BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0016.p (375 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAA0.1 B3KAA0_9BACI Glycosyl hydrolase family 88 509 e-142 B1DBI4.1 B1DBI4_9BACL Glycosyl hydrolase family 88 462 e-128 B1D7I5.1 B1D7I5_9BACL Glycosyl hydrolase family 88 348 3e-94 B3KCR7.1 B3KCR7_9BACI Glycosyl hydrolase family 88 316 3e-84 Q5WCV2.1 Q5WCV2_BACSK Unsaturated glucuronyl hydrolase 298 4e-79 >B3KAA0.1 B3KAA0_9BACI Glycosyl hydrolase family 88 Length = 378 Score = 509 bits (1312), Expect = e-142, Method: Compositional matrix adjust. Identities = 245/370 (66%), Positives = 282/370 (76%) Query: 6 WVEEAWSKALEKTGINSQSIGAEFPHASQGGKYVLEAPDWWTAGFWPGMLWQFYGECGDE 65 W+ EAW K+L KT N + IGA FPHASQ G Y LE P WWTAGFWPGMLW Y + G Sbjct: 9 WLTEAWEKSLVKTQRNVRRIGAGFPHASQNGVYQLEPPHWWTAGFWPGMLWLLYNDSGVA 68 Query: 66 SLKAIAEHCEKRLDEVLDGYVRLDHDLGFMWTLTSVANYKLTGNEISRIRALKAANYLSA 125 L+ AE CE LD+VL+ YVRLDHDLGFMWTLTSVA+YKLTG+E S IRALKAANYL+A Sbjct: 69 ELRTTAEQCELILDDVLNEYVRLDHDLGFMWTLTSVASYKLTGSEESMIRALKAANYLAA 128 Query: 126 RFNLKGRYIRAWNPWREGEDNSGVAIIDCCMNTSLLFWASAETGDPRYRHIAEAHMDTVL 185 RFNLKG+YIRAWNPW EGE+N+G+AIIDC MNT LLF AS +GDPRYRHIAEAHMDTV+ Sbjct: 129 RFNLKGQYIRAWNPWSEGEENAGLAIIDCAMNTPLLFLASQISGDPRYRHIAEAHMDTVV 188 Query: 186 EYFIRADGSVYHIVNFNPDTGEVKEKLGGQGYGPESAWSRGAAWAIYGLTLAYHHSGKQE 245 F+R DGSVYHIV ++P TGE E +GGQG+ PESAWSRG AWAIYGLTLAYHH+GK Sbjct: 189 RDFVRPDGSVYHIVRYDPVTGERLEGIGGQGFAPESAWSRGTAWAIYGLTLAYHHTGKSI 248 Query: 246 YLHAAQRVADFFLTRLPEDHVPHWDFRAPGEIGEIRDTXXXXXXXXXXXXXXXXXXDPES 305 +L A+ VA FFL LPEDHVP WDFRAP E RD+ ++ Sbjct: 249 FLDTAKTVAHFFLANLPEDHVPFWDFRAPDEARIYRDSSAGACAASGLLLLADKVEPEQA 308 Query: 306 HMYRSGALKIMESLFRNYGTWDQPDEEGLLLHGTSNYPENRNIDVPLIYGDFFYVEALAR 365 +YR A++I+ESL+RNYG WD EEGL+LHGTS+YPE +NIDVPLIYGDFF+VE LAR Sbjct: 309 AVYRKSAVRILESLYRNYGMWDNDKEEGLILHGTSHYPEGKNIDVPLIYGDFFFVEGLAR 368 Query: 366 LKEAGPFYWE 375 L+E GPFYWE Sbjct: 369 LREKGPFYWE 378 >B1DBI4.1 B1DBI4_9BACL Glycosyl hydrolase family 88 Length = 386 Score = 462 bits (1190), Expect = e-128, Method: Compositional