BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0021.p (260 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9KFW1.1 Q9KFW1_BACHD Carbonic anhydrase EC=4.2.1.1 241 4e-62 A4WF75.1 A4WF75_ENT38 Carbonate dehydratase EC=4.2.1.1 214 5e-54 C2X5E7.1 C2X5E7_BACCE Carbonic anhydrase 213 1e-53 C2U6I2.1 C2U6I2_BACCE Carbonic anhydrase 212 3e-53 B6FIM3.1 B6FIM3_9ENTR Putative uncharacterized protein 206 1e-51 >Q9KFW1.1 Q9KFW1_BACHD Carbonic anhydrase EC=4.2.1.1 Length = 275 Score = 241 bits (615), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 118/227 (51%), Positives = 148/227 (65%), Gaps = 2/227 (0%) Query: 30 AHQAHWSYEGETSPEHWAELDELFTTCSTGKAQSPINILQEKVKDEGSLXXXXXXXXXXX 89 AH+ HWSY G+T PEHWAELD + C+ G+ QSPIN+ + + D + Sbjct: 45 AHEVHWSYTGDTGPEHWAELDSEYGACAQGEEQSPINLDKAEAVDTDT--EIQVHYEPSA 102 Query: 90 XXXINNGHTIQVNLKNQKNRITVEGKTYTLQQFHFHLPSEHEVDGKHADMELHFVHKNEE 149 NNGHTIQ + N I ++GK YTL QFHFH+PSEHE++GK+ DMELHFVHKNE Sbjct: 103 FTIKNNGHTIQAETTSDGNTIEIDGKEYTLVQFHFHIPSEHEMEGKNLDMELHFVHKNEN 162 Query: 150 GQLAVLSVLITKGTENAGLNKLWSVLPGEESEEEVPVNGDFDMNKLLPADLHSFRYQGSL 209 +LAVL VL+ G EN L KLWS LP EE+EE + ++ D+N LLP F Y GSL Sbjct: 163 DELAVLGVLMKAGEENEELAKLWSKLPAEETEENISLDESIDLNALLPESKEGFHYNGSL 222 Query: 210 TTPPCTEGVQWIVLEHPVQWSGEQINQFAAIFPHDNRPVQALGSREV 256 TTPPC+EGV+W VL P+ S EQI+ FA IFP ++RPVQ R+V Sbjct: 223 TTPPCSEGVKWTVLSEPITVSQEQIDAFAEIFPDNHRPVQPWNDRDV 269 >A4WF75.1 A4WF75_ENT38 Carbonate dehydratase EC=4.2.1.1 Length = 246 Score = 214 bits (545), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 111/243 (45%), Positives = 151/243 (62%), Gaps = 6/243 (2%) Query: 16 GKAEGTQEPPAKASAHQAHWSYEGETSPEHWAELDELFTTCSTGKAQSPINILQEKVKDE 75 GKA ASA+ +HWSYEGE SPEHW LDE + TC +G QSP+NI +K Sbjct: 6 GKAAILALSIVSASAYASHWSYEGEGSPEHWGALDEAYKTCQSGMNQSPVNI-DSTIK-- 62 Query: 76 GSLXXXXXXXXXXXXXXINNGHTIQVNLK-NQKNRITVEGKTYTLQQFHFHLPSEHEVDG 134 L NNGHTIQ K + + IT++ +++ LQQFHFH PSE+ + G Sbjct: 63 AHLTPLKTHYIDGPVTLTNNGHTIQAAEKPDTPDTITLDNQSWALQQFHFHAPSENTIHG 122 Query: 135 KHADMELHFVHKNEEGQLAVLSVLITKGTENAGLNKLWSVLPGEESEEEVPVNGDFDMNK 194 K DME+H VHKN +G L V++V+ KG NA L+KLW ++PG ++E+ ++ D+NK Sbjct: 123 KKFDMEMHLVHKNADGALTVVAVMFEKGAANAELDKLWGIMPG-QAEQSATLDTKLDLNK 181 Query: 195 LLPADLHSFRYQGSLTTPPCTEGVQWIVLEHPVQWSGEQINQFAAIFPHD-NRPVQALGS 253 LLP + +R+ GSLTTPPC+EGV W+VL++P+ S Q+ +F HD NRPVQ+L Sbjct: 182 LLPKNKTYWRFSGSLTTPPCSEGVTWVVLKNPMTLSATQLEKFTHTMHHDNNRPVQSLHG 241 Query: 254 REV 256 R V Sbjct: 242 RVV 244 >C2X5E7.1 C2X5E7_BACCE Carbonic anhydrase Length = 243 Score = 213 bits (542), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 106/242 (43%), Positives = 144/242 (59%), Gaps = 2/242 (0%) Query: 16 GKAEGTQEPPAKASAHQ-AHWSYEGETSPEHWAELDELFTTCSTGKAQSPINILQEKVKD 74 G QE P K S + WSYEG T PEHW EL + C G+ QSPI+I E++K Sbjct: 2 GCNTAKQEAPHKQSTKENTQWSYEGTTGPEHWGELKPEYKICLNGQEQSPIDIKTEQIKS 61 Query: 75 EGSLXXXXXXXXXXXXXXINNGHTIQVNLKNQKNRITVEGKTYTLQQFHFHLPSEHEVDG 134 NNGH+I+ + N +T+ YTL+QFHFH PSEH+ +G Sbjct: 62 TVDNNLLQINYQPISFSIKNNGHSIEGKANSSDNYLTLGENRYTLKQFHFHTPSEHQFEG 121 Query: 135 KHADMELHFVHKNEEGQLAVLSVLITKGTENAGLNKLWSVLPGEESEEEVPVNGDFDMNK 