BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0020.p (1266 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9G8.1 B3K9G8_9BACI Metallophosphoesterase 241 3e-61 Q2RJB5.1 Q2RJB5_MOOTA Metallophosphoesterase 207 8e-51 C1I4R8.1 C1I4R8_9CLOT Metallophosphoesterase 186 1e-44 C1T8N3.1 C1T8N3_9FIRM Predicted phosphohydrolase 186 2e-44 B1DD75.1 B1DD75_9BACL PA14 domain protein 182 3e-43 >B3K9G8.1 B3K9G8_9BACI Metallophosphoesterase Length = 2050 Score = 241 bits (616), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 150/463 (32%), Positives = 228/463 (49%), Gaps = 14/463 (3%) Query: 506 DGVTRGEYTAIRLPDGKTTGWIPTAAVDVKLLKDGWGLTDAQGEIRXXXXXXXXXXXXXQ 565 DG+ G T ++ D + P V V G TDA G ++ Q Sbjct: 1010 DGIVEGYETEFKVTDAQGQ---PVEGVQVMADAAAVGTTDAAGILKTGALTSQVKTYTVQ 1066 Query: 566 AVNGDKVSKVFSLSVVPQLGSDMPEYVRIYVTEDMKTTQSIVWKTAPRVERGVIQYVEAS 625 AV ++ S V P GS P + + + ED T++ + W T P + V++ + S Sbjct: 1067 AVKNNQFSPALEFKVSPLAGSPTPFNISVGMGEDPATSRMLNWHTDPATDGTVVELAKQS 1126 Query: 626 KFTGFDQPNIKEQTAEPQLLLAPDRVGETLFHKGMLQSLSPGTEYIYRVG-SPGLWSEQH 684 +FT FD PN+++ +L D +G HK L PGTEY+YRVG G +S Q Sbjct: 1127 EFTDFDAPNVQKFDGASELYHTLD-LGTVRVHKATAAGLEPGTEYVYRVGDGQGHYSAQG 1185 Query: 685 RFTTESANPEAFSFLFVTDSHTNTEEAYHIHQNLLSSAFTSYPDARFVMHGGDIVDDGGI 744 F T + FL+ DS +T + + + N + A +PDA F++H GD+VD G + Sbjct: 1186 SFKTTELAGDTTKFLYFADSQASTAKEFELWGNTIDKAAAEHPDAEFMIHAGDMVDKGFL 1245 Query: 745 MNEWEQDMKASQLYSGSVPSAYTMGNHDVKNGGKEV--FRTALGLPGNGMPGQEQLTYSY 802 +W +Q + + +GNH+V G KE F PGNG+ + +S+ Sbjct: 1246 EEQWNYWFDEAQKHFLNTTLVSAIGNHEVM-GNKENGDFLAHFNQPGNGLDSLKGTNFSF 1304 Query: 803 DYEDTHFIVLNSEADEVDMQKQAEWLRGNLQASSKKWNIAMFHRPAYHTEDGRGPELVTK 862 DY+D HFI+LNS E ++ Q +WL+ +L ++K+W IAMFHR +Y + V Sbjct: 1305 DYKDVHFIMLNS---EYQLEDQKKWLQQDLANNTKEWTIAMFHRGSYGSIYDSAE--VRS 1359 Query: 863 YLAPVLEELGADLVLVGHDHALAWTYPMKNGQPIKDGSPGTVYLSGGSSGWKFYDAVKFD 922 APVLEE G DLVL GHDH +YPM N Q DG GT Y+ GSSG KFY + Sbjct: 1360 LWAPVLEEFGVDLVLNGHDHIYIRSYPMMNNQIAADGK-GTTYVVAGSSGPKFYSHTERG 1418 Query: 923 YLEYLYDDNFPVYSAITIGKDQILIEARKADGEQLQALTIEKA 965 + E + ++ +Y+++ + D++ + G + T+ K+ Sbjct: 1419 WHEVVDEEKTQMYASVEVKGDELHFVTKTVGGRVVDEFTLTKS 1461 Score = 184 bits (467), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 134/458 (29%), Positives = 220/458 (48%), Gaps = 35/458 (7%) Query: 29 VIIEDFENNLSDNVLIQKRNFSGAM------SLESDPRHVRSGNNSVRVDYDYIGVKENP 82 V++EDFEN LS + + +GA+ S+E+D ++RSGN +++++YD+ G+ Sbjct: 591 VMLEDFENGLS-----RYKPSAGALFKYSRVSIETDDTYIRSGNGALKLEYDFTGIT-GT 644 Query: 83 SYVYV---GPAAPYPVNGLPQKIGMWVYGNNDGHLIFSKFRDGNGKSFEVEYLDENVGVD 139 S Y+ G A + G P+KI MWVYG+ H + ++ RD G + + + +E GVD Sbjct: 645 SGAYLQTNGTADNIEIPGYPEKISMWVYGDGKAHWLRAQLRDSKG-TIALNFTEETTGVD 703 Query: 140 WTGWKYIEGDIPQDKVGPITLEIYMRIEQSIFDKKNKGTVWVDDVRLIYEQDPNEDMAVP 199 + GWKY+E +P+ + P+ +++ +R ++ KK+ G ++VD++R +Y N+D+ P Sbjct: 704 FKGWKYLEASVPKGRTLPLVMDMPVRYMETKDAKKDAGAIYVDEIRALY-GPANDDIDPP 762 Query: 200 TLDVLSPANEAVIDTNSPRIQLAAADA---------QSGIDPGSVTLNLDGHPVQPEYEA 250 L SPA+ A I N+P I + A DA + IDP + LDG VQ Sbjct: 763 VLKKFSPADGAEIKENTPTITVYAEDAGYDPVAHPGTTLIDPDKIRFYLDGSLVQHTLYP 822 Query: 251 ASGLISYQPSAPLAGGYHTVQSAVYDRSGNPAESSSTFFIQ-GGIQYHLDAPTEVISNKT 309 G I Y PS PLA G H V+ V D SGN F + G + D P + + Sbjct: 823 PEGRIHYTPSVPLADGVHQVKVTVRDLSGNQTTKQWNFTVNTGSAKVVYDTPETIYVGGS 882 Query: 310 FQLKLQVKNAGDLQDSYAQLQYDPQTLEITTAEP--QVAAPSVAQNEIDPVKGQYRLQLA 367 + L + ++ + Q+DP +E P +++A V+ + ID G+ R+ Sbjct: 883 YTLDIGGVKTSQIRSGHVDFQFDPTKVENLELIPGDKLSASQVSTS-IDGTTGKVRVTWK 941 Query: 368 GLSKDSLPADETIALLNVAVKPSAKMERGEAFKSIVMSDGSFRMTVGE--AVYALAAPHK 425 + SL + I + VK +A G + ++ SF T + Y L K Sbjct: 942 DIHTASLQDTDWIGQIRYDVKKNAT---GSSIIALTSGAVSFTDTGNTEFSFYGLPIESK 998 Query: 426 YTIGFPYKLTVKGSSLHTKSTLRVTDDAGVPAEGVSII 463 T + G ++ +VTD G P EGV ++ Sbjct: 999 ITTHYALSWNEDGIVEGYETEFKVTDAQGQPVEGVQVM 1036 Score = 63.5 bits (153), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 42/171 (24%), Positives = 70/171 (40%), Gaps = 5/171 (2%) Query: 1086 ADLWSTRTISAFLKLKVINGYADGTFRPDKTITRGEFAAVVAKAFGLAKKSDASQLSDVN 1145 + W+ I ++I G + F P + +TR EFAA++ + L + A + Sbjct: 1876 SSFWAAEVIGDLAAKQLIEGVSADRFAPAQQVTRAEFAAMLVRLLDLKAEGPAPFADVAS 1935 Query: 1146 THWAQEAIAVLASNGIIAGYPDGTFKPXXXXXXXXXXXXXXXXVNF---NNAAPVKAGIT 1202 W EA++ A +GI+ G F P + AG Sbjct: 1936 GKWYAEAVSAAAQSGIVNGTAKDRFAPDAPIKRQEMAAMLVRAYAHAMKQQQENISAGSG 1995 Query: 1203 FSDTANS--WNKDQIARAAQIGLINGKGNGKFAPAQSSTRAEALTIVMNAL 1251 F+D A S W ++ + A +G + G +FAP +TRAE+ ++ N L Sbjct: 1996 