BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0021.p (344 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKS7.1 B3KKS7_9BACI Histidine kinase dimerisation and phosphoa... 482 e-134 B4AG94.1 B4AG94_BACPU Sensor protein LiaS EC=2.7.13.3 266 3e-69 A8FHA1.1 A8FHA1_BACP2 Sensor histidine kinase 265 4e-69 O32198.1 LIAS_BACSU Sensor histidine kinase liaS EC=2.7.13.3 262 3e-68 Q65F44.1 Q65F44_BACLD YvqE 262 4e-68 >B3KKS7.1 B3KKS7_9BACI Histidine kinase dimerisation and phosphoacceptor region Length = 357 Score = 482 bits (1240), Expect = e-134, Method: Compositional matrix adjust. Identities = 241/342 (70%), Positives = 294/342 (85%) Query: 3 KKSSNMVTRSMGEGALFSLVMLLVILYIMYTYGYLQPFEDWKIGVRTAVTLILLPVVFGI 62 +K +N++TRSM EG L LV++LV+LY++YTYGYLQPFE WK + +TL+LLP+ G Sbjct: 4 EKKANIITRSMREGTLLGLVVMLVVLYLLYTYGYLQPFETWKNRIEAGITLVLLPIGLGA 63 Query: 63 VFGFYQGFRTKRKLERLRATLVAWEKGNLTPAMPDLGNDELGRLSGQLSRISGKWENQVN 122 ++GF+QG+R KR+++ +R TLV WEKG+LT MPD+G DE+GRL QL +IS +WE+QV+ Sbjct: 64 IYGFFQGYRVKRRVDLVRETLVNWEKGSLTDVMPDIGEDEVGRLGEQLVKISKRWEDQVS 123 Query: 123 TLQRLSTNNAKLAEQARVSAIMEERQRLARELHDAVSQQLFAISMTATAVGRTLEKDFDK 182 +LQRLSTNNA+LAEQARV+AI+EERQRLARELHDAVSQQLFAISMTATAVGRTL+KDFDK Sbjct: 124 SLQRLSTNNAQLAEQARVTAIVEERQRLARELHDAVSQQLFAISMTATAVGRTLDKDFDK 183 Query: 183 AQRQIALIEEMSAVAQSEMRALLLHLRPVYLEGKGLREGLQXXXXXXXXXXPIDIVFEMD 242 AQRQ+ALIEEMSAVAQSEMRALLLHLRPVYLEGK L +GL+ P++I FEMD Sbjct: 184 AQRQVALIEEMSAVAQSEMRALLLHLRPVYLEGKALEQGLRDLITELRIKVPMEITFEMD 243 Query: 243 PDVQLMKGVENHLFRIVQEAISNTLRHAKAEKMEIRLHRRGDTVRLTLRDDGVGFEMDES 302 D+ L+KG+ENHLFRI+QEA+SNTLRH+KAEKM+IR+HRR +TVR+ LRDDGVGFE+D+ Sbjct: 244 DDIHLLKGIENHLFRIIQEAMSNTLRHSKAEKMDIRIHRRQNTVRVMLRDDGVGFELDDK 303 Query: 303 KQTSYGLSNMQERINETGGSIQFITAPGKGMRIEITVPLVNE 344 KQTSYGLS MQER+ E GGS+QFITAPGKG RIEI VPL+NE Sbjct: 304 KQTSYGLSTMQERVTEIGGSLQFITAPGKGTRIEIIVPLINE 345 >B4AG94.1 B4AG94_BACPU Sensor protein LiaS EC=2.7.13.3 Length = 354 Score = 266 bits (679), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 141/333 (42%), Positives = 213/333 (63%), Gaps = 5/333 (1%) Query: 16 GALFSLVMLLVILYIMYTYGYLQPF----EDWKIGVRTAVTLILLPVVFGIVFGFYQGFR 71 G SL ++++ + M+ Y L P W G+ L+++ + G G++ Sbjct: 16 GVGISLGVVIMTMLPMFFYYQLDPMILLSTRW-FGIPFFCILLIISLAIGFSASHVFGYQ 74 Query: 72 TKRKLERLRATLVAWEKGNLTPAMPDLGNDELGRLSGQLSRISGKWENQVNTLQRLSTNN 131 K++L++L +++ +E GN +P LG+DE+G + QL+ ++ + E QV +LQ+LS Sbjct: 75 HKKRLDQLVESILKFENGNFAYRLPSLGDDEIGLAADQLNEMAKRVEGQVASLQKLSNER 134 Query: 132 AKLAEQARVSAIMEERQRLARELHDAVSQQLFAISMTATAVGRTLEKDFDKAQRQIALIE 191 A+ Q + S + EERQRLARELHDAVSQQLFAISM +A+ +++ +K +Q+ L+E Sbjct: 135 AEWQVQMKKSVVSEERQRLARELHDAVSQQLFAISMMTSAILEVMKEKDEKILKQMRLVE 194 Query: 192 EMSAVAQSEMRALLLHLRPVYLEGKGLREGLQXXXXXXXXXXPIDIVFEMDPDVQLMKGV 251 M+ AQ+EMRALLLHLRP+ LEGK L++GL PIDI +E++ DV L KGV Sbjct: 195 RMAGDAQNEMRALLLHLRPITLEGKDLKKGLIELLNEFQAKQPIDIDWEIEDDVPLSKGV 254 Query: 252 ENHLFRIVQEAISNTLRHAKAEKMEIRLHRRGDTVRLTLRDDGVGFEMDESKQTSYGLSN 311 E+HLFRIVQEA+SN RHAKA K+ +RL R V+L + D+G+GF+ D K SYGL + Sbjct: 255 EDHLFRIVQEALSNVFRHAKATKVTVRLLIRNRQVQLKIIDNGIGFQTDHVKTASYGLES 314 Query: 312 MQERINETGGSIQFITAPGKGMRIEITVPLVNE 344 ++ER +E GG + ++ GKG +I++ VP+ +E Sbjct: 315 IRERTSEVGGVAEIMSFEGKGTQIDVKVPIFDE 347 >A8FHA1.1 A8FHA1_BACP2 Sensor histidine kinase Length = 354 Score = 265 bits (677), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 141/333 (42%), Positives = 213/333 (63%), Gaps = 5/333 (1%) Query: 16 GALFSLVMLLVILYIMYTYGYLQPF----EDWKIGVRTAVTLILLPVVFGIVFGFYQGFR 71 G SL ++++ + M+ Y L P W G+ L+++ + G G++ Sbjct: 16 GVGISLGVVIMTMLPMFFYYQLDPMILLSTRW-FGIPFFCILLIISLAIGFSASHVFGYQ 74 Query: 72 TKRKLERLRATLVAWEKGNLTPAMPDLGNDELGRLSGQLSRISGKWENQVNTLQRLSTNN 131 K++L++L +++ +E GN +P LG+DE+G + QL+ ++ + E QV +LQ+LS Sbjct: 75 HKKRLDQLVESILKFENGNFAYRLPSLGDDEIGLAADQLNEMAKRVEGQVASLQKLSNER 134 Query: 132 AKLAEQARVSAIMEERQRLARELHDAVSQQLFAISMTATAVGRTLEKDFDKAQRQIALIE 191 A+ Q + S + EERQRLARELHDAVSQQLFAISM +A+ +++ +K +Q+ L+E Sbjct: 135 AEWQVQMKKSVVSEERQRLARELHDAVSQQLFAISMMTSAILEGMKEKDEKILKQMRLVE 194 Query: 192 EMSAVAQSEMRALLLHLRPVYLEGKGLREGLQXXXXXXXXXXPIDIVFEMDPDVQLMKGV 251 M+ AQ+EMRALLLHLRP+ LEGK L++GL PIDI +E++ DV L KGV Sbjct: 195 RMAGDAQNEMRALLLHLRPITLEGKDLKKGLIELLNEFQAKQPIDIDWEIEDDVPLSKGV 254 Query: 252 ENHLFRIVQEAISNTLRHAKAEKMEIRLHRRGDTVRLTLRDDGVGFEMDESKQTSYGLSN 311 E+HLFRIVQEA+SN RHAKA K+ +RL R V+L + D+G+GF+ D K SYGL + Sbjct: 255 EDHLFRIVQEALSNVFRHAKATKVTVRLLIRNRQVQLKIIDNGIGFQTDHVKTASYGLES 314 Query: 312 MQERINETGGSIQFITAPGKGMRIEITVPLVNE 344 ++ER +E GG + ++ GKG +I++ VP+ +E Sbjct: 315 IRERTSEVGGVAEIMSFEGKGTQIDVKVPIFDE 347 >O32198.1 LIAS_BACSU Sensor histidine kinase liaS EC=2.7.13.3 Length = 360 Score = 262 bits (670), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 145/330 (43%), Positives = 210/330 (63%), Gaps = 6/330 (1%) Query: 19 FSLVMLLVILYIMYTYGYLQPF----EDWKIGVRTAVTLILLPVVFGIVFGFYQGFRTKR 74 SL++ + ++ M Y L P W G+ + L+L+ V G G+ G R K Sbjct: 20 ISLLLFVCLISFMMFYYRLDPLVLLSSSW-FGIPFILILLLISVTVGFASGYMYGNRLKT 78 Query: 75 KLERLRATLVAWEKGNLTPAMPDLGNDELGRLSGQLSRISGKWENQVNTLQRLSTNNAKL 134 +++ L +++ +E GN +P LG+DE+G + QL+ ++ + E QV +LQ+LS A+ Sbjct: 79 RIDTLIESILTFENGNFAYRIPPLGDDEIGLAADQLNEMAKRVELQVASLQKLSNERAEW 138 Query: 135 