194 KHADMELH VH+N++GQL V+ ++I +G +N G +W LP ++ + V D+ + Sbjct: 122 KHADMELHLVHQNDQGQLVVVGIMIKEGQKNEGFAAMWQNLPHRKN-IKADVQHTIDIKQ 180 Query: 195 LLPADLHSFRYQGSLTTPPCTEGVQWIVLEHPVQWSGEQINQFAAIFPHDNRPVQALGSR 254 +LP+D SFRY GSLTTPPCTE VQWIV++ ++ S +QI F +FP +NRPVQ + R Sbjct: 181 ILPSDYSSFRYMGSLTTPPCTENVQWIVMKQTIEMSKKQIKVFHKLFPTNNRPVQPINGR 240 Query: 255 EV 256 V Sbjct: 241 AV 242 >C2U6I2.1 C2U6I2_BACCE Carbonic anhydrase Length = 263 Score = 212 bits (539), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 107/253 (42%), Positives = 148/253 (58%), Gaps = 10/253 (3%) Query: 5 VMAGCGGNNQNGKAEGTQEPPAKASAHQ-AHWSYEGETSPEHWAELDELFTTCSTGKAQS 63 V+ GC QE P K S + WSY+G T PEHW EL + C G+ QS Sbjct: 19 VLMGCN--------TAKQEAPQKQSTKENTQWSYKGTTGPEHWGELKPEYNMCLNGQEQS 70 Query: 64 PINILQEKVKDEGSLXXXXXXXXXXXXXXINNGHTIQVNLKNQKNRITVEGKTYTLQQFH 123 PI+I E++K NNGH+I+ + + +T+ YTL+QFH Sbjct: 71 PIDIKTEQIKSTVDNNLLQINYQPISFSIKNNGHSIEGKANSSDDYLTLGENRYTLKQFH 130 Query: 124 FHLPSEHEVDGKHADMELHFVHKNEEGQLAVLSVLITKGTENAGLNKLWSVLPGEESEEE 183 FH PSEH+ +GKHADMELH VH+N++GQLAV+ ++I +G +N G +W LP ++ + Sbjct: 131 FHTPSEHQFEGKHADMELHLVHQNDQGQLAVVGIMIKEGQKNEGFAAMWQNLPHRKN-IK 189 Query: 184 VPVNGDFDMNKLLPADLHSFRYQGSLTTPPCTEGVQWIVLEHPVQWSGEQINQFAAIFPH 243 V D+ ++LP+D SFRY GSLTTPPCTE VQWIV++ ++ S +QI F +FP Sbjct: 190 ADVQHTIDIKQILPSDHSSFRYMGSLTTPPCTENVQWIVMKQTIEMSKKQIKVFHKLFPT 249 Query: 244 DNRPVQALGSREV 256 +NRPVQ + R V Sbjct: 250 NNRPVQPINGRAV 262 >B6FIM3.1 B6FIM3_9ENTR Putative uncharacterized protein Length = 246 Score = 206 bits (525), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 114/243 (46%), Positives = 152/243 (62%), Gaps = 6/243 (2%) Query: 16 GKAEGTQEPPAKASAHQAHWSYEGETSPEHWAELDELFTTCSTGKAQSPINILQEKVKDE 75 GKA ASA+ +HWSYEGE +PEHW ELDE + TC +G QSPINI + Sbjct: 6 GKAALLALSIISASAYASHWSYEGEGAPEHWGELDEAYKTCQSGMNQSPINI---DSTLK 62 Query: 76 GSLXXXXXXXXXXXXXXINNGHTIQVNLK-NQKNRITVEGKTYTLQQFHFHLPSEHEVDG 134 L NNGHTIQ K + ++ IT++ +T++LQQFHFH PSE+ + G Sbjct: 63 AHLTPLQTHYTEGPITLTNNGHTIQAGEKADTRDSITLDKQTWSLQQFHFHAPSENTIHG 122 Query: 135 KHADMELHFVHKNEEGQLAVLSVLITKGTENAGLNKLWSVLPGEESEEEVPVNGDFDMNK 194 K+ ME+H VHKN EG+L V++V+ KG NA L KLW V+P E +E+ +N + ++N Sbjct: 123 KNYAMEMHLVHKNAEGELVVVAVMFDKGAPNAELEKLWKVIP-EHAEQNATINQNLNLNN 181 Query: 195 LLPADLHSFRYQGSLTTPPCTEGVQWIVLEHPVQWSGEQINQFAAIFPHD-NRPVQALGS 253 LLP D +R+ GSLTTPPC+EGV W+VL+HP+ S EQ+ +F HD NR VQ+L Sbjct: 182 LLPKDKTYWRFSGSLTTPPCSEGVIWVVLKHPLTLSAEQLEKFTHTMHHDNNRSVQSLHG 241 Query: 254 REV 256 R V Sbjct: 242 RVV 244 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.130 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,632,833,934 Number of extensions: 67723413 Number of successful extensions: 117999 Number of sequences better than 1.0e-05: 880 Number of HSP's gapped: 116329 Number of HSP's successfully gapped: 884 Length of query: 260 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 127 Effective length of database: 1,807,701,208 Effective search space: 229578053416 Effective search space used: 229578053416 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 130 (54.7 bits)