BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0026.p (229 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DF44.1 B1DF44_9BACL Phage shock protein A, PspA 77 2e-12 B3KKS9.1 B3KKS9_9BACI PspA/IM30 family protein 73 2e-11 A8FHA4.1 A8FHA4_BACP2 Bacteriophage shock protein A 66 3e-09 C1TC00.1 C1TC00_9FIRM Phage shock protein A (IM30), suppresses s... 65 5e-09 B3KKT1.1 B3KKT1_9BACI PspA/IM30 family protein 63 2e-08 >B1DF44.1 B1DF44_9BACL Phage shock protein A, PspA Length = 233 Score = 76.6 bits (187), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 69/235 (29%), Positives = 113/235 (48%), Gaps = 23/235 (9%) Query: 3 MSIFQRITTLTKAAIHEGLNKLEDPVLLTGQYLRDLEEKISTAETKQREFQAAASVLERR 62 M IF+R+ LTKA+++E L+K+EDPV++ QYLRD+E +I AE + A L++R Sbjct: 1 MGIFKRMKDLTKASVNEVLDKMEDPVVMLNQYLRDMESEIHQAEVTVAKQMANERRLKQR 60 Query: 63 IYEN-QVLAERSEAEAVQAMNEGNEPAARAAVMAKLRYTESVQECTAGLEETRETLAVLD 121 + E + +A+R E++A A+ G E AR + KL + + V E + + + L Sbjct: 61 LDEAVRSIADR-ESKAEAALRAGQEEFARKLLEEKLYFDQKVNEYSDLHAQAKTQAEELM 119 Query: 122 SSLLNAREEHXXXXXXXXXXXXXXXXXXXXXXXSESSSYSAHPGHPGQILNAGAASRGFE 181 L ++E ++ SS +++G ASRGF Sbjct: 120 QQLHGMKDEFYQLRNKRNELASRAQIAKAQKQMAQISS--------NHTIDSGTASRGFY 171 Query: 182 RMEDKINEWEAAVE------SSAHYSAGIPESPLNSA-------VEAELERLRSR 223 RME+KI + EA + S+A+ G +SA V+ +LE L+S+ Sbjct: 172 RMEEKIMQLEAEADVLRTPYSAAYSGTGNNAGSFSSADMEKKLKVDEQLEALKSK 226 >B3KKS9.1 B3KKS9_9BACI PspA/IM30 family protein Length = 228 Score = 72.8 bits (177), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 57/194 (29%), Positives = 94/194 (48%), Gaps = 16/194 (8%) Query: 3 MSIFQRITTLTKAAIHEGLNKLEDPVLLTGQYLRDLEEKISTAETKQREFQAAASVLERR 62 M +F+RI +TKA+++E L+K+EDP+++ QYLRD+E +I AE + A +++R Sbjct: 1 MGVFKRIKDMTKASVNELLDKVEDPIVMLNQYLRDMEAEIHEAEVTVAKQMANERRMKQR 60 Query: 63 IYENQVLAERSEAEAVQAMNEGNEPAARAAVMAKLRY----TESVQECTAGLEETRETLA 118 + E +A + EA+A A+ G E AR + K+ + TE V+ + +E +A Sbjct: 61 VDEAVKMAAQREAQAELALKNGQEEVARKLLEEKIYFDQKETEYVELHLQSEAQAKELVA 120 Query: 119 VLDSSLLNAREEHXXXXXXXXXXXXXXXXXXXXXXXSESSSYSAHPGHPGQILNAGAASR 178 L ++E S+ SS + +G+AS Sbjct: 121 ----QLHEMKDEFYKMRNKRNELVARAQMAKAKKQMSQVSSL--------HTIESGSASM 168 Query: 179 GFERMEDKINEWEA 192 GF RME+KI + EA Sbjct: 169 GFHRMEEKIMQMEA 182 >A8FHA4.1 A8FHA4_BACP2 Bacteriophage shock protein A Length = 221 Score = 65.9 bits (159), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 57/227 (25%), Positives = 106/227 (46%), Gaps = 30/227 (13%) Query: 5 IFQRITTLTKAAIHEGLNKLEDPVLLTGQYLRDLEEKISTAE---TKQREFQAAASVLER 61 +F+R+ + A ++EGL+K+E+P ++ QY+RD+E+ I+ A+ KQ+ Q R Sbjct: 2 VFRRMRDMFVATVNEGLDKMENPRVMLNQYVRDMEDDIAKAKHAIIKQQTIQQG---FLR 58 Query: 62 RIYENQVLAERSEAEAVQAMNEGNEPAARAAV--MAKL-----RYTESVQECTAGLEETR 114 + E + AE+ + +A A + G E AR A+ M Y E+ Q+ L+E + Sbjct: 59 KAEETEAFAEKRKKQAELAFHAGEEELARKALTEMKYFEEKHQEYQEAYQQSVKQLKELK 118 Query: 115 