BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0469.0003.p (158 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A7GI36.1 A7GI36_CLOBL Radical SAM domain protein 128 2e-28 B1IMY4.1 B1IMY4_CLOBK Radical SAM domain protein 128 2e-28 B8I5G7.1 B8I5G7_CLOCE Radical SAM domain protein 123 5e-27 C3KY36.1 C3KY36_CLOB6 Radical SAM-family protein 121 3e-26 B1QRB1.1 B1QRB1_CLOBO Radical SAM-family protein 121 3e-26 >A7GI36.1 A7GI36_CLOBL Radical SAM domain protein Length = 468 Score = 128 bits (321), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 66/147 (44%), Positives = 96/147 (65%), Gaps = 6/147 (4%) Query: 4 KENPLIYSFENMDKYYFFDVNKNKIINVDEETYQII----NDPDAVRSSQHPVIQKLKDQ 59 K+N + F+ + Y +DVN N + V +ETY + D +A++ VI LK++ Sbjct: 2 KQNAFFHLFKTRNCCYIYDVNTNLFLKVPKETYSALENYNGDLEALKDDS--VILNLKEK 59 Query: 60 GYLSNNRVEEIKHSNGKMLDELLGYNLKKIILQVTQNCNFRCNYCVYSGSYVNRVHSNKR 119 GYLS+NR EEI+HS L+ +L +L + LQ+TQ CN RC+YCVYSGSY+NR HS+K Sbjct: 60 GYLSSNRAEEIEHSLTDALESILDNSLNTLTLQITQECNLRCSYCVYSGSYLNRSHSHKI 119 Query: 120 MDFATAQKGIDFLIEHSKDAKDIDISF 146 M TA+KGIDF+I HSK++ ++ +F Sbjct: 120 MSIDTAKKGIDFIINHSKNSPTVNFAF 146 >B1IMY4.1 B1IMY4_CLOBK Radical SAM domain protein Length = 468 Score = 128 bits (321), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 66/147 (44%), Positives = 96/147 (65%), Gaps = 6/147 (4%) Query: 4 KENPLIYSFENMDKYYFFDVNKNKIINVDEETYQII----NDPDAVRSSQHPVIQKLKDQ 59 K+N + F+ + Y +DVN N + V +ETY + D +A++ VI LK++ Sbjct: 2 KQNAFFHLFKTRNCCYIYDVNTNLFLKVPKETYSALENYNGDLEALKDDS--VILNLKEK 59 Query: 60 GYLSNNRVEEIKHSNGKMLDELLGYNLKKIILQVTQNCNFRCNYCVYSGSYVNRVHSNKR 119 GYLS+NR EEI+HS L+ +L +L + LQ+TQ CN RC+YCVYSGSY+NR HS+K Sbjct: 60 GYLSSNRAEEIEHSLTDALESILDNSLNTLTLQITQECNLRCSYCVYSGSYLNRSHSHKI 119 Query: 120 MDFATAQKGIDFLIEHSKDAKDIDISF 146 M TA+KGIDF+I HSK++ ++ +F Sbjct: 120 MSIDTAKKGIDFIINHSKNSPTVNFAF 146 >B8I5G7.1 B8I5G7_CLOCE Radical SAM domain protein Length = 462 Score = 123 bits (309), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 64/140 (45%), Positives = 82/140 (58%), Gaps = 4/140 (2%) Query: 7 PLIYSFENMDKYYFFDVNKNKIINVDEETYQIINDPDAVRSSQHPVIQKLKDQGYLSNNR 66 P I+ FE YF+DVNKN II + EE +N D +HP +Q L ++G+LS+ Sbjct: 7 PFIHMFETNGSKYFYDVNKNSIIGISEELADALNKNDM----EHPKVQSLIEKGFLSDKH 62 Query: 67 VEEIKHSNGKMLDELLGYNLKKIILQVTQNCNFRCNYCVYSGSYVNRVHSNKRMDFATAQ 126 VE I H K L+ L + I +QVTQ CN RC YC YSG Y NR HSN++M A Sbjct: 63 VETIIHPEDKFLEYHLNNKINMITIQVTQACNLRCTYCPYSGKYNNRQHSNRKMSLEMAI 122 Query: 127 KGIDFLIEHSKDAKDIDISF 146 +GIDFL EHS D ++I F Sbjct: 123 RGIDFLWEHSIDCDYVNIGF 142 >C3KY36.1 C3KY36_CLOB6 Radical SAM-family protein Length = 476 Score = 121 bits (303), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 62/142 (43%), Positives = 86/142 (60%), Gaps = 3/142 (2%) Query: 8 LIYSFENMDKYYFFDVNKNKIINVDEETYQIINDPDAVRSSQHPV---IQKLKDQGYLSN 64 I+ FE + YF+DVN N+I+N+ E Y+ +N+ D V ++ LKD GYL Sbjct: 6 FIHLFETPLEKYFYDVNMNEIVNIPETVYKYLNNGDMSDEDISIVEKYVKNLKDNGYLKK 65 Query: 65 NRVEEIKHSNGKMLDELLGYNLKKIILQVTQNCNFRCNYCVYSGSYVNRVHSNKRMDFAT 124 RVE +K+ K + + ++LQVTQNCN RC YCVYSG Y RVH+ RMD Sbjct: 66 KRVETVKNPLTKCAKCYIDNKINYLVLQVTQNCNLRCEYCVYSGGYDTRVHNKSRMDIGI 125 Query: 125 AQKGIDFLIEHSKDAKDIDISF 146 A+KGIDFLI+HS D +++ + F Sbjct: 126 AKKGIDFLIKHSSDCEELKLGF 147 >B1QRB1.1 B1QRB1_CLOBO Radical SAM-family protein Length = 476 Score = 121 bits (303), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 62/142 (43%), Positives = 86/142 (60%), Gaps = 3/142 (2%) Query: 8 LIYSFENMDKYYFFDVNKNKIINVDEETYQIINDPDAVRSSQHPV---IQKLKDQGYLSN 64 I+ FE + YF+DVN N+I+N+ E Y+ +N+ D V ++ LKD GYL Sbjct: 6 FIHLFETPLEKYFYDVNMNEIVNIPETVYKYLNNGDMSDEDISIVEKYVKNLKDNGYLKK 65 Query: 65 NRVEEIKHSNGKMLDELLGYNLKKIILQVTQNCNFRCNYCVYSGSYVNRVHSNKRMDFAT 124 RVE +K+ K + + ++LQVTQNCN RC YCVYSG Y RVH+ RMD Sbjct: 66 KRVETVKNPLTKCAKCYIDNKINYLVLQVTQNCNLRCEYCVYSGGYDTRVHNKSRMDIGI 125 Query: 125 AQKGIDFLIEHSKDAKDIDISF 146 A+KGIDFLI+HS D +++ + F Sbjct: 126 AKKGIDFLIKHSSDCEELKLGF 147 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 956,764,010 Number of extensions: 37670901 Number of successful extensions: 124948 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 124998 Number of HSP's successfully gapped: 52 Length of query: 158 Length of database: 3,060,696,203 Length adjustment: 119 Effective length of query: 39 Effective length of database: 1,939,595,418 Effective search space: 75644221302 Effective search space used: 75644221302 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)