BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0012.p (534 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P43263.1 NPRE_BREBE Bacillolysin EC=3.4.24.28 495 e-138 C0Z7R3.1 C0Z7R3_BREBN Bacillolysin EC=3.4.24.28 486 e-135 P29148.1 NPRE_PAEPO Bacillolysin EC=3.4.24.28 476 e-132 C2W3Z5.1 C2W3Z5_BACCE Bacillolysin 427 e-117 C2XPA3.1 C2XPA3_BACCE Bacillolysin 400 e-109 >P43263.1 NPRE_BREBE Bacillolysin EC=3.4.24.28 Length = 527 Score = 495 bits (1274), Expect = e-138, Method: Compositional matrix adjust. Identities = 270/534 (50%), Positives = 338/534 (63%), Gaps = 9/534 (1%) Query: 1 MKKS-LASLLSGIVALGSFASVGIAAESTERSQPAELFTGSTPISITGQSLKTPAGDGSE 59 MKKS LA+ L+ +A+G + A + + + + TP I + P G + Sbjct: 1 MKKSYLATSLTLSIAVGVSGFTSVPAFAKTKIDYHKQW--DTPQYIG--EVWEPEGAKGD 56 Query: 60 EQVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTL 119 + V+ YLE K + N+ F+I + N +T ++HYRL + NGIP+YG QT+ Sbjct: 57 DVVWSYLEKYKDEFRIQG--NVEDHFEIVNEARNKETDTKHYRLQEVYNGIPIYGFQQTV 114 Query: 120 HIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAP 179 HID GNVTS LG + D+ + L + +++ +A+ A+ D + G + P Sbjct: 115 HIDADGNVTSFLGQFIPDLDSN--KQLKKKPKLNEQKAVKQAIKDVEGEVGEKPDFIQDP 172 Query: 180 EADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDLTGTGTGVN 239 EA LY ++ E LAY E+N L+PEP R YFI A G V+ KYN+L+ +T TG GV Sbjct: 173 EAKLYIYVHEDESYLAYAVELNFLDPEPGRWMYFIDAHSGDVINKYNMLDHVTATGKGVL 232 Query: 240 GDTKTFETRLSGSTYQLYDNTRGKGIVTYTAKNRXXXXXXXXXXXXNVWSDRAGVDAHTY 299 GDTK FET GSTY L D TRGKGI TYTA NR N W+D A VDAH + Sbjct: 233 GDTKQFETTKQGSTYMLKDTTRGKGIETYTANNRTSLPGTLMTDSDNYWTDGAAVDAHAH 292 Query: 300 AERTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSYNNAFWNGSQIVFGDGDGTTFTLLSGD 359 A++TYDY+ RNS D G IRS+VHY T YNNAFWNGSQ+V+GDGDGTTF LSG Sbjct: 293 AQKTYDYFRNVHNRNSYDGNGAVIRSTVHYSTRYNNAFWNGSQMVYGDGDGTTFLPLSGG 352 Query: 360 LDVVGHELTHGVTENTANLEYYGEPGALNESFSDIIGNSIEGENWLVGDKVYTPGIPGDA 419 LDVV HELTH VTE TA L Y E GALNES SDI G ++ ++WL+G+ +YTPG GDA Sbjct: 353 LDVVAHELTHAVTERTAGLVYQNESGALNESMSDIFGAMVDNDDWLMGEDIYTPGRSGDA 412 Query: 420 LRSLANPTLYGQPDKYSDRYTGSSDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIGRE 479 LRSL +P YG PD YS RYTGS DNGGVHTNSGINNKA YL+A+GGT GV V GIGR Sbjct: 413 LRSLQDPAAYGDPDHYSKRYTGSQDNGGVHTNSGINNKAAYLLAEGGTHYGVRVNGIGRT 472 Query: 480 DAVQIYYNALVYYLTTSSDFSAARAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 