BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0015.p (450 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3T4.1 B3K3T4_9BACI Transporter 518 e-145 B1DEM3.1 B1DEM3_9BACL Transporter 471 e-131 A8MJZ8.1 A8MJZ8_ALKOO Transporter 295 5e-78 A8VWH9.1 A8VWH9_9BACI Transporter 275 1e-71 C4EU14.1 C4EU14_9BACT SNF family Na+-dependent transporter 266 2e-69 >B3K3T4.1 B3K3T4_9BACI Transporter Length = 451 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 267/438 (60%), Positives = 319/438 (72%), Gaps = 1/438 (0%) Query: 14 KKERFSSSGFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVLLAELA 73 K +RFSS+GFI AAIGSSVGLGNMWKFPYITGE+GGAA PVLLAELA Sbjct: 13 KNDRFSSAGFILAAIGSSVGLGNMWKFPYITGEHGGAAFFLLFIICLIVIGLPVLLAELA 72 Query: 74 IGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAFNGQLF 133 IGR+GRGSAATSF++ +LQV+APFLIL+FY+IVAGWTL+YA+++F+G L+ Sbjct: 73 IGRAGRGSAATSFVKAGGPKVFGQLGLLQVIAPFLILTFYVIVAGWTLHYAVMSFSGNLY 132 Query: 134 STSDYAGQFGSFISGYMPIVWQGVAMLITAGVVILGVSGGIEKFNKXXXXXXXXXXXXXX 193 S +D+ GQF +F GYMPI+WQ VA+ IT VV G+SGGIEKFNK Sbjct: 133 SNTDFGGQFTAFTEGYMPIIWQIVAIAITGWVVAKGISGGIEKFNKVLIPGLIILLIVLM 192 Query: 194 XXXXTLPGSGEGVSFFLKPDFSVLTAESALVALGHAFFSLSLGMGILLTYGSYVDKRQXX 253 TLPG+G GVSFFL PDFS L+ E+ALVALGHAFFSLSLGMGILLTYG+YVDKRQ Sbjct: 193 IRAVTLPGAGAGVSFFLNPDFSKLSPEAALVALGHAFFSLSLGMGILLTYGAYVDKRQSL 252 Query: 254 XXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSAMPXXXXXXXX 313 DL+YAFIAGLIIFPTT SFGI P+ GPSL+FIALPAAFSAMP Sbjct: 253 GPATLAIGAGDLIYAFIAGLIIFPTTASFGIEPNAGPSLVFIALPAAFSAMPLGAFFGGL 312 Query: 314 XXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGIPSAMSLGMFPEL 373 EVP S+ M+RW+W R +AV ++S + L+G+PSA+S G+ P L Sbjct: 313 FFILLAIAALTSAVSLLEVPTSYVMDRWHWGRTKAVVVISALVLLVGLPSALSFGIVPGL 372 Query: 374 T-FGGKALFDWMDFITSNIMLPLGGLLITIFAGYFWKGAAEAAGLQARWFRIWLFMLRYV 432 T GGK FDW+DFITSNI+LP+GGL+ T+FAGYFWK AA+AAGL+A WFR+WLFMLRY+ Sbjct: 373 TDIGGKNFFDWLDFITSNILLPIGGLITTLFAGYFWKKAADAAGLKAGWFRVWLFMLRYI 432 Query: 433 APVLVFLVLLHTSGILTF 450 APVLVFLVLLHT+G++TF Sbjct: 433 APVLVFLVLLHTTGLITF 450 >B1DEM3.1 B1DEM3_9BACL Transporter Length = 457 Score = 471 bits (1211), Expect = e-131, Method: Compositional matrix adjust. Identities = 252/441 (57%), Positives = 306/441 (69%), Gaps = 1/441 (0%) Query: 11 LSEKKERFSSSGFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVLLA 70 L++ +ERFS SGFIFAAIGS+VGLGNMWKFPYITG+ GGAA PVL+A Sbjct: 13 LADGEERFSKSGFIFAAIGSAVGLGNMWKFPYITGKYGGAAFFLMFIVCLLAVGLPVLMA 72 Query: 71 ELAIGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAFNG 130 ELAIGR GRGS +TSF R L + APFLILSFY +VAGWT+ YA+ +F+G Sbjct: 73 ELAIGRGGRGSVSTSFNRLTKQKGWGALGFLMIAAPFLILSFYSVVAGWTMYYAVRSFSG 132 Query: 131 QLFSTSDYAGQFGSFISGYMPIVWQGVAMLITAGVVILGVSGGIEKFNKXXXXXXXXXXX 190 QLF+T+DY+GQF +FI + P+ W +A+++TA V+I GVS GIEKFNK Sbjct: 133 QLFTTTDYSGQFNAFIGSWQPVWWMLLALVLTAAVIIKGVSAGIEKFNKILIPGLVILLL 192 Query: 191 XXXXXXXTLPGSGEGVSFFLKPDFSVLTAESALVALGHAFFSLSLGMGILLTYGSYVDKR 250 TL G+GEGVSFFL PDFS LTAESALVALGHAFFSLSLGMG ++TYG+YVDKR Sbjct: 193 VLVVRSLTLDGAGEGVSFFLSPDFSKLTAESALVALGHAFFSLSLGMGTMITYGAYVDKR 252 Query: 251 QXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSAMPXXXXX 310 Q DL+YAF+AGLIIFPT+F+FGI P QGP L+F+ALPAAF+AMP Sbjct: 253 QSLGAGTMAIGLGDLLYAFLAGLIIFPTSFAFGIEPGQGPGLVFVALPAAFAAMPLGSLF 312 Query: 311 XXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGIPSAMSL-GM 369 EVPV+F ER+ WSR AV +++++ FL+GIPSA+S+ G Sbjct: 313 GGLFFILLAVASLTSTVGLLEVPVAFIRERYGWSRGLAVTVITVLLFLVGIPSAVSVGGA 372 Query: 370 FPELTFGGKALFDWMDFITSNIMLPLGGLLITIFAGYFWKGAAEAAGLQARWFRIWLFML 429 L FGGK FDWMDFI SNIMLPLGGL++TIF G+ WK AAE AGL A WF IWLFM+ Sbjct: 373 VSGLDFGGKTFFDWMDFICSNIMLPLGGLIVTIFVGWVWKNAAEEAGLTAAWFGIWLFMV 432 Query: 430 RYVAPVLVFLVLLHTSGILTF 450 RY+AP+LV LVLL+++G L F Sbjct: 433 RYIAPILVVLVLLYSAGALDF 453 >A8MJZ8.1 A8MJZ8_ALKOO Transporter Length = 454 Score = 295 bits (756), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 178/452 (39%), Positives = 250/452 (55%), Gaps = 17/452 (3%) Query: 11 LSEKKERF---SSSGFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPV 67 L+E K+R S GFI AA GS+VGLGN+WKFPY+ G NGG A + Sbjct: 4 LNENKQRDQWGSKLGFIMAAAGSAVGLGNLWKFPYLAGSNGGGAFVFVYLVLILLVGFTM 63 Query: 68 LLAELAIGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIA 127 +LAE+ IGR+ + S+ ++ + L V+A FLILSFY +V GWT++Y + A Sbjct: 64 MLAEIVIGRNTQLSSVGAYKKLNAKWGWLGG--LGVLAGFLILSFYSVVGGWTISYIVKA 121 Query: 128 FNGQLFSTSDY---AGQFGSFISGYM-PIVWQGVAMLITAGVVILGVSGGIEKFNKXXXX 183 G F+T+D G FG I+G + P+V+ G+ M+IT +V+ GVSGGIEK K Sbjct: 122 ITGA-FNTADLDLLGGMFGGLIAGTLEPLVYHGIFMIITLLIVVGGVSGGIEKSTKIMMP 180 Query: 184 XXXXXXXXXXXXXXTLPGSGEGVSFFLKPDFSVLTAESALVALGHAFFSLSLGMGILLTY 243 TLPG+ EGV FFL PDFS LT L ALG FFSLSLGMG ++TY Sbjct: 181 