BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0469.0002.p (321 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1FV60.1 C1FV60_CLOBJ Radical SAM domain protein 248 8e-64 B1IMY4.1 B1IMY4_CLOBK Radical SAM domain protein 244 1e-62 A7GI36.1 A7GI36_CLOBL Radical SAM domain protein 244 1e-62 B8I6T6.1 B8I6T6_CLOCE Radical SAM domain protein 238 6e-61 B1QB36.1 B1QB36_CLOBO Radical SAM-family protein 236 2e-60 >C1FV60.1 C1FV60_CLOBJ Radical SAM domain protein Length = 477 Score = 248 bits (632), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 128/311 (41%), Positives = 193/311 (62%), Gaps = 4/311 (1%) Query: 2 NLIIQCMDYAIKAAEGKNVSFSLTTNGSLLNETNVKRLLGYNLRITVSMDGPQEIHDKHR 61 + + +C++YA + EGK V F++TTNG+L NE +K L N RI +S+DGP+EIHDK+R Sbjct: 157 DFVKECVNYAKEKGEGKTVEFNMTTNGTLFNEERLKFLSENNFRIMISLDGPKEIHDKNR 216 Query: 62 TFASTGCGTFNAVYDNIKRIVEKYPD-IKKNLSFSMVMDPTAQFNCLDDFIASEEEFFNE 120 TF + G GTF+ + NI+ I KYP + +N+ F+ V+D + C F + + + Sbjct: 217 TFLN-GEGTFDKIMKNIEMIKYKYPKFLDENIDFNAVIDGKSDVVCTTKFFINYDTV-KD 274 Query: 121 SAVIGSFVSNNYRN-DRVQFSENFVGEWEYSKFKYLLFVLGKVSKANNSQLIRAAYREHF 179 V SFVS+ Y+ ++ E + + Y FK LL+ LGK+ + + S+L + + Sbjct: 275 LKVRTSFVSDTYKKIEQFTVEEENMIKIGYEGFKVLLWKLGKLDEKHVSKLFQGNFNNVK 334 Query: 180 EYISNSHRNIAPLAKQDHHSGPCVPGQIRLFMSTDAHFYPCERVSETSDIMRIGNIEEGF 239 + + L K+ HH GPC+PG RLF++ D F+PCE+V+E S +M+IGNI +GF Sbjct: 335 NLLHDKKYTYKKLNKKGHHGGPCIPGVTRLFVTVDGKFFPCEKVNEKSKVMQIGNISDGF 394 Query: 240 FFDKIEKLLNVGQLTEESCKECYAFRNCRVCASLSDDGNQLSKEAKQKACRDVRYNFDEM 299 +KI L+NVG+LTE CK+C+AFR C +C++ +DD +LSKE K C +VR + D+M Sbjct: 395 DIEKIRNLMNVGKLTENKCKKCWAFRYCFLCSAFADDNTKLSKEKKTSRCTEVRASVDDM 454 Query: 300 LKDVCTLKECG 310 K CTLKE G Sbjct: 455 FKTYCTLKEFG 465 >B1IMY4.1 B1IMY4_CLOBK Radical SAM domain protein Length = 468 Score = 244 bits (622), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 129/315 (40%), Positives = 200/315 (63%), Gaps = 5/315 (1%) Query: 2 NLIIQCMDYAIKAAEGKNVSFSLTTNGSLLNETNVKRLLGYNLRITVSMDGPQEIHDKHR 61 N I +C+ YA + EGK ++F++T NG+LL + +K + + + +SMDGP+EIHDK+R Sbjct: 156 NFIKECVAYAKEKGEGKKITFNMTINGTLLTDEIIKFMKDNEVSLLISMDGPKEIHDKNR 215 Query: 62 TFASTGCGTFNAVYDNIKRIVEKYPD-IKKNLSFSMVMDPTAQFNCLDDFIASEEEFFNE 120 F S G G+F+ + +NI +I K+P+ ++KN+ F++VMD F+C+++F A+ + + Sbjct: 216 VF-SNGMGSFDKIMNNINKIKVKFPEYVEKNVGFNIVMDGQNDFSCINNFFANYDTV-KD 273 Query: 121 SAVIGSFVSNNYRNDRVQFSENFVGEWEYSKFKYLLFVLGKVSKANNSQLIRAAYREHFE 180 S V + VS + SE F+ + +Y +FK LL LGK+ + S+L+ + + EH Sbjct: 274 