BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0002.p (451 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4ILN9.1 C4ILN9_CLOBU Bacterial solute-binding protein 1 family 437 e-120 B1QVY0.1 B1QVY0_CLOBU Bacterial extracellular solute-binding pro... 437 e-120 B2UYQ0.1 B2UYQ0_CLOBA Putative glycerol-3-phosphate ABC transporter 431 e-119 B2TQC1.1 B2TQC1_CLOBB Putative glycerol-3-phosphate ABC transpor... 431 e-118 A7B8D1.1 A7B8D1_RUMGN Putative uncharacterized protein 427 e-117 >C4ILN9.1 C4ILN9_CLOBU Bacterial solute-binding protein 1 family Length = 442 Score = 437 bits (1124), Expect = e-120, Method: Compositional matrix adjust. Identities = 220/454 (48%), Positives = 295/454 (64%), Gaps = 17/454 (3%) Query: 2 MMNVLKRLSLVSMIAGLT-VITACGGNSASDNXXXXXXXXXXXXXXXXXPTQGKPVTIEF 60 M +K++ + +I+ +T + +CG + + T + IE+ Sbjct: 1 MNKFIKKICVAGLISVMTGSMMSCGTKTEN-------------KVAEATKTSDGKIQIEY 47 Query: 61 WYGLGGKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPA-AVLSS 119 WYGLGGKL ENM+ +++ FN+SQ + VK I Q DY T + LQAAI+ + PA A+L Sbjct: 48 WYGLGGKLDENMQRIVRDFNSSQDKYEVKPIAQDDYDTTYKNLQAAISAKKTPALALLEP 107 Query: 120 NMDWA--RKGYFASMDELIAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQVMYYRK 177 + + K ++E+ + ++N ++++ F++ G+ DGKQY P+YGTTQ++YY Sbjct: 108 DKQYTLGSKDLLTDINEIASKDSEYNADNYLSAFIEPGKQDGKQYAFPIYGTTQLLYYNT 167 Query: 178 DAFEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFGWEPMWGSGNMIDAVMSKGGSIL 237 AF+ +GI LKTW LA AA K+ +DG + +F+GWEPM G N++DA +S GG +L Sbjct: 168 KAFKDAGISEDSLKTWSGLADAASKLVKKDGDEVSFYGWEPMKGPDNLVDAALSNGGQVL 227 Query: 238 SGDGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTIDDVMKGQAAGYTG 297 S D TKV I+S EWVE WD FRKWIHEDKIMRIHSGGQGW+YW T+ D M+ + AGYTG Sbjct: 228 SDDKTKVMINSDEWVEAWDSFRKWIHEDKIMRIHSGGQGWQYWNDTVADAMEQKTAGYTG 287 Query: 298 SSGDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAEKQAAMKWLTYFTNS 357 S+GDQGDLDF+I+SA EQP + E KPVAEA IP D EKQ A +++ YFT+ Sbjct: 288 SAGDQGDLDFNILSAYEQPAFKEGKESKPVAEARFLVIPKNVSDEEKQGAYEFIKYFTSP 347 Query: 358 ENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHASAPFQDPTGGKISDA 417 + A WSI +GYI+V + + DPAF + P VP MQA+HAS F DPTGGKISDA Sbjct: 348 KVNAAWSIASGYIAVNKESPNDPAFKEYVNDKPYALVPTMQAAHASQAFIDPTGGKISDA 407 Query: 418 LKIAADKVQIENTPAAEALKEAQQTAQAALDKMK 451 LKIAADKV+IEN PA EAL EAQ+TAQ ALD +K Sbjct: 408 LKIAADKVEIENVPAKEALDEAQKTAQKALDAVK 441 >B1QVY0.1 B1QVY0_CLOBU Bacterial extracellular solute-binding protein Length = 442 Score = 437 bits (1124), Expect = e-120, Method: Compositional matrix