BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0046.p (427 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DDL3.1 B1DDL3_9BACL Potassium uptake protein, TrkH family EC=3... 489 e-136 B3K6F0.1 B3K6F0_9BACI Potassium uptake protein, TrkH family EC=3... 480 e-133 B1DIM0.1 B1DIM0_9BACL Potassium uptake protein, TrkH family EC=3... 471 e-131 Q5L3I3.1 Q5L3I3_GEOKA K+ uptake transporter (Trk family) 431 e-119 C3J481.1 C3J481_9BACI Potassium uptake protein, TrkH family EC=3... 429 e-118 >B1DDL3.1 B1DDL3_9BACL Potassium uptake protein, TrkH family EC=3.6.3.14 Length = 446 Score = 489 bits (1260), Expect = e-136, Method: Compositional matrix adjust. Identities = 246/429 (57%), Positives = 314/429 (73%), Gaps = 7/429 (1%) Query: 2 VLIAAGTILLCLP-AASTGARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXX 60 ++I G +LL +P A+S G+R+ FIDALF ATSATCVTGL V+DTGT + FG+I Sbjct: 22 MIIVIGAVLLSMPIASSDGSRLPFIDALFTATSATCVTGLVVVDTGTYFSTFGKIVILSL 81 Query: 61 XXXXXXXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLS 120 TM+TLI LVL KRISLKERL+LQE+MNQ SM+GIVRLI+RV+ YSL I+L Sbjct: 82 IQIGGLGFMTMSTLIALVLRKRISLKERLILQEAMNQTSMEGIVRLIRRVIFYSLTIELV 141 Query: 121 GAILLAARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNIT 180 GA L A RF DM FG+A ++GLFHSIS FNNAGFD+FG F LT Y DP+VNI Sbjct: 142 GAALFAIRFAFDMPFGRAAWFGLFHSISFFNNAGFDIFGH----FRSLTMYVGDPLVNIV 197 Query: 181 SMLLIFLGGIGFIVLSDLIDYPKRKRLTLHSKVVLSASAVLILGGAAVFFWLELNA--TL 238 SMLLI GG+GF+V++DL+D+ K KRL+LHSKVVLS + +LI GA V F E + TL Sbjct: 198 SMLLIIFGGLGFVVMADLLDFRKNKRLSLHSKVVLSTTGILIAAGALVIFIFEFSNSNTL 257 Query: 239 KPLHAGGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKIT 298 LH G K++A+F Q++TPR+ G T++ LR++T F ++LMFIGA+PGSTGGGIK T Sbjct: 258 GSLHWGEKILAAFFQSVTPRTAGANTLDYTQLRQATVFFTVILMFIGASPGSTGGGIKTT 317 Query: 299 TFAILVVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLSVTESADF 358 TF +V IRG++DIV+F +R+ K+ +++A+T+T+LSL LV+ TMLLS TE F Sbjct: 318 TFTTMVGAVIAMIRGRDDIVLFHYRLGKDRIFKALTITMLSLTLVIFVTMLLSTTEDQQF 377 Query: 359 LTVLFEAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYALKPKNSKELYR 418 L +LFE SAFGT G+T+G+TP LT GK+++ + MF GR GPLTLAYAL PK +ELYR Sbjct: 378 LKILFETTSAFGTVGLTIGITPELTFFGKMVIALTMFAGRLGPLTLAYALGPKTERELYR 437 Query: 419 YPEGNITIG 427 YPEG ITIG Sbjct: 438 YPEGKITIG 446 >B3K6F0.1 B3K6F0_9BACI Potassium uptake protein, TrkH family EC=3.6.3.14 Length = 450 Score = 480 bits (1235), Expect = e-133, Method: Compositional matrix adjust. Identities = 253/424 (59%), Positives = 