ALFVMMIMVMIRSLTLPGAMEGVKFFLVPDFSKLTPSVLLAALGQVFFSLSLGMGCMITY 240 Query: 244 GSYVDKRQXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSA 303 GSY+ K + D A +AG I P F+FG P GP L+F+ LPA FS Sbjct: 241 GSYLGKDEDIVQSSMIIPALDTCIALLAGFTILPAVFAFGFDPSAGPGLLFVTLPAVFSK 300 Query: 304 MPXXXXXXXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGIPS 363 MP EV VS+ ++ + W R++A +I+S I F++G+ + Sbjct: 301 MPLGTLFAVVFFVLVLFAALTSSISLLEVTVSYMVDEFKWDRKKATYIMSGIIFIVGVAA 360 Query: 364 AMSLGMFPELTF-GGKALFDWMDFITSNIMLPLGGLLITIFAGYFW------KGAAEAAG 416 ++S+G + + GG+ +FD + FI NI+LPLGG+L+ IF G+ W K A+ Sbjct: 361 SLSMGPWSHIELIGGRNIFDTLGFIAENILLPLGGMLMCIFIGWVWGLENAIKEASNEGK 420 Query: 417 LQARWFRIWLFMLRYVAPVLVFLVLLHTSGIL 448 L + +W F+++Y+AP+ V +V L +SGIL Sbjct: 421 LAFKLAPVWGFLVKYIAPLAVLIVFLQSSGIL 452 >A8VWH9.1 A8VWH9_9BACI Transporter Length = 448 Score = 275 bits (702), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 171/452 (37%), Positives = 241/452 (53%), Gaps = 19/452 (4%) Query: 9 SLLSEKKERFSSSGFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVL 68 S L +++ S GFI AA+GS+VGLGN+W+F Y+ GE+GGAA P+L Sbjct: 2 SALKTREQWGSKLGFILAAMGSAVGLGNIWRFSYVAGESGGAAFLLIYIACIIAIGIPIL 61 Query: 69 LAELAIGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAF 128 +AE +IGR + SF L V + F+ LSFY ++AGW+L+Y L F Sbjct: 62 MAEFSIGRRAQSDVVGSFETIAPGKPWVLAGFLGVASSFIFLSFYGVIAGWSLHYFLQYF 121 Query: 129 NGQLFSTS-----DYAGQFGSFISG-YMPIVWQGVAMLITAGVVILGVSGGIEKFNKXXX 182 G + DY F +FIS P+ WQ + M +T +V G+ GIE +NK Sbjct: 122 TGGPAAGEAGGYGDY---FVNFISSPIQPLFWQFLFMALTIAIVFGGIKKGIEMWNKILM 178 Query: 183 XXXXXXXXXXXXXXXTLPGSGEGVSFFLKPDFSVLTAESA-LVALGHAFFSLSLGMGILL 241 TL G+GE +SF PD+S + + L ALG AFFSLSLGMG L+ Sbjct: 179 PALAILVIGLAIYSLTLGGAGEAMSFLFSPDWSAFSDPAVYLAALGQAFFSLSLGMGALI 238 Query: 242 TYGSYVDKRQXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAF 301 TYGSY+ K++ D ++A IAG++IFP F+ GI+PD GP L+FIALP F Sbjct: 239 TYGSYLSKKETLPSAAVSVASLDTLFAIIAGIMIFPVVFAVGISPDSGPGLVFIALPEIF 298 Query: 302 SAMPXXXXXXXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGI 361 + M EV V++ M R+ WSR++A I+ I FL+GI Sbjct: 299 AEMNGGLIFGFLFFFLLSAAAITSGVSLLEVAVAYFMRRFEWSRKKAAVIIGTIIFLIGI 358 Query: 362 PSAMSLGMFPELTFGGKALFDWMDFITSNIMLPLGGLLITIFAGYFWKGAAEAAGLQARW 421 PS++ G+ + GG + D DF+ SNI LPLGGL+I +F G+ WK + L+ Sbjct: 359 PSSLGNGVLNNVRIGGLEILDAADFLASNIFLPLGGLMIALFVGWGWK---KTDALRESD 415 Query: 422 