matrix adjust. Identities = 232/377 (61%), Positives = 276/377 (73%), Gaps = 2/377 (0%) Query: 1 MEDTAWVEEAWSKALEKTGINSQSIGAEFPHASQGGKYVLEAPDWWTAGFWPGMLWQFY- 59 +E +WV EAW + ++K S IGAEFPHASQGGKYVLEAP WWTAGFWPG+LW + Sbjct: 10 LEIASWVNEAWEQTVQKVLRTSVRIGAEFPHASQGGKYVLEAPHWWTAGFWPGLLWLLHE 69 Query: 60 GEC-GDESLKAIAEHCEKRLDEVLDGYVRLDHDLGFMWTLTSVANYKLTGNEISRIRALK 118 GE D + IAE CE +LDEVLDGY RLDHD+GFMW LTSV N+KL G E SR RALK Sbjct: 70 GEANADLPFRRIAEECELKLDEVLDGYDRLDHDIGFMWILTSVINFKLNGGEESRRRALK 129 Query: 119 AANYLSARFNLKGRYIRAWNPWREGEDNSGVAIIDCCMNTSLLFWASAETGDPRYRHIAE 178 AANYL RFNLKG +IRAWNPW EGEDNSG AIIDC MN LL WA+ ETGDPRY H+AE Sbjct: 130 AANYLMGRFNLKGNFIRAWNPWSEGEDNSGWAIIDCAMNVPLLQWATRETGDPRYSHVAE 189 Query: 179 AHMDTVLEYFIRADGSVYHIVNFNPDTGEVKEKLGGQGYGPESAWSRGAAWAIYGLTLAY 238 AHM TVL +FIR DGSV HIV F+ TGEV E +GGQGYGPESAWSRG AWA+YGLTLA Sbjct: 190 AHMGTVLNHFIRTDGSVRHIVRFDSQTGEVAEFIGGQGYGPESAWSRGTAWAVYGLTLAA 249 Query: 239 HHSGKQEYLHAAQRVADFFLTRLPEDHVPHWDFRAPGEIGEIRDTXXXXXXXXXXXXXXX 298 H+G+++ L AA++V+ FFL++LP D VP WDFRAP E +RD+ Sbjct: 250 KHTGRKDLLDAAKQVSHFFLSQLPADSVPVWDFRAPEETKWLRDSSAGACAACGLLLLAE 309 Query: 299 XXXDPESHMYRSGALKIMESLFRNYGTWDQPDEEGLLLHGTSNYPENRNIDVPLIYGDFF 358 E+ YR +I++SL+ NYG++ + +EEGL+LHGTS+YPE +N+DVPLIYGD+F Sbjct: 310 LVPSAEASGYRGAGERILKSLYENYGSFGEEEEEGLILHGTSHYPERKNMDVPLIYGDYF 369 Query: 359 YVEALARLKEAGPFYWE 375 +VE LARLK WE Sbjct: 370 FVEGLARLKGGVAIPWE 386 >B1D7I5.1 B1D7I5_9BACL Glycosyl hydrolase family 88 Length = 376 Score = 348 bits (894), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 178/359 (49%), Positives = 231/359 (64%), Gaps = 7/359 (1%) Query: 11 WSKALEKTGINSQSIGAEFPHASQG-GKYVLEAPDWWTAGFWPGMLWQFYGECGDESLKA 69 W EK G IG + PH ++ G Y + DWWT+GFWPG+LW GDE KA Sbjct: 16 WKNLEEKVGRMIVQIGEKSPHVAKADGIYDDQRIDWWTSGFWPGILWLMNEATGDEKYKA 75 Query: 70 IAEHCEKRLDEVLDGYVRLDHDLGFMWTLTSVANYKLTGNEISRIRALKAANYLSARFNL 129 A + ++RL+ L DHD+GF + T+VA Y++TG++ ++ R L AAN+++ RFN Sbjct: 76 AAWNWDERLEAQLLQPNSFDHDVGFHFLPTAVAKYRITGDKDAQRRGLFAANFMAGRFNP 135 Query: 130 KGRYIRAWNPWREGEDNSGVAIIDCCMNTSLLFWASAETGDPRYRHIAEAHMDTVLEYFI 