BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0008.p (315 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z7C3.1 C0Z7C3_BREBN Conserved hypothetical membrane protein 238 5e-61 B4D4M1.1 B4D4M1_9BACT Putative uncharacterized protein 169 3e-40 B5JKM9.1 B5JKM9_9BACT Integral membrane protein DUF6 158 6e-37 Q02AY6.1 Q02AY6_SOLUE Putative uncharacterized protein 134 8e-30 C1AEB3.1 C1AEB3_GEMAT Hypothetical membrane protein 131 8e-29 >C0Z7C3.1 C0Z7C3_BREBN Conserved hypothetical membrane protein Length = 322 Score = 238 bits (607), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 128/312 (41%), Positives = 175/312 (56%), Gaps = 5/312 (1%) Query: 8 NPVYVIGIALVSVYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISRLSGAKRP 67 + ++ + IAL++VYLFWGGTY+GMK+AIE+MPPF+MAG RFF+AG++L++I R GA+ P Sbjct: 7 SKLFGVTIALLAVYLFWGGTYLGMKIAIESMPPFIMAGARFFLAGSILFLIGRWKGAELP 66 Query: 68 AGREWRSSAIVXXXXXXXXXXXVAWSEQRXXXXXXXXXXXXXXXWMMLFGWLSRSGKRPX 127 + EWR + IV VAW++ + W+++F W+ S K+P Sbjct: 67 SAAEWRGAGIVGALLLLGGNGVVAWAQLKVPSAIASLLIATVPLWILVFNWVGGSKKKPT 126 Query: 128 XXXXXXXXXXXXXXXXXXFQPGHKDNGTATDLTGIITXXXXXXXXXXXXMYSRSARMPDS 187 P N D GI +YSR+A++P S Sbjct: 127 VGVMGGILFGLAGIAVLVVHPESSGN-QGIDTVGIFALLFASISWAVGSLYSRNAKLPAS 185 Query: 188 PLMSTAAQMLTGGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFGSIIGYTAYIWL 247 P+M+TA QM+ GG LL I S F DW+KL + I+LRS+ A GYL+ FGSI+ YTAYIWL Sbjct: 186 PVMATALQMIIGGSLLGITSLFLDDWTKLHLSEITLRSWIAFGYLVGFGSIVAYTAYIWL 245 Query: 248 LKNADAALVSTYAFVNPVVAVFFGWLLAGEQLTVNXXXXXXXXXXXXXXXXXFRSRAVPS 307 LKNA+ +LVSTYA+VNP+VAVF GWL+A EQLT FR + P Sbjct: 246 LKNAEPSLVSTYAYVNPIVAVFLGWLIADEQLTSQTLIAAVMIIAAVAIITMFREKKSPG 305 Query: 308 AKMM----EQGR 315 A E+G+ Sbjct: 306 ATQTIARKEKGK 317 >B4D4M1.1 B4D4M1_9BACT Putative uncharacterized protein Length = 320 Score = 169 bits (428), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 97/268 (36%), Positives = 139/268 (51%), Gaps = 2/268 (0%) Query: 12 VIGIALVSVYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISRLSGAKRPAGRE 71 VIG A +VYL WG TY+G+++AIET+PPFLMAG RF VAG+VLY I R +G RP + Sbjct: 30 VIG-AFAAVYLIWGSTYLGIRLAIETIPPFLMAGARFVVAGSVLYTIMRRTGTPRPQAIQ 88 Query: 72 WRSSAIVXXXXXXXXXXXVAWSEQRXXXXXXXXXXXXXXXWMMLFGWLSRSGKRPXXXXX 131 WR + I+ V+W+E+R WM+L WL GKRP Sbjct: 89 WRDAVILGALLLLVGNGGVSWAEKRVPTNITALTVAGTPLWMLLLDWLRPGGKRPHGLVF 148 Query: 132 XXXXXXXXXXXXXXFQPGHKDNGTATDLTGIITXXXXXXXXXXXXMYSRSARMPDSPLMS 191 + +G D G + ++SR A+ P S L++ Sbjct: 149 AGLGLGFVGVALIVSSRDAQGHGI-MDPAGAVMLISASICWAAGSIFSRHAKQPSSALLA 207 Query: 192 TAAQMLTGGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFGSIIGYTAYIWLLKNA 251 + QM+ GG+L+L+ + + IS S A YL +FGS++G+T Y+WLL+ + Sbjct: 208 ISMQMIAGGLLMLLAGCVLGEAQRFHWHDISRTSAEAFVYLTIFGSLVGFTCYVWLLRVS 267 Query: 252 DAALVSTYAFVNPVVAVFFGWLLAGEQL 279 A VSTYA+VNP++AV G ++ GE L Sbjct: 268 TPARVSTYAYVNPLIAVVMGRVVLGEPL 295 >B5JKM9.1 B5JKM9_9BACT Integral membrane protein DUF6 Length = 290 Score = 158 bits (400), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 96/271 (35%), Positives = 139/271 (51%), Gaps = 2/271 (0%) Query: 12 VIGIALVSVYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISRLSGAKRPAGRE 71 +I +A ++YL WG TY+ +KVA+ETMPPFLMA RFF+AG VLY ++R SGA RP+G + Sbjct: 1 MICLAFAALYLIWGSTYLAIKVAVETMPPFLMATGRFFLAGLVLYGVARASGAARPSGGQ 60 Query: 72 WRSSAIVXXXXXXXXXXXVAWSEQRXXXXXXXXXXXXXXXWMMLFGWLSRSGKRPXXXXX 