SRVNATDVSTLGALEEKAISEEFIQDEKYERFKILLHKLGKLDEKYISKLLLSQF-EHIN 332 Query: 181 YISNSHRNI-APLAKQDHHSGPCVPGQIRLFMSTDAHFYPCERVSETSDIMRIGNIEEGF 239 + N R + K+ H GPC+PG RLFM+ D F PCERV+E S+ M IGN+ GF Sbjct: 333 HTLNVKREANEKVPKKMHPGGPCIPGLNRLFMTVDGKFCPCERVNEESEAMNIGNVNTGF 392 Query: 240 FFDKIEKLLNVGQLTEESCKECYAFRNCRVCASLSDDGNQLSKEAKQKACRDVRYNFDEM 299 +KI+KL+NVG++T++ CK C+AFR C +CA+ +DD N+LS + K C DV+ N +EM Sbjct: 393 EIEKIKKLINVGEMTKQKCKNCWAFRYCYLCAAFADDMNELSGVKRAKFCNDVKRNTEEM 452 Query: 300 LKDVCTLKECGLKKE 314 K CT+ E G K E Sbjct: 453 FKVYCTMMEFGYKFE 467 >A7GI36.1 A7GI36_CLOBL Radical SAM domain protein Length = 468 Score = 244 bits (622), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 128/315 (40%), Positives = 201/315 (63%), Gaps = 5/315 (1%) Query: 2 NLIIQCMDYAIKAAEGKNVSFSLTTNGSLLNETNVKRLLGYNLRITVSMDGPQEIHDKHR 61 N I +C+ YA + EGK ++F++T NG+LL + +K + + + +SMDGP+EIHDK+R Sbjct: 156 NFIKECVAYAKEKGEGKKITFNMTINGTLLTDEIIKFMKDNEVSLLISMDGPKEIHDKNR 215 Query: 62 TFASTGCGTFNAVYDNIKRIVEKYPD-IKKNLSFSMVMDPTAQFNCLDDFIASEEEFFNE 120 F S G G+F+ + +N+ +I K+P+ ++KN+ F++VMD F+C+++F A+ + + Sbjct: 216 VF-SNGMGSFDKIMNNVNKIKVKFPEYVEKNVGFNIVMDGQNDFSCINNFFANYDTV-KD 273 Query: 121 SAVIGSFVSNNYRNDRVQFSENFVGEWEYSKFKYLLFVLGKVSKANNSQLIRAAYREHFE 180 S V + VS + SE+F+ + +Y +FK LL LGK+ + S+L+ + + EH Sbjct: 274 SRVNATDVSTLGALEEKAISEDFIQDEKYERFKILLHKLGKLDEKYISKLLLSQF-EHIN 332 Query: 181 YISNSHRNI-APLAKQDHHSGPCVPGQIRLFMSTDAHFYPCERVSETSDIMRIGNIEEGF 239 + N R + K+ H GPC+PG RLFM+ D F PCERV+E S+ M IGN+ GF Sbjct: 333 HTLNVKREANEKVPKKMHPGGPCIPGLNRLFMTVDGKFCPCERVNEESEAMNIGNVNTGF 392 Query: 240 FFDKIEKLLNVGQLTEESCKECYAFRNCRVCASLSDDGNQLSKEAKQKACRDVRYNFDEM 299 +KI+KL+NVG++T++ CK C+AFR C +CA+ +DD N+LS + K C DV+ N +EM Sbjct: 393 EIEKIKKLINVGEMTKQKCKNCWAFRYCYLCAAFADDMNELSGVKRAKFCNDVKRNTEEM 452 Query: 300 LKDVCTLKECGLKKE 314 K CT+ E G K E Sbjct: 453 FKVYCTMMEFGYKFE 467 >B8I6T6.1 B8I6T6_CLOCE Radical SAM domain protein Length = 486 Score = 238 bits (607), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 121/310 (39%), Positives = 187/310 (60%), Gaps = 5/310 (1%) Query: 3 LIIQCMDYAIKAAEGKNVSFSLTTNGSLLNETNVKRLLGYNLRITVSMDGPQEIHDKHRT 62 I C++Y + AEGKN+ +LTTN +LL E V +N+ + +S+DG E+H+++R Sbjct: 166 FIKDCIEYFEEKAEGKNIHLNLTTNATLLTEEIVDYFHKHNVHLLISLDGSSEVHNRNRV 225 Query: 63 FASTGCGTFNAVYDNIKRIVEKYPD-IKKNLSFSMVMDPTAQFNCLDDFIASEEEFFNES 121 + GTF V +N++ I K+PD + N++F+ V+DP +F+C+++F E E+ Sbjct: 226 YRDGAKGTFEKVMENLEMIKTKFPDYLSTNIAFNAVLDPQNEFSCVNNFFTGFE-LAKEA 284 Query: 