F------RIWLFMLRYVAPVLVFLVLLHTSGI 447 F +W+F+LR++APV++ LV L+ GI Sbjct: 416 FGDTTIGHLWVFVLRFIAPVMIMLVFLNAIGI 447 >C4EU14.1 C4EU14_9BACT SNF family Na+-dependent transporter Length = 443 Score = 266 bits (681), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 171/438 (39%), Positives = 235/438 (53%), Gaps = 15/438 (3%) Query: 11 LSEKKERFSSS-GFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVLL 69 +S +E++ S GFI AA GS+VGLGN+W+FPY+TG +GG P++L Sbjct: 1 MSRGREQWGSKFGFIMAAAGSAVGLGNIWRFPYLTGVSGGGIFVVLYLILVAAIGMPIML 60 Query: 70 AELAIGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAFN 129 AE+AIGR G +A SF R VVA F+ILSFY +VAGWT+ Y +F Sbjct: 61 AEMAIGRGGGLNAVGSF-RKLKGGLWPMVGWAGVVAGFVILSFYGVVAGWTVAYMFKSF- 118 Query: 130 GQLFS---TSDYAGQFGSFISGYMPIV-WQGVAMLITAGVVILGVSGGIEKFNKXXXXXX 185 G L S A F +F + Y+ ++ +QG ML T +V GV GGIE++ K Sbjct: 119 GPLMSMAAAGGAAEAFQTFTNDYVQVIAYQGAFMLATIWIVYNGVGGGIERYCKVMMPSL 178 Query: 186 XXXXXXXXXXXXTLPGSGEGVSFFLKPDFSVLTAESALVALGHAFFSLSLGMGILLTYGS 245 TL G+ +GV F+LKPD S LTA + + ALG AFFSLS+GMG ++TYGS Sbjct: 179 YIILAILMVRSLTLEGAMKGVEFYLKPDISKLTARTVMGALGQAFFSLSIGMGCMITYGS 238 Query: 246 YVDKRQXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSAMP 305 Y+ R+ ++ A + G +IFP F+F + P GP LIF+ LPA F+ MP Sbjct: 239 YLSDRESLPSSVFIVTVMNVAVAILVGFVIFPAVFAFNMEPSSGPGLIFVTLPAVFARMP 298 Query: 306 XXXXXXXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGIPSAM 365 EV V++ + + W R RA WI+ L CFLLGIPSA+ Sbjct: 299 MGSLFSFLFFTLLFFAAVTSSVSLLEVCVAYMKDEFRWDRGRAAWIIGLACFLLGIPSAL 358 Query: 366 SLG-MFPELTFGGKALFDWMDFITSNIMLPLGGLLITIFAGYFW-KGAAE----AAGLQA 419 SLG FP + G MDF++SN++LPLGG+ I++FAG+ W +GA + L Sbjct: 359 SLGDHFPRVF--GMDFLGAMDFLSSNVLLPLGGIFISVFAGWSWFEGARQEVRRGGALPK 416 Query: 420 RWFRIWLFMLRYVAPVLV 437 W+ R +AP+ V Sbjct: 417 PLETFWVLACRILAPIAV 434 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.143 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,230,962,784 Number of extensions: 79853443 Number of successful extensions: 264385 Number of sequences better than 1.0e-05: 1567 Number of HSP's gapped: 258270 Number of HSP's successfully gapped: 1629 Length of query: 450 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 311 Effective length of database: 1,751,175,118 Effective search space: 544615461698 Effective search space used: 544615461698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)