D +IYY+AL +YLT S+FSA R A + SAT+LFGA S QV AV AY+AVGV Sbjct: 473 DTAKIYYHALTHYLTPYSNFSAMRRAAVLSATDLFGANSRQVQAVNAAYDAVGV 526 >C0Z7R3.1 C0Z7R3_BREBN Bacillolysin EC=3.4.24.28 Length = 528 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 265/536 (49%), Positives = 344/536 (64%), Gaps = 12/536 (2%) Query: 1 MKKSL-ASLLSGIVALGSFASVGIAAESTERSQPAELFTGSTPISITGQSLKTPAGDGSE 59 MKKS+ A LS + +F + A S AE TP I G+ + DG E Sbjct: 1 MKKSVFAVTLSFALGTTTFLPLATQAASESIVYSAEW---DTPEFI-GEEFEAEELDG-E 55 Query: 60 EQVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTL 119 E+V+ +LE + + + +++ F++ ++ T+ +T HYR+ + GIPVYG QT+ Sbjct: 56 EKVWGFLEQYQDSFRIDG--DVKDHFKVLDEVTDKETDMTHYRVQEMYEGIPVYGYQQTI 113 Query: 120 HIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYG-PLGEAQIA 178 H+++ GNVT+ LG D+ L + + +A+ A+ D + + + ++ Sbjct: 114 HVNEDGNVTAFLGNYAPDLSNN--DKLTEKPKLKADKAVKEAIKDLEDEINEKIEKFEVK 171 Query: 179 PEADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDLTGTGTGV 238 P+ DLY +I E LAY+ E+N L+P P R +YFI A G V+ KYN+L ++TGTGTGV Sbjct: 172 PKGDLYVYIHEDESYLAYEVELNFLDPLPGRWKYFIDANSGEVINKYNMLHEITGTGTGV 231 Query: 239 NGDTKTFE-TRLSGSTYQLYDNTRGKGIVTYTAKNRXXXXXXXXXXXXNVWSDRAGVDAH 297 GDTK+FE T++S + Y D TRGKGI TYTA+NR N W+D A VDAH Sbjct: 232 LGDTKSFEVTKVSNNNYTAKDTTRGKGIETYTARNRTTLPGTLGSDTDNNWTDGALVDAH 291 Query: 298 TYAERTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSYNNAFWNGSQIVFGDGDGTTFTLLS 357 YA++TYDYY RNS DN G ++ S+VHY YNNAFWNG+Q+V+GDGDG+ F LS Sbjct: 292 AYAQKTYDYYKNAHNRNSFDNNGAKLISTVHYSNRYNNAFWNGTQMVYGDGDGSVFRPLS 351 Query: 358 GDLDVVGHELTHGVTENTANLEYYGEPGALNESFSDIIGNSIEGENWLVGDKVYTPGIPG 417 LDVV HELTH VTE TANL Y E GALNES SDI G I+ ++WL+G+ VYTPG PG Sbjct: 352 AGLDVVAHELTHAVTERTANLIYQNESGALNESISDIFGAMIDDDDWLMGEDVYTPGTPG 411 Query: 418 DALRSLANPTLYGQPDKYSDRYTGSSDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIG 477 DALRSL +PT+ G PD YSDRYTG +DNGGVHTNSGINNKA YL+A+GGT GVTV GIG Sbjct: 412 DALRSLEDPTVAGDPDHYSDRYTGPNDNGGVHTNSGINNKAAYLLAEGGTHTGVTVTGIG 471 Query: 478 REDAVQIYYNALVYYLTTSSDFSAARAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 R D +IYY AL YL +S+FSA R A IQSAT LFGA S QV +V AY+AVGV Sbjct: 472 RNDTAKIYYYALTNYLNPNSNFSAMRQAAIQSATALFGANSQQVKSVKAAYDAVGV 527 >P29148.1 NPRE_PAEPO Bacillolysin EC=3.4.24.28 Length = 590 Score = 476 bits (1225), Expect = e-132, Method: Compositional matrix