122 AVIGSFVSNNYRNDRVQFSENFVGEWEYSKFKYLLFVLGKVSKANNSQLIRAAYREH-FE 180 +++ S +++ YR D V+ S N++ E EY FK L LG + + S+L+ Y + Sbjct: 285 SIMASEITDLYRKDSVKTSGNYISEIEYEIFKIFLSKLGYLDEKYTSKLLSRQYNDRKTR 344 Query: 181 YISNSHRNIAPLAKQDHHSGPCVPGQIRLFMSTDAHFYPCERVSETSDIMRIGNIEEGFF 240 Y R P + HH GPCVPG RLF+ + +FYPCERVSE S M++GNI++GF Sbjct: 345 YRLLQMRQEIP--DKVHHGGPCVPGAQRLFVDVEGNFYPCERVSEASPQMKVGNIDDGFN 402 Query: 241 FDKIEKLLNVGQLTEESCKECYAFRNCRVCASLSDDGNQLSKEAKQKACRDVRYNFDEML 300 DKI LLN+G+++E C C+A C +CA +D+ + LS E K C +V Y D +L Sbjct: 403 IDKIRTLLNIGKISENKCINCWALTFCNLCAVAADENDGLSTEKKVSRCSNVIYTADSIL 462 Query: 301 KDVCTLKECG 310 KD+CTL+E G Sbjct: 463 KDICTLREFG 472 >B1QB36.1 B1QB36_CLOBO Radical SAM-family protein Length = 477 Score = 236 bits (602), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 130/311 (41%), Positives = 192/311 (61%), Gaps = 4/311 (1%) Query: 2 NLIIQCMDYAIKAAEGKNVSFSLTTNGSLLNETNVKRLLGYNLRITVSMDGPQEIHDKHR 61 + + +C++YA + EGK V F++TTNG+L NE +K L N RI +S+DGP+EIHDK+R Sbjct: 157 DFVRECVNYAKEKGEGKTVEFNMTTNGTLFNEERLKFLSENNFRIMISLDGPKEIHDKNR 216 Query: 62 TFASTGCGTFNAVYDNIKRIVEKYPD-IKKNLSFSMVMDPTAQFNCLDDFIASEEEFFNE 120 TF + G GTF+ + NI+ I KYP + +N+ F+ V+D + C D F + + Sbjct: 217 TFLN-GEGTFDKIMKNIEMIKSKYPKFLDENIDFNAVIDGKSDVACTDKFFINYNTV-KD 274 Query: 121 SAVIGSFVSNNYRN-DRVQFSENFVGEWEYSKFKYLLFVLGKVSKANNSQLIRAAYREHF 179 V SFVS+ Y+ ++ E + + Y +FK LL+ LGK+ + + S+L + + Sbjct: 275 LKVRTSFVSDTYKKIEQFTVEEENMIKIGYERFKLLLWKLGKLDEKHVSKLFQGNFNNVK 334 Query: 180 EYISNSHRNIAPLAKQDHHSGPCVPGQIRLFMSTDAHFYPCERVSETSDIMRIGNIEEGF 239 + + L K+ HH GPC+PG RLF+S D F+PCE+V+E S +M+IGNI +GF Sbjct: 335 NLLHDKKSTYKKLNKKGHHGGPCIPGVTRLFVSVDGKFFPCEKVNEKSKVMQIGNISDGF 394 Query: 240 FFDKIEKLLNVGQLTEESCKECYAFRNCRVCASLSDDGNQLSKEAKQKACRDVRYNFDEM 299 +KI L+NVG+LTE CK+C+AFR C +C++ +DD +LSKE K C VR + D M Sbjct: 395 DIEKIRNLMNVGKLTENKCKKCWAFRYCFLCSAFADDNTKLSKEKKTSRCTHVRASIDNM 454 Query: 300 LKDVCTLKECG 310 K CTLKE G Sbjct: 455 FKTYCTLKEFG 465 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,819,544,464 Number of extensions: 73048888 Number of successful extensions: 182052 Number of sequences better than 1.0e-05: 81 Number of HSP's gapped: 182154 Number of HSP's successfully gapped: 82 Length of query: 321 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 186 Effective length of database: 1,788,859,178 Effective search space: 332727807108 Effective search space used: 332727807108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)