FADLAESPDWVREAVETAHALGFVQGHAPSRFAPEGLTTRAESAQVIHNLL 2046 >Q2RJB5.1 Q2RJB5_MOOTA Metallophosphoesterase Length = 560 Score = 207 bits (526), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 126/397 (31%), Positives = 201/397 (50%), Gaps = 19/397 (4%) Query: 585 GSDMPEYVRIYVTEDMKTTQSIVWKTAPRVERGVIQYVEASKFTGFDQPNIKEQTAEPQL 644 S P+++ + T+D TTQ+I W+T + RG++QY +A+ F K T E + Sbjct: 36 ASAQPDHITLTWTQDPLTTQTITWRTNITIARGLVQYAKAADKASFPG---KAATVEATV 92 Query: 645 LLAPDRVGETLFHKGMLQSLSPGTEYIYRVGSPGLWSEQHRFTTESANPEAFSFLFVTDS 704 +G+ H L L PGTEYIYRVG WS+ H FTTE++N +F FL DS Sbjct: 93 QKFTSDLGDMNIHTATLTGLEPGTEYIYRVGDGTNWSDIHTFTTEASNTHSFKFLIFGDS 152 Query: 705 HTN--TEEAYHIHQNLLSSAFTSYPDARFVMHGGDIVDDGGIMNEWEQDMKASQLYSGSV 762 + Y + + +AF + DA+F ++ GD+V+ G W + +A++ ++ Sbjct: 153 QSGDPLNPEYKPWHDTIQNAFKTNTDAKFFVNVGDLVEQGQNYVHWNKWFEAAKGVIDTI 212 Query: 763 PSAYTMGNHDVKN-----GGKEVF-RTALGLPGNGMPGQEQLTYSYDYEDTHFIVLNSEA 816 P+ T GNH+ N K +F T LP NG G + YS+DY + H ++L+S+ Sbjct: 213 PAMATQGNHETYNPPDGHSTKPIFWTTQFKLPQNGPEGLKGQAYSFDYGNAHIVMLDSQE 272 Query: 817 DEVD------MQKQAEWLRGNLQASSKKWNIAMFHRPAYHTEDGRGPELVTKYLAPVLEE 870 +E + Q WL +LQ ++K W + FH+ Y+ + R E + P+ ++ Sbjct: 273 EEEKGVAGDILAAQKAWLEKDLQNTNKPWKLVFFHKTPYYNKATRTNEDIKAAFQPLFDK 332 Query: 871 LGADLVLVGHDHALAWTYPMKNGQPIKDGSPGTVYLSGGSSGWKFY-DAVKFDYLEYLYD 929 D+V GHDHA+A TYP+ + + + GT+Y G SG K+Y D + + YD Sbjct: 333 YHVDVVFNGHDHAVARTYPIAGDKFVSSPAKGTIYYLTGRSGNKYYPDLSAKVWDAFFYD 392 Query: 930 -DNFPVYSAITIGKDQILIEARKADGEQLQALTIEKA 965 + P Y + D++ + A K DG + TI+KA Sbjct: 393 PQDQPNYIVAELNGDKLTLRAMKQDGTPIDTYTIDKA 429 >C1I4R8.1 C1I4R8_9CLOT Metallophosphoesterase Length = 600 Score = 186 bits (472), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 124/399 (31%), Positives = 197/399 (49%), Gaps = 26/399 (6%) Query: 584 LGSDMPEYVRIYVTEDMKTTQSIVWKTAPRV--ERGVIQYVEASKFT--GFDQPNIKEQT 639 L +P + TED KT +SI W + P ++ V+QY+ SK+ G + I + Sbjct: 54 LKDKIPSNILFGTTEDAKTQKSISWMSNPLTSKDKVVVQYIMKSKYNSKGLLRNKINTKE 113 Query: 640 AEPQLLLAPDRVGETLFHKGMLQS-------LSPGTEYIYRVGSPGLWSEQHRFTTESAN 692 E + + G+++S L PGT Y+ RVG WSE FTT + Sbjct: 114 VEGEYTNLTFSGNSDINLNGVVRSNQIKITGLEPGTTYMCRVGDGENWSEFMEFTT-LED 172 Query: 693 PEAFSFLFVTDSHTNTEEAYHIHQNLLSSAFTSYPDARFVMHGGDIVDDGGIMNEWE--Q 750 E