189 GR++RAWN D G +IID CMN SLL+WASAE+GD R+ HIA AH DTVL YFI Sbjct: 136 NGRFLRAWNG-----DMHGWSIIDTCMNLSLLYWASAESGDSRFAHIASAHADTVLNYFI 190 Query: 190 RADGSVYHIVNFNPDTGEVKEKLGGQGYGPESAWSRGAAWAIYGLTLAYHHSGKQEYLHA 249 R DGSV HIV F+P+TGE + GGQG P S WSRGA+WA+YG+ Y ++GK EYL A Sbjct: 191 REDGSVNHIVCFDPETGEFDQVRGGQGAAPNSGWSRGASWALYGMANTYRYTGKIEYLRA 250 Query: 250 AQRVADFFLTRLPEDHVPHWDFRAPGEI-GEIRDTXXXXXXXXXXXXXXXXXXDPESHMY 308 AQRVA +F+ LPEDHVPHWDFRA ++ E RDT + E +Y Sbjct: 251 AQRVAHYFIANLPEDHVPHWDFRASEQMEEEPRDTSAGAIAASGLIELAALLTEKEGRIY 310 Query: 309 RSGALKIMESLFRNYGTWDQPDEEGLLLHGTSNYPENRNIDVPLIYGDFFYVEALARLK 367 R A +I+ESL NYGTW +P E +L HGT + P +N+DV LIYGD+F+VEA+A+L Sbjct: 311 REAAERILESLSSNYGTWSEPSHEAILKHGTGHKPAGQNVDVSLIYGDYFFVEAIAKLN 369 >B3KCR7.1 B3KCR7_9BACI Glycosyl hydrolase family 88 Length = 397 Score = 316 bits (809), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 172/376 (45%), Positives = 218/376 (57%), Gaps = 22/376 (5%) Query: 11 WSKALEKTGINSQSIGAEFPHASQGGKYVLEAPDWWTAGFWPGMLWQFYGECGDESLKAI 70 W K +K + + + PHA++ G Y DWWT+GFWPGMLW Y K Sbjct: 16 WGKLEKKVTRMTGQLQGKVPHATKNGIYDSTRIDWWTSGFWPGMLWMMYDMTKQPQYKEW 75 Query: 71 AEHCEKRLDEVLDGYVRLDHDLGFMWTLTSVANYKLTGNEISRIRALKAANYLSARFNLK 130 A + RL+E L G HD+GF + T+V YKLTG+ +R R L AA L+ R+N Sbjct: 76 AWEWDIRLEEALIGDSNFHHDVGFQFLPTAVIKYKLTGDPDARRRGLLAATLLAGRYNPV 135 Query: 131 GRYIRAWN-----PWREGEDNSGVAIIDCCMNTSLLFWASAETGDPRYRHIAEAHMDTVL 185 GR+IRAWN W G N G +IID MN SLLFWAS E+GDPR+ HIA +TV Sbjct: 136 GRFIRAWNQVKPTSWNHG--NEGWSIIDSTMNLSLLFWASEESGDPRFAHIAREQANTVA 193 Query: 186 EYFIRADGSVYHIVNFNPDTGEVKEKLGGQGYGPESAWSRGAAWAIYGLTLAYHHSGKQE 245 E+FIR DGSV HI F+P TGE E LGGQG+GPESAWSRGAAWA++G+ Y ++G Sbjct: 194 EHFIRPDGSVRHICRFDPLTGEYMESLGGQGFGPESAWSRGAAWALHGMANTYRYTGDIR 253 Query: 246 YLHAAQRVADFFLTRLPEDHVPHWDFRA--------------PGEI-GEIRDTXXXXXXX 290 YL AA+RVA FF+ L ED +P WDFRA P ++ E RDT Sbjct: 254 YLDAAKRVAHFFVASLEEDSIPLWDFRAERKDLKRPDYDGTEPSQMPSEPRDTSAASCAA 313 Query: 291 XXXXXXXXXXXDPESHMYRSGALKIMESLFRNYGTWDQPDEEGLLLHGTSNYPENRNIDV 350 ES +YR