BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0030.p (376 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9U6U1.1 A9U6U1_PHYPA Predicted protein (Fragment) 387 e-106 C1P8B3.1 C1P8B3_BACCO FAD dependent oxidoreductase 296 3e-78 P40859.2 MSOX_BACB0 Monomeric sarcosine oxidase EC=1.5.3.1 290 2e-76 P23342.1 MSOX_BACSN Monomeric sarcosine oxidase EC=1.5.3.1 283 2e-74 Q6ITC6.2 Q6ITC6_9BACI Monomeric sarcosine oxidase EC=1.5.3.1 281 5e-74 >A9U6U1.1 A9U6U1_PHYPA Predicted protein (Fragment) Length = 538 Score = 387 bits (994), Expect = e-106, Method: Compositional matrix adjust. Identities = 187/296 (63%), Positives = 227/296 (76%), Gaps = 1/296 (0%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 +GMSAGYYLAR+GVKTLL+DAFDPPH EGSHHGE RLIRHAYSGDP Y ++A+RAH LW Sbjct: 243 VGMSAGYYLARRGVKTLLIDAFDPPHQEGSHHGEPRLIRHAYSGDPVYTEMAIRAHRLWN 302 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSFADRLAEAEKRQVRVERLDADEIRRRWPVLNLPE 120 E+E + T+LLV SGVLNLAD+ +YS+ +R+ A++ + E LDA EIRRRWP L +P+ Sbjct: 303 EIEDLTETKLLVQSGVLNLADRGLYSYRERIELAKRHGLHPEWLDAAEIRRRWPELRVPD 362 Query: 121 HFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYHG 180 F AMYEP AGYL+SE+C+ YRKLAL+ GA+ + VTV T+ G Y+ Sbjct: 363 SFEAMYEPAAGYLHSERCVGAYRKLALSKGAELLTYAKVRGIEAHQDGVTVSTKEGTYYA 422 Query: 181 AAAILSAGAWFGSLPPFAALPIQAIRKVVGWFQS-TPDFDAGSFPGFTLGTGEGGYYGFP 239 + AILSAGAWFG+L PF LP++A+RK VGWF++ TP FD G FPGFTL EG YYGFP Sbjct: 423 SFAILSAGAWFGTLSPFVNLPVRAVRKTVGWFETNTPAFDNGRFPGFTLNAPEGSYYGFP 482 Query: 240 SIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLLKGS 295 SIGG GLKIGRHDTG W+PGE LAPFGS DEGDLRR L+AY+PGA+GRL +G+ Sbjct: 483 SIGGDGLKIGRHDTGHAWQPGEELAPFGSHPEDEGDLRRALDAYMPGASGRLKRGA 538 >C1P8B3.1 C1P8B3_BACCO FAD dependent oxidoreductase Length = 381 Score = 296 bits (757), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 162/363 (44%), Positives = 218/363 (60%), Gaps = 4/363 (1%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGY+LARQGVKTLL+DAFDPPHT GSHHGETR+IRHAY Y+ L LRA LW Sbjct: 15 MGMAAGYFLARQGVKTLLLDAFDPPHTSGSHHGETRIIRHAYGEGRDYVPLVLRAQTLWD 74 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSFADRLAE-AEKRQVRVERLDADEIRRRWPVLNLP 119 ELE+ SG ++ +GVL+ + + +F + E A + ++ + E+ +RWP ++LP Sbjct: 75 ELEKASGEKIFAKTGVLSFSPEDHAAFLNEAIESANTYNLPIKMMTGAEVEKRWPGISLP 134 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 F A+YEPDAG L+ E CI YRKLA A GA+ +VTV T++G + Sbjct: 135 AGFQAIYEPDAGVLFPENCIRAYRKLAEAKGARLLANTRVTDLKTDGQTVTVVTETGTFT 194 Query: 180 GAAAILSAGAWFGSLPPFAAL--PIQAIRKVVGWFQSTPD-FDAGSFPGFTLGTGEGGYY 236 I+S GAW G L L P+Q +RK VGWF++ F+A FP FT+ EG YY Sbjct: 195 