adjust. Identities = 220/454 (48%), Positives = 295/454 (64%), Gaps = 17/454 (3%) Query: 2 MMNVLKRLSLVSMIAGLT-VITACGGNSASDNXXXXXXXXXXXXXXXXXPTQGKPVTIEF 60 M +K++ + +I+ +T + +CG + + T + IE+ Sbjct: 1 MNKFIKKICVAGLISVMTGSMMSCGTKTEN-------------KVAEATKTSDGKIQIEY 47 Query: 61 WYGLGGKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPA-AVLSS 119 WYGLGGKL ENM+ +++ FN+SQ + VK I Q DY T + LQAAI+ + PA A+L Sbjct: 48 WYGLGGKLDENMQRIVRDFNSSQDKYEVKPIAQDDYDTTYKNLQAAISAKKTPALALLEP 107 Query: 120 NMDWA--RKGYFASMDELIAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQVMYYRK 177 + + K ++E+ + ++N ++++ F++ G+ DGKQY P+YGTTQ++YY Sbjct: 108 DKQYTLGSKDLLTDINEIASKDSEYNADNYLSAFIEPGKQDGKQYAFPIYGTTQLLYYNT 167 Query: 178 DAFEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFGWEPMWGSGNMIDAVMSKGGSIL 237 AF+ +GI LKTW LA AA K+ +DG + +F+GWEPM G N++DA +S GG +L Sbjct: 168 KAFKDAGISEDSLKTWSGLADAASKLVKKDGDEVSFYGWEPMKGPDNLVDAALSNGGQVL 227 Query: 238 SGDGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTIDDVMKGQAAGYTG 297 S D TKV I+S EWVE WD FRKWIHEDKIMRIHSGGQGW+YW T+ D M+ + AGYTG Sbjct: 228 SDDKTKVMINSDEWVEAWDSFRKWIHEDKIMRIHSGGQGWQYWNDTVADAMEQKTAGYTG 287 Query: 298 SSGDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAEKQAAMKWLTYFTNS 357 S+GDQGDLDF+I+SA EQP + E KPVAEA IP D EKQ A +++ YFT+ Sbjct: 288 SAGDQGDLDFNILSAYEQPAFKEGKESKPVAEARFLVIPKNVSDEEKQGAYEFIKYFTSP 347 Query: 358 ENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHASAPFQDPTGGKISDA 417 + A WSI +GYI+V + + DPAF + P VP MQA+HAS F DPTGGKISDA Sbjct: 348 KVNAAWSIASGYIAVNKESPNDPAFKEYVNDKPYALVPTMQAAHASQAFIDPTGGKISDA 407 Query: 418 LKIAADKVQIENTPAAEALKEAQQTAQAALDKMK 451 LKIAADKV+IEN PA EAL EAQ+TAQ ALD +K Sbjct: 408 LKIAADKVEIENVPAKEALDEAQKTAQKALDAVK 441 >B2UYQ0.1 B2UYQ0_CLOBA Putative glycerol-3-phosphate ABC transporter Length = 439 Score = 431 bits (1108), Expect = e-119, Method: Compositional matrix adjust. Identities = 209/397 (52%), Positives = 273/397 (68%), Gaps = 3/397 (0%) Query: 56 VTIEFWYGLGGKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPAA 115 + +E+WYGLGGKLG+NM+++I FN SQ + VKGI Q DY T + LQAAIA + PA Sbjct: 39 IKVEYWYGLGGKLGKNMDNIINDFNNSQDKYEVKGISQEDYDATFKNLQAAIAAKKTPAL 98 Query: 116 VLSSNMDWA---RKGYFASMDELIAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQV 172 V+ A KG A + A +FN ++++++F + + D K Y PM+GTTQV Sbjct: 99 VVLEPDKGAILNNKGALADISNYTAEDSNFNSDNYIKSFFESCKKDNKLYAFPMFGTTQV 158 Query: 173 MYYRKDAFEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFGWEPMWGSGNMIDAVMSK 232 +YY K FE +GI L TW +A A+KK+ +DG +T