F+ + D+ + +E+ +L+ + D+ ++H GD +DD + N+W Sbjct: 173 KENMKFIILGDTQSPSEQGLSELDKILNIIENEHSDSDAIIHLGDFIDDSSLFNQWNFIT 232 Query: 751 DMKASQLYSGSVPSAYTMGNHDV-----KNGGKEVFRTALGLPGNGMPGQEQLTYSYDYE 805 + + S+ +GNH+ + K++F T P NG+ YS DY Sbjct: 233 SLFGKYNRANSIDMINVLGNHEYMGDPSADKAKKIFNT----PTNGIKENIGGVYSVDYN 288 Query: 806 DTHFIVLNSEADEVDMQKQAEWLRGNLQASSKKWNIAMFHRPAYHTEDGRGPELVTKYLA 865 D HF V++ +D+ ++K+ EWL+ ++ S KK I + H+P Y+T G EL+ + L Sbjct: 289 DIHFSVISFTSDKELLKKELEWLKEDINNSDKKLKILLTHQPPYYTNPDGGNELIKEMLP 348 Query: 866 PVLEELGADLVLVGHDHALAWTYPMKNGQPIKDGSPGTVYLSGGSSGWKFYDAVKFDYLE 925 PV EELG DLV GHDHA T +KNG I+D S GT+Y+ GG++G K Y AV E Sbjct: 349 PVAEELGIDLVFSGHDHAYGRTKRLKNG--IEDKS-GTIYIVGGTTGPKHYQAVNDGSFE 405 Query: 926 YLYDDNFPVYSAITIGKDQILIEARKADGEQLQALTIEK 964 D+N +Y+ + K I + ++K DG + ++K Sbjct: 406 VYNDENTAIYTTLEFNKGDIRVISKKPDGTIVDDFNLDK 444 >C1T8N3.1 C1T8N3_9FIRM Predicted phosphohydrolase Length = 578 Score = 186 bits (471), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 138/409 (33%), Positives = 198/409 (48%), Gaps = 21/409 (5%) Query: 566 AVNGDKVSKVFSLSVVPQLGSDMPEYVRIYVTEDMKTTQSIVWKTAPRVERGVIQYVEAS 625 A+N D++ +V QL S P+ + + T D +TTQ++ W+ V IQY+ S Sbjct: 57 ALNVDEIPAQADQNVDSQLYSTRPDQIILTWTADPQTTQTVSWRVY-GVAGSKIQYLPQS 115 Query: 626 KFTGFDQPNIKEQTAEPQLLLAPDRVGETLFHKGMLQSLSPGTEYIYRVGSPGLWSEQHR 685 T ++ A QL G L +L PGT Y+YRVG WSE Sbjct: 116 GSTDDFSSAQEKDIAGSQLY------GGFNHFVAELDNLQPGTTYVYRVGKDSGWSEPAT 169 Query: 686 FTTESANPEAFSFLFVTDSHTNT-EEAYHIHQNLLSSAFTSYPDARFVMHGGDIVDDGGI 744 FTT +A SF+F+ D H+ + + I Q LL+ A ++YPD +F + GDIVD+ Sbjct: 170 FTTAAATNNF-SFMFMGDVHSGYYDTSAGIWQQLLAQACSAYPDIKFALQAGDIVDNSED 228 Query: 745 MNEWEQDMKASQLYSGSVPSAYTMGNHDVKNGGKEVFRTALGLPGNGMPGQEQLTYSYDY 804 +W Q A+ +P GNHD +G ++F LP NG G E YS+DY Sbjct: 229 TGDWSQLFTAAAGVFDHIPLMPAEGNHD--SGDADLFNKYFALPQNGPVGYEGHNYSFDY 286 Query: 805 EDTHFIVLNSEADEVD---MQKQAEWLRGNLQASSKKWNIAMFHRPAYHTE----DGRGP 857 + HF+VL+S D Q WL +LQ S+KKW MFH PAY D Sbjct: 287 GNAHFVVLDSSLMGCDGDAYQAGITWLENDLQQSNKKWKFVMFHVPAYTINIGDNDAAAS 346 Query: 858 ELVTKYLAPVLEELGADLVLVGHDHALAWTYPMKNGQPIKDGSPGTVYLSGGSSGWKFY- 916 +++ +Y PVLE G D+V VGH H TYP+ GQ ++D G + G++G KFY Sbjct: 347 DIIRQYWVPVLERNGVDMVFVGHQHMYMRTYPIYQGQ-VQDRPTGGITYLMGNAGNKFYI 