BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0016.p (225 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKT1.1 B3KKT1_9BACI PspA/IM30 family protein 313 8e-84 B1DF42.1 B1DF42_9BACL Phage shock protein A, PspA 223 7e-57 C0Z624.1 C0Z624_BREBN Putative uncharacterized protein 88 6e-16 B0TET3.1 B0TET3_HELMI Phage shock protein a 88 6e-16 Q8CV17.1 Q8CV17_OCEIH Phage shock protein A (Membrane protein) 86 2e-15 >B3KKT1.1 B3KKT1_9BACI PspA/IM30 family protein Length = 225 Score = 313 bits (802), Expect = 8e-84, Method: Compositional matrix adjust. Identities = 155/225 (68%), Positives = 181/225 (80%) Query: 1 MSVFRSMRDITVANLNERLEQSQDPVKLIDQFLHSTREEITEAERLYQQYAAHTKQLQQQ 60 MSVFR +RDITVA LNE+LEQSQDPV+LIDQFLHSTR++I EAE+L QQ H++QL+QQ Sbjct: 1 MSVFRRIRDITVATLNEKLEQSQDPVRLIDQFLHSTRQDIAEAEKLQQQCQVHSRQLKQQ 60 Query: 61 VNQATAMRNKREEQALLALKAGEDHLAKLALQEKIIHEEKXXXXXXXXXXXXXXXXXXXX 120 V+QA MR KREEQALLALKAGE+HLAKLALQEK+I+EEK Sbjct: 61 VDQAETMRFKREEQALLALKAGEEHLAKLALQEKLIYEEKIEQYQGLLSSSLESLKEIEE 120 Query: 121 XXXXXKMEYQTVYSKRQYYAARMESLRLQQRMNQRATAYGNGSDVPKMFGRLEDRMSDWE 180 +MEYQTVYSKRQYY AR+E+LRLQQ+MN+R +YG G DVPKMF RLEDR+SDWE Sbjct: 121 QLGELRMEYQTVYSKRQYYVARVETLRLQQKMNERTGSYGGGHDVPKMFNRLEDRISDWE 180 Query: 181 LEAKSLRDLRRMGQEYAIQAGETVATVLEREMARLKEKLNNDGKE 225 LEA+SLRDLRRMGQEY QAGE+V+TVLE+E+ARLK+KLNN GKE Sbjct: 181 LEARSLRDLRRMGQEYMDQAGESVSTVLEKELARLKQKLNNSGKE 225 >B1DF42.1 B1DF42_9BACL Phage shock protein A, PspA Length = 228 Score = 223 bits (569), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 121/225 (53%), Positives = 152/225 (67%), Gaps = 2/225 (0%) Query: 1 MSVFRSMRDITVANLNERLEQSQDPVKLIDQFLHSTREEITEAERLYQQYAAHTKQLQQQ 60 MS+ R +RDITVA+LNERLE+++DPV+LIDQFL STREEI +AERL+QQY H+ L+ Q Sbjct: 1 MSIMRRVRDITVASLNERLEKAEDPVRLIDQFLWSTREEIIQAERLHQQYLGHSNHLKAQ 60 Query: 61 VNQATAMRNKREEQALLALKAGEDHLAKLALQEKIIHEEKXXXXXXXXXXXXXXXXXXXX 120 QA + KRE+QAL ALKAGE+ LA++AL EK EE+ Sbjct: 61 WLQAEQQKEKREQQALTALKAGEEQLARIALHEKTTSEERSNQYRELYEQSRQNTMDLED 120 Query: 121 XXXXXKMEYQTVYSKRQYYAARMESLRLQQRMNQRATAYGNGSDV--PKMFGRLEDRMSD 178 + EYQ VY KR+YYAARMESLRLQQRMN R T G + MF RLEDR+ D Sbjct: 121 QLREMRSEYQLVYDKREYYAARMESLRLQQRMNARYTEGGQAGGIQPGSMFRRLEDRIGD 180 Query: 179 WELEAKSLRDLRRMGQEYAIQAGETVATVLEREMARLKEKLNNDG 223 E+EAKSL DLRRMG+E+ +AG V +V+ERE+ +LK+KL +G Sbjct: 181 MEMEAKSLHDLRRMGKEFISEAGNQVQSVIERELQQLKKKLEKEG 225 >C0Z624.1 C0Z624_BREBN Putative uncharacterized protein Length = 231 Score = 87.8 bits (216), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 63/220 (28%), Positives = 111/220 (50%), Gaps = 1/220 (0%) Query: 1 MSVFRSMRDITVANLNERLEQSQDPVKLIDQFLHSTREEITEAERLYQQYAAHTKQLQQQ 60 MS+F+ +RD+TVA++N+ L+ +DPV +++Q++ EI + E + A K+ +QQ Sbjct: 1 MSIFKRLRDLTVASVNDALDSMEDPVVMLNQYMRDMEVEIGQVEVAVARQVALEKKFRQQ 60 Query: 61 VNQATAMRNKREEQALLALKAGEDHLAKLALQEKIIHEEKXXXXXXXXXXXXXXXXXXXX 120 +++A A+ KR+ Q LAL GED LA+ AL +K +E + Sbjct: 61 LDEANALIEKRDRQVKLALTEGEDELARRALADKKQYEGRAAEYETLYLSASESATQMRE 120 Query: 121 XXXXXKMEYQTVYSKRQYYAARMESLRLQQRMNQRATAYGNGSDVPKMFGRLEDRMSDWE 180 K E+ + +K+ AR + R Q+++NQ GN S + F R+E+++ E Sbjct: 121 KLAELKEEFYKMRAKKFTLMARAQVARTQKQVNQAVVGIGNQS-AGRGFARMEEKVMRME 179 Query: 181 LEAKSLRDLRRMGQEYAIQAGETVATVLEREMARLKEKLN 220 EA+ R+ + + E L+ E+A +K +N Sbjct: 180 AEAQMSGQWRQTNRAWENALSELEKDDLDAELASIKASIN 219 >B0TET3.1 B0TET3_HELMI Phage shock protein a Length = 222 Score = 87.8 bits (216), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 60/222 (27%), Positives = 117/222 (52%), Gaps = 5/222 (2%) Query: 1 MSVFRSMRDITVANLNERLEQSQDPVKLIDQFLHSTREEITEAERLYQQYAAHTKQLQQQ 60 MS+F+ ++D+T++N+N L++++DPVK++DQ+L E+I +A + A TK+ + Q Sbjct: 1 MSLFKRLKDLTLSNINALLDKAEDPVKMLDQYLRDMEEDIRDASDAIAKQIAVTKKFEAQ 60 Query: 61 VNQATAMRNKREEQALLALKAGEDHLAKLALQEKIIHEEKXXXXXXXXXXXXXXXXXXXX 120 +A AM KR+E A+ A+ AG D LA+ AL++K HE + Sbjct: 61 YREAEAMVQKRQEDAVKAINAGRDDLARRALEDKKNHETRMNEYKAQFETHSATSEKLKR 120 Query: 121 XXXXXKMEYQTVYSKRQYYAARMESLRLQQRMNQRATAYGNGSDVPKMFGRLEDRMSDWE 180 + E+ + ++R AR + + ++++ + N S F R+E++++ E Sbjct: 121 QLADMRNEFDKLKARRDTLVARANAAKATKQVHDLLGGFDNRS-ARHGFDRMEEKVASLE 179 Query: 181 LEAKSLRDLRRMGQEYAIQ---AGETVATVLEREMARLKEKL 219 +A++ ++L R G ++ A + ++ E+A LK +L Sbjct: 180 AKAEASKELSR-GPSASLDDELASLDEGSAVDDELAALKRQL 220 >Q8CV17.1 Q8CV17_OCEIH Phage shock protein A (Membrane protein) Length = 223 Score = 86.3 bits (212), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 66/224 (29%), Positives = 113/224 (50%), Gaps = 14/224 (6%) Query: 4 FRSMRDITVANLNERLEQSQDPVKLIDQFLHSTREEITEAERLYQQYAAHTKQLQQQVNQ 63 F ++ + + LN L++++DPVK++DQF+ E+I E E + ++ K L+++ + Sbjct: 5 FNRVKTVVSSELNSALDKAEDPVKMLDQFMRDMAEDIREVETAVAKQISNEKMLKRKADD 64 Query: 64 ATAMRNKREEQALLALKAGEDHLAKLALQEKIIHEEKXXXXXXXXXXXXXXXXXXXXXXX 123 A AM KR++QA A++AG D LA+ ALQ+K HE Sbjct: 65 AKAMMEKRQKQAEQAIEAGNDDLARRALQDKKEHEATATSLNESWTTAKNDAEVLKSKLD 124 Query: 124 XXKMEYQTVYSKRQYYAARMESLRLQQRMNQRATAYGNGSDVPKMFGRLEDRMSDWELEA 183 K EYQ + K+ AR ES + + ++N+ ++ GN S + F R+E+++ +E EA Sbjct: 125 EMKKEYQEMKLKKDSLKARAESAKTRTKINRTMSSIGNDS-SKQGFERMEEKVMRYEAEA 183 Query: 184 KS-------LRDLRRMGQEYAIQAGETVATVLEREMARLKEKLN 220 ++ R L +E Q G ++ E+A LK K+N Sbjct: 184 ETSEAMSYESRSLDDEFEELNKQNG------VDDELAALKAKMN 221 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.126 0.335 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 979,417,182 Number of extensions: 31473636 Number of successful extensions: 162631 Number of sequences better than 1.0e-05: 445 Number of HSP's gapped: 162385 Number of HSP's successfully gapped: 445 Length of query: 225 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 94 Effective length of database: 1,826,543,238 Effective search space: 171695064372 Effective search space used: 171695064372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)