BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0014.p (255 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4J8V3.1 A4J8V3_DESRM Short-chain dehydrogenase/reductase SDR 259 2e-67 C1I4Q3.1 C1I4Q3_9CLOT Short-chain dehydrogenase/reductase SDR 249 2e-64 B0MXP8.1 B0MXP8_9BACT Putative uncharacterized protein 248 4e-64 B9Y7N4.1 B9Y7N4_9FIRM Putative uncharacterized protein 230 1e-58 B2A7C1.1 B2A7C1_NATTJ Short-chain dehydrogenase/reductase SDR 229 2e-58 >A4J8V3.1 A4J8V3_DESRM Short-chain dehydrogenase/reductase SDR Length = 257 Score = 259 bits (661), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 133/256 (51%), Positives = 180/256 (70%), Gaps = 3/256 (1%) Query: 3 NLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQTLS 62 NLFDLSGK + A+A+AAQGADVALL R KL +EIE+ G++ ++ Sbjct: 2 NLFDLSGKVAVITGASSGLGADAARAYAAQGADVALLARRKTKLDQVVKEIEATGKKAIA 61 Query: 63 LGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKSIF 122 + CDV E+ ++ AV+ IL H+G+IDIL+NNAG+A GSVE + +WD +D N+K IF Sbjct: 62 VQCDVSNEENVKQAVEEILSHYGKIDILLNNAGIAIKGSVENMTVEQWDTAMDINVKGIF 121 Query: 123 LMSKHVIKPMKERRYGRIINMASICGMTGSKSFPL--HAYNASKGAVLSITRGMGASLAP 180 LM K+++ MK+R+YG+IIN+AS+ + K+ PL H+YN SK AVL +T GM SLA Sbjct: 122 LMCKYIVPQMKQRKYGKIINVASVNAIIADKAEPLVRHSYNTSKSAVLGLTTGMAVSLAQ 181 Query: 181 YGITVNAIGPSLFRTEMTERALY-QDSFLQLYNAQCPIGRPGNPEEINGAAIYFASDASS 239 YGITVNA+GP LF +EMT L+ ++SFLQ+YN+ CP RPG E+NG ++F+SDASS Sbjct: 182 YGITVNAVGPGLFESEMTADTLFKEESFLQMYNSLCPASRPGRKGELNGPILFFSSDASS 241 Query: 240 YTTGQTLYVDGGWTSV 255 YTTGQ + VDGG T+V Sbjct: 242 YTTGQFITVDGGLTAV 257 >C1I4Q3.1 C1I4Q3_9CLOT Short-chain dehydrogenase/reductase SDR Length = 260 Score = 249 bits (636), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 119/256 (46%), Positives = 175/256 (68%), Gaps = 3/256 (1%) Query: 3 NLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQTLS 62 N+FDL GK + A A+A +GA+V +L R +K+ + A+EIE+ G ++++ Sbjct: 5 NMFDLKGKIAVVTGASSGLGRDAALAYAKEGANVCVLARRIEKIESLAKEIEALGVESIA 64 Query: 63 LGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKSIF 122 + CDV E+E++ AV+ I+ +G++DIL+NNAG+A G VE L E EWD+ +DTN+K I+ Sbjct: 65 IKCDVTNEEEVKVAVETIVNKFGRVDILLNNAGIAVRGGVETLSEEEWDKSMDTNVKGIY 124 Query: 123 LMSKHVIKPMKERRYGRIINMASICGMTGSKS--FPLHAYNASKGAVLSITRGMGASLAP 180 L+SK+VI MK++ YG+++N++SI + K+ F H+YNASK AVL ++RGM AS A Sbjct: 125 LVSKYVIPVMKKQNYGKVVNVSSINALLADKADIFIRHSYNASKAAVLGLSRGMAASYAQ 184 Query: 181 YGITVNAIGPSLFRTEMTERALYQDS-FLQLYNAQCPIGRPGNPEEINGAAIYFASDASS 239 +GITVN++ P LF +EMTE L++ FL+ YN CP RP E+NG +YF+SDASS Sbjct: 185 FGITVNSVCPGLFESEMTEDTLFKSQEFLKSYNTLCPASRPAKKGELNGTILYFSSDASS 244 Query: 240 YTTGQTLYVDGGWTSV 255 Y TGQ + VDGG + V Sbjct: 245 YVTGQHIVVDGGLSIV 260 >B0MXP8.1 B0MXP8_9BACT Putative uncharacterized protein Length = 258 Score = 248 bits (633), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 124/259 (47%), Positives = 176/259 (67%), Gaps = 5/259 (1%) Query: 1 MNNLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQT 60 M+NLFDL+G+ + A+A+A QGADVALL R +KL T A EI + GR+ Sbjct: 1 MDNLFDLTGRVAVVTGASSGLGADAARAYAGQGADVALLARRVEKLKTVADEIAATGRKV 60 Query: 61 LSLGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKS 120 L +GCDV E+++ A+ ++ +G+IDIL+NNAGVA G+VE+L EWD+ ++TN++ Sbjct: 61 LVVGCDVTDEEQVRKAIGQVVARFGKIDILLNNAGVAVPGTVEQLTTDEWDKEMNTNVRG 