405 Query: 917 DAVKFDYLEYLYDDNFPVYSAITIGKDQILIEARKADGEQLQALTIEKA 965 + + DY+ + N Y+ I I D + + R DG L ++K Sbjct: 406 NPKQHDYVAKVL-SNVTCYTYIKIEGDVLTMITRDVDGNVLDEYKVDKG 453 >B1DD75.1 B1DD75_9BACL PA14 domain protein Length = 1174 Score = 182 bits (461), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 121/388 (31%), Positives = 191/388 (49%), Gaps = 54/388 (13%) Query: 574 KVFSLSVVPQLGSDMPEYVRIYVTEDM----KTTQSIVWKT-------AP-RVERGVIQY 621 K F+ S P S++ +Y I + + KT +S W T AP ++ +++ Sbjct: 459 KAFAFS--PSNFSEVVDYSPIAIAMSLYGSAKTNRSFAWYTPYDRPQSAPTNIKDSIVEI 516 Query: 622 VEASKFTGFDQPNIKEQTAEPQ-------LLLAPDRVGETLFHKGMLQSLSPGTEYIYRV 674 V A++ GFD PN T +P+ L + G + HK +++ L+PGT Y YR+ Sbjct: 517 VPAAE--GFDSPNKIRVTGKPEDTRVLTNLKITSSTTGSFISHKVLVEGLTPGTAYKYRL 574 Query: 675 GSPGLWSEQHRFTTESANPEAFSFLFVTDSHTNTEEAYHIHQNLLSSAFTSYPDARFVMH 734 G+ G WSE FTTES N F+FL++TDS + + Y + N + A T +PDA+F++ Sbjct: 575 GADGYWSETGSFTTESDNENNFNFLYLTDSQGSNSQDYDVWGNTMQQAITKFPDAKFLLM 634 Query: 735 GGDIVDDGGIMNEWEQDMKASQLYSGSVPSAYTMGNHDVKNGGKEVFRTALGLPGNGM-- 792 GD VD G + ++W K Q ++P +GNH+ + P + + Sbjct: 635 TGDQVDAGALESQWLDYFKKPQSILMNLPLMAAVGNHEGPYNDNYYYH--FNYPNDSIKN 692 Query: 793 ---PGQEQLTYSYDYEDTHFIVLNS------EADEVDMQKQAEWLRGNLQASSKKWNIAM 843 PG Y+YDY D H +VLN+ +A + +++ EWL+ + A+ KKW I Sbjct: 693 PLPPGS---VYAYDYGDAHIMVLNTMDIGWDDAQKESFEQEIEWLKHEVAATDKKWKIVA 749 Query: 844 FHRPAY----HTEDGRGPELVTKYLAPVLEELGADLVLVGHDHALAWTYPMKNGQPIKD- 898 FH+ Y H D L K + P+ +ELG D+VL GHDH +Y M + +P+++ Sbjct: 750 FHKAIYSVGNHALDSDILALRQK-MYPIFDELGIDVVLQGHDHTFMRSYQMYDNKPVQNV 808 Query: 899 ---------GSPGTVYLSGGSSGWKFYD 917 GT+Y+ S+ KFYD Sbjct: 809 ETDAQGRALNPDGTLYMINNSAATKFYD 836 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 7,291,143,084 Number of extensions: 322106741 Number of successful extensions: 562332 Number of sequences better than 1.0e-05: 1384 Number of HSP's gapped: 559556 Number of HSP's successfully gapped: 1668 Length of query: 1266 Length of database: 3,060,696,203 Length adjustment: 148 Effective length of query: 1118 Effective length of database: 1,666,385,983 Effective search space: 1863019528994 Effective search space used: 1863019528994 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 137 (57.4 bits)