BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0020.p (204 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D8L3.1 B1D8L3_9BACL SNARE associated Golgi protein 251 2e-65 B8DE10.1 B8DE10_LISMH DedA family protein 224 3e-57 Q8Y724.1 Q8Y724_LISMO Lmo1500 protein 223 6e-57 Q4EU09.1 Q4EU09_LISMO DedA family protein 223 6e-57 Q71ZH0.1 Q71ZH0_LISMF DedA family protein 223 7e-57 >B1D8L3.1 B1D8L3_9BACL SNARE associated Golgi protein Length = 203 Score = 251 bits (642), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 124/202 (61%), Positives = 155/202 (76%) Query: 1 MHQLLNAITDAALNLVSSLGIWGIWIGMVLESACIPIPSEVIMLSGGLLIARGTLSFPEV 60 MH +++ I++ A+ L+ ++GI+GI+IGMVLESACIPIPSEVI+LSGGL A GT+S+ V Sbjct: 1 MHSVIDWISNIAVQLIDAVGIYGIFIGMVLESACIPIPSEVILLSGGLAAANGTISWQAV 60 Query: 61 VAAGVLGNLMGSIAAFYVGMHGGRKLLEKYGKYILFNSRHLEQSQRWFDRYGESTVFFTR 120 V AGVLGN+ GSI A+YVG GGRK+L KYGKY+ F + HL Q++RWF RYG S+VFF R Sbjct: 61 VIAGVLGNVAGSIIAYYVGALGGRKVLNKYGKYVFFRNSHLTQAERWFTRYGASSVFFAR 120 Query: 121 MLPFIRTFISLPAGIAGMKTGKFIFFTALGSLPWNIALVYLGYRLGDNWTIVEKYMRPVS 180 LPFIRTFISLPAGIA MK KF+ FT LG +PWN+AL Y GY+ G N ++EKYM PVS Sbjct: 121 NLPFIRTFISLPAGIAEMKFSKFLLFTFLGCIPWNVALAYAGYKGGANIDLLEKYMHPVS 180 Query: 181 YAICGAVVILIILWLVRRKKER 202 YAI V ++ WL RK+ R Sbjct: 181 YAIALLVAGGLVYWLFLRKRIR 202 >B8DE10.1 B8DE10_LISMH DedA family protein Length = 208 Score = 224 bits (572), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 112/206 (54%), Positives = 149/206 (72%), Gaps = 4/206 (1%) Query: 1 MHQLLNAITDAALNLVSSLGIWGIWIGMVLESACIPIPSEVIMLSGGLLIARGTLSFPEV 60 +H L+ ++ D + LV SLG WGI++GM++ES CIP+PSEVIML GG + G L+F V Sbjct: 2 LHTLIQSLQDFTMWLVDSLGHWGIFLGMLIESVCIPLPSEVIMLFGGFMAEAGKLNFWLV 61 Query: 61 VAAGVLGNLMGSIAAFYVGMHGGRKLLEKYGKYILFNSRHLEQSQRWFDRYGESTVFFTR 120 V AG+ GNL+GS+ A+Y+G GGR L+ KYGKYI N +HL++++ WF RYG VFF R Sbjct: 62 VFAGISGNLVGSLIAYYIGKFGGRALVLKYGKYIFLNVKHLDKAELWFGRYGARAVFFGR 121 Query: 121 MLPFIRTFISLPAGIAGMKTGKFIFFTALGSLPWNIALVYLGYRLGDNWTIVEKYMRPVS 180 +LP IRTFISLPAGIA M KFI +T LG +PWNI L +LGY LG NW++VEKY RP+S Sbjct: 122 VLPVIRTFISLPAGIAKMNVWKFIIYTILGCIPWNIFLTWLGYSLGSNWSVVEKYTRPIS 181 Query: 181 YAICGAVVILIILWLVRR---KKERT 203 Y + A+VI I+++L + K+ R Sbjct: 182 YLML-ALVIAIVVYLAYKIWNKRARN 206 >Q8Y724.1 Q8Y724_LISMO Lmo1500 protein Length = 208 Score = 223 bits (569), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 112/206 (54%), Positives = 149/206 (72%), Gaps = 4/206 (1%) Query: 1 MHQLLNAITDAALNLVSSLGIWGIWIGMVLESACIPIPSEVIMLSGGLLIARGTLSFPEV 60 +H L+ ++ D + LV SLG WGI++GM++ES CIP+PSEVIML GG + G L+F V Sbjct: 2 LHTLIQSLQDFTMWLVDSLGHWGIFLGMLIESVCIPLPSEVIMLFGGFMAEAGKLNFWLV 61 Query: 61 VAAGVLGNLMGSIAAFYVGMHGGRKLLEKYGKYILFNSRHLEQSQRWFDRYGESTVFFTR 120 V AG+ GNL+GS+ A+Y+G GGR L+ K+GKYI N +HL++++ WF RYG VFF R Sbjct: 62 VFAGISGNLVGSLIAYYIGKFGGRALVLKFGKYIFLNVKHLDKAELWFGRYGARAVFFGR 