AEKCIVSGGAWNGKLLENLGLNLPLQPVRKTVGWFEADEALFNADVFPAFTMDGYEGTYY 254 Query: 237 GFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLLKGSV 296 GFPS G+G+K+GRHD G+ P FG+ DEGD+R L+ Y+ GAAGRL KG V Sbjct: 255 GFPSFNGSGVKVGRHDGGQAADPDTMNREFGAYREDEGDIRAFLKTYMRGAAGRLKKGKV 314 Query: 297 CKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNIGPFS 356 C Y +TPDE FIID HP Y ++ +A E+L+ + ++ + FS Sbjct: 315 CLYTNTPDEHFIIDTHPVYPNVAIAAGFSGHGFKFASSVGEMLSQMVLKENAESPLPLFS 374 Query: 357 LSR 359 ++R Sbjct: 375 INR 377 >P40859.2 MSOX_BACB0 Monomeric sarcosine oxidase EC=1.5.3.1 Length = 390 Score = 290 bits (741), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 157/367 (42%), Positives = 216/367 (58%), Gaps = 11/367 (2%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGY LA+QGVKTLLVDAFDPPHT GSHHG+TR+IRHAY Y+ LALR+ LW Sbjct: 15 MGMAAGYQLAKQGVKTLLVDAFDPPHTNGSHHGDTRIIRHAYGEGREYVPLALRSQELWY 74 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSF-ADRLAEAEKRQVRVERLDADEIRRRWPVLNLP 119 ELE+E+ ++ +GVL + +F A+ + A++ + V+ L+ DEI +RWP + +P Sbjct: 75 ELEKETHHKIFTKTGVLVFGPKGESAFVAETMEAAKEHSLTVDLLEGDEINKRWPGITVP 134 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 E++ A++EP++G L+SE CI YR+LA A GA+ SV + T +G Y Sbjct: 135 ENYNAIFEPNSGVLFSENCIRAYRELAEARGAKVLTHTRVEDFDISPDSVKIETANGSYT 194 Query: 180 GAAAILSAGAWFGSLPPFAAL--PIQAIRKVVGWFQS-----TPDFDAGSFPGFTLGTGE 232 I+S GAW L L P+Q R+VVG+F+S + D D FPGF + Sbjct: 195 ADKLIVSMGAWNSKLLSKLNLDIPLQPYRQVVGFFESDESKYSNDID---FPGFMVEVPN 251 Query: 233 GGYYGFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLL 292 G YYGFPS GG GLK+G H G+ P FG DE +LR LE Y+PGA G L Sbjct: 252 GIYYGFPSFGGCGLKLGYHTFGQKIDPDTINREFGVYPEDESNLRAFLEEYMPGANGELK 311 Query: 293 KGSVCKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNI 352 +G+VC Y T DE FIID HP + ++++A E+L+ LA ++ +I Sbjct: 312 RGAVCMYTKTLDEHFIIDLHPEHSNVVIAAGFSGHGFKFSSGVGEVLSQLALTGKTEHDI 371 Query: 353 GPFSLSR 359 FS++R Sbjct: 372 SIFSINR 378 >P23342.1 MSOX_BACSN Monomeric sarcosine oxidase EC=1.5.3.1 Length = 387 Score = 283 bits (725), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 151/364 (41%), Positives = 205/364 (56%), Gaps = 5/364 (1%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGYYLA+QGVKTLLVD+FDPPHT GSHHG+TR+IRHAY Y+ ALRA LW Sbjct: 15 MGMAAGYYLAKQGVKTLLVDSFDPPHTNGSHHGDTRIIRHAYGEGREYVPFALRAQELWY 74 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSFADRLAEAEK-RQVRVERLDADEIRRRWPVLNLP 119 ELE+E+ ++ +GVL + +F EA + E + ++ RW + +P Sbjct: 75 ELEKETHHKIFTQTGVLVYGPKGGSAFVSETMEAANIHSLEHELFEGKQLTDRWAGVEVP 134 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 +++ A++EP++G L+SE CI YR+LA AHGA E VT+ T G Y Sbjct: 135 