BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0017.p (432 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9Q1.1 B3K9Q1_9BACI Glycoside hydrolase family 4 792 0.0 B1DIG9.1 B1DIG9_9BACL Glycoside hydrolase family 4 756 0.0 C0ZDC9.1 C0ZDC9_BREBN Alpha-galactosidase EC=3.2.1.22 702 0.0 B7DMX2.1 B7DMX2_9BACL Glycoside hydrolase family 4 698 0.0 C1PQI8.1 C1PQI8_ALIAC Alpha-galactosidase EC=3.2.1.22 697 0.0 >B3K9Q1.1 B3K9Q1_9BACI Glycoside hydrolase family 4 Length = 432 Score = 792 bits (2045), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/432 (86%), Positives = 399/432 (92%) Query: 1 MNKITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGST 60 M+KITFIGAGSTVFAKNVLGDCM T ALQGFE ALFDID RL+DS MLNNIK ++GST Sbjct: 1 MSKITFIGAGSTVFAKNVLGDCMQTPALQGFELALFDIDPVRLKDSENMLNNIKKTSGST 60 Query: 61 CIVKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN 120 C+VK+Y DRKEALRGAKYVINAIQVGGYDPCTITDFE+PKKYGLRQTIADTVGIGGIFRN Sbjct: 61 CVVKSYGDRKEALRGAKYVINAIQVGGYDPCTITDFEVPKKYGLRQTIADTVGIGGIFRN 120 Query: 121 LRTIPVMLDFAADIREVCPDALFLNYTNPMAVLTNVMNTYGGVQTVGLCHSVQHCIPGLF 180 LRTIPVMLDFAAD+REVCP+ALFLNYTNPMAVLTN MNT GGVQTVGLCHSVQ CIP LF Sbjct: 121 LRTIPVMLDFAADMREVCPNALFLNYTNPMAVLTNAMNTLGGVQTVGLCHSVQACIPHLF 180 Query: 181 EALGMDQTGVQAKIAGINHMAWLLEVTKDGEDLYPEIKRRAAEKQKEHHHDMVRYEMMLK 240 + LG+D+ GVQAKIAGINHMAWLLEVTKDG DLYPEIK+RAAEKQKE HHDMVR EMML Sbjct: 181 DNLGIDKEGVQAKIAGINHMAWLLEVTKDGVDLYPEIKKRAAEKQKEKHHDMVRLEMMLN 240 Query: 241 FGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWKQMREELVHDT 300 FGYYITESSEHNAEYHPYFIKRNYPELIERF IPLDEYPRRCV QI WK MRE+LV++ Sbjct: 241 FGYYITESSEHNAEYHPYFIKRNYPELIERFNIPLDEYPRRCVNQIEGWKAMREDLVNNQ 300 Query: 301 TLHHERSHEYASYILEAIETNVPFKIGGNVLNTGLITNLPREACVEVPCLVDASGVTPTY 360 L H RSHEYASYILEAIETNVPFKIGGNV+NTGLITNLP+EACVEVPCLVDASGVTPTY Sbjct: 301 DLTHNRSHEYASYILEAIETNVPFKIGGNVMNTGLITNLPKEACVEVPCLVDASGVTPTY 360 Query: 361 VGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSMDDIVAMCDEL 420 VGDLPPQCAALNRTNINTQLLT+EAA TGKKEHIYHAAMLDPHT+AELSMDDI ++CD+L Sbjct: 361 VGDLPPQCAALNRTNINTQLLTLEAAATGKKEHIYHAAMLDPHTAAELSMDDIKSLCDDL 420 Query: 421 IAAHGQWLPDFK 432 I AHG WLP ++ Sbjct: 421 IEAHGDWLPKYQ 432 >B1DIG9.1 B1DIG9_9BACL Glycoside hydrolase family 4 Length = 432 Score = 756 bits (1952), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/432 (81%), Positives = 389/432 (90%) Query: 1 MNKITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGST 60 M+KITF+GAGSTVFAKNVLGD M T LQGFE ALFDID RL DS ++L N+K S GS Sbjct: 1 MSKITFLGAGSTVFAKNVLGDVMLTEPLQGFELALFDIDAGRLRDSEQLLLNMKQSLGSN 60 Query: 61 CIVKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN 120 C+VKAY+DRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN Sbjct: 61 CVVKAYSDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN 120 Query: 121 LRTIPVMLDFAADIREVCPDALFLNYTNPMAVLTNVMNTYGGVQTVGLCHSVQHCIPGLF 180 LRTIPV+LDFA D+REVCPDALF+NYTNPMAVLTNV++T GGV+TVGLCHSVQ C+ LF Sbjct: 121 LRTIPVVLDFAKDMREVCPDALFMNYTNPMAVLTNVLSTAGGVRTVGLCHSVQACVSDLF 180 Query: 181 EALGMDQTGVQAKIAGINHMAWLLEVTKDGEDLYPEIKRRAAEKQKEHHHDMVRYEMMLK 240 ++L MD TGV++KIAGINHMAWLLEV KDG DLYPEIKRRAAEKQKE HHDMVR+EMMLK Sbjct: 181 KSLEMDATGVESKIAGINHMAWLLEVKKDGVDLYPEIKRRAAEKQKEKHHDMVRFEMMLK 240 Query: 241 FGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWKQMREELVHDT 300 FGYYITESSEHNAEYHPYFIKRNYPEL++RF IPLDEYPRRC+EQI W MRE L+ ++ Sbjct: 241 FGYYITESSEHNAEYHPYFIKRNYPELVDRFNIPLDEYPRRCIEQIKGWNSMRESLLANS 300 Query: 301 TLHHERSHEYASYILEAIETNVPFKIGGNVLNTGLITNLPREACVEVPCLVDASGVTPTY 360 + H+RSHEYAS+I+EAIET FKIGGNV+NTGLITNLP+EACVEVPCLVD SG+ PTY Sbjct: 301 NIEHKRSHEYASHIMEAIETGKTFKIGGNVMNTGLITNLPKEACVEVPCLVDRSGINPTY 360 Query: 361 VGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSMDDIVAMCDEL 420 VGDLPPQ AALNRTNINTQLLTIEAA T KKEHIYHAAMLDPHTSAELSMDDIVA+CD+L Sbjct: 361 VGDLPPQLAALNRTNINTQLLTIEAALTQKKEHIYHAAMLDPHTSAELSMDDIVALCDDL 420 Query: 421 IAAHGQWLPDFK 432 I AHG WLP +K Sbjct: 421 IEAHGDWLPAYK 432 >C0ZDC9.1 C0ZDC9_BREBN Alpha-galactosidase EC=3.2.1.22 Length = 433 Score = 702 bits (1812), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/432 (76%), Positives = 377/432 (87%), Gaps = 1/432 (0%) Query: 1 MNKITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGST 60 M KITF+GAGSTVFAKNVLGDCM +AL+G E AL+DID +RL+DS MLNN+K STG Sbjct: 1 MPKITFLGAGSTVFAKNVLGDCMHVSALEGSEFALYDIDPQRLKDSESMLNNLKESTGRE 60 Query: 61 CIVKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN 120 VKAYTDRKEALRGAKYVINA+Q+GGYDPCTITDFEIPK+YGL+QTIADTVGIGGIFRN Sbjct: 61 VTVKAYTDRKEALRGAKYVINAVQIGGYDPCTITDFEIPKRYGLQQTIADTVGIGGIFRN 120 Query: 121 LRTIPVMLDFAADIREVCPDALFLNYTNPMAVLTNVMNTYGGVQTVGLCHSVQHCIPGLF 180 LRTIPV+LDFA D+ EVCP+A FLNYTNPMA LT + +GG++ VGLCHSVQ C P L Sbjct: 121 LRTIPVLLDFARDMEEVCPNAWFLNYTNPMAALTGTLLRFGGIKAVGLCHSVQVCAPYLL 180 Query: 181 EALGMDQTGVQAKIAGINHMAWLLEVTKDGEDLYPEIKRRAAEKQKEHHHDMVRYEMMLK 240 ++LGM+ +Q