BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0031.p (394 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KHQ4.1 B3KHQ4_9BACI Amidohydrolase EC=3.5.1.14 507 e-142 B1D6E1.1 B1D6E1_9BACL Amidohydrolase EC=3.5.1.32 503 e-140 B7JI99.1 B7JI99_BACC0 Thermostable carboxypeptidase 1 EC=3.4.17.- 463 e-128 C3C5T8.1 C3C5T8_BACTU Putative uncharacterized protein 460 e-127 B5V6X6.1 B5V6X6_BACCE Thermostable carboxypeptidase 1 EC=3.4.17.- 457 e-127 >B3KHQ4.1 B3KHQ4_9BACI Amidohydrolase EC=3.5.1.14 Length = 391 Score = 507 bits (1306), Expect = e-142, Method: Compositional matrix adjust. Identities = 257/374 (68%), Positives = 299/374 (79%) Query: 16 EKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTGVIAEIGGLQDGPVV 75 ++ I IRRHLH++PELSNEEFETT+ I LE+AGI + Y L TGVIAE+GG Q+GP++ Sbjct: 13 QRFIEIRRHLHRYPELSNEEFETTQLIRGWLEEAGIRVAGYPLGTGVIAEVGGFQEGPII 72 Query: 76 ALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKGKVRLL 135 ALRADIDALP+ EETGLPYAS PGK HACGHDFHTAAL+G AY LKQ+E +L+G VRL+ Sbjct: 73 ALRADIDALPVHEETGLPYASTIPGKMHACGHDFHTAALIGTAYALKQREQELRGTVRLI 132 Query: 136 FQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFVVEVRG 195 FQPAEEKAKGA+Q+I SGALEGV+A+ GMHNKPDLPVGT+GI GPLMAAADGFVVEV G Sbjct: 133 FQPAEEKAKGAKQVIDSGALEGVQAIFGMHNKPDLPVGTIGIKEGPLMAAADGFVVEVAG 192 Query: 196 AGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNIIPETAV 255 GSHAAVPEAG DPIV ASHI+TALQSIVSRNVS L+SAV+SVTKLHSG +WN+IP+ A+ Sbjct: 193 KGSHAAVPEAGLDPIVTASHIITALQSIVSRNVSPLKSAVISVTKLHSGTAWNVIPDKAL 252 Query: 256 LEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPVNNDAGLARLGEETA 315 LEGTIRTF++ VR +VLERF RW+ GPPPV+ND LA LG A Sbjct: 253 LEGTIRTFDDDVRHQVLERFGQVVQGVAAAFGATAAVRWIEGPPPVHNDRKLAELGYAAA 312 Query: 316 ATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSREWHHPAFNLDEAALPVGAAFF 375 A G++ + P PSPAGEDFA YQR VPGLFVF+GTAG++EWHHPAF+LDE A+PV FF Sbjct: 313 AEAGYEAVIPVPSPAGEDFAVYQREVPGLFVFMGTAGTQEWHHPAFDLDERAIPVSIDFF 372 Query: 376 AGLAVRVLEHLSAG 389 LA R L H AG Sbjct: 373 TRLAERALRHYHAG 386 >B1D6E1.1 B1D6E1_9BACL Amidohydrolase EC=3.5.1.32 Length = 395 Score = 503 bits (1296), Expect = e-140, Method: Compositional matrix adjust. Identities = 256/387 (66%), Positives = 295/387 (76%), Gaps = 2/387 (0%) Query: 2 MSIRNAEHGAQALNEKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTG 61 MS A AL ++ IRR LH++PELS+EEFETT I SLLE GI I DY L TG Sbjct: 1 MSNDTTTRSAAALAGRLTDIRRELHRNPELSHEEFETTIRIKSLLEAGGIRIADYPLKTG 60 Query: 62 VIAEIGGLQDGPVVALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQL 121 V+AEIG PV+ALRADIDALPIQEETGLP+AS PGK HACGHDFHTAAL+GAAY L Sbjct: 61 VVAEIG--SGTPVIALRADIDALPIQEETGLPFASAVPGKMHACGHDFHTAALIGAAYLL 118 Query: 122 KQQEPDLKGKVRLLFQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGP 181 K +E +LKG VRL+FQPAEEKAKGA+Q+I SGALEGV+A+ G+HNKPDLPVGT+GI GGP Sbjct: 119 KDRERELKGTVRLIFQPAEEKAKGARQVIDSGALEGVQAIFGLHNKPDLPVGTIGIKGGP 178 Query: 182 LMAAADGFVVEVRGAGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKL 241 LMAAADGFVVEV G GSHAAVPEAG DP++ A+HIVTALQSIVSRNV