DNYEAIFEPNSGVLFSENCIQAYRELAEAHGATVLTYTPVEDFEVTEDLVTIKTAKGSYT 194 Query: 180 GAAAILSAGAWFGSLPPF--AALPIQAIRKVVGWFQS--TPDFDAGSFPGFTLGTGEGGY 235 ++S GAW L +P+Q R+VVG+F+ + +P F + G Y Sbjct: 195 ANKLVVSMGAWNSKLLSKLDVEIPLQPYRQVVGFFECDEAKYSNNAHYPAFMVEVENGIY 254 Query: 236 YGFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLLKGS 295 YGFPS GG+GLKIG H G+ P FG+ DE +LR+ LE Y+PGA G L KG+ Sbjct: 255 YGFPSFGGSGLKIGYHSYGQQIDPDTINREFGAYPEDEANLRKFLEQYMPGANGELKKGA 314 Query: 296 VCKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNIGPF 355 VC Y TPDE F+ID HP Y ++ +A E LA LA ++ +I F Sbjct: 315 VCMYTKTPDEHFVIDLHPKYSNVAIAAGFSGHGFKFSSVVGETLAQLATTGKTEHDISIF 374 Query: 356 SLSR 359 SL+R Sbjct: 375 SLNR 378 >Q6ITC6.2 Q6ITC6_9BACI Monomeric sarcosine oxidase EC=1.5.3.1 Length = 387 Score = 281 bits (720), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 149/364 (40%), Positives = 204/364 (56%), Gaps = 5/364 (1%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGYYLA+QGVKTLLVD+FDPPHT GSHHG+TR+IRHAY Y+ ALRA LW Sbjct: 15 MGMAAGYYLAKQGVKTLLVDSFDPPHTNGSHHGDTRIIRHAYGEGREYVPFALRAQELWY 74 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSFADRLAEAEK-RQVRVERLDADEIRRRWPVLNLP 119 ELE+E+ ++ +GVL + F EA K + E + ++ RW + +P Sbjct: 75 ELEKETHHKIFTQTGVLVFGAKGESDFVAETMEAAKIHSLEHELFEGKQLTERWAGVEVP 134 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 E++ A++EP++G L+SE CI YR+LA AHGA+ E V + T G Y Sbjct: 135 ENYEAIFEPNSGVLFSENCIQAYRELAEAHGAKVLTYAPVEDFEVSEDLVKIQTAKGLYT 194 Query: 180 GAAAILSAGAWFGSLPPF--AALPIQAIRKVVGWFQSTPDFDAGS--FPGFTLGTGEGGY 235 I+S GAW L +P+Q R+VV +F+ + + + +P F + G Y Sbjct: 195 ANKLIVSMGAWNSKLLSKLGVEIPLQPYRQVVAYFECNEEKYSNNVHYPAFMVEVANGIY 254 Query: 236 YGFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLLKGS 295 YGFPS GG+GLKIG H G+ P FG+ DE +LR+ LE Y+P A G KG+ Sbjct: 255 YGFPSFGGSGLKIGYHTYGQEIDPDTINREFGAYPEDEANLRKFLEKYMPEANGEFKKGA 314 Query: 296 VCKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNIGPF 355 VC Y TPDE F+ID HP Y ++++ E LA LA ++ +I F Sbjct: 315 VCMYTKTPDEHFVIDLHPKYSNVVIGAGFSGHGFKFSSAVGETLAQLAVTGKTEHDISIF 374 Query: 356 SLSR 359 SL+R Sbjct: 375 SLNR 378 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,363,815,393 Number of extensions: 104233034 Number of successful extensions: 219163 Number of sequences better than 1.0e-05: 375 Number of HSP's gapped: 218185 Number of HSP's successfully gapped: 375 Length of query: 376 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 239 Effective length of database: 1,770,017,148 Effective search space: 423034098372 Effective search space used: 423034098372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)