AEQARVSAIMEERQRLARELHDAVSQQLFAISMTATAVGRTLEKDFDKAQRQIALIEEMS 194 Q + S I EERQRLAR+LHDAVSQQLFAISM +AV ++ DK ++I ++E M+ Sbjct: 139 QAQMKKSVISEERQRLARDLHDAVSQQLFAISMMTSAVLEHVKDADDKTVKRIRMVEHMA 198 Query: 195 AVAQSEMRALLLHLRPVYLEGKGLREGLQXXXXXXXXXXPIDIVFEMDPDVQLMKGVENH 254 AQ+EMRALLLHLRPV LEGKGL+EGL PIDI +++ D + KGVE+H Sbjct: 199 GEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPIDIEWDIQ-DTAISKGVEDH 257 Query: 255 LFRIVQEAISNTLRHAKAEKMEIRLHRRGDTVRLTLRDDGVGFEMDESKQTSYGLSNMQE 314 LFRIVQEA+SN RH+KA K+ + L + +RL + D+G GF+MD+ K +SYGL++M+E Sbjct: 258 LFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGFKMDQVKASSYGLNSMKE 317 Query: 315 RINETGGSIQFITAPGKGMRIEITVPLVNE 344 R +E GG + I+ GKG +IE+ VP+ E Sbjct: 318 RASEIGGVAEVISVEGKGTQIEVKVPIFPE 347 >Q65F44.1 Q65F44_BACLD YvqE Length = 354 Score = 262 bits (669), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 144/330 (43%), Positives = 210/330 (63%), Gaps = 6/330 (1%) Query: 20 SLVMLLVILYIMYTYGYLQP----FEDWKIGVRTAVTLILLPVVFGIVFGFYQGFRTKRK 75 S + L +L M Y L P W G+ L L+ G G+ G + K++ Sbjct: 21 SFTIFLAVLGFMLFYYQLDPRVLIVSRW-FGIPFIALLALISTAVGFGSGYMFGNQLKKR 79 Query: 76 LERLRATLVAWEKGNLTPAMPDLGNDELGRLSGQLSRISGKWENQVNTLQRLSTNNAKLA 135 LE+L +++ +E GN +P LG+DE+G + QL+ ++ + E QV +LQ+LS A+ Sbjct: 80 LEKLIESILKYENGNFAYRIPSLGDDEIGLAADQLNEMAERIERQVASLQKLSNERAEWQ 139 Query: 136 EQARVSAIMEERQRLARELHDAVSQQLFAISMTATAVGRTLEKDFD-KAQRQIALIEEMS 194 +Q + S I EERQRLARELHDAVSQQLFAISM +AV ++ D K +++ ++E+M+ Sbjct: 140 DQMKKSVISEERQRLARELHDAVSQQLFAISMMTSAVLEGIDAAADEKLAQRLKMVEKMA 199 Query: 195 AVAQSEMRALLLHLRPVYLEGKGLREGLQXXXXXXXXXXPIDIVFEMDPDVQLMKGVENH 254 A AQ+EMRALLLHLRPV LEGKGL+EGL IDI +E++ +L KGVE+H Sbjct: 200 ADAQNEMRALLLHLRPVTLEGKGLQEGLADLLKEFKAKQSIDIDWEIEDAGKLPKGVEDH 259 Query: 255 LFRIVQEAISNTLRHAKAEKMEIRLHRRGDTVRLTLRDDGVGFEMDESKQTSYGLSNMQE 314 LFRIVQEA+SN RH+KA K+ +RL R ++L + D+G GF+MD K +SYGL++++E Sbjct: 260 LFRIVQEALSNVFRHSKATKVTVRLLLRNKQLQLKVIDNGEGFKMDAVKTSSYGLNSIKE 319 Query: 315 RINETGGSIQFITAPGKGMRIEITVPLVNE 344 R +E GG + I+ GKG ++++ VP+ + Sbjct: 320 RASEVGGVAEIISFTGKGTQVDVKVPIFEQ 349 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,781,838,798 Number of extensions: 67710954 Number of successful extensions: 274738 Number of sequences better than 1.0e-05: 3586 Number of HSP's gapped: 269738 Number of HSP's successfully gapped: 3587 Length of query: 344 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 208 Effective length of database: 1,779,438,163 Effective search space: 370123137904 Effective search space used: 370123137904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)