F+GWEPMWG+ N+IDA +S Sbjct: 159 LYYNKKEFENAGISKDSLNTWSKIAEASKKVIKKDGDETKFYGWEPMWGADNLIDASLSN 218 Query: 233 GGSILSGDGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTIDDVMKGQA 292 GGSILS DG+KV I+SPEW+E W+ FR WIH+DKIM+I+S GQGWEYWY TIDDVM+ +A Sbjct: 219 GGSILSEDGSKVLINSPEWIEVWENFRMWIHDDKIMKINSSGQGWEYWYSTIDDVMQDRA 278 Query: 293 AGYTGSSGDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAEKQAAMKWLT 352 GYTGSSGDQGDLDF + A+EQP + E KP+AEA + IP A + K+ A +++ Sbjct: 279 GGYTGSSGDQGDLDFEKLGAIEQPAFKEGKEAKPIAEARLLVIPKLANEEAKKGAYEFIK 338 Query: 353 YFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHASAPFQDPTGG 412 YFT+ E A WSI +GYI V ++A AF + E NPQ VP+ QA HAS F DPT G Sbjct: 339 YFTSPEVNAKWSIASGYIPVNKAACNTDAFKEYVEKNPQALVPITQALHASKSFIDPTNG 398 Query: 413 KISDALKIAADKVQIENTPAAEALKEAQQTAQAALDK 449 KI DALK+AADKV++EN A EAL EA++ AQ+ LDK Sbjct: 399 KIYDALKVAADKVELENISAKEALDEAEKIAQSELDK 435 >B2TQC1.1 B2TQC1_CLOBB Putative glycerol-3-phosphate ABC transporter, glycerol-3-phosphate-binding protein Length = 440 Score = 431 bits (1107), Expect = e-118, Method: Compositional matrix adjust. Identities = 208/397 (52%), Positives = 271/397 (68%), Gaps = 3/397 (0%) Query: 56 VTIEFWYGLGGKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPAA 115 + +E+WYGLGGKLG+NM+++I FN SQ + VKGI Q DY T + LQAAIA + P Sbjct: 39 IKVEYWYGLGGKLGKNMDNIINDFNNSQDKYEVKGISQEDYDATFKNLQAAIAAKKTPTL 98 Query: 116 VLSSNMDWA---RKGYFASMDELIAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQV 172 V+ A KG A + A DFN ++++++F + + D K Y PM+GTTQV Sbjct: 99 VVLEPDKGAILNNKGALADISNYTAEDSDFNSDNYIKSFFESCKKDNKLYAFPMFGTTQV 158 Query: 173 MYYRKDAFEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFGWEPMWGSGNMIDAVMSK 232 +YY K E +GI L TW +A A+KK+ +DG +T F+GWEPMWG+ N+IDA +S Sbjct: 159 LYYNKKELENAGISKDSLNTWSKIAEASKKLIKKDGDETKFYGWEPMWGADNLIDASLSN 218 Query: 233 GGSILSGDGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTIDDVMKGQA 292 GGSILS DG++V I+SPEWVE WD FR WIH+DKIM+++S GQGWEYWY TIDDVM+ +A Sbjct: 219 GGSILSEDGSRVLINSPEWVEVWDNFRLWIHDDKIMKVNSSGQGWEYWYSTIDDVMQDRA 278 Query: 293 AGYTGSSGDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAEKQAAMKWLT 352 GYTGSSGDQGDLDF + A+EQP + E KP+AEA + IP A + K+ A +++ Sbjct: 279 GGYTGSSGDQGDLDFEKLGAIEQPAFKDGKEAKPIAEARLLVIPNLANEEAKKGAYEFIK 338 Query: 353 YFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHASAPFQDPTGG 412 YFT+ E A WSI +GYI V ++A AF + E NPQ VP+ QA HAS F DPT G Sbjct: 339 YFTSPEVNAKWSIASGYIPVNKAACNTDAFKEYVEKNPQALVPITQALHASKSFIDPTNG 398 Query: 413 KISDALKIAADKVQIENTPAAEALKEAQQTAQAALDK 