121 Query: 121 MLPFIRTFISLPAGIAGMKTGKFIFFTALGSLPWNIALVYLGYRLGDNWTIVEKYMRPVS 180 +LP IRTFISLPAGIA M KFI +T LG +PWNI L +LGY LG NW++VEKY RP+S Sbjct: 122 VLPVIRTFISLPAGIAKMNVWKFIIYTILGCIPWNIFLTWLGYSLGSNWSVVEKYTRPIS 181 Query: 181 YAICGAVVILIILWL---VRRKKERT 203 Y + A+VI I+++L V K+ R Sbjct: 182 YLML-ALVIAIVVYLAYKVWNKRARN 206 >Q4EU09.1 Q4EU09_LISMO DedA family protein Length = 208 Score = 223 bits (569), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 112/206 (54%), Positives = 149/206 (72%), Gaps = 4/206 (1%) Query: 1 MHQLLNAITDAALNLVSSLGIWGIWIGMVLESACIPIPSEVIMLSGGLLIARGTLSFPEV 60 +H L+ ++ D + LV SLG WGI++GM++ES CIP+PSEVIML GG + G L+F V Sbjct: 2 LHTLIQSLQDFTMWLVDSLGHWGIFLGMLIESVCIPLPSEVIMLFGGFMAEAGKLNFWLV 61 Query: 61 VAAGVLGNLMGSIAAFYVGMHGGRKLLEKYGKYILFNSRHLEQSQRWFDRYGESTVFFTR 120 V AG+ GNL+GS+ A+Y+G GGR L+ K+GKYI N +HL++++ WF RYG VFF R Sbjct: 62 VFAGISGNLVGSLIAYYIGKFGGRALVLKFGKYIFLNVKHLDKAELWFGRYGARAVFFGR 121 Query: 121 MLPFIRTFISLPAGIAGMKTGKFIFFTALGSLPWNIALVYLGYRLGDNWTIVEKYMRPVS 180 +LP IRTFISLPAGIA M KFI +T LG +PWNI L +LGY LG NW++VEKY RP+S Sbjct: 122 VLPVIRTFISLPAGIAKMNVWKFIIYTILGCIPWNIFLTWLGYSLGSNWSVVEKYTRPIS 181 Query: 181 YAICGAVVILIILWL---VRRKKERT 203 Y + A+VI I+++L V K+ R Sbjct: 182 YLML-ALVIAIVVYLAYKVWNKRARN 206 >Q71ZH0.1 Q71ZH0_LISMF DedA family protein Length = 208 Score = 223 bits (568), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 111/206 (53%), Positives = 149/206 (72%), Gaps = 4/206 (1%) Query: 1 MHQLLNAITDAALNLVSSLGIWGIWIGMVLESACIPIPSEVIMLSGGLLIARGTLSFPEV 60 +H L+ ++ D + LV SLG WGI++GM++ES CIP+PSEVIML GG + G L+F V Sbjct: 2 LHTLIQSLQDFTMWLVDSLGHWGIFLGMLIESVCIPLPSEVIMLFGGFMAEAGKLNFWLV 61 Query: 61 VAAGVLGNLMGSIAAFYVGMHGGRKLLEKYGKYILFNSRHLEQSQRWFDRYGESTVFFTR 120 V AG+ GNL+GS+ A+Y+G GGR L+ K+GKYI N +HL++++ WF RYG VFF R Sbjct: 62 VFAGIAGNLVGSLIAYYIGKFGGRALVLKFGKYIFLNVKHLDKAELWFGRYGARAVFFGR 121 Query: 121 MLPFIRTFISLPAGIAGMKTGKFIFFTALGSLPWNIALVYLGYRLGDNWTIVEKYMRPVS 180 +LP IRTFISLPAGIA M KF+ +T LG +PWNI L +LGY LG NW++VEKY RP+S Sbjct: 122 VLPVIRTFISLPAGIAKMNVWKFVIYTILGCIPWNIFLTWLGYSLGSNWSVVEKYTRPIS 181 Query: 181 YAICGAVVILIILWL---VRRKKERT 203 Y + A+VI I+++L V K+ R Sbjct: 182 YLML-ALVIAIVVYLAYKVWNKRARN 206 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.143 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,219,283,911 Number of extensions: 50177893 Number of successful extensions: 190312 Number of sequences better than 1.0e-05: 2507 Number of HSP's gapped: 189955 Number of HSP's successfully gapped: 2507 Length of query: 204 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 75 Effective length of database: 1,845,385,268 Effective search space: 138403895100 Effective search space used: 138403895100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 128 (53.9 bits)