AL+I++SL Y WD PD EG+L T + P +NI+V Sbjct: 314 SGLLEIADLVPQAESALYRGSALRIIDSLTNRYAEWDDPDYEGILKEATGHLPAGQNINV 373 Query: 351 PLIYGDFFYVEALARL 366 LIYGD++YVE+ A+L Sbjct: 374 SLIYGDYYYVESAAKL 389 >Q5WCV2.1 Q5WCV2_BACSK Unsaturated glucuronyl hydrolase Length = 480 Score = 298 bits (764), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 159/338 (47%), Positives = 210/338 (62%), Gaps = 8/338 (2%) Query: 30 PHAS-QGGKYVLEAPDWWTAGFWPGMLWQFYGECGDESLKAIAEHCEKRLDEVLDGY-VR 87 PHAS G+Y WWT+GF+PG+LW + G+ K AE ++++E + + Sbjct: 140 PHASDSSGRYDDMPVSWWTSGFYPGLLWLMHDVTGNAFFKNQAEGWSRKIEEAFQEHPII 199 Query: 88 LDHDLGFMWTLTSVANYKLTGNEISRIRALKAANYLSARFNLKGRYIRAWNPWREGEDNS 147 L HD+GF + LT+V Y+ TG+E LKAANYL+ RFN G +I+AWN G+D Sbjct: 200 LHHDVGFQFLLTAVMEYEQTGSEDGLRIGLKAANYLAGRFNPIGGFIKAWN----GDDKK 255 Query: 148 GVAIIDCCMNTSLLFWASAETGDPRYRHIAEAHMDTVLEYFIRADGSVYHIVNFNPDTGE 207 G AIIDC MN SLL+WAS T DP Y IA AH +T L++ +R DGS HI++FN TGE Sbjct: 256 GWAIIDCMMNISLLYWASRVTNDPSYGQIASAHANTTLQWGVRDDGSTSHILSFNSHTGE 315 Query: 208 VKEKLGGQGYGPESAWSRGAAWAIYGLTLAYHHSGKQEYLHAAQRVADFFLTRLPEDHVP 267 E +GGQGYG +SAWSRG AWA+YG A H+GK+ YLHAA+R+A FFL LPED+VP Sbjct: 316 FIESIGGQGYGSDSAWSRGNAWALYGFANACRHTGKRAYLHAAKRIAHFFLAALPEDNVP 375 Query: 268 HWDFRAPGEIGEIRDTXXXXXXXXXXXXXXXXXXDPESHMYRSGALKIMESLFRNYGTWD 327 +WDFR GE E RD+ E MYR A KI++SL +Y D Sbjct: 376 YWDFRCGGEEEEPRDSSAAAIAASGLLEIEAQVPQREKRMYRRAAEKILQSLSTHYLASD 435 Query: 328 QPDEEGLLLHGTSNYPENRNIDVPLIYGDFFYVEALAR 365 + E +L+ T N P+ R+I+V LIYGD+F+ EA+A+ Sbjct: 436 --ESEAILMKATGNAPQGRDINVSLIYGDYFFAEAIAK 471 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,433,508,313 Number of extensions: 102327087 Number of successful extensions: 209462 Number of sequences better than 1.0e-05: 299 Number of HSP's gapped: 208431 Number of HSP's successfully gapped: 299 Length of query: 375 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 238 Effective length of database: 1,770,017,148 Effective search space: 421264081224 Effective search space used: 421264081224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)