321/424 (75%), Gaps = 3/424 (0%) Query: 7 GTILLCLP-AASTGARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXXXXXXX 65 GT LL LP A + G +DALF A SA VTGL V+DTGT ++FGQI Sbjct: 27 GTFLLKLPIATADGTSTPLVDALFTAVSAISVTGLTVVDTGTHWSIFGQIVMLMLVQLGG 86 Query: 66 XXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLSGAILL 125 T AT I L+ N+RISL+ER++LQE+M Q +QGIV LI+RVL+Y+L+I+ +GA+LL Sbjct: 87 LGFMTSATWIALMFNRRISLRERMILQEAMGQYQIQGIVDLIRRVLVYTLIIEGAGALLL 146 Query: 126 AARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNITSMLLI 185 RF+ M A Y+G+F SISIFNNAGFDLFG IHGPFSG Y DP VNI M LI Sbjct: 147 TLRFSATMPLSDAAYFGIFQSISIFNNAGFDLFGQIHGPFSGFATYVSDPFVNIIVMFLI 206 Query: 186 FLGGIGFIVLSDLIDYPKRKRLTLHSKVVLSASAVLILGGAAVFFWLELN--ATLKPLHA 243 FLGGIGFIV+ DLI+YPK K+L+LHSKVVL+ +++LI+ GA + F LE + TL PL Sbjct: 207 FLGGIGFIVIFDLIEYPKCKKLSLHSKVVLTVTSLLIVIGAVMIFILEHSNPKTLGPLSY 266 Query: 244 GGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKITTFAIL 303 K+MAS Q+ITPRSGGV+T++I L +S+QF MILLMFIGAAPGSTGGGIK+TTFA+L Sbjct: 267 PVKIMASIFQSITPRSGGVSTLDIASLEQSSQFFMILLMFIGAAPGSTGGGIKVTTFAVL 326 Query: 304 VVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLSVTESADFLTVLF 363 + T I+GK D+V++R+RIS+ VYR+IT+T+LSL+L+V A+M LSVTES +FL +LF Sbjct: 327 IGAVVTMIQGKRDVVLYRNRISQALVYRSITLTILSLLLLVGASMFLSVTESGEFLRILF 386 Query: 364 EAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYALKPKNSKELYRYPEGN 423 EAVSAFGT+G++MGLT L+ +GK+ + +LMF+GR GPLTLAYAL KN+KELYR+PEG Sbjct: 387 EAVSAFGTAGLSMGLTTELSGVGKITIAMLMFLGRLGPLTLAYALSRKNNKELYRHPEGR 446 Query: 424 ITIG 427 ITIG Sbjct: 447 ITIG 450 >B1DIM0.1 B1DIM0_9BACL Potassium uptake protein, TrkH family EC=3.6.3.14 Length = 447 Score = 471 bits (1212), Expect = e-131, Method: Compositional matrix adjust. Identities = 243/428 (56%), Positives = 316/428 (73%), Gaps = 1/428 (0%) Query: 1 MVLIAAGTILLCLPAAST-GARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXX 59 +V+I G+ILL LP AS G FIDA F AT+A CVTGL V+DTGT + FG++ Sbjct: 20 IVIILIGSILLSLPYASEDGMHTPFIDAFFTATTAACVTGLVVVDTGTHFSTFGELVILM 79 Query: 60 XXXXXXXXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQL 119 TMATL L KRISL+ERL LQESM+ S++GIVR I+RVLIY+ I+ Sbjct: 80 LMQVGGLGFMTMATLFALAFRKRISLRERLWLQESMSHTSLEGIVRFIRRVLIYAFTIEF 139 Query: 120 SGAILLAARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNI 179 GA+LL R++ M F KA+YYG+FHS+S+F NAG D+ G IHGPF+ T + +DPI+N Sbjct: 140 VGALLLTLRWSAMMPFDKALYYGVFHSVSMFTNAGLDIMGPIHGPFNSFTNHVDDPIINA 199 Query: 180 TSMLLIFLGGIGFIVLSDLIDYPKRKRLTLHSKVVLSASAVLILGGAAVFFWLELNATLK 