KIAGINHMAWLLE+T+DG DLYPEIKRRAAEKQK H DMVR+E+ML+ Sbjct: 181 KSLGMETDNLQYKIAGINHMAWLLEITRDGVDLYPEIKRRAAEKQKARHDDMVRFELMLR 240 Query: 241 FGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWKQMREELVHDT 300 FGYY+TESSEHNAEYHPYFIK YPELIERF IPLDEYPRRCVEQI+KW MRE+LV++ Sbjct: 241 FGYYVTESSEHNAEYHPYFIKGRYPELIERFHIPLDEYPRRCVEQINKWTAMREDLVNNK 300 Query: 301 TLHHERSHEYASYILEAIETNVPFKIGGNVLNTG-LITNLPREACVEVPCLVDASGVTPT 359 + H R+ EYASYI+EA+ETN PF IGGNVLNTG LI+NLP +ACVEVPCLVD +GVTPT Sbjct: 301 QISHARTLEYASYIIEAMETNKPFTIGGNVLNTGRLISNLPEKACVEVPCLVDGNGVTPT 360 Query: 360 YVGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSMDDIVAMCDE 419 YVG+LP Q AALNRTNINTQLLTIEAA T KKEHIYHAA+LDPHT++ELS+DDIV++CD+ Sbjct: 361 YVGELPEQLAALNRTNINTQLLTIEAAITRKKEHIYHAALLDPHTASELSIDDIVSLCDD 420 Query: 420 LIAAHGQWLPDF 431 LI AHG+WLP F Sbjct: 421 LIEAHGEWLPQF 432 >B7DMX2.1 B7DMX2_9BACL Glycoside hydrolase family 4 Length = 432 Score = 698 bits (1801), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/431 (75%), Positives = 371/431 (86%) Query: 1 MNKITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGST 60 M+KI FIGAGSTVFAKNVLGDCM T +L+GFE ALFDID ERL+DS RML N+K + Sbjct: 1 MDKIAFIGAGSTVFAKNVLGDCMLTPSLRGFEFALFDIDEERLKDSERMLLNLKEQLRAD 60 Query: 61 CIVKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN 120 ++AY DRK+AL GAKYVINAIQVGGY+PCT+ DFE+PKKYGLRQTI DTVGIGGIFR Sbjct: 61 VHIQAYRDRKQALTGAKYVINAIQVGGYEPCTVIDFEVPKKYGLRQTIGDTVGIGGIFRA 120 Query: 121 LRTIPVMLDFAADIREVCPDALFLNYTNPMAVLTNVMNTYGGVQTVGLCHSVQHCIPGLF 180 LRTIPVML A D++EVCPDA FLNYTNPMAVLTN+M T+GG++TVGLCHSVQ C+P LF Sbjct: 121 LRTIPVMLSIAEDMQEVCPDAWFLNYTNPMAVLTNIMITHGGIKTVGLCHSVQVCVPNLF 180 Query: 181 EALGMDQTGVQAKIAGINHMAWLLEVTKDGEDLYPEIKRRAAEKQKEHHHDMVRYEMMLK 240 +L MD GV+ KIAGINHMAWLLEV KDG DLYPEIKRRA EKQK+ H DMVR+E+M K Sbjct: 181 RSLNMDPEGVEYKIAGINHMAWLLEVKKDGVDLYPEIKRRAREKQKQKHEDMVRFEIMDK 240 Query: 241 FGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWKQMREELVHDT 300 FGYY+TESSEHNAEYHPYFIKR +PELIE IPLDEYPRRCV QI +W+QMRE LV + Sbjct: 241 FGYYVTESSEHNAEYHPYFIKRTHPELIENLNIPLDEYPRRCVAQIEQWRQMRESLVENK 300 Query: 301 TLHHERSHEYASYILEAIETNVPFKIGGNVLNTGLITNLPREACVEVPCLVDASGVTPTY 360 L H R+HEYASY+LEA+ETN P+K+GGNV+NTGLI NLPREACVEVPCLVDASGVTPTY Sbjct: 301 NLQHIRTHEYASYLLEAMETNQPYKLGGNVMNTGLIENLPREACVEVPCLVDASGVTPTY 360 Query: 361 VGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSMDDIVAMCDEL 420 VG LP QCAALNRTNINTQL+TIEAA T K+E +Y AA