BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0462.0003.p (246 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLB3.1 B3KLB3_9BACI Glycerophosphodiester phosphodiesterase EC... 319 1e-85 B1DL22.1 B1DL22_9BACL Glycerophosphodiester phosphodiesterase EC... 251 4e-65 C0Z8A4.1 C0Z8A4_BREBN Glycerophosphoryl diester phosphodiesteras... 235 3e-60 Q2B374.1 Q2B374_9BACI Probable glycerophosphodiester phosphodies... 210 8e-53 B3K444.1 B3K444_9BACI Glycerophosphoryl diester phosphodiesterase 181 7e-44 >B3KLB3.1 B3KLB3_9BACI Glycerophosphodiester phosphodiesterase EC=3.1.4.46 Length = 257 Score = 319 bits (818), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 150/242 (61%), Positives = 185/242 (76%) Query: 4 MKNMCVAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTN 63 M N+CVAHRGFSGKAPENT AA++MA+ PFV WMEIDVQLT+DGVPVVIHDFS+DRTT Sbjct: 1 MNNLCVAHRGFSGKAPENTRAAIQMAMDEPFVHWMEIDVQLTQDGVPVVIHDFSVDRTTT 60 Query: 64 GHGKVKNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDMYPG 123 G GKVK++ ++ +RR+DAG WKGR FRGE+VPSL EVL+L GRL+LNIELKTSGDMYPG Sbjct: 61 GKGKVKDLTWQQIRRMDAGEWKGRQFRGEQVPSLDEVLQLVKGRLKLNIELKTSGDMYPG 120 Query: 124 LEQAVIDLVNAHGMRDEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPARKLKELDC 183 LE V++ + AH M +VVLTSF+ AL + KEL+P +TGLI D+ + +LK++ C Sbjct: 121 LEATVLERIQAHRMLPDVVLTSFEPKALMKAKELEPDVKTGLIIDAHPRNLLARLKQMKC 180 Query: 184 SFLSISFDRLNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPDIWGDTF 243 SFLSI + L+ A L + GI MAWTV+ AK MR L+ M+ IMICTNRPD WG F Sbjct: 181 SFLSIGYSHLDAAFASDLVKNGITPMAWTVDDAKSMRSLARMNPAIMICTNRPDTWGQIF 240 Query: 244 LE 245 + Sbjct: 241 IH 242 >B1DL22.1 B1DL22_9BACL Glycerophosphodiester phosphodiesterase EC=3.1.4.46 Length = 246 Score = 251 bits (641), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 125/237 (52%), Positives = 164/237 (69%), Gaps = 1/237 (0%) Query: 4 MKNMCVAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTN 63 M+N CVAHRG+SG+APENT+AA+++AI P + W+E+DVQL+KD VPVVIHD+ L RTTN Sbjct: 1 MRNACVAHRGWSGRAPENTIAAIQLAIDEPDIEWIELDVQLSKDNVPVVIHDYKLKRTTN 60 Query: 64 GHGKVKNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDMYPG 123 G G+VK++ ++ LDAGSW ++GE VP+L +VL+ A GR+RLNIELKT G YP Sbjct: 61 GRGEVKSLTAAELQALDAGSWFSPLYKGEGVPALSQVLDKARGRIRLNIELKTDGVRYPK 120 Query: 124 LEQAVIDLVNAHGMRDEVVLTSFDAGALQRIKELD-PRFRTGLIYDSRSGDPARKLKELD 182 LE V+ L+ +GM DEVV+TSF AG L +L RTGLI D +L++L Sbjct: 121 LEDYVVQLIKDYGMEDEVVITSFHAGTLFNTHKLTGGSIRTGLILDGWRNSLPVELQQLG 180 Query: 183 CSFLSISFDRLNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPDIW 239 FLSI RLN +LL + GI+VMAWT N + +R+L A+ E+MICTN PD W Sbjct: 181 AQFLSIEHGRLNASRLELLKQAGIQVMAWTPNDERSIRKLIALDPELMICTNYPDRW 237 >C0Z8A4.1 C0Z8A4_BREBN Glycerophosphoryl diester phosphodiesterase EC=3.1.4.46 Length = 239 Score = 235 bits (599), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 112/232 (48%), Positives = 156/232 (67%) Query: 6 NMCVAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTNGH 65 N+C+AHRG+SGKAPENT+ A+R+A+A P ++ MEIDVQLT+DGVPV+IHDF+L+RTTNG Sbjct: 2 NICMAHRGWSGKAPENTMTAIRLALAEPAIKAMEIDVQLTRDGVPVLIHDFTLERTTNGR 61 Query: 66 GKVKNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDMYPGLE 125 G V + +R LDAGSW G F GER+P+L+E L GR LNIELK + DMYP + Sbjct: 62 GLVMDRTLAELRELDAGSWFGDKFAGERIPTLEEALIAVKGRCTLNIELKATSDMYPDIA 121 Query: 126 QAVIDLVNAHGMRDEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPARKLKELDCSF 185 + V+ LV HGM+ EV LTSFD ++ ++ LD +TGLI R +++ + Sbjct: 122 EKVLALVEKHGMKQEVYLTSFDHDLIRHVRTLDQEVQTGLIVYGRPVLMLEQMEAAGATI 181 Query: 186 LSISFDRLNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPD 237 LS+ + LN L E+G +V+AWT++ K +R + + H ++ ICTN PD Sbjct: 182 LSMGYPFLNRELTVAAIEKGFKVIAWTLDDPKHIREVISWHPQVQICTNHPD 233 >Q2B374.1 Q2B374_9BACI Probable glycerophosphodiester phosphodiesterase Length = 249 Score = 210 bits (535), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 102/235 (43%), Positives = 150/235 (63%) Query: 4 MKNMCVAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTN 63 +KN CVAHRG+SGKAPENT AA R+A+ P + +E+DV L++DG+PVVIHD +L+RTTN Sbjct: 2 LKNACVAHRGWSGKAPENTAAAFRLALEHPMISAIELDVHLSRDGIPVVIHDHTLERTTN 61 Query: 64 GHGKVKNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDMYPG 123 G G VK+ + LDAGSW + GE + SL+E++++A G+ +L IELK MY G Sbjct: 62 GSGNVKDHTAAELALLDAGSWFSPEYSGETISSLEEIIQMAKGKKKLFIELKQMAGMYEG 121 Query: 124 LEQAVIDLVNAHGMRDEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPARKLKELDC 183 LE+ V L+ + + E ++ SFD +L ++KELDPR RTGLIY + A + Sbjct: 122 LEEKVAGLIRKYSLYKEALVISFDHHSLLKLKELDPRIRTGLIYLGHATLLAEQAAYTGA 181 Query: 184 SFLSISFDRLNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPDI 238 S +S+ + L+ GL + L + I + WTVN + ++++ + I I TN P+I Sbjct: 182 SHVSVHYGFLSRGLVESLLDARIEIGTWTVNGKEALKKVQDLGGSIQITTNHPEI 236 >B3K444.1 B3K444_9BACI Glycerophosphoryl diester phosphodiesterase Length = 239 Score = 181 bits (458), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 96/242 (39%), Positives = 144/242 (59%), Gaps = 9/242 (3%) Query: 4 MKNMCVAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTN 63 M+ +AHRG+ K PENT+ R A+ L F +E+DVQLTKDGVPVVIHD +++RTTN Sbjct: 1 MEVRGLAHRGYPLKFPENTMLGFRKALELGFSH-LELDVQLTKDGVPVVIHDPTVNRTTN 59 Query: 64 GHGKVKNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDMYPG 123 G G + E +R+LDAG RGE +P+L+E L A R+ ++IELK GD YPG Sbjct: 60 GTGAINAFTLEEIRKLDAG-------RGEVIPTLEEALLFAKDRMIVDIELKQMGDTYPG 112 Query: 124 LEQAVIDLVNAHGMRDEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPARKLKELDC 183 LEQ VID++ GM +V LTSFD +++R + LDP GL+ + L+++ Sbjct: 113 LEQKVIDVIKDTGMEKQVFLTSFDHYSIERARSLDPDIELGLVIYGATPSVFPYLEQIGA 172 Query: 184 SFLSISFDRLNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPDIWGDTF 243 ++S+ + + P + +RGI+++ WT + + L A + E++I TN + W + Sbjct: 173 RYVSVKYVYVTPSFMEEARKRGIQLIVWTPDDEAVLASL-AQYPELLIVTNNLEGWAKVY 231 Query: 244 LE 245 E Sbjct: 232 KE 233 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,463,429,634 Number of extensions: 58194512 Number of successful extensions: 150086 Number of sequences better than 1.0e-05: 2117 Number of HSP's gapped: 147101 Number of HSP's successfully gapped: 2120 Length of query: 246 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 114 Effective length of database: 1,817,122,223 Effective search space: 207151933422 Effective search space used: 207151933422 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)