239 MLLIFLGGIGFIVL+++IDYPK ++L+LHSKVVL S +L LGGA + F E TL+ Sbjct: 200 VLMLLIFLGGIGFIVLAEVIDYPKIRKLSLHSKVVLYTSGLLFLGGAVLLFIFEYTNTLE 259 Query: 240 PLHAGGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKITT 299 PL GK+ +S Q+I+PRSGG T+ I +R ++QFL+I+LMFIGA+PGS GGGIK+TT Sbjct: 260 PLSFAGKLYSSLFQSISPRSGGANTLNIADMRNASQFLLIILMFIGASPGSAGGGIKVTT 319 Query: 300 FAILVVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLSVTESADFL 359 FAIL+ IRGKED+V F+ R++K+ V++A+T+T +S+ +V TM+LS TE DFL Sbjct: 320 FAILIGAVIAMIRGKEDVVFFKRRLAKDRVHKALTITAVSVATIVLFTMILSATEETDFL 379 Query: 360 TVLFEAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYALKPKNSKELYRY 419 TV+FE SAFGT+G++MGLTP+LT IGK+L+II+MF+GR GPLTLAY L P SKELYR+ Sbjct: 380 TVMFEVTSAFGTTGLSMGLTPHLTGIGKILIIIVMFMGRMGPLTLAYTLVPNQSKELYRH 439 Query: 420 PEGNITIG 427 P+G ITIG Sbjct: 440 PKGKITIG 447 >Q5L3I3.1 Q5L3I3_GEOKA K+ uptake transporter (Trk family) Length = 445 Score = 431 bits (1107), Expect = e-119, Method: Compositional matrix adjust. Identities = 219/430 (50%), Positives = 306/430 (71%), Gaps = 8/430 (1%) Query: 2 VLIAAGTILLCLPAAST-GARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXX 60 ++I G LL LP A+ G + F+DALF ATSATCVTGL V+DTGT T+FGQ+ Sbjct: 20 LIILVGAFLLMLPTATVDGKGLPFLDALFTATSATCVTGLVVVDTGTTFTLFGQLVILAL 79 Query: 61 XXXXXXXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLS 120 T ATL +L KRISLKERLLLQE++NQ S++GIVRL++R+L++++VI+ Sbjct: 80 IQVGGLGFMTFATLFAFLLGKRISLKERLLLQEALNQLSIEGIVRLVKRILVFTVVIEGI 139 Query: 121 GAILLAARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNIT 180 G +LLA RF+ DM F KAVY+G FH+IS FNNAGFDL G+ F LT Y +DP+V++ Sbjct: 140 GGLLLAIRFSFDMPFWKAVYFGFFHAISNFNNAGFDLMGE----FRSLTGYVDDPVVSLV 195 Query: 181 SMLLIFLGGIGFIVLSDLIDYPKRKRLTLHSKVVLSASAVLILGGAAVFFWLELN--ATL 238 +LI LGGIGFIV++++ +Y + RL+LH+KVV +A L++ A+ LE N T+ Sbjct: 196 VPILIILGGIGFIVMNEVYEYRQTSRLSLHTKVVAITTAWLLVASMALILLLEWNNPKTM 255 Query: 239 KPLHAGGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKIT 298 PL GK +++F QA+TPR+ G T+ IP L + T FL++ LMF+GA+PGSTGGGIK T Sbjct: 256 GPLSLSGKFLSAFYQAVTPRTAGSNTLNIPDLTQPTLFLIVFLMFVGASPGSTGGGIKTT 315 Query: 299 TFAILVVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLSVTESA-D 357 TF L+ +++IRGKED+++FR RI + VY+++T+T+ L +V+ TM+L++TES D Sbjct: 316 TFTTLLGAVWSQIRGKEDVILFRKRIVYDTVYKSLTVTMSGLFIVMFVTMVLTITESGKD 375 Query: 358 FLTVLFEAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYALKPKNSKELY 417 FL +LFEA SAFGT G++MGLTP L+ +GK ++I+ MF GR GPLT+AYA+ + + + Sbjct: 