adjust. Identities = 293/600 (48%), Positives = 374/600 (62%), Gaps = 78/600 (13%) Query: 1 MKKSLASLLSGIVALGSFASVGIAAESTERSQPAELFTGSTPISITGQSLKTPAGDGSEE 60 MKK SLL G + LGS AS G +AES+ S PA+L TP T Q K P+ ++ Sbjct: 1 MKKVWFSLLGGAMLLGSVAS-GASAESS-VSGPAQL----TPTFHTEQ-WKAPSSVSGDD 53 Query: 61 QVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTLH 120 V+ YL K +L ++R +F+I ++ T+ K+G HYRL QY+NGIPVYGA+QT+H Sbjct: 54 IVWSYLNRQKKSLLGVDSSSVREQFRIVDR-TSDKSGVSHYRLKQYVNGIPVYGAEQTIH 112 Query: 121 IDKSGNVTSLLGTVV-EDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYG--PLGEAQI 177 + KSG VTS LG V+ ED + Q IS +EA+ A +A + P + I Sbjct: 113 VGKSGEVTSYLGAVINEDQQEEATQGTT--PKISASEAVYTAYKEAAARIEALPTSDDTI 170 Query: 178 APEA--------DLYYFIVGGEPKLAY-KTEVNV-------------------------- 202 + +A D Y + L+ K E+++ Sbjct: 171 SKDAEEPSSVSKDTYAEAANNDKTLSVDKDELSLDKASVLKDSKIEAVEAEKSSIAKIAN 230 Query: 203 LEPE---PLRIRYFISAEDGTVLF--KYNILE---------------------DLT--GT 234 L+PE + Y+ +D +++ + N+LE D+ T Sbjct: 231 LQPEVDPKAELYYYPKGDDLLLVYVTEVNVLEPAPLRTRYIIDANDGSIVFQYDIINEAT 290 Query: 235 GTGVNGDTKTFETRLSGSTYQLYDNTRGKGIVTYTAKNRXXXXXXXXXXXXNVWSDRAGV 294 G GV GD+K+F T SGS+YQL D TRG GIVTYTA NR NVW+D AGV Sbjct: 291 GKGVLGDSKSFTTTASGSSYQLKDTTRGNGIVTYTASNRQSIPGTLLTDADNVWNDPAGV 350 Query: 295 DAHTYAERTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSYNNAFWNGSQIVF--GDGDGTT 352 DAH YA +TYDYY FGRNS+D +GLQ+RS+VHY + YNNAFWNGSQ+ + GDGDG+T Sbjct: 351 DAHAYAAKTYDYYKSKFGRNSIDGRGLQLRSTVHYGSRYNNAFWNGSQMTYGDGDGDGST 410 Query: 353 FTLLSGDLDVVGHELTHGVTENTANLEYYGEPGALNESFSDIIGNSIEGENWLVGDKVYT 412 F SGD DVVGHELTHGVTE T+NLEYYGE GALNE+FSD+IGN I+ +NWLVGD +YT Sbjct: 411 FIAFSGDPDVVGHELTHGVTEYTSNLEYYGESGALNEAFSDVIGNDIQRKNWLVGDDIYT 470 Query: 413 PGIPGDALRSLANPTLYGQPDKYSDRYTGSSDNGGVHTNSGINNKAFYLVAQGGTFNGVT 472 P I GDALRS++NPTLY QP YS+ Y GSSDNGGVHTNSGI NKA+YL+AQGGTF+GVT Sbjct: 471 PNICGDALRSMSNPTLYDQPHHYSNLYKGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVT 530 Query: 473 VAGIGREDAVQIYYNALVYYLTTSSDFSAARAAVIQSATELFGAGSAQVTAVTQAYNAVG 532 V GIGR+ AVQIYY+A YLT+SSDFS ARAAVIQ+A +L+GA SA+ TA ++++AVG Sbjct: 531 VNGIGRDAAVQIYYSAFTNYLTSSSDFSNARAAVIQAAKDLYGANSAEATAAAKSFDAVG 590 >C2W3Z5.1 C2W3Z5_BACCE Bacillolysin Length = 566 Score = 427 bits (1098), Expect = e-117, Method: Compositional matrix adjust. Identities = 258/571 (45%), Positives = 336/571 (58%), Gaps = 