ETLAVLDSSLLNAREEHXXXXXXXXXXXXXXXXXXXXXXXSESSSYSAHPGHPGQILNAG 174 E + L++ L + +++ + ++ H +++ Sbjct: 119 EQMQQLETKLRDIKDK-----------------KQALIARANAAQAKQHMNESMNKVDSE 161 Query: 175 AASRGFERMEDKINEWEAAVESSAHYSAGIPESPLNSAVEAELERLR 221 +A + F RME++I E E S A ++ + LN A E E E L+ Sbjct: 162 SAYKEFLRMENRIEEMETKAGSYAQFADQGAYAHLNYADEVEKEWLK 208 >C1TC00.1 C1TC00_9FIRM Phage shock protein A (IM30), suppresses sigma54-dependent transcription Length = 224 Score = 64.7 bits (156), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 62/229 (27%), Positives = 98/229 (42%), Gaps = 18/229 (7%) Query: 3 MSIFQRITTLTKAAIHEGLNKLEDPVLLTGQYLRDLEEKISTAETKQREFQAAASVLERR 62 MS+F+RI +A I+ L+K EDPV + QYLRD+ E I+ AET + A E + Sbjct: 1 MSLFKRIGDNIRANINSLLDKAEDPVKMLEQYLRDMAEDIAEAETAVAKQIAVVKRFEAQ 60 Query: 63 IYENQVLAERSEAEAVQAMNEGNEPAARAAVMAKLRYTESVQECTAGLEETRETLAVLDS 122 E Q + E+ E++A+ A+ G E AR A+ K E A ++ R+ L Sbjct: 61 YQEAQAMVEKRESQAMAAVESGREDLARRALEDKRLNQTKADEFYAQYQQYRDVADRLKL 120 Query: 123 SLLNAREEHXXXXXXXXXXXXXXXXXXXXXXXSESSSYSAHPGHPGQILNAGAASRGFER 182 L + +EE+ ++ S G GFER Sbjct: 121 QLTDMKEEYEKMKARKAALVARAQAAKAQKSVNDVMSGFGKNNARG----------GFER 170 Query: 183 MEDKINEWEAAVESSAHY--SAGIPESPL------NSAVEAELERLRSR 223 ME+K+ + EA ++ S + L S ++ E++RLR + Sbjct: 171 MEEKVLQAEAEAQAGIELRSSGDNLDDELAALGQGTSDIDIEMQRLREK 219 >B3KKT1.1 B3KKT1_9BACI PspA/IM30 family protein Length = 225 Score = 63.2 bits (152), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 59/229 (25%), Positives = 106/229 (46%), Gaps = 19/229 (8%) Query: 3 MSIFQRITTLTKAAIHEGLNKLEDPVLLTGQYLRDLEEKISTAETKQREFQAAASVLERR 62 MS+F+RI +T A ++E L + +DPV L Q+L + I+ AE Q++ Q + L+++ Sbjct: 1 MSVFRRIRDITVATLNEKLEQSQDPVRLIDQFLHSTRQDIAEAEKLQQQCQVHSRQLKQQ 60 Query: 63 IYENQVLAERSEAEAVQAMNEGNEPAARAAVMAKLRYTESVQECTAGLEETRETLAVLDS 122 + + + + + E +A+ A+ G E A+ A+ KL Y E +++ L + E+L ++ Sbjct: 61 VDQAETMRFKREEQALLALKAGEEHLAKLALQEKLIYEEKIEQYQGLLSSSLESLKEIEE 120 Query: 123 SLLNAREEHXXXXXXXXXXXXXXXXXXXXXXXSE-SSSYSAHPGHPGQILNAGAASRGFE 181 L R E+ +E + SY GH + F Sbjct: 121 QLGELRMEYQTVYSKRQYYVARVETLRLQQKMNERTGSYGG--GHD--------VPKMFN 170 Query: 182 RMEDKINEWEAAVES-------SAHYSAGIPESPLNSAVEAELERLRSR 223 R+ED+I++WE S Y ES +++ +E EL RL+ + Sbjct: 171 RLEDRISDWELEARSLRDLRRMGQEYMDQAGES-VSTVLEKELARLKQK 218 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.308 0.122 0.321 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,029,717,384 Number of extensions: 32280129 Number of successful extensions: 105932 Number of sequences better than 1.0e-05: 141 Number of HSP's gapped: 105977 Number of HSP's successfully gapped: 141 Length of query: 229 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 98 Effective length of database: 1,826,543,238 Effective search space: 179001237324 Effective search space used: 179001237324 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 129 (54.3 bits)