120 Query: 121 IFLMSKHVIKPMKERRYGRIINMASICGMTGSKSFPL---HAYNASKGAVLSITRGMGAS 177 +L+ K+V+ M+ERRYG+I+N++S+ + KS PL H YNASK AVL +T GM AS Sbjct: 121 AYLVCKYVVPHMRERRYGKIVNISSVNALIADKS-PLLARHVYNASKAAVLGLTIGMAAS 179 Query: 178 LAPYGITVNAIGPSLFRTEMTERALYQ-DSFLQLYNAQCPIGRPGNPEEINGAAIYFASD 236 GITVNA+ P LF +EMTE L++ + F+++YN P+ RPG E+NG +YF+SD Sbjct: 180 YGVDGITVNAVCPGLFESEMTEDTLFKNEEFMRMYNFTDPMSRPGRRGELNGTILYFSSD 239 Query: 237 ASSYTTGQTLYVDGGWTSV 255 ASSY TGQ + VDGG + V Sbjct: 240 ASSYVTGQHVVVDGGASIV 258 >B9Y7N4.1 B9Y7N4_9FIRM Putative uncharacterized protein Length = 281 Score = 230 bits (586), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 120/255 (47%), Positives = 170/255 (66%), Gaps = 3/255 (1%) Query: 4 LFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQTLSL 63 +F+L+GK + A+A+A GA+VALL R DKL A E+ + G L + Sbjct: 27 MFELTGKIAVVTGASSGLGADAARAYAEAGANVALLARRQDKLEALAEELRAKGIDALPV 86 Query: 64 GCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKSIFL 123 GCDV E IE AVQ +++H+G+IDIL+NNAGVA G V+ L + +WDR ++ N+K I++ Sbjct: 87 GCDVTDEASIEKAVQTVIEHFGRIDILLNNAGVAVRGGVDTLSQEDWDRSMNINVKGIYM 146 Query: 124 MSKHVIKPMKERRYGRIINMASICGMTGSKS--FPLHAYNASKGAVLSITRGMGASLAPY 181 M K+VI MK R YG+I+N++S+ + G K+ F HAYN SK AVL +T GM S + Sbjct: 147 MCKYVIPHMKARNYGKIVNISSVNALIGDKNDMFIRHAYNTSKAAVLGLTLGMACSYGQF 206 Query: 182 GITVNAIGPSLFRTEMTERALYQ-DSFLQLYNAQCPIGRPGNPEEINGAAIYFASDASSY 240 ITVNA+ P LF +EMT+ L++ ++FLQ Y+AQCP+ RPG E++G ++F+SDASSY Sbjct: 207 NITVNAVCPGLFESEMTQNTLFKSEAFLQGYSAQCPMSRPGRRGEVSGPILFFSSDASSY 266 Query: 241 TTGQTLYVDGGWTSV 255 TGQ + VDGG + V Sbjct: 267 VTGQHVVVDGGTSLV 281 >B2A7C1.1 B2A7C1_NATTJ Short-chain dehydrogenase/reductase SDR Length = 259 Score = 229 bits (583), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 106/258 (41%), Positives = 168/258 (65%), Gaps = 3/258 (1%) Query: 1 MNNLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIES---AG 57 +N LFDL+GK +K A GA+++++ R +KL +I++ + Sbjct: 2 INELFDLTGKTAIVTGASSGLGWRFSKVLAQAGANLSIVARRKEKLEQLREDIKNTVNSE 61 Query: 58 RQTLSLGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTN 117 +++L+L CDV +E E++ V+ Q +G+IDIL+NNAG+++ VE+LE+ +WD+V+DTN Sbjct: 62 KESLALQCDVQKEAEVKDVVEKTEQEFGKIDILINNAGISALAPVEDLEQEDWDKVMDTN 121 Query: 118 LKSIFLMSKHVIKPMKERRYGRIINMASICGMTGSKSFPLHAYNASKGAVLSITRGMGAS 177 L +F +KH + MK + G+I+N+AS+ G G+ FP Y+ASKG V+++T+ + Sbjct: 122 LTGVFFFAKHAARKMKANKSGKIVNIASMFGKVGNTFFPATPYHASKGGVITLTQALAGE 181 Query: 178 LAPYGITVNAIGPSLFRTEMTERALYQDSFLQLYNAQCPIGRPGNPEEINGAAIYFASDA 237 LA +GI VNAIGP F +EMT+ A +SF + ++CP RPG E++GA I+ AS++ Sbjct: 182 LAQHGIQVNAIGPGFFESEMTQDAFGDESFHEYVRSRCPSQRPGREGELDGALIFLASES 241 Query: 238 SSYTTGQTLYVDGGWTSV 255 SSY GQT++VDGGWT+V Sbjct: 242 SSYVNGQTIFVDGGWTAV 259 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,366,064,918 Number of extensions: 52704347 Number of successful extensions: 238270 Number of sequences better than 1.0e-05: 28797 Number of HSP's gapped: 196365 Number of HSP's successfully gapped: 28877 Length of query: 255 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 122 Effective length of database: 1,807,701,208 Effective search space: 220539547376 Effective search space used: 220539547376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)