47/571 (8%) Query: 2 KKSLASLLSGIVALGSFASVGIAAESTERSQPAELFTG--STPISITGQSLKTPAGDGSE 59 KKSLA +L+ +A+ +F G A ++ A+ + +P I+G L G +E Sbjct: 3 KKSLALVLAAGMAVTTFGGTGSAFADSKNVLSAKKYNEVIQSPEFISGD-LTGATGKKAE 61 Query: 60 EQVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTL 119 V+ YL + K + L K + F++K+ + ++ T S R+ Q G PV+G+ Q Sbjct: 62 SVVFDYLNAAKVDYKLGKK-SAEDSFKVKQVKKDTVTDSTVVRMQQMYEGTPVWGSTQLA 120 Query: 120 HIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAP 179 H+ K G++ L GTVV D+ + L I +AI A Q G + ++ P Sbjct: 121 HVSKDGSLKVLSGTVVPDL--DKKEKLKNKNKIEGKKAIGIA----QQDLGFTPKYEVDP 174 Query: 180 EADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDLT------- 232 ADLY + G + AY ++N LEP+P YFI A+ G VL KYN ++ +T Sbjct: 175 SADLYVYQNGEDVTYAYVVKLNFLEPQPGNYYYFIEADSGKVLNKYNTIDHVTDEDKAPL 234 Query: 233 ----------------------GTGTGVNGDTKTFETRLSGSTYQLYDNTRGKGIVTYTA 270 GTG GV GDTK+ T LSGSTY L DNTRG I TY A Sbjct: 235 NKEVVKQEAKTAGKPVTGTNKVGTGKGVLGDTKSLNTTLSGSTYYLQDNTRGAQIFTYDA 294 Query: 271 KNRXXXXXXXXXXXXNVWS---DRAGVDAHTYAERTYDYYLQHFGRNSLDNKGLQIRSSV 327 KNR NV++ D A VDAH YA TYDYY F RNS+++ G ++S+V Sbjct: 295 KNRTTLPGTLWADTDNVFNASYDAAAVDAHYYAGVTYDYYKNTFNRNSINDAGAPLKSTV 354 Query: 328 HYYTSYNNAFWNGSQIVFGDGDGTTFTLLSGDLDVVGHELTHGVTENTANLEYYGEPGAL 387 HY SYNNAFWNGSQ+V+GDGDG TF LSG LDV+GHELTH VTE ++NL Y E GAL Sbjct: 355 HYGRSYNNAFWNGSQMVYGDGDGATFAPLSGALDVIGHELTHAVTEYSSNLIYQNESGAL 414 Query: 388 NESFSDIIGNSIE-----GENWLVGDKVYTPGIPGDALRSLANPTLYGQPDKYSDRYTGS 442 NE+FSD+ G +E +W VG+ VYTPG GDALRS+++PT YG PD YS RYTG+ Sbjct: 415 NEAFSDVFGTLVEYYDNRNPDWEVGEDVYTPGKSGDALRSMSDPTKYGDPDHYSKRYTGT 474 Query: 443 SDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIGREDAVQIYYNALVYYLTTSSDFSAA 502 SDNGGVHTNSGI NKA YL+A GGT GVTV GIG++ IYY A Y T S+ FS A Sbjct: 475 SDNGGVHTNSGIINKAAYLLANGGTHYGVTVNGIGKDKVGAIYYRANTLYFTESTTFSQA 534 Query: 503 RAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 RA ++Q+A +L+GA SA+VTAV Q+Y+AVGV Sbjct: 535 RAGLVQAAADLYGASSAEVTAVKQSYDAVGV 565 >C2XPA3.1 C2XPA3_BACCE Bacillolysin Length = 566 Score = 400 bits (1029), Expect = e-109, Method: Compositional matrix adjust. Identities = 248/571 (43%), Positives = 332/571 (58%), Gaps = 47/571 (8%) Query: 2 KKSLASLLSGIVALGSFASVGIAAESTERSQPAELF--TGSTPISITGQSLKTPAGDGSE 59 +KSLA +L+ +A+ +F G A ++ + + T +P I+G L G +E Sbjct: 3 RKSLALVLATGMAVTAFGGTGSAFADSKNVLSTKKYNETVQSPEFISGD-LTGATGKKAE 