449 KI DALK+AADKV++EN A EAL EA++ AQ+ LDK Sbjct: 399 KIYDALKVAADKVELENVSAQEALDEAEKIAQSELDK 435 >A7B8D1.1 A7B8D1_RUMGN Putative uncharacterized protein Length = 433 Score = 427 bits (1097), Expect = e-117, Method: Compositional matrix adjust. Identities = 222/451 (49%), Positives = 294/451 (65%), Gaps = 27/451 (5%) Query: 7 KRL--SLVSMIAGLTVITACGGNSASDNXXXXXXXXXXXXXXXXXPTQGKPVTIEFWYGL 64 KR+ +L+ + G++++T CG N+ + T + ++ WY Sbjct: 3 KRIVSALLVCVLGMSLVTGCGNNAKA---------------AETTKTADGKIQLDLWYS- 46 Query: 65 GGKLGENM-ESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPAAVL---SSN 120 GGK N+ + ++ FN SQ + VK QADY+ET +KLQA IA + P L + Sbjct: 47 GGKTAVNVFQDIVDAFNESQDKYEVKTTTQADYTETYEKLQAGIAGKKAPDMALLDTDKS 106 Query: 121 MDWARKGYFASMDELIAAQPDFNKEDFVQTFLKQGQ-VDGKQYFLPMYGTTQVMYYRKDA 179 + + K A ++E + F K+D++ F +QG+ +GK + LP YGTTQVMYY K A Sbjct: 107 RNLSEKNLVADINEYVEKDDSFEKDDYLPVFYEQGEDENGKLFALPAYGTTQVMYYNKAA 166 Query: 180 FEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFGWEPMWGSGNMIDAVMSKGGSILSG 239 FEK+GID + +KTW+DLAAAA+KM DG +F GWEPMWGSGN+IDA +S G S+LS Sbjct: 167 FEKAGIDPATIKTWQDLAAAAEKMTASDG---SFVGWEPMWGSGNLIDAALSNGASLLSE 223 Query: 240 DGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTIDDVMKGQAAGYTGSS 299 DG KV I+S EWVE W+ FR WIH+DKIM+++SGGQGWEYWY TIDDV++ +A GYTGSS Sbjct: 224 DGKKVMINSDEWVEVWESFRTWIHDDKIMKVNSGGQGWEYWYTTIDDVLQNKAGGYTGSS 283 Query: 300 GDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAEKQAAMKWLTYFTNSEN 359 GDQ DLDFSIV AMEQPG+ P A+AL + + D EK+ +++ YFT EN Sbjct: 284 GDQADLDFSIVGAMEQPGFND-NPSAPTADALQLVMLQNSSDEEKEGVYEFMKYFTTPEN 342 Query: 360 TAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHASAPFQDPTGGKISDALK 419 A WS+ TGY++VR+S + F S++E NPQ VPL QASH + QDPTGGKI DAL Sbjct: 343 QAKWSMGTGYVAVRESTQEVEEFKSYAEENPQALVPLQQASHGTPALQDPTGGKILDALS 402 Query: 420 IAADKVQIENTPAAEALKEAQQTAQAALDKM 450 IAADKV++EN PA EAL EAQ+TAQ ALD + Sbjct: 403 IAADKVELENVPAQEALDEAQKTAQEALDAL 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.129 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,618,739,924 Number of extensions: 108783474 Number of successful extensions: 232195 Number of sequences better than 1.0e-05: 1307 Number of HSP's gapped: 231320 Number of HSP's successfully gapped: 1315 Length of query: 451 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 312 Effective length of database: 1,751,175,118 Effective search space: 546366636816 Effective search space used: 546366636816 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)