LDPHT++EL++D+IVA+CD+L Sbjct: 361 VGRLPEQCAALNRTNINTQLMTIEAAITRKREAVYQAAFLDPHTASELTLDEIVALCDDL 420 Query: 421 IAAHGQWLPDF 431 I AHG WLP+F Sbjct: 421 IEAHGNWLPEF 431 >C1PQI8.1 C1PQI8_ALIAC Alpha-galactosidase EC=3.2.1.22 Length = 432 Score = 697 bits (1799), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/431 (75%), Positives = 371/431 (86%) Query: 1 MNKITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGST 60 M+KI FIGAGSTVFAKNVLGDCM T +L+GFE ALFDID ERL+DS RML N+K + Sbjct: 1 MDKIAFIGAGSTVFAKNVLGDCMLTPSLRGFEFALFDIDEERLKDSERMLLNLKEQLRAD 60 Query: 61 CIVKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN 120 ++AY DRK+AL GAKYVINAIQVGGY+PCT+ DFE+PKKYGLRQTI DTVGIGGIFR Sbjct: 61 VHIQAYRDRKQALTGAKYVINAIQVGGYEPCTVIDFEVPKKYGLRQTIGDTVGIGGIFRA 120 Query: 121 LRTIPVMLDFAADIREVCPDALFLNYTNPMAVLTNVMNTYGGVQTVGLCHSVQHCIPGLF 180 LRTIPVML A D++EVCPDA FLNYTNPMAVLTN+M T+GG++TVGLCHSVQ C+P LF Sbjct: 121 LRTIPVMLSIAEDMQEVCPDAWFLNYTNPMAVLTNIMITHGGIKTVGLCHSVQVCVPNLF 180 Query: 181 EALGMDQTGVQAKIAGINHMAWLLEVTKDGEDLYPEIKRRAAEKQKEHHHDMVRYEMMLK 240 +L MD GV+ KIAGINHMAWLLEV KDG DLYPEIKRRA EKQK+ H DMVR+E+M K Sbjct: 181 RSLNMDPEGVEYKIAGINHMAWLLEVKKDGVDLYPEIKRRAREKQKQKHEDMVRFEIMDK 240 Query: 241 FGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWKQMREELVHDT 300 FGYY+TESSEHNAEYHPYFIKR +PELIE IPLDEYPRRCV QI +W+QMR+ LV + Sbjct: 241 FGYYVTESSEHNAEYHPYFIKRTHPELIENLNIPLDEYPRRCVAQIEQWRQMRKSLVENK 300 Query: 301 TLHHERSHEYASYILEAIETNVPFKIGGNVLNTGLITNLPREACVEVPCLVDASGVTPTY 360 L H R+HEYASY+LEA+ETN P+K+GGNV+NTGLI NLPREACVEVPCLVDASGVTPTY Sbjct: 301 NLQHTRTHEYASYLLEAMETNQPYKLGGNVMNTGLIENLPREACVEVPCLVDASGVTPTY 360 Query: 361 VGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSMDDIVAMCDEL 420 VG LP QCAALNRTNINTQL+TIEAA T K+E +Y AA LDPHT++EL++D+IVA+CD+L Sbjct: 361 VGRLPEQCAALNRTNINTQLMTIEAAITRKREAVYQAAFLDPHTASELTLDEIVALCDDL 420 Query: 421 IAAHGQWLPDF 431 I AHG WLP+F Sbjct: 421 IEAHGNWLPEF 431 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,592,503,099 Number of extensions: 107543415 Number of successful extensions: 254903 Number of sequences better than 1.0e-05: 832 Number of HSP's gapped: 252810 Number of HSP's successfully gapped: 873 Length of query: 432 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 293 Effective length of database: 1,751,175,118 Effective search space: 513094309574 Effective search space used: 513094309574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)