61 Query: 60 EQVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTL 119 V+ YL + KG+ L K + + F++K+ + +S T S R+ Q G+PV+G+ Q Sbjct: 62 SVVFDYLNAAKGDYKLGEK-SAQDSFKVKQVKKDSVTDSTVVRMQQVYEGVPVWGSTQVA 120 Query: 120 HIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAP 179 H+ K G++ L GTV D+ + L I +AI A Q G + ++ P Sbjct: 121 HVSKDGSLKVLSGTVAPDL--DKKEKLKNKNKIEGAKAIEIA----QQDLGVTPKYEVDP 174 Query: 180 EADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDLT------- 232 +ADLY + G E AY +N L+P P YFI A+ G VL KYN ++ +T Sbjct: 175 KADLYVYQNGEETTYAYVVNLNFLDPSPGNYYYFIEADSGKVLNKYNTIDHVTNDDKSPV 234 Query: 233 ----------------------GTGTGVNGDTKTFETRLSGSTYQLYDNTRGKGIVTYTA 270 GTG GV GDTK+ T LSGS+Y L DNTRG I TY A Sbjct: 235 KQDAPKQDAKAVVKPVTGTNKVGTGIGVLGDTKSLNTTLSGSSYYLQDNTRGATIFTYDA 294 Query: 271 KNRXXXXXXXXXXXXNVWSDRAGVDA---HTYAERTYDYYLQHFGRNSLDNKGLQIRSSV 327 KNR NV++ A H YA +TYDYY F RNS+++ G ++S+V Sbjct: 295 KNRSTLPGTLWADADNVFNAAYDAAAVDAHYYAGKTYDYYKDTFNRNSINDAGAPLKSTV 354 Query: 328 HYYTSYNNAFWNGSQIVFGDGDGTTFTLLSGDLDVVGHELTHGVTENTANLEYYGEPGAL 387 HY + YNNAFWNGSQ+V+GDGDG TFT LSG +DV+GHELTH VTEN++NL Y E GAL Sbjct: 355 HYGSKYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTENSSNLIYQNESGAL 414 Query: 388 NESFSDIIGNSIE-----GENWLVGDKVYTPGIPGDALRSLANPTLYGQPDKYSDRYTGS 442 NE+ SDI G +E +W +G+ +YTPG GDALRS+++P YG PD YS RYTGS Sbjct: 415 NEAISDIFGTLVEFYDNRNPDWEIGEDIYTPGKAGDALRSMSDPAKYGDPDHYSKRYTGS 474 Query: 443 SDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIGREDAVQIYYNALVYYLTTSSDFSAA 502 SDNGGVHTNSGI NK YL+A GGT +GVTV GIG++ IYY A Y T S+ FS A Sbjct: 475 SDNGGVHTNSGIINKQAYLLANGGTHSGVTVTGIGKDKLGAIYYRANTQYFTQSTTFSQA 534 Query: 503 RAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 RA +Q+A +L+GA SA+V AV Q+++AVGV Sbjct: 535 RAGAVQAAADLYGASSAEVAAVKQSFSAVGV 565 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,275,295,286 Number of extensions: 148524104 Number of successful extensions: 270459 Number of sequences better than 1.0e-05: 1030 Number of HSP's gapped: 266725 Number of HSP's successfully gapped: 1033 Length of query: 534 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 393 Effective length of database: 1,732,333,088 Effective search space: 680806903584 Effective search space used: 680806903584 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 134 (56.2 bits)