131 W+ S IV V+++E +M LFGW +P Sbjct: 61 WKHSWIVGGFLIAGGNGVVSYAETFIDSSMAALIIASNPLFMTLFGWWGGVQAKPGRMAW 120 Query: 132 XXXXXXXXXXXXXXFQPGHKDNGTATDLTGIITXXXXXXXXXXXXMYSRSARMPDSPLMS 191 + G L G I +YS+ +P + Sbjct: 121 LSLAGGFVGVATLIASGSGIEWGN--SLFGYILVVGCVLLWTVGSIYSKRNPQEMNPWLQ 178 Query: 192 TAAQMLTGGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFGSIIGYTAYIWLLKNA 251 + QM+ GG + L+ ++S LD AIS RS+ A GYL + GS+IG+T+Y++LLK+ Sbjct: 179 SGMQMICGGAICLLAGGSMGEFSTLDFAAISSRSWIAFGYLFIVGSLIGFTSYVYLLKHC 238 Query: 252 DAALVSTYAFVNPVVAVFFGWLLAGEQLTVN 282 + VS++A+VNPVVAV GWL+ GE L + Sbjct: 239 VPSTVSSHAYVNPVVAVILGWLILGETLNMG 269 >Q02AY6.1 Q02AY6_SOLUE Putative uncharacterized protein Length = 304 Score = 134 bits (338), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 83/267 (31%), Positives = 123/267 (46%), Gaps = 5/267 (1%) Query: 15 IALVSVYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISRLSGAKRPAGREWRS 74 +A ++Y WG T++ ++ A+ET+PP + G R +AG +L++ R G P R WR Sbjct: 20 LAFAAIYFVWGSTFLAIRFAVETLPPLITMGTRHLMAGGLLFLWLRFRGEPLPEPRLWRP 79 Query: 75 SAIVXXXXXXXXXXXVAWSEQRXXXXXXXXXXXXXXXWMMLFG-WLSRSGKRPXXXXXXX 133 + +AW+E+ WM+L L + + Sbjct: 80 ALFSGAFCFLGCHGLLAWAEKTVPSGLAALLSATLPVWMILLARMLGQGSELTLKVMGGI 139 Query: 134 XXXXXXXXXXXXFQPGHKDNGTATDLTGIITXXXXXXXXXXXXMYSRSARMPDSPLMSTA 193 F G +D T L I+ ++SR + P A Sbjct: 140 TLGFLGVAVLVPFHSGARDGIPWTSLAIIVCEIFWAIGA----IFSRGVKGTVPPATFAA 195 Query: 194 AQMLTGGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFGSIIGYTAYIWLLKNADA 253 QML GGVLL + S+L + + RS +L YLIVFGS+I +TAY+WLL+ Sbjct: 196 MQMLCGGVLLWTLGLILGEGSQLHPESFTTRSVVSLAYLIVFGSLITFTAYVWLLQVCSP 255 Query: 254 ALVSTYAFVNPVVAVFFGWLLAGEQLT 280 ALVST++++NPVVA+F GW L GE LT Sbjct: 256 ALVSTHSYINPVVAIFCGWALGGEPLT 282 >C1AEB3.1 C1AEB3_GEMAT Hypothetical membrane protein Length = 311 Score = 131 bits (329), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 121/261 (46%), Gaps = 5/261 (1%) Query: 20 VYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISRLSGAKRPAGREWRSSAIVX 79 +Y+ WG TY+ +K + T+PPF+M RF +AG LY R GA RP EWR+SA++ Sbjct: 27 IYVVWGSTYLAIKWGVATIPPFVMGAARFLLAGGALYAFMRARGAPRPTLPEWRASAVIG 86 Query: 80 XXXXXXXXXXVAWSEQRXXXXXXXXXXXXXXXWMMLF-GWLSRSGKRPXXXXXXXXXXXX 138 V+W+ QR W++L W R P Sbjct: 87 ALLLFVGNGAVSWASQRVNSGLTSVIVATVPLWLVLCEAWQGR----PPRLAQVIGVAIG 142 Query: 139 XXXXXXXFQPGHKDNGTATDLTGIITXXXXXXXXXXXXMYSRSARMPDSPLMSTAAQMLT 198 P + A D G +YSR+A+ S ++ A QML Sbjct: 143 LVGVGLLVLPVGGERQAAVDPIGAAVLATGALSWTVGSLYSRTAKQSSSAALAIAMQMLI 202 Query: 199 GGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFGSIIGYTAYIWLLKNADAALVST 258 GG +L+ S +W ++ ++S S +L YL FGS+IG++ Y+WLLK A V T Sbjct: 203 GGACMLVLSLVLGEWQRVSPESVSTASLLSLLYLATFGSLIGFSTYMWLLKVASPTAVGT 262 Query: 259 YAFVNPVVAVFFGWLLAGEQL 279 YA+VNPVVAV G L GE+L Sbjct: 263 YAYVNPVVAVLLGVALGGERL 283 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,408,930,084 Number of extensions: 45839703 Number of successful extensions: 143341 Number of sequences better than 1.0e-05: 338 Number of HSP's gapped: 143265 Number of HSP's successfully gapped: 487 Length of query: 315 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 180 Effective length of database: 1,788,859,178 Effective search space: 321994652040 Effective search space used: 321994652040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)