AL SAV+SVTKL Sbjct: 179 LMAAADGFVVEVAGRGSHAAVPEAGNDPVLTAAHIVTALQSIVSRNVGALDSAVISVTKL 238 Query: 242 HSGNSWNIIPETAVLEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPV 301 +SG +WN+IPE AVL+GTIRTF+ +R +V ERF RW+ GPPPV Sbjct: 239 NSGTAWNVIPEKAVLDGTIRTFDPDIRRRVRERFDQVVAGVAAAFDTTAVVRWMEGPPPV 298 Query: 302 NNDAGLARLGEETAATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSREWHHPAF 361 +ND GLA L + A L + P PS AGEDFA YQ++VPGLFVFVGT G +EWHHPAF Sbjct: 299 HNDEGLAELAWQEAVQLELLPVSPVPSLAGEDFAAYQQLVPGLFVFVGTDGPKEWHHPAF 358 Query: 362 NLDEAALPVGAAFFAGLAVRVLEHLSA 388 +LDE ALPV A F AG A+R L H +A Sbjct: 359 DLDERALPVAADFLAGTAIRALSHFAA 385 >B7JI99.1 B7JI99_BACC0 Thermostable carboxypeptidase 1 EC=3.4.17.- Length = 381 Score = 463 bits (1191), Expect = e-128, Method: Compositional matrix adjust. Identities = 219/376 (58%), Positives = 276/376 (73%) Query: 12 QALNEKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTGVIAEIGGLQD 71 + L E +I IRR+LH+HPELS EEFETT+ I + LE+ ITII+ L TGVIAEI G ++ Sbjct: 6 EQLTETLISIRRNLHEHPELSYEEFETTKAIKNWLEEENITIINSNLETGVIAEISGNRN 65 Query: 72 GPVVALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKGK 131 GP++A+RADIDALPIQEET LPYAS GK HACGHDFHTAA++GAAY LK++E L G Sbjct: 66 GPLIAIRADIDALPIQEETNLPYASKIHGKMHACGHDFHTAAIIGAAYLLKEKESSLSGT 125 Query: 132 VRLLFQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFVV 191 VR +FQPAEE + GA ++I +G L GV+A+ GMHNKPDLPVGT+GI GPLMA D F + Sbjct: 126 VRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHNKPDLPVGTIGIKDGPLMAGVDRFEI 185 Query: 192 EVRGAGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNIIP 251 E+ G G+HAAVP+AG DPIVA+S IV ALQ+IVSRN+S+ +AVVSVT +HSGN+WN+IP Sbjct: 186 EIHGVGTHAAVPDAGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNTWNVIP 245 Query: 252 ETAVLEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPVNNDAGLARLG 311 E A LEGT+RTF+ R K+ R+ GPP V+ND L L Sbjct: 246 EKATLEGTVRTFQTETREKIPALMKRIIQGVSDALGVKTEFRFYAGPPAVHNDTSLTNLS 305 Query: 312 EETAATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSREWHHPAFNLDEAALPVG 371 + A T+ + + P+PS AGEDF+FYQ+ +PG FVF+GT+G+ EWHHPAF LDE ALP+ Sbjct: 306 TQVAETMNLNIISPTPSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTLDERALPIS 365 Query: 372 AAFFAGLAVRVLEHLS 387 AA+FA LA + L+H S Sbjct: 366 AAYFALLAEKALKHFS 381 >C3C5T8.1 C3C5T8_BACTU Putative uncharacterized protein Length = 381 Score = 460 bits (1183), Expect = e-127, Method: Compositional matrix adjust. Identities = 217/376 (57%), Positives = 275/376 (73%) Query: 12 QALNEKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTGVIAEIGGLQD 71 + L E +I IRR+LH+HPELS EEFETT+ I + LE+ ITII L TG+IAEI G ++ Sbjct: 6 EQLTETLISIRRNLHEHPELSYEEFETTKAIKNWLEEENITIIHSNLETGIIAEISGNRN 65 Query: 72 GPVVALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKGK 131 GP++A+RADIDALPIQEET LPYAS GK HACGHDFHTAA++GAAY LK++E L G Sbjct: 66 GPLIAIRADIDALPIQEETNLPYASKIHGKMHACGHDFHTAAIIGAAYLLKEKESSLSGT 125 Query: 132 VRLLFQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFVV 191 VR +FQPAEE + GA ++I +G L GV+A+ GMHNKPDLPVGT+GI GPLMA D F + Sbjct: 126 VRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHNKPDLPVGTIGIKDGPLMAGVDRFEI 185 Query: 192 EVRGAGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNIIP 251 E+ G G+HAAVP+AG DPIVA+S IV ALQ+IVSRN+S+ +AVVSVT +HSGN+WN+IP Sbjct: 186 EIHGVGTHAAVPDAGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNTWNVIP 245 Query: 252 ETAVLEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPVNNDAGLARLG 311 E A LEGT+RTF+ R K+ R+ GPP V+ND L L Sbjct: 246 EKATLEGTVRTFQTETREKIPALMKRIIQGVSDALGVKTEFRFYAGPPAVHNDTSLTNLS 305 Query: 312 EETAATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSREWHHPAFNLDEAALPVG 371 + A T+ + + P+PS AGEDF+FYQ+ +PG FVF+GT+G+ EWHHPAF +DE ALP+ Sbjct: 306 TQVAETMNLNIISPTPSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTVDERALPIS 365 Query: 372 AAFFAGLAVRVLEHLS 387 AA+FA LA + L+H S Sbjct: 366 AAYFALLAEKALKHFS 381 >B5V6X6.1 B5V6X6_BACCE Thermostable carboxypeptidase 1 EC=3.4.17.- Length = 381 Score = 457 bits (1177), Expect = e-127, Method: Compositional matrix adjust. Identities = 216/376 (57%), Positives = 273/376 (72%) Query: 12 QALNEKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTGVIAEIGGLQD 71 + L E +I IRR+LH+HPELS EEFETT+ I + LE+ ITII+ L TGVIAEI G ++ Sbjct: 6 EQLTETLISIRRNLHEHPELSYEEFETTKAIKNWLEEKNITIINSNLETGVIAEISGNRN 65 Query: 72 GPVVALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKGK 131 GP++A+RADIDALPIQEET L YAS GK HACGHDFHTAA++GAAY LK++E L G Sbjct: 66 GPLIAIRADIDALPIQEETNLSYASKIHGKMHACGHDFHTAAIIGAAYLLKEKESSLSGT 125 Query: 132 VRLLFQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFVV 191 VR +FQPAEE + GA +I +G L GV+A+ GMHNKPDLPVGT+GI GPLMA D F + Sbjct: 126 VRFIFQPAEESSNGACNVIEAGHLRGVQAIFGMHNKPDLPVGTIGIKDGPLMAGVDRFEI 185 Query: 192 EVRGAGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNIIP 251 E+ G G+HAAVP+AG DPIVA+S IV ALQ+IVSRN+S+ +AVVSVT +HSGN+WN+IP Sbjct: 186 EIHGVGTHAAVPDAGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNTWNVIP 245 Query: 252 ETAVLEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPVNNDAGLARLG 311 E A LEGT+RTF+ R K+ R+ GPP V+ND L L Sbjct: 246 EKATLEGTVRTFQTETREKIPALMKRIIQGVSDALGVKTEFRFYAGPPAVHNDTSLTNLS 305 Query: 312 EETAATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSREWHHPAFNLDEAALPVG 371 + A T+ + + P+PS AGEDF+FYQ+ +PG FVF+GT+G+ EWHHP+F +DE ALP+ Sbjct: 306 TQVAETMNLNIISPTPSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPSFTVDERALPIS 365 Query: 372 AAFFAGLAVRVLEHLS 387 A +FA LA R L+H S Sbjct: 366 AEYFALLAERALKHFS 381 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,305,533,357 Number of extensions: 97190845 Number of successful extensions: 266658 Number of sequences better than 1.0e-05: 3126 Number of HSP's gapped: 257122 Number of HSP's successfully gapped: 3129 Length of query: 394 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 256 Effective length of database: 1,760,596,133 Effective search space: 450712610048 Effective search space used: 450712610048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)