYADYNILYEW 120 Query: 121 GDDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARVFA 180 G+D++V++NPMAIIL++AV++L+ E Y F +GL+ +N ++ +G+ VA RA+ +A Sbjct: 121 GNDTSVEDNPMAIILNIAVEILNQTEVYEGYNKFMEGLKIVNDVVKRGQGLVAERAKKYA 180 Query: 181 DKYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETPFI 240 +KY+NE + Y+L SGASYGHAYGF+ICSLMEMQW+NA+A+HSGEYFHGPFEVTDKET ++ Sbjct: 181 EKYKNEPITYVLGSGASYGHAYGFSICSLMEMQWMNASAVHSGEYFHGPFEVTDKETLYL 240 Query: 241 LMMNEGRTRALDERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSIICVYR 300 L+MNEGRTR LDERA FLN+Y KVEV+D KELGI I VVEFFNP++FYSI+CVYR Sbjct: 241 LLMNEGRTRELDERALKFLNKYGAKVEVVDCKELGISVIDSSVVEFFNPIVFYSILCVYR 300 Query: 301 EALAEVRNHPLETR 314 E LA++RNH L+TR Sbjct: 301 EELAKLRNHDLDTR 314 >Q3XZ08.1 Q3XZ08_ENTFC Sugar isomerase (SIS) Length = 322 Score = 429 bits (1102), Expect = e-118, Method: Compositional matrix adjust. Identities = 197/314 (62%), Positives = 249/314 (79%) Query: 1 MNVNQVVQSIREKVAEGISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFVHA 60 M+V + V +I EK IS +Y VACGGSLVDMY S YFL +EA K + GLYTANEF H Sbjct: 1 MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEASKCITGLYTANEFNHV 60 Query: 61 LPKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLYEW 120 PK+L +SVVI+CSHGGNT E+V A + A+ +GA T+GLTHNK A LL SD+SFLYEW Sbjct: 61 TPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTHNKTADLLKVSDHSFLYEW 120 Query: 121 GDDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARVFA 180 GDD+ + NNPMAIILDL V L++V E + H F++G++ I+ +I G KQV R +VFA Sbjct: 121 GDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKVIDTVIENGIKQVQDRIKVFA 180 Query: 181 DKYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETPFI 240 DKY++E ++YIL SG SYGHAYGF+ICSLMEMQWL++ ++HSGE+FHGPFEVTDK+T FI Sbjct: 181 DKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDSTSVHSGEFFHGPFEVTDKDTNFI 240 Query: 241 LMMNEGRTRALDERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSIICVYR 300 L+M+ GRTRALDERA+ FL++YAE+VE++DAKELG+ I+DEV EFFNP+LFYSI+ YR Sbjct: 241 LLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGLDMISDEVSEFFNPILFYSILSEYR 300 Query: 301 EALAEVRNHPLETR 314 ALA+ RNH L+ R Sbjct: 301 SALADARNHDLDVR 314 >B5RGS5.1 B5RGS5_SALG2 Putative phosphosugar-binding protein Length = 325 Score = 309 bits (791), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 152/316 (48%), Positives = 208/316 (65%), Gaps = 2/316 (0%) Query: 1 MNVNQVVQSIREKVAE--GISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFV 58 M + + V +I AE GI VY+VACGGS YP+K FL+ EAK L GLY + EF+ Sbjct: 2 MGMKETVSNIVTSQAEKGGIKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 61 Query: 59 HALPKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLY 118 + P LG +VV+V SH GNTPE++ AA+ A++ GA IGLT ++ L+ + DY Y Sbjct: 62 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWVMDSPLVAHCDYVETY 121 Query: 119 EWGDDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARV 178 +GD ++ L AV+LL EGYAH + FQ G+ IN I+ + +QVA RA+ Sbjct: 122 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 181 Query: 179 FADKYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETP 238 FA +Y+++++ Y + SGA YG AY +IC MEMQW+++A IHSGE+FHGPFE+TD TP Sbjct: 182 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 241 Query: 239 FILMMNEGRTRALDERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSIICV 298 F+ +EG TRA+DERA FL +Y ++EV+DAKELG+ TI V+++FN LF ++ V Sbjct: 242 FLFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 301 Query: 299 YREALAEVRNHPLETR 314 Y ALAE R HPL TR Sbjct: 302 YNRALAEARQHPLTTR 317 >B5R402.1 B5R402_SALEP Putative phosphosugar-binding protein Length = 325 Score = 309 bits (791), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 152/316 (48%), Positives = 208/316 (65%), Gaps = 2/316 (0%) Query: 1 MNVNQVVQSIREKVAE--GISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFV 58 M + + V +I AE GI VY+VACGGS YP+K FL+ EAK L GLY + EF+ Sbjct: 2 MGMKETVSNIVTSQAEKGGIKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 61 Query: 59 HALPKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLY 118 + P LG +VV+V SH GNTPE++ AA+ A++ GA IGLT ++ L+ + DY Y Sbjct: 62 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWVMDSPLVAHCDYVETY 121 Query: 119 EWGDDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARV 178 +GD ++ L AV+LL EGYAH + FQ G+ IN I+ + +QVA RA+ Sbjct: 122 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 181 Query: 179 FADKYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETP 238 FA +Y+++++ Y + SGA YG AY +IC MEMQW+++A IHSGE+FHGPFE+TD TP Sbjct: 182 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 241 Query: 239 FILMMNEGRTRALDERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSIICV 298 F+ +EG TRA+DERA FL +Y ++EV+DAKELG+ TI V+++FN LF ++ V Sbjct: 242 FLFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 301 Query: 299 YREALAEVRNHPLETR 314 Y ALAE R HPL TR Sbjct: 302 YNRALAEARQHPLTTR 317 >B5FKM2.1 B5FKM2_SALDC Sugar isomerase Length = 324 Score = 309 bits (791), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 152/316 (48%), Positives = 208/316 (65%), Gaps = 2/316 (0%) Query: 1 MNVNQVVQSIREKVAE--GISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFV 58 M + + V +I AE GI VY+VACGGS YP+K FL+ EAK L GLY + EF+ Sbjct: 1 MGMKETVSNIVTSQAEKGGIKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 60 Query: 59 HALPKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLY 118 + P LG +VV+V SH GNTPE++ AA+ A++ GA IGLT ++ L+ + DY Y Sbjct: 61 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWVMDSPLVAHCDYVETY 120 Query: 119 EWGDDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARV 178 +GD ++ L AV+LL EGYAH + FQ G+ IN I+ + +QVA RA+ Sbjct: 121 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 180 Query: 179 FADKYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETP 238 FA +Y+++++ Y + SGA YG AY +IC MEMQW+++A IHSGE+FHGPFE+TD TP Sbjct: 181 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 240 Query: 239 FILMMNEGRTRALDERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSIICV 298 F+ +EG TRA+DERA FL +Y ++EV+DAKELG+ TI V+++FN LF ++ V Sbjct: 241 FLFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 300 Query: 299 YREALAEVRNHPLETR 314 Y ALAE R HPL TR Sbjct: 301 YNRALAEARQHPLTTR 316 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,842,257,805 Number of extensions: 74216556 Number of successful extensions: 191416 Number of sequences better than 1.0e-05: 670 Number of HSP's gapped: 191730 Number of HSP's successfully gapped: 670 Length of query: 321 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 186 Effective length of database: 1,788,859,178 Effective search space: 332727807108 Effective search space used: 332727807108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)