BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0462.0006.p (201 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q24S33.1 Q24S33_DESHY Putative uncharacterized protein 87 7e-16 B8FWN6.1 B8FWN6_DESHD Transcriptional regulator, TetR family 87 7e-16 A8VX80.1 A8VX80_9BACI Transcriptional regulator, TetR family 74 8e-12 B4AM72.1 B4AM72_BACPU YsiA 73 1e-11 Q2B6Q6.1 Q2B6Q6_9BACI YsiA 73 1e-11 >Q24S33.1 Q24S33_DESHY Putative uncharacterized protein Length = 197 Score = 87.4 bits (215), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 58/188 (30%), Positives = 94/188 (50%), Gaps = 14/188 (7%) Query: 8 KHTAILDAAYELFGSGGFYETKISEVAERAGIAKGTVYLYFKNKEELFMAVTRRDCEGFL 67 K+ ILDAA E F G++ ++S++A AG+A GT+YLYFKNKE++ + + F+ Sbjct: 8 KYERILDAAIEAFAEVGYHSCQVSKIARIAGVADGTIYLYFKNKEDILIRLFTERMGDFI 67 Query: 68 QQLEAKLQACSSLTGKLSVIAEHHLFYYYERK---QHTKLFFRAPNNNPELVAYMALFME 124 Q + +++++C +L I HL + + T++ R PN+N L + +A+ + Sbjct: 68 QLIRSRMESCQDTRSRLRAIVHTHLTHMENNRALATVTQVELRQPNSN--LYSAIAVPLR 125 Query: 125 EYMKAVVMVLLEGGASE---PEL----MAQSYIGMLDRLKMDILFDPFFTEEEAHKRAKF 177 EY + V V+ EG + P L Q G +DR D +F +E K A Sbjct: 126 EYFRLVEEVIEEGIKQQEIIPSLNIHVARQMIFGTIDRATTDWVFAR--SERSLSKEADC 183 Query: 178 AASLFIGG 185 LF G Sbjct: 184 MLELFSGA 191 >B8FWN6.1 B8FWN6_DESHD Transcriptional regulator, TetR family Length = 197 Score = 87.4 bits (215), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 58/188 (30%), Positives = 94/188 (50%), Gaps = 14/188 (7%) Query: 8 KHTAILDAAYELFGSGGFYETKISEVAERAGIAKGTVYLYFKNKEELFMAVTRRDCEGFL 67 K+ ILDAA E F G++ ++S++A AG+A GT+YLYFKNKE++ + + F+ Sbjct: 8 KYERILDAAIEAFAEVGYHSCQVSKIARIAGVADGTIYLYFKNKEDILIRLFTERMGDFI 67 Query: 68 QQLEAKLQACSSLTGKLSVIAEHHLFYYYERK---QHTKLFFRAPNNNPELVAYMALFME 124 Q + +++++C +L I HL + + T++ R PN+N L + +A+ + Sbjct: 68 QLIRSRMESCQDTRSRLRAIVHTHLTHMENNRALATVTQVELRQPNSN--LYSAIAVPLR 125 Query: 125 EYMKAVVMVLLEGGASE---PEL----MAQSYIGMLDRLKMDILFDPFFTEEEAHKRAKF 177 EY + V V+ EG + P L Q G +DR D +F +E K A Sbjct: 126 EYFRLVEEVIEEGIKQQEIIPSLNIHVARQMIFGTIDRATTDWVFAR--SERSLSKEADC 183 Query: 178 AASLFIGG 185 LF G Sbjct: 184 MLELFSGA 191 >A8VX80.1 A8VX80_9BACI Transcriptional regulator, TetR family Length = 204 Score = 73.9 bits (180), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 44/144 (30%), Positives = 82/144 (56%), Gaps = 10/144 (6%) Query: 8 KHTAILDAAYELFGSGGFYETKISEVAERAGIAKGTVYLYFKNKEELFMAVTRRDCEGFL 67 K+ AI+DAA +F G++ +K+S++A++AG+A GT+YLYFKNKE++ +++ F+ Sbjct: 8 KYDAIIDAAVIVFAQNGYHLSKVSKIAKQAGVADGTIYLYFKNKEDILISMFEEKMSVFV 67 Query: 68 QQLEAKLQACSSLTGKLSVIAEHHLFYYYERKQH----TKLFFRAPNNNPELVAYMALFM 123 +++ ++ +S+ KL ++ E H F E T+L R N N + + + Sbjct: 68 DRIKHEINRVTSIEKKLEIMIEMH-FTQLELDPDLAIVTQLELRQSNTN--MRYQVNEVL 124 Query: 124 EEYMKAVVMVLLEG---GASEPEL 144 + Y+ + +L EG G +P+L Sbjct: 125 KGYLGLIDAILEEGIRQGVLDPDL 148 >B4AM72.1 B4AM72_BACPU YsiA Length = 194 Score = 73.2 bits (178), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 41/137 (29%), Positives = 79/137 (57%), Gaps = 7/137 (5%) Query: 8 KHTAILDAAYELFGSGGFYETKISEVAERAGIAKGTVYLYFKNKEELFMAVTRRDCEGFL 67 K+ I+DAA + G++++++S++A++AG+A GT+YLYFKNKE++ +++ + F+ Sbjct: 7 KYMQIIDAAVVVIAENGYHQSQVSKIAKQAGVADGTIYLYFKNKEDILISLFKEKMGQFI 66 Query: 68 QQLEAKLQACSSLTGKLSVIAEHHLFYYYERKQH----TKLFFRAPNNNPELVAYMALFM 123 +++E +Q S KL ++ E H F + H T+L R +N EL + + Sbjct: 67 ERMETDIQKKPSAKEKLLLLIEEH-FRMLAQNHHLALVTQLELR--QSNLELRQKINEVL 123 Query: 124 EEYMKAVVMVLLEGGAS 140 + Y+ + +L EG + Sbjct: 124 KGYLNMLESILAEGKKT 140 >Q2B6Q6.1 Q2B6Q6_9BACI YsiA Length = 194 Score = 73.2 bits (178), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 43/144 (29%), Positives = 79/144 (54%), Gaps = 10/144 (6%) Query: 8 KHTAILDAAYELFGSGGFYETKISEVAERAGIAKGTVYLYFKNKEELFMAVTRRDCEGFL 67 K+ I++AA + G+++ ++S++A +AG+A GT+YLYFKNKE++ +++ F+ Sbjct: 7 KYMQIIEAAVVVIAENGYHQAQVSKIARQAGVADGTIYLYFKNKEDILISLFEEKMGSFV 66 Query: 68 QQLEAKLQACSSLTGKLSVIAEHHLFYYYERKQH----TKLFFRAPNNNPELVAYMALFM 123 +++E + S T KL ++ E+H F H T+L R N + L + + Sbjct: 67 EKIEQNIAGNDSATEKLLMMVENH-FKLLSEDHHLAIVTQLELRQSNKDLRL--KINDVL 123 Query: 124 EEYMKAVVMVL---LEGGASEPEL 144 + Y+KA+ +L +E G P L Sbjct: 124 KGYLKAIDQILASGIETGEFSPSL 147 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,116,140,512 Number of extensions: 40404329 Number of successful extensions: 117253 Number of sequences better than 1.0e-05: 644 Number of HSP's gapped: 117513 Number of HSP's successfully gapped: 645 Length of query: 201 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 72 Effective length of database: 1,845,385,268 Effective search space: 132867739296 Effective search space used: 132867739296 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)