376 FLMILFEATSAFGTVGLSMGLTPELSPLGKAVIILTMFAGRVGPLTIAYAVTLRRQPDPF 435 Query: 418 RYPEGNITIG 427 RYP+G I IG Sbjct: 436 RYPKGKIMIG 445 >C3J481.1 C3J481_9BACI Potassium uptake protein, TrkH family EC=3.6.3.14 Length = 445 Score = 429 bits (1103), Expect = e-118, Method: Compositional matrix adjust. Identities = 217/430 (50%), Positives = 304/430 (70%), Gaps = 8/430 (1%) Query: 2 VLIAAGTILLCLPAAST-GARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXX 60 ++I G LL LP A+ G + F+DALF ATSATCVTGL V+DTGT T+FGQ+ Sbjct: 20 LIILVGAFLLMLPTATVDGKGLPFLDALFTATSATCVTGLVVVDTGTTFTLFGQLVILAL 79 Query: 61 XXXXXXXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLS 120 T ATL +L KRISLKERLLLQE++NQ S++GIVRL++R+L++++VI+ Sbjct: 80 IQVGGLGFMTFATLFAFLLGKRISLKERLLLQEALNQLSIEGIVRLVKRILVFTVVIEGI 139 Query: 121 GAILLAARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNIT 180 G +LLA RF+ DM F KAVY+G FH+IS FNNAGFDL G+ F LT Y EDP+V++ Sbjct: 140 GGLLLAIRFSFDMPFWKAVYFGFFHAISNFNNAGFDLMGE----FRSLTGYVEDPVVSLV 195 Query: 181 SMLLIFLGGIGFIVLSDLIDYPKRKRLTLHSKVVLSASAVLILGGAAVFFWLELN--ATL 238 +LI LGGIGFIV++++ +Y + +RL+LH+KVV+ +A L+ A+ LE N T+ Sbjct: 196 VPILIILGGIGFIVMNEVYEYRQTRRLSLHTKVVVITTAWLVFASMALILLLEWNNPKTM 255 Query: 239 KPLHAGGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKIT 298 PL GK +++F QA+TPR+ G T+ IP L + T FL++ LMF+GA+PGSTGGGIK T Sbjct: 256 GPLSLSGKFLSAFYQAVTPRTAGSNTLNIPDLTQPTLFLIVFLMFVGASPGSTGGGIKTT 315 Query: 299 TFAILVVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLSVTESA-D 357 TF L+ +++IRGKED+++FR RI + VY+++T+T+ L +V+ TM+L++TE D Sbjct: 316 TFTTLLGAVWSQIRGKEDVILFRKRIVYDTVYKSLTVTMSGLFVVMLVTMVLTITEKGKD 375 Query: 358 FLTVLFEAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYALKPKNSKELY 417 F +LFEA SAFGT G++MGLTP L+ +GK ++I MF GR GPLT+AYA+ + + + Sbjct: 376 FFMILFEATSAFGTVGLSMGLTPELSPLGKAIIIFTMFAGRVGPLTIAYAVTLRRQPDPF 435 Query: 418 RYPEGNITIG 427 RYP+G + IG Sbjct: 436 RYPKGKMMIG 445 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.140 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,117,132,370 Number of extensions: 78993303 Number of successful extensions: 306449 Number of sequences better than 1.0e-05: 1049 Number of HSP's gapped: 302209 Number of HSP's successfully gapped: 1052 Length of query: 427 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 288 Effective length of database: 1,751,